BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30378 (707 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 32 0.092 SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 29 0.65 SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 29 0.86 SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 29 0.86 SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomy... 29 0.86 SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 28 1.1 SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting pro... 28 1.1 SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.1 SPCC338.07c |||NatA N-acetyltransferase complex subunit |Schizos... 28 1.1 SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccha... 28 1.5 SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|ch... 27 2.0 SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 27 2.6 SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|c... 27 2.6 SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 27 2.6 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 27 3.5 SPCC364.01 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||... 27 3.5 SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch... 27 3.5 SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe... 26 4.6 SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharom... 26 4.6 SPCC162.08c |nup211||nuclear pore complex associated protein|Sch... 26 4.6 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 8.0 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 31.9 bits (69), Expect = 0.092 Identities = 13/48 (27%), Positives = 26/48 (54%) Frame = +1 Query: 358 NDAEAKVLELTHQLELKDSEIYQKTHEYTITLTQRNDEFENVRQQLVE 501 +D E+ L L L++++ +K HEY++ L + E +Q ++E Sbjct: 171 DDIESAYLNLRSHLQVQEQVYAEKDHEYSLQLQSYREAAEKAKQDILE 218 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 29.1 bits (62), Expect = 0.65 Identities = 17/47 (36%), Positives = 28/47 (59%), Gaps = 1/47 (2%) Frame = +2 Query: 29 IVTMEQVTSLQSKLQDKEEH-LRNLQEKYADVINQLEILRSEIEDEK 166 I M++V L+S+L H L+EKYA+ + +L +L E++D K Sbjct: 865 IKLMKKVNILESRLLSNPLHNFSELEEKYAEYLRKLALL-EEVKDLK 910 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 28.7 bits (61), Expect = 0.86 Identities = 11/44 (25%), Positives = 25/44 (56%) Frame = +2 Query: 38 MEQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIEDEKV 169 MEQ+ + + Q+ EE +++ DV++++ L + +E+V Sbjct: 833 MEQLAIERQEEQESEEEYEEVEQSLMDVVDEMSDLAESVPEEEV 876 >SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schizosaccharomyces pombe|chr 1|||Manual Length = 607 Score = 28.7 bits (61), Expect = 0.86 Identities = 16/46 (34%), Positives = 26/46 (56%) Frame = +1 Query: 355 VNDAEAKVLELTHQLELKDSEIYQKTHEYTITLTQRNDEFENVRQQ 492 V+ E KV ELTHQL + DS K +E + +N++ + ++ Q Sbjct: 219 VDILEGKVNELTHQLNMADS----KYNESKVANNSQNNQIKTLKAQ 260 >SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 333 Score = 28.7 bits (61), Expect = 0.86 Identities = 10/35 (28%), Positives = 21/35 (60%) Frame = +1 Query: 418 IYQKTHEYTITLTQRNDEFENVRQQLVEYESASKI 522 + + H+Y + + +D E VRQ+L++ +A K+ Sbjct: 163 LISRPHDYFAEMLKSDDHMEKVRQELIKEATAKKL 197 >SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 28.3 bits (60), Expect = 1.1 Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = -3 Query: 612 DSFGIGLIRIQIFMH-FQSVEWLIRTLFHKSNLRCAF 505 DS +I + F + FQ++ WLIR F KS + C F Sbjct: 178 DSPSRPVINVSFFNYIFQNLGWLIRFSFRKSIICCLF 214 >SPAC15A10.16 |bud6|aip3, fat1, SPAC15E1.01|actin interacting protein 3 homolog Bud6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1385 Score = 28.3 bits (60), Expect = 1.1 Identities = 11/30 (36%), Positives = 22/30 (73%) Frame = +2 Query: 80 EEHLRNLQEKYADVINQLEILRSEIEDEKV 169 E ++N+ EK+ +V +Q+E++RS++ KV Sbjct: 1018 EREVKNVVEKFDEVNDQIELMRSDVLLRKV 1047 >SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/39 (35%), Positives = 22/39 (56%) Frame = +2 Query: 50 TSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIEDEK 166 T L+S++ E L+ +K V +QLE L+ E+EK Sbjct: 409 TDLRSRVDISESKLKKRDDKLKRVSSQLEHLKHNYEEEK 447 >SPCC338.07c |||NatA N-acetyltransferase complex subunit |Schizosaccharomyces pombe|chr 3|||Manual Length = 729 Score = 28.3 bits (60), Expect = 1.1 Identities = 16/50 (32%), Positives = 29/50 (58%) Frame = +2 Query: 8 TQEETHKIVTMEQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIE 