Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30338
(730 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g63700.1 68414.m07209 protein kinase, putative contains prote... 35 0.048
At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC... 32 0.34
At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC... 32 0.34
At3g63400.2 68416.m07138 peptidyl-prolyl cis-trans isomerase cyc... 30 1.8
At1g68725.1 68414.m07853 arabinogalactan-protein, putative (AGP1... 30 1.8
At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro... 29 3.2
At5g42720.1 68418.m05203 glycosyl hydrolase family 17 protein si... 29 4.2
At4g02330.1 68417.m00317 pectinesterase family protein contains ... 29 4.2
At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related... 29 4.2
At5g23080.1 68418.m02698 SWAP (Suppressor-of-White-APricot)/surp... 28 5.5
At4g04070.1 68417.m00576 hypothetical protein low similarity to ... 28 7.3
At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,... 28 7.3
At1g49010.1 68414.m05495 myb family transcription factor contain... 28 7.3
At4g30460.1 68417.m04325 glycine-rich protein 27 9.6
At3g60320.1 68416.m06742 expressed protein contains Pfam profile... 27 9.6
At1g54610.1 68414.m06228 protein kinase family protein contains ... 27 9.6
At1g43880.1 68414.m05055 hypothetical protein low similarity to ... 27 9.6
At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 27 9.6
>At1g63700.1 68414.m07209 protein kinase, putative contains protein
kinase domain, Pfam:PF00069; similar to MEK kinase
(MAP3Ka) [Arabidopsis thaliana] gi|4204912|gb|AAD10848
Length = 883
Score = 35.1 bits (77), Expect = 0.048
Identities = 34/123 (27%), Positives = 51/123 (41%), Gaps = 3/123 (2%)
Frame = +1
Query: 199 PINSPARSRMNDTESS-GTAADNLLAVVQSTSTGQRPKARSARTSAHHSPAPALRPAA-- 369
P SP RS + D S+ G + V +GQ S S ++S +
Sbjct: 239 PSRSPMRSFIPDQVSNHGLLISKPYSDVSLLGSGQCSSPGSGYNSGNNSIGGDMATQLFW 298
Query: 370 QNYE*SPALRPASLTPRHSLPVPVQKLLAADASPSHRRQPDSTTGNQ*RAAHKHRQEEAH 549
SP P +PR + P P ++ + +P H R STTG+ R +RQ ++H
Sbjct: 299 PQSRCSPECSPVP-SPRMTSPGPSSRIQSGAVTPLHPRAGGSTTGSPTRRLDDNRQ-QSH 356
Query: 550 RRP 558
R P
Sbjct: 357 RLP 359
>At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 32.3 bits (70), Expect = 0.34
Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = +1
Query: 280 QSTSTGQRPKARSARTSAHHSP-APALRPAAQNYE*SPALRPASLTPRHSLPVPVQKLLA 456
+ S +R RS SP +PAL+ A+ + E SP R +P P P++K+ A
Sbjct: 233 REVSPDKRSNERSPSPRRSLSPRSPALQKASPSKEMSPERRSNERSPSPGSPAPLRKVDA 292
Query: 457 ADASPS 474
A S S
Sbjct: 293 ASRSQS 298
>At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 32.3 bits (70), Expect = 0.34
Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Frame = +1
Query: 280 QSTSTGQRPKARSARTSAHHSP-APALRPAAQNYE*SPALRPASLTPRHSLPVPVQKLLA 456
+ S +R RS SP +PAL+ A+ + E SP R +P P P++K+ A
Sbjct: 233 REVSPDKRSNERSPSPRRSLSPRSPALQKASPSKEMSPERRSNERSPSPGSPAPLRKVDA 292
Query: 457 ADASPS 474
A S S
Sbjct: 293 ASRSQS 298
>At3g63400.2 68416.m07138 peptidyl-prolyl cis-trans isomerase
cyclophilin-type family protein similar to cyclophylin
[Digitalis lanata] GI:1563719; contains Pfam profile
PF00160: peptidyl-prolyl cis-trans isomerase,
cyclophilin-type; contains AT-donor splice site at
intron 9
Length = 387
Score = 29.9 bits (64), Expect = 1.8
Identities = 30/104 (28%), Positives = 43/104 (41%), Gaps = 1/104 (0%)
Frame = +1
Query: 196 RPINSPARSRMNDTESSGTAADNLLAVVQSTSTGQRPKARSARTSAHHSPAPALR-PAAQ 372
RP P+ + +DTESS ++ D ++ S Q P+ RS S +P R P +Q
Sbjct: 291 RPDRKPSTNSSSDTESSSSSDDGYRRRLRDGSRSQSPRHRS------RSQSPRKRQPISQ 344
