BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30341 (792 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 146 3e-36 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 86 6e-18 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 78 1e-15 SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 72 8e-14 SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 29 0.76 SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyce... 29 1.0 SPCC188.03 |cnd3||condensin subunit Cnd3 |Schizosaccharomyces po... 28 1.8 SPBC1198.06c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 27 2.3 SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 2.3 SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 3.1 SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|ch... 27 4.1 SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pomb... 26 5.4 SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 5.4 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 146 bits (354), Expect = 3e-36 Identities = 61/84 (72%), Positives = 73/84 (86%) Frame = +2 Query: 2 EIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPR 181 EIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYVPR Sbjct: 3 EIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPR 62 Query: 182 AILVDLEPGTMDSVRSGPFGQIFR 253 A+LVDLEPGTMD+V+SG FG +FR Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFR 86 Score = 130 bits (313), Expect = 3e-31 Identities = 73/175 (41%), Positives = 100/175 (57%), Gaps = 3/175 (1%) Frame = +1 Query: 256 DNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXX 435 DN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+E+E+CD LQGFQ Sbjct: 88 DNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGM 147 Query: 436 XXXXXXKIREEYPDRIMNTYSVVPCPK-YQTLSSNHTMRLSQFTS*LKTQTKTYCIRQRG 612 KIREEYPDR+M T+SV P PK T+ + LS ++ +T+CI Sbjct: 148 GTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQL-VENSDETFCI-DNE 205 Query: 613 SIRHLLPQSQTCPHPPTGEP*PLSFPSQCLVAPI--ALRFPGQLNADLRKIG*KM 771 ++ + + P + L+ ++A + + RFPG+LN+DLRK+ M Sbjct: 206 ALSSIFANTLKIKSPSYDD---LNHLVSAVMAGVTTSFRFPGELNSDLRKLAVNM 257 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 85.8 bits (203), Expect = 6e-18 Identities = 35/83 (42%), Positives = 58/83 (69%) Frame = +2 Query: 2 EIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVPR 181 EI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+PR Sbjct: 4 EIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPR 63 Query: 182 AILVDLEPGTMDSVRSGPFGQIF 250 AIL+DLEP ++++ S +G ++ Sbjct: 64 AILIDLEPRVVNNILSDTYGSLY 86 Score = 55.2 bits (127), Expect = 1e-08 Identities = 37/162 (22%), Positives = 68/162 (41%), Gaps = 2/162 (1%) Frame = +1 Query: 280 GAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKI 459 GAGNNWA G Y+ + + ++D++ +E++ D L+GF ++ Sbjct: 99 GAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERL 157 Query: 460 REEYPDRIMNTYSVVPCPKYQTLSSNHTMRLSQFTS*--LKTQTKTYCIRQRGSIRHLLP 633 + YP +I+ TYSV P Q++S + + L + + ++ H+ Sbjct: 158 NDRYPKKIIQTYSVFP--NSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAA 215 Query: 634 QSQTCPHPPTGEP*PLSFPSQCLVAPIALRFPGQLNADLRKI 759 +P + L + + LR+PG +N DL I Sbjct: 216 DRLHTQNPTFHQQNQL-VSTVMSASTTTLRYPGYMNNDLVSI 256 Score = 29.5 bits (63), Expect = 0.58 Identities = 12/23 (52%), Positives = 17/23 (73%) Frame = +3 Query: 516 VSDTVVEPYNATLSVHQLVENTD 584 VSD VV+PYN+ L++ +L N D Sbjct: 178 VSDVVVQPYNSLLALKRLTLNAD 200 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 78.2 bits (184), Expect = 1e-15 Identities = 36/85 (42%), Positives = 51/85 (60%), Gaps = 2/85 (2%) Frame = +2 Query: 2 EIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGKYV 175 EI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GKYV Sbjct: 3 EIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYV 62 Query: 176 PRAILVDLEPGTMDSVRSGPFGQIF 250 PR+I VDLEP +D VR+GP+ +F Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLF 87 Score = 60.5 bits (140), Expect = 3e-10 Identities = 29/90 (32%), Positives = 44/90 (48%) Frame = +1 Query: 256 DNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXX 435 + + G+ A NN+A+GHYT G ELVD V D +R+ +++C LQGF Sbjct: 90 EQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGF 149 Query: 436 XXXXXXKIREEYPDRIMNTYSVVPCPKYQT 525 ++ EY + +SV P P+ T Sbjct: 150 GALLLERLAMEYTKKSKLQFSVYPAPQVST 179 Score = 33.5 bits (73), Expect = 0.035 Identities = 13/27 (48%), Positives = 19/27 (70%) Frame = +3 Query: 504 PLPKVSDTVVEPYNATLSVHQLVENTD 584 P P+VS +VVEPYN+ L+ H ++ D Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLAD 199 Score = 29.5 bits (63), Expect = 0.58 Identities = 14/28 (50%), Positives = 17/28 (60%) Frame = +2 Query: 602 DNEALYDICFRNLKLVHTHLRVNLNHLV 685 DNE+ YDIC RNL + NLN L+ Sbjct: 205 DNESCYDICRRNLDIERPSYE-NLNRLI 231 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 72.1 bits (169), Expect = 8e-14 Identities = 34/89 (38%), Positives = 50/89 (56%), Gaps = 6/89 (6%) Frame = +2 Query: 2 EIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEASG 163 E++ + GQ G QIG WE+ EHGI P G H ++ + +++E Sbjct: 3 EVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSETGQ 62 Query: 164 GKYVPRAILVDLEPGTMDSVRSGPFGQIF 250 GK+VPR+I VDLEP +D VR+GP+ +F Sbjct: 63 GKFVPRSIYVDLEPNVIDQVRTGPYKDLF 91 Score = 61.3 bits (142), Expect = 2e-10 Identities = 29/90 (32%), Positives = 46/90 (51%) Frame = +1 Query: 256 DNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQXXXXXXXXXXXXX 435 + V G+ A NN+A+GHYT G E++DSVL+ +R+ +++C LQGF Sbjct: 94 EQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGL 153 Query: 436 XXXXXXKIREEYPDRIMNTYSVVPCPKYQT 525 ++ EY + +SV P P+ T Sbjct: 154 GALLLERLNMEYGKKSNLQFSVYPAPQVST 183 Score = 37.5 bits (83), Expect = 0.002 Identities = 14/27 (51%), Positives = 21/27 (77%) Frame = +3 Query: 504 PLPKVSDTVVEPYNATLSVHQLVENTD 584 P P+VS +VVEPYN+ L+ H ++N+D Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSD 203 Score = 30.3 bits (65), Expect = 0.33 Identities = 15/28 (53%), Positives = 17/28 (60%) Frame = +2 Query: 602 DNEALYDICFRNLKLVHTHLRVNLNHLV 685 DNEA YDIC RNL + NLN L+ Sbjct: 209 DNEACYDICRRNLDIERPTYE-NLNRLI 235 >SPAC30C2.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 29.1 bits (62), Expect = 0.76 Identities = 18/64 (28%), Positives = 28/64 (43%) Frame = +3 Query: 429 RYGHPPHLKDP*RVPRQNHEHILSSPLPKVSDTVVEPYNATLSVHQLVENTDENLLHSTT 608 R PP + P + N +LSSP SDT P + L++ V + ++H + Sbjct: 291 RLNSPPFITIPQSIKNTNSNFLLSSP-SLFSDTRTRPASYALALIITVPYEYDEIVHPVS 349 Query: 609 RLYT 620 YT Sbjct: 350 TYYT 353 >SPCC1494.10 ||SPCC70.01|transcription factor |Schizosaccharomyces pombe|chr 3|||Manual Length = 964 Score = 28.7 bits (61), Expect = 1.0 Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 2/57 (3%) Frame = +1 Query: 598 IRQRGSIRHL-LPQSQT-CPHPPTGEP*PLSFPSQCLVAPIALRFPGQLNADLRKIG 762 +R G +RH LP S HPP G+ PSQ L L+ PG ++++G Sbjct: 415 MRNFGDVRHQKLPTSSPPSQHPPVGQ-----IPSQYLPYQAGLKVPGNTPIPVKQVG 466 >SPCC188.03 |cnd3||condensin subunit Cnd3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 875 Score = 27.9 bits (59), Expect = 1.8 Identities = 15/43 (34%), Positives = 23/43 (53%) Frame = +3 Query: 522 DTVVEPYNATLSVHQLVENTDENLLHSTTRLYTTSASAISNLS 650 D VE A + +Q +EN D NLL RL ++ S ++ L+ Sbjct: 239 DESVEKAAADMLAYQWIENADNNLLELLERLDVSNNSDVAVLA 281 >SPBC1198.06c |||mannan endo-1,6-alpha-mannosidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 27.5 bits (58), Expect = 2.3 Identities = 20/57 (35%), Positives = 27/57 (47%) Frame = -1 Query: 717 GNWCHQTL*GKTKWLRFTRRWVWTSLRLRKQMSYRASLSNAVGFRLCFQLAGELRES 547 G W T G +W F W+ SY+AS+SNA+ F+L +LA ES Sbjct: 150 GRWDSSTCGGGLRWQAFA--WL-------NGYSYKASVSNALLFQLSSRLARFTNES 197 >SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual Length = 234 Score = 27.5 bits (58), Expect = 2.3 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +1 Query: 298 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 387 A+GH G ELV + D +RK+SE+ L+ Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212 >SPBC19G7.16 |iws1||transcription elongation factor complex subunit Iws1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 428 Score = 27.1 bits (57), Expect = 3.1 Identities = 10/34 (29%), Positives = 22/34 (64%) Frame = +2 Query: 35 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 136 N++G E+++++ +DPT A + DLQ++ + Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166 >SPAC1B3.15c |||membrane transporter|Schizosaccharomyces pombe|chr 1|||Manual Length = 628 Score = 26.6 bits (56), Expect = 4.1 Identities = 19/54 (35%), Positives = 27/54 (50%) Frame = +2 Query: 407 PSVAAPGPVWAPSSSQRSVKSTPTES*THTQ*SPAQSIRHCRRTIQCDSLSSPA 568 PS+A+ P P SSQ S S P S + S A SI ++ + ++SS A Sbjct: 14 PSIASSFPTVKPFSSQNSTTSNPELSHIEKE-SNASSIIISQQPLSPSNISSAA 66 >SPAC922.06 |||short chain dehydrogenase|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 26.2 bits (55), Expect = 5.4 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = +2 Query: 371 LAIAYRASNLHIPSVAAPGPVWAPSSSQRSVK 466 LA+ Y + + +V APG +W+P+ +R K Sbjct: 159 LAVRYGPLGIRV-NVCAPGTIWSPAWDERFKK 189 >SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 682 Score = 26.2 bits (55), Expect = 5.4 Identities = 15/43 (34%), Positives = 26/43 (60%) Frame = +3 Query: 450 LKDP*RVPRQNHEHILSSPLPKVSDTVVEPYNATLSVHQLVEN 578 +++P +P+QNHEH SPL + SD + + L V+ V++ Sbjct: 641 VQNPLLMPQQNHEH---SPLVR-SDAALHDHGPLLPVYPDVDS 679 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,137,721 Number of Sequences: 5004 Number of extensions: 62454 Number of successful extensions: 202 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 177 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 196 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 385381248 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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