BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30378
(707 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 32 0.092
SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 29 0.65
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 29 0.86
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 29 0.86
SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomy... 29 0.86
SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 28 1.1
SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 28 1.1
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.1
SPCC338.07c |||NatA N-acetyltransferase complex subunit |Schizos... 28 1.1
SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 28 1.5
SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 27 2.0
SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 27 2.6
SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 27 2.6
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 27 2.6
SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 27 3.5
SPCC364.01 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 27 3.5
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 27 3.5
SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 26 4.6
SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharom... 26 4.6
SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 26 4.6
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 8.0
>SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein
Pkl1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 832
Score = 31.9 bits (69), Expect = 0.092
Identities = 13/48 (27%), Positives = 26/48 (54%)
Frame = +1
Query: 358 NDAEAKVLELTHQLELKDSEIYQKTHEYTITLTQRNDEFENVRQQLVE 501
+D E+ L L L++++ +K HEY++ L + E +Q ++E
Sbjct: 171 DDIESAYLNLRSHLQVQEQVYAEKDHEYSLQLQSYREAAEKAKQDILE 218
>SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit
Mtr4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1117
Score = 29.1 bits (62), Expect = 0.65
Identities = 17/47 (36%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
Frame = +2
Query: 29 IVTMEQVTSLQSKLQDKEEH-LRNLQEKYADVINQLEILRSEIEDEK 166
I M++V L+S+L H L+EKYA+ + +L +L E++D K
Sbjct: 865 IKLMKKVNILESRLLSNPLHNFSELEEKYAEYLRKLALL-EEVKDLK 910
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1207
Score = 28.7 bits (61), Expect = 0.86
Identities = 11/44 (25%), Positives = 25/44 (56%)
Frame = +2
Query: 38 MEQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIEDEKV 169
MEQ+ + + Q+ EE +++ DV++++ L + +E+V
Sbjct: 833 MEQLAIERQEEQESEEEYEEVEQSLMDVVDEMSDLAESVPEEEV 876
>SPAC3A11.05c |kms1||meiotic spindle pole body protein
Kms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 607
Score = 28.7 bits (61), Expect = 0.86
Identities = 16/46 (34%), Positives = 26/46 (56%)
Frame = +1
Query: 355 VNDAEAKVLELTHQLELKDSEIYQKTHEYTITLTQRNDEFENVRQQ 492
V+ E KV ELTHQL + DS K +E + +N++ + ++ Q
Sbjct: 219 VDILEGKVNELTHQLNMADS----KYNESKVANNSQNNQIKTLKAQ 260
>SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 333
Score = 28.7 bits (61), Expect = 0.86
Identities = 10/35 (28%), Positives = 21/35 (60%)
Frame = +1
Query: 418 IYQKTHEYTITLTQRNDEFENVRQQLVEYESASKI 522
+ + H+Y + + +D E VRQ+L++ +A K+
Sbjct: 163 LISRPHDYFAEMLKSDDHMEKVRQELIKEATAKKL 197
>SPAC1786.03 |cut11|SPAC24C9.01|integral membrane
nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = -3
Query: 612 DSFGIGLIRIQIFMH-FQSVEWLIRTLFHKSNLRCAF 505
DS +I + F + FQ++ WLIR F KS + C F
Sbjct: 178 DSPSRPVINVSFFNYIFQNLGWLIRFSFRKSIICCLF 214
>SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein
3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1385
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/30 (36%), Positives = 22/30 (73%)
Frame = +2
Query: 80 EEHLRNLQEKYADVINQLEILRSEIEDEKV 169
E ++N+ EK+ +V +Q+E++RS++ KV
Sbjct: 1018 EREVKNVVEKFDEVNDQIELMRSDVLLRKV 1047
>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 547
Score = 28.3 bits (60), Expect = 1.1
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +2
Query: 50 TSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIEDEK 166
T L+S++ E L+ +K V +QLE L+ E+EK
Sbjct: 409 TDLRSRVDISESKLKKRDDKLKRVSSQLEHLKHNYEEEK 447
>SPCC338.07c |||NatA N-acetyltransferase complex subunit
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 729
Score = 28.3 bits (60), Expect = 1.1
Identities = 16/50 (32%), Positives = 29/50 (58%)
