BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30349 (908 letters) Database: mosquito 2352 sequences; 563,979 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha ... 30 0.11 AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha ... 29 0.26 CR954257-12|CAJ14163.1| 1645|Anopheles gambiae putative cytoskel... 24 7.3 AJ970245-1|CAI96717.1| 134|Anopheles gambiae putative reverse t... 23 9.7 >AF313909-1|AAL99382.1| 1024|Anopheles gambiae collagen IV alpha 1 chain protein. Length = 1024 Score = 29.9 bits (64), Expect = 0.11 Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 2/38 (5%) Frame = -3 Query: 906 IPGTPGPPLKQGLRLPNSPTGSKPGTPSEEGL--EPGK 799 +PG PGPP GL P G + G E+G EPG+ Sbjct: 395 LPGIPGPPCVDGLPGAAGPVGPR-GYDGEKGFKGEPGR 431 Score = 27.9 bits (59), Expect = 0.45 Identities = 13/31 (41%), Positives = 20/31 (64%), Gaps = 4/31 (12%) Frame = +2 Query: 824 DGVPGLE----PVGEFGNLRPCFKGGPGVPG 904 +G+PG++ P+GE G+ P KG G+PG Sbjct: 523 NGLPGMKGDMGPLGEKGDACPVVKGEKGLPG 553 Score = 24.2 bits (50), Expect = 5.5 Identities = 14/36 (38%), Positives = 16/36 (44%), Gaps = 5/36 (13%) Frame = -3 Query: 900 GTPGPPLKQGLRLPNSPTGSK-----PGTPSEEGLE 808 GTPGPP G P G K PG P G++ Sbjct: 146 GTPGPPGYPGDVGPKGEPGPKGPAGHPGAPGRPGVD 181 Score = 23.4 bits (48), Expect = 9.7 Identities = 9/14 (64%), Positives = 10/14 (71%) Frame = -3 Query: 906 IPGTPGPPLKQGLR 865 IPG PG P + GLR Sbjct: 741 IPGAPGAPGEMGLR 754 >AY027891-1|AAK15783.1| 801|Anopheles gambiae collagen IV alpha 1 chain precursor protein. Length = 801 Score = 28.7 bits (61), Expect = 0.26 Identities = 17/36 (47%), Positives = 20/36 (55%), Gaps = 5/36 (13%) Frame = +2 Query: 815 PSSDGVPGLE-PVGEFGNL----RPCFKGGPGVPGI 907 P +DG+ GL P GE G RP KG PG PG+ Sbjct: 231 PGNDGLEGLPGPQGEVGPRGFPGRPGEKGVPGTPGV 266 Score = 24.2 bits (50), Expect = 5.5 Identities = 12/30 (40%), Positives = 14/30 (46%) Frame = -3 Query: 903 PGTPGPPLKQGLRLPNSPTGSKPGTPSEEG 814 PG PGP +G P P G G E+G Sbjct: 410 PGAPGPKGPRGYEGPQGPKG-MDGFDGEKG 438 Score = 24.2 bits (50), Expect = 5.5 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -3 Query: 900 GTPGPPLKQGLRLPNSPTGSKPGTPSE 820 G PGP + GL P P+G +PG +E Sbjct: 628 GEPGPKGEPGLLGPPGPSG-EPGRDAE 653 >CR954257-12|CAJ14163.1| 1645|Anopheles gambiae putative cytoskeletal structural protein protein. Length = 1645 Score = 23.8 bits (49), Expect = 7.3 Identities = 12/46 (26%), Positives = 24/46 (52%) Frame = +1 Query: 346 LKFKPLA*GLSKAPLHFVT*LVKGYCNFGTILLVSLNLCCAKQYFE 483 L+F+P G S+ LHF+ L K N + + +++ A + ++ Sbjct: 988 LEFRPKHQGPSEVQLHFLEMLTKAVHNLFQYMDIPVDVAVAHRLYD 1033 >AJ970245-1|CAI96717.1| 134|Anopheles gambiae putative reverse transcriptase protein. Length = 134 Score = 23.4 bits (48), Expect = 9.7 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = -3 Query: 636 DVIYHQ*QLRAFLR*NFKTQLLNNLYLCILSRYMSKNVFNN 514 D I+HQ L ++ NF + L+N + I +R ++ N+ Sbjct: 79 DSIWHQGLLSKLIKLNFPSYLINIVNSFISNRQNKVHILNS 119 Database: mosquito Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 563,979 Number of sequences in database: 2352 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 977,716 Number of Sequences: 2352 Number of extensions: 21200 Number of successful extensions: 40 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 40 length of database: 563,979 effective HSP length: 64 effective length of database: 413,451 effective search space used: 98401338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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