Query: 373 NYE*SPALRPASLTPRHSLPVPVQKLLAADASPSHRRQPDSTTG 504
+ + L P R P + L SPSH P G
Sbjct: 345 DLK--SRLGPQRSPIRGGRTSPAESL-----SPSHSPSPPGKRG 381
>At1g68725.1 68414.m07853 arabinogalactan-protein, putative (AGP19)
non-consensus splice site at the intron:exon boundary
(AT:exon)
Length = 247
Score = 29.9 bits (64), Expect = 1.8
Identities = 32/139 (23%), Positives = 48/139 (34%), Gaps = 3/139 (2%)
Frame = +1
Query: 163 PTVICKP*NTPRPINSPARSRMNDTESSGTAADNLLAVVQSTSTGQRPKARSARTSAHHS 342
PT P + +P SP T +S A + +T Q P++ A
Sbjct: 54 PTTTTPPVSAAQPPASPVTPPPAVTPTSPPAPKVAPVISPATPPPQPPQSPPASAPTVSP 113
Query: 343 PAPALRPAAQNYE*SPAL---RPASLTPRHSLPVPVQKLLAADASPSHRRQPDSTTGNQ* 513
P + PA + +PA PAS P + P P +PS P +
Sbjct: 114 PPVSPPPAPTSPPPTPASPPPAPASPPPAPASPPPAPVSPPPVQAPSPISLPPAPAPAPT 173
Query: 514 RAAHKHRQEEAHRRPDAVP 570
+ KH+ + H P P
Sbjct: 174 KHKRKHKHKRHHHAPAPAP 192
>At5g53870.1 68418.m06701 plastocyanin-like domain-containing
protein contains similarity to SP|Q02917 Early nodulin
55-2 precursor {Glycine max}; PF02298: Plastocyanin-like
domain
Length = 370
Score = 29.1 bits (62), Expect = 3.2
Identities = 29/105 (27%), Positives = 40/105 (38%), Gaps = 4/105 (3%)
Frame = +1
Query: 199 PINSPARSRMNDTESSGTAADNLLAVVQSTSTGQRPKARSARTSAHHSPAPALRPAAQNY 378
P +SPA + + S A + A S S P + S S HSPA P+ +
Sbjct: 229 PSHSPAHT---PSHSPAHAPSHSPAHAPSHSPAHAP-SHSPAHSPSHSPATPKSPSPSSS 284
Query: 379 E*SPALRPASLTPRHSLPV----PVQKLLAADASPSHRRQPDSTT 501
P+ +TP+ PV P Q +D S P TT
Sbjct: 285 PAQSPATPSPMTPQSPSPVSSPSPDQSAAPSDQSTPLAPSPSETT 329
>At5g42720.1 68418.m05203 glycosyl hydrolase family 17 protein
similar to glucan endo-1,3-beta-glucosidase precursor
SP:P52409 from [Triticum aestivum]
Length = 438
Score = 28.7 bits (61), Expect = 4.2
Identities = 13/40 (32%), Positives = 20/40 (50%)
Frame = +1
Query: 388 PALRPASLTPRHSLPVPVQKLLAADASPSHRRQPDSTTGN 507
P L PA+ R +LP P Q +L + +PS + + N
Sbjct: 365 PRLPPAAAPTRQTLPSPPQMILPSPVTPSDKNSGQTDVHN 404
>At4g02330.1 68417.m00317 pectinesterase family protein contains
Pfam profile: PF01095 pectinesterase
Length = 573
Score = 28.7 bits (61), Expect = 4.2
Identities = 18/58 (31%), Positives = 25/58 (43%), Gaps = 1/58 (1%)
Frame = -3
Query: 566 TASGRRWASS*RCLWAARYWLPVVES-GWRRWLGDASAASNFCTGTGSEWRGVSDAGR 396
T GR W R ++ Y VVE GWR W GD + ++ + + G S R
Sbjct: 474 TYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDR 531
>At3g23670.1 68416.m02976 phragmoplast-associated kinesin-related
protein, putative similar to kinesin like protein
GB:CAB10194 from [Arabidopsis thaliana]
Length = 1313
Score = 28.7 bits (61), Expect = 4.2
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = +1
Query: 313 RSARTSAHHSPAPALRPAAQNYE*SPALRPASLTPRHSLPVPVQKLLAADAS 468
R ++S ++P P L ++ SPA + L PR P+++ L A+A+
Sbjct: 32 RKIKSSKENAPPPDLNSLIPDHRSSPAKLKSPLPPRPPSSNPLKRKLIAEAT 83
>At5g23080.1 68418.m02698 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 930
Score = 28.3 bits (60), Expect = 5.5
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 520 AHKHRQEEAHRRPDAVPG 573
+ KH ++E H RP A+PG
Sbjct: 131 SRKHAEKEQHERPSAIPG 148
>At4g04070.1 68417.m00576 hypothetical protein low similarity to
protective antigen [Streptococcus pyogenes] GI:8996050,
fibrinogen-binding protein [Streptococcus equi]
GI:3093478
Length = 728
Score = 27.9 bits (59), Expect = 7.3
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +1
Query: 205 NSPARSRMNDTESSGTAADNLLAVVQSTSTGQRPKARSART 327
N+ A +R +D ++ TAA N LAV ++++ P T
Sbjct: 278 NAEAHARADDLSAAVTAARNTLAVSNASASASHPSLPEVNT 318
