Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30327
(595 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 139 3e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 96 4e-21
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 89 6e-19
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 75 1e-14
SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence orphan|Schizos... 29 0.39
SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr ... 27 2.1
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 27 2.7
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 4.8
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 8.3
SPAC644.07 |||Rieske ISP assembly protein|Schizosaccharomyces po... 25 8.3
SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces pomb... 25 8.3
SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|... 25 8.3
SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 25 8.3
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 139 bits (336), Expect = 3e-34
Identities = 64/84 (76%), Positives = 73/84 (86%)
Frame = +2
Query: 2 SDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSG 181
SDTVVEPYNATLS+HQLVEN+DET+CIDNEAL I TLK+ +P+Y DLNHLVS M+G
Sbjct: 176 SDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAG 235
Query: 182 VTTCLRFPGQLNADLRKLAVNMVP 253
VTT RFPG+LN+DLRKLAVNMVP
Sbjct: 236 VTTSFRFPGELNSDLRKLAVNMVP 259
Score = 124 bits (300), Expect = 7e-30
Identities = 55/77 (71%), Positives = 67/77 (87%)
Frame = +1
Query: 256 PRLHFFMPGFAPLTSRGSRQYRALTVPELTQQMFDAKNMMAACDPRHGRYLSVAAIFRGR 435
PRLHFFM GFAPL + GS ++A++VPELTQQMFDA NMM A DPRHGRYL+VAA+FRG+
Sbjct: 261 PRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGK 320
Query: 436 MSMKEVDEQMLNIQNKN 486
+SMKEVDEQ+ ++Q KN
Sbjct: 321 VSMKEVDEQIRSVQTKN 337
Score = 32.3 bits (70), Expect = 0.055
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +2
Query: 476 RTRTSSYFVEWIPQQT*RTACGEIPP 553
+T+ S+YFVEWIP + C +PP
Sbjct: 334 QTKNSAYFVEWIPDNVLKAVC-SVPP 358
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 95.9 bits (228), Expect = 4e-21
Identities = 41/84 (48%), Positives = 57/84 (67%)
Frame = +2
Query: 2 SDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSG 181
S +VVEPYN+ L+ H ++N+D T+ +DNEA YDIC R L + PTY +LN L++ +S
Sbjct: 182 STSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSS 241
Query: 182 VTTCLRFPGQLNADLRKLAVNMVP 253
+T LRF G LN DL + N+VP
Sbjct: 242 ITASLRFAGSLNVDLNEFQTNLVP 265
Score = 60.5 bits (140), Expect = 2e-10
Identities = 24/76 (31%), Positives = 46/76 (60%)
Frame = +1
Query: 256 PRLHFFMPGFAPLTSRGSRQYRALTVPELTQQMFDAKNMMAACDPRHGRYLSVAAIFRGR 435
PR+HF + ++P+ S + + +V E+T Q F+ N M CDPR GRY++ ++RG
Sbjct: 267 PRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLLYRGD 326
Query: 436 MSMKEVDEQMLNIQNK 483
+ ++V + +I+++
Sbjct: 327 VIPRDVQAAVTSIKSR 342
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 88.6 bits (210), Expect = 6e-19
Identities = 38/84 (45%), Positives = 55/84 (65%)
Frame = +2
Query: 2 SDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSG 181
S +VVEPYN+ L+ H ++ D T+ +DNE+ YDIC R L + P+Y +LN L++ +S
Sbjct: 178 STSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSS 237
Query: 182 VTTCLRFPGQLNADLRKLAVNMVP 253
+T LRF G LN DL + N+VP
Sbjct: 238 ITASLRFEGSLNVDLAEFQTNLVP 261
Score = 61.3 bits (142), Expect = 1e-10
Identities = 26/76 (34%), Positives = 44/76 (57%)
Frame = +1
Query: 256 PRLHFFMPGFAPLTSRGSRQYRALTVPELTQQMFDAKNMMAACDPRHGRYLSVAAIFRGR 435
PR+HF + +AP+ S + + +V E+T Q F+ N M CDPR GRY++ ++RG
Sbjct: 263 PRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLLYRGD 322
Query: 436 MSMKEVDEQMLNIQNK 483
+ ++V + I+ K
Sbjct: 323 VIPRDVQAAVTTIKAK 338
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 74.5 bits (175), Expect = 1e-14
Identities = 36/85 (42%), Positives = 49/85 (57%)
Frame = +2
Query: 2 SDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSG 181
SD VV+PYN+ L++ +L N D +DN AL I L PT+ N LVS MS
Sbjct: 179 SDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSA 238
Query: 182 VTTCLRFPGQLNADLRKLAVNMVPS 256
TT LR+PG +N DL + +++PS
Sbjct: 239 STTTLRYPGYMNNDLVSIIASLIPS 263
Score = 39.5 bits (88), Expect = 4e-04
Identities = 19/82 (23%), Positives = 44/82 (53%), Gaps = 4/82 (4%)
Frame = +1
Query: 256 PRLHFFMPGFAPLTSRGSRQYRAL---TVPELTQQMFDAKNMMAACDP-RHGRYLSVAAI 423
PR HF + + P T++ + +A+ TV ++ +++ KN M + +P + ++S+ I
Sbjct: 264 PRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNPSKKSCFISILDI 323
Query: 424 FRGRMSMKEVDEQMLNIQNKNF 489
+G +V + +L I+ + +
Sbjct: 324 IQGEADPADVHKSLLRIRERRY 345
>SPCC1742.01 ||SPCC1795.13, SPCPB16A4.07c|sequence
orphan|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1563
Score = 29.5 bits (63), Expect = 0.39
Identities = 12/33 (36%), Positives = 18/33 (54%)
Frame = +3
Query: 60 TQTKPTASTTRLYTTSASALSNCPHPPTVTLTT 158
T T +T + TTS S ++CP P T+ + T
Sbjct: 166 TSTSCNPