Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30333
(823 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 180 2e-46
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 178 7e-46
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 106 4e-24
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 77 4e-15
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.4
SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C |Schizosacchar... 27 4.3
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.4
SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1 |Schizosacch... 26 7.4
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.4
SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|... 26 7.4
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.8
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 25 9.8
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 180 bits (438), Expect = 2e-46
Identities = 75/112 (66%), Positives = 98/112 (87%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 434
+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR+
Sbjct: 69 SIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRR 128
Query: 435 LADQCTGLQGFLIFHSFGGGTGSGLLPY*WSVSSVDYGKKSKLEFAIYPAPQ 590
+AD C+GLQGFL+FHSFGGGTGSGL +++YGKKS L+F++YPAPQ
Sbjct: 129 MADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQ 180
Score = 97.1 bits (231), Expect = 3e-21
Identities = 49/69 (71%), Positives = 51/69 (73%), Gaps = 5/69 (7%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 228
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 229 TGAGKHVPR 255
TG GK VPR
Sbjct: 60 TGQGKFVPR 68
Score = 35.9 bits (79), Expect = 0.007
Identities = 26/67 (38%), Positives = 33/67 (49%)
Frame = +2
Query: 512 SLLMERLFR*LRQEV*TGVRHLPRASVSTAVVEPYNSILHHPHNP*GTLDCAFMGRQ*SH 691
+LL+ERL ++ P VST+VVEPYNS+L H DC FM
Sbjct: 155 ALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVL-TTHATLDNSDCTFMVDN-EA 212
Query: 692 LWTICRR 712
+ ICRR
Sbjct: 213 CYDICRR 219
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 178 bits (434), Expect = 7e-46
Identities = 77/112 (68%), Positives = 96/112 (85%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 434
+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR+
Sbjct: 65 SIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRR 124
Query: 435 LADQCTGLQGFLIFHSFGGGTGSGLLPY*WSVSSVDYGKKSKLEFAIYPAPQ 590
+AD C+GLQGFL+FHSFGGGTGSG +++Y KKSKL+F++YPAPQ
Sbjct: 125 IADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQ 176
Score = 104 bits (249), Expect = 2e-23
Identities = 45/64 (70%), Positives = 50/64 (78%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 243
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 244 HVPR 255
+VPR
Sbjct: 61 YVPR 64
Score = 37.5 bits (83), Expect = 0.002
Identities = 27/67 (40%), Positives = 34/67 (50%)
Frame = +2
Query: 512 SLLMERLFR*LRQEV*TGVRHLPRASVSTAVVEPYNSILHHPHNP*GTLDCAFMGRQ*SH 691
+LL+ERL ++ P VST+VVEPYNS+L H DC FM S
Sbjct: 151 ALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVL-TTHATLDLADCTFMVDNES- 208
Query: 692 LWTICRR 712
+ ICRR
Sbjct: 209 CYDICRR 215
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 106 bits (254), Expect = 4e-24
Identities = 50/112 (44%), Positives = 70/112 (62%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRK 434
AV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+
Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122
Query: 435 LADQCTGLQGFLIFHSFGGGTGSGLLPY*WSVSSVDYGKKSKLEFAIYPAPQ 590
A+ C LQGF + HS GGGTGSG+ S +Y + F++ PAP+
Sbjct: 123 EAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174
Score = 46.4 bits (105), Expect = 5e-06
Identities = 23/64 (35%), Positives = 31/64 (48%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 243
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 244 HVPR 255
+VPR
Sbjct: 59 YVPR 62
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 76.6 bits (180), Expect = 4e-15
Identities = 41/114 (35%), Positives = 65/114 (57%), Gaps = 2/114 (1%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRI 428
A+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I
Sbjct: 64 AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMI 122
Query: 429 RKLADQCTGLQGFLIFHSFGGGTGSGLLPY*WSVSSVDYGKKSKLEFAIYPAPQ 590
+ AD L+GF + HS GGTGSGL + + Y KK ++++P Q
Sbjct: 123 DREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQ 176
Score = 54.4 bits (125), Expect = 2e-08
Identities = 26/63 (41%), Positives = 38/63 (60%)
Frame = +1
Query: 67 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 246
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 247 VPR 255
+PR
Sbjct: 61 IPR 63
Score = 25.4 bits (53), Expect = 9.8
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = +2
Query: 512 SLLMERLFR*LRQEV*TGVRHLPRA-SVSTAVVEPYNSIL 628
S L+ERL +++ P + SVS VV+PYNS+L
Sbjct: 151 SFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLL 190
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 28.3 bits (60), Expect = 1.4
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -3
Query: 299 ADLINNSRFKIDEDSRGTCLPAPVSLKKVLKESSPPPM 186
AD+IN SR+ ID S T + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPAC17G8.14c |pck1|SPAC22H10.01c|protein kinase C
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 988
Score = 26.6 bits (56), Expect = 4.3
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +1
Query: 427 SASSLTSVPVCKDS*SSTPSVEVPALGYFLIDGASLPLTTARSLNWSSPSTPR 585
+ S L P S + TPS P L +S L++++ N S P +PR
Sbjct: 600 NTSPLPKTPEKDRSLNVTPSSSTPTPASVLAPPSSASLSSSKDANRSVPESPR 652
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 143 STASSLMARCPQTRPSGVETILSTLSSARPELAST 247
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPBC3B8.01c |arh1||NADPH-adrenodoxin reductase Arh1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 469
Score = 25.8 bits (54), Expect = 7.4
Identities = 16/64 (25%), Positives = 31/64 (48%), Gaps = 6/64 (9%)
Frame = -3
Query: 341 NKLFWMKQLSVCASADLINNSRFKI------DEDSRGTCLPAPVSLKKVLKESSPPPMVL 180
N F + + + + N RF+I D +S+ T +PA + ++ + +S P P +
Sbjct: 287 NLEFGLTPVEILGHKGNVENVRFQITDSIRTDAESKFTTIPAQLFIRSIGYKSMPLPGMK 346
Query: 179 SVGI 168
VG+
Sbjct: 347 DVGV 350
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 162 WPDAHRQDHRGWRRFFQHFLQR 227
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC17G6.17 |pof8||F-box protein Pof8|Schizosaccharomyces pombe|chr
1|||Manual
Length = 402
Score = 25.8 bits (54), Expect = 7.4
Identities = 17/57 (29%), Positives = 33/57 (57%), Gaps = 5/57 (8%)
Frame = -2
Query: 594 ETEARGRWRTPV*T-SC----RSQRKRRSINKEVTQSRYLHRRSGRSGILADRYTGQ 439
ETEA RW+TP+ +C R+Q ++++ + ++ R + S R ++A+ TG+
Sbjct: 337 ETEAIIRWKTPLHAETCINAFRTQERKQNSHDDIRAHR--KKGSSRPFLIAELITGE 391
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 53 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 175
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 629
Score = 25.4 bits (53), Expect = 9.8
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +1
Query: 511 FLIDGASLPLTTARSLNWSSPSTP 582
+L +GA L L T+ S++W TP
Sbjct: 225 YLPEGADLTLVTSYSVSWKYSDTP 248
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,664,200
Number of Sequences: 5004
Number of extensions: 81275
Number of successful extensions: 289
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 222
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 284
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 402440190
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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