157 TQEE+ ++ M V + ++D +HL ++++K D + LE R+E E Sbjct: 184 TQEESEAMLFMNLVILKKDGVEDAYKHLLSIEKKVLDRVAFLE-TRAEYE 232 >SPCC364.06 |nap1||nucleosome assembly protein Nap1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 393 Score = 27.9 bits (59), Expect = 1.5 Identities = 14/42 (33%), Positives = 25/42 (59%) Frame = +2 Query: 32 VTMEQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIE 157 +T E V+ L +Q + LR LQ++Y+D+ +Q + E+E Sbjct: 63 LTSEGVSELPEAVQRRISGLRGLQKRYSDLESQFQKELFELE 104 >SPAC27F1.02c |cdc8|fus4|tropomyosin|Schizosaccharomyces pombe|chr 1|||Manual Length = 161 Score = 27.5 bits (58), Expect = 2.0 Identities = 12/39 (30%), Positives = 22/39 (56%) Frame = +2 Query: 44 QVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIED 160 +V SL+ + D E+ L + +KY V +L+ + +ED Sbjct: 122 RVQSLERERDDMEQKLEEMTDKYTKVKAELDEVHQALED 160 >SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1208 Score = 27.1 bits (57), Expect = 2.6 Identities = 17/45 (37%), Positives = 28/45 (62%), Gaps = 3/45 (6%) Frame = +2 Query: 41 EQVTSLQSKLQDKEEHLRNLQEKYADVINQLEILRS---EIEDEK 166 E+ L+SKLQ E+ +L+ + + NQ+E LR+ EI++EK Sbjct: 435 EERDVLESKLQTLEDDNNSLRLMTSSLGNQIESLRTQNREIDEEK 479 >SPAP8A3.12c |||tripeptidylpeptidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1274 Score = 27.1 bits (57), Expect = 2.6 Identities = 21/57 (36%), Positives = 33/57 (57%), Gaps = 6/57 (10%) Frame = +2 Query: 5 STQEET---HKIVTMEQV-TSLQSKL--QDKEEHLRNLQEKYADVINQLEILRSEIE 157 ST+E+T K + Q+ L SKL Q+KE+HL+ LQ Y N LE+ ++++ Sbjct: 1051 STKEDTKLGEKCANIVQLQVDLLSKLADQEKEKHLKYLQSSYK---NSLEVQLAKLD 1104 >SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccharomyces pombe|chr 1|||Manual Length = 4196 Score = 27.1 bits (57), Expect = 2.6 Identities = 10/32 (31%), Positives = 22/32 (68%) Frame = +2 Query: 71 QDKEEHLRNLQEKYADVINQLEILRSEIEDEK 166 +D +E L LQE+YA +I+++ + ++++ K Sbjct: 3353 KDLDEKLLQLQEEYASMISEIHSMELQMDEVK 3384 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 26.6 bits (56), Expect = 3.5 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = +2 Query: 53 SLQSKLQDKEEHLRNLQEKYADVINQLEILRSEIEDE 163 +L+ KLQ E RN + + + V N+LE L S + +E Sbjct: 85 NLEEKLQLTETKCRNAESEKSRVENELEDLTSSLFEE 121 >SPCC364.01 |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 320 Score = 26.6 bits (56), Expect = 3.5 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = +1 Query: 355 VNDAEAKVLELTHQLELKDSEI 420 +ND E K+ ++H L+LKD I Sbjct: 251 LNDVELKIPAISHSLKLKDGSI 272 >SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1194 Score = 26.6 bits (56), Expect = 3.5 Identities = 12/31 (38%), Positives = 17/31 (54%) Frame = +1 Query: 352 RVNDAEAKVLELTHQLELKDSEIYQKTHEYT 444 R+ +A++ EL H LEL E Q +YT Sbjct: 266 RIERIKAEITELNHSLELLRVEKQQNDEDYT 296 >SPBC1921.05 |ape2||aminopeptidase Ape2|Schizosaccharomyces pombe|chr 2|||Manual Length = 882 Score = 26.2 bits (55), Expect = 4.6 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = -1 Query: 530 ISQIFDALSYSTSCCL 483 I+QIFDA+SYS C+ Sbjct: 393 INQIFDAISYSKGSCV 408 >SPCC1235.10c |sec6||exocyst complex subunit Sec6|Schizosaccharomyces pombe|chr 3|||Manual Length = 730 Score = 26.2 bits (55), Expect = 4.6 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = -3 Query: 522 NLRCAFILHKLLSNIFELIISLRQ 451 NL+CA + L N FELI S R+ Sbjct: 485 NLKCAGFMDNTLLNTFELITSERE 508 >SPCC162.08c |nup211||nuclear pore complex associated protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1837 Score = 26.2 bits (55), Expect = 4.6 Identities = 13/42 (30%), Positives = 26/42 (61%), Gaps = 1/42 (2%) Frame = +2 Query: 35 TMEQVTSL-QSKLQDKEEHLRNLQEKYADVINQLEILRSEIE 157 T EQ L +S++ +EE + +L+ + D+ ++E+L+ E E Sbjct: 986 THEQFKHLVESEISTREEKITSLRSELLDLNKRVEVLKEEKE 1027 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.4 bits (53), Expect = 8.0 Identities = 9/22 (40%), Positives = 14/22 (63%) Frame = -1 Query: 179 FQMQPFHLLFQTLKFLID*LHP 114 FQ+ PFH++F+ K I+ P Sbjct: 552 FQLMPFHMVFECYKLCINEFTP 573 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,397,687 Number of Sequences: 5004 Number of extensions: 43331 Number of successful extensions: 186 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 164 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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