Frame = +2
Query: 8 TQEETHKIVTMEQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIE 157
TQEE+ ++ M V + ++D +HL ++++K D + LE R+E E
Sbjct: 184 TQEESEAMLFMNLVILKKDGVEDAYKHLLSIEKKVLDRVAFLE-TRAEYE 232
>SPCC364.06 |nap1||nucleosome assembly protein Nap1
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 393
Score = 27.9 bits (59), Expect = 1.5
Identities = 14/42 (33%), Positives = 25/42 (59%)
Frame = +2
Query: 32 VTMEQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIE 157
+T E V+ L +Q + LR LQ++Y+D+ +Q + E+E
Sbjct: 63 LTSEGVSELPEAVQRRISGLRGLQKRYSDLESQFQKELFELE 104
>SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr
1|||Manual
Length = 161
Score = 27.5 bits (58), Expect = 2.0
Identities = 12/39 (30%), Positives = 22/39 (56%)
Frame = +2
Query: 44 QVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIED 160
+V SL+ + D E+ L + +KY V +L+ + +ED
Sbjct: 122 RVQSLERERDDMEQKLEEMTDKYTKVKAELDEVHQALED 160
>SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1208
Score = 27.1 bits (57), Expect = 2.6
Identities = 17/45 (37%), Positives = 28/45 (62%), Gaps = 3/45 (6%)
Frame = +2
Query: 41 EQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRS---EIEDEK 166
E+ L+SKLQ E+ +L+ + + NQ+E LR+ EI++EK
Sbjct: 435 EERDVLESKLQTLEDDNNSLRLMTSSLGNQIESLRTQNREIDEEK 479
>SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1274
Score = 27.1 bits (57), Expect = 2.6
Identities = 21/57 (36%), Positives = 33/57 (57%), Gaps = 6/57 (10%)
Frame = +2
Query: 5 STQEET---HKIVTMEQV-TSLQSKL--QDKEEHLRNLQEKYADVINQLEILRSEIE 157
ST+E+T K + Q+ L SKL Q+KE+HL+ LQ Y N LE+ ++++
Sbjct: 1051 STKEDTKLGEKCANIVQLQVDLLSKLADQEKEKHLKYLQSSYK---NSLEVQLAKLD 1104
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/32 (31%), Positives = 22/32 (68%)
Frame = +2
Query: 71 QDKEEHLRNLQEKYADVINQLEILRSEIEDEK 166
+D +E L LQE+YA +I+++ + ++++ K
Sbjct: 3353 KDLDEKLLQLQEEYASMISEIHSMELQMDEVK 3384
>SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 527
Score = 26.6 bits (56), Expect = 3.5
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 53 SLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIEDE 163
+L+ KLQ E RN + + + V N+LE L S + +E
Sbjct: 85 NLEEKLQLTETKCRNAESEKSRVENELEDLTSSLFEE 121
>SPCC364.01 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 320
Score = 26.6 bits (56), Expect = 3.5
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +1
Query: 355 VNDAEAKVLELTHQLELKDSEI 420
+ND E K+ ++H L+LKD I
Sbjct: 251 LNDVELKIPAISHSLKLKDGSI 272
>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
Psm3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1194
Score = 26.6 bits (56), Expect = 3.5
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +1
Query: 352 RVNDAEAKVLELTHQLELKDSEIYQKTHEYT 444
R+ +A++ EL H LEL E Q +YT
Sbjct: 266 RIERIKAEITELNHSLELLRVEKQQNDEDYT 296
>SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 882
Score = 26.2 bits (55), Expect = 4.6
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = -1
Query: 530 ISQIFDALSYSTSCCL 483
I+QIFDA+SYS C+
Sbjct: 393 INQIFDAISYSKGSCV 408
>SPCC1235.10c |sec6||exocyst complex subunit
Sec6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 730
Score = 26.2 bits (55), Expect = 4.6
Identities = 12/24 (50%), Positives = 15/24 (62%)
Frame = -3
Query: 522 NLRCAFILHKLLSNIFELIISLRQ 451
NL+CA + L N FELI S R+
Sbjct: 485 NLKCAGFMDNTLLNTFELITSERE 508
>SPCC162.08c |nup211||nuclear pore complex associated
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1837
Score = 26.2 bits (55), Expect = 4.6
Identities = 13/42 (30%), Positives = 26/42 (61%), Gaps = 1/42 (2%)
Frame = +2
Query: 35 TMEQVTSL-QSKLQDKEEHLRNLQEKYADVINQLEILRSEIE 157
T EQ L +S++ +EE + +L+ + D+ ++E+L+ E E
Sbjct: 986 THEQFKHLVESEISTREEKITSLRSELLDLNKRVEVLKEEKE 1027
>SPAC19A8.08 |upf2||nonsense-mediated decay protein
Upf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1049
Score = 25.4 bits (53), Expect = 8.0
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -1
Query: 179 FQMQPFHLLFQTLKFLID*LHP 114
FQ+ PFH++F+ K I+ P
Sbjct: 552 FQLMPFHMVFECYKLCINEFTP 573
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,397,687
Number of Sequences: 5004
Number of extensions: 43331
Number of successful extensions: 186
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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