>At1g77410.1 68414.m09015 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase SP:P45582 from
[Asparagus officinalis]
Length = 815
Score = 27.9 bits (59), Expect = 7.3
Identities = 20/70 (28%), Positives = 28/70 (40%), Gaps = 6/70 (8%)
Frame = +1
Query: 148 LATAIPTVICKP*NTPRPINSPARSRMNDTESSGTAADNLLAVVQSTST------GQRPK 309
L T +P V+CK + P P+ + R G + N A+ T G+ P
Sbjct: 207 LDTGVPWVMCKQDDAPDPLVNACNGRQCGETFKGPNSPNKPAIWTENWTSFYQTYGEEPL 266
Query: 310 ARSARTSAHH 339
RSA A H
Sbjct: 267 IRSAEDIAFH 276
>At1g49010.1 68414.m05495 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 314
Score = 27.9 bits (59), Expect = 7.3
Identities = 21/89 (23%), Positives = 33/89 (37%), Gaps = 7/89 (7%)
Frame = +2
Query: 464 HRPAIDASQTQQQVTSNGPP----INIVRKKPIGVQMLYQVGTVSMLLYTRTIIPDKDDA 631
HRPA Q Q Q + PP + + P+G ++ + + T ++P
Sbjct: 220 HRPAQPQPQPQPQPQQHHPPTMAGLGMYGGAPVGQPIIAPPDHMGSAVGTPVMLPPPMGT 279
Query: 632 KNHRH---LSATAYAIPVTAFSALQATKP 709
+H H L YA+P L P
Sbjct: 280 HHHHHHHHLGVAPYAVPAYPVPPLPQQHP 308
>At4g30460.1 68417.m04325 glycine-rich protein
Length = 162
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = -3
Query: 470 GDASAASNFCTGTGSEWRGVSDAGRRAGDYS*FCAAGRSAGAG 342
G +S++S+ + + S G DAG AG Y AG AG+G
Sbjct: 69 GSSSSSSSSSSSSSSSGGGGGDAGSEAGSY-----AGSHAGSG 106
>At3g60320.1 68416.m06742 expressed protein contains Pfam profiles:
PF04782: protein of unknown function (DUF632), PF04783:
protein of unknown function (DUF630)
Length = 796
Score = 27.5 bits (58), Expect = 9.6
Identities = 23/76 (30%), Positives = 35/76 (46%), Gaps = 4/76 (5%)
Frame = +1
Query: 193 PRPINSPARSRMNDTESSGTAADNLLAVVQSTSTG----QRPKARSARTSAHHSPAPALR 360
PR SPA S + +S + A + V STS+ Q+PK R + SP+ + R
Sbjct: 90 PRFSPSPAPSSVYPPSTSPSVASSKQPSVMSTSSNRRRKQQPKPRLPHILSESSPSSSPR 149
Query: 361 PAAQNYE*SPALRPAS 408
N+ P L P++
Sbjct: 150 SERSNF--MPNLYPSA 163
>At1g54610.1 68414.m06228 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 572
Score = 27.5 bits (58), Expect = 9.6
Identities = 20/51 (39%), Positives = 26/51 (50%), Gaps = 1/51 (1%)
Frame = +2
Query: 182 HRIRRDRLIPLPEAE*MIQ-KVAEPRLITF*LWCNPQAQGNGQKHDPPGHQ 331
HR R +R +P PEA +Q V RLIT + A+ +K PP HQ
Sbjct: 451 HRDRSNRALPAPEANAELQSNVDRRRLIT-----HANAKSKSEKFPPP-HQ 495
>At1g43880.1 68414.m05055 hypothetical protein low similarity to
protective antigen [Streptococcus pyogenes] GI:8996050,
fibrinogen-binding protein [Streptococcus equi]
GI:3093478
Length = 409
Score = 27.5 bits (58), Expect = 9.6
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +1
Query: 205 NSPARSRMNDTESSGTAADNLLAVVQSTSTGQRPKARSART 327
N+ A +R D ++ TAA N+LAV ++++ P T
Sbjct: 12 NAGALARAGDLSAAVTAAQNILAVSSASASTSHPSLPEVNT 52
>At1g21630.1 68414.m02708 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain; ESTs gb|T44428 and gb|AA395440 come from this
gene
Length = 1218
Score = 27.5 bits (58), Expect = 9.6
Identities = 22/80 (27%), Positives = 32/80 (40%)
Frame = +1
Query: 187 NTPRPINSPARSRMNDTESSGTAADNLLAVVQSTSTGQRPKARSARTSAHHSPAPALRPA 366
N PRP N P S S G + + + S ST + ++A+H P + PA
Sbjct: 184 NAPRPANQPMPSDWLSGRSVGPSGNVNSQIPSSQST---YGLTAPNSTANHITKPHITPA 240
Query: 367 AQNYE*SPALRPASLTPRHS 426
+ S RP P H+
Sbjct: 241 VTS---STTTRPQESAPVHN 257
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,414,473
Number of Sequences: 28952
Number of extensions: 394790
Number of successful extensions: 1072
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1028
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1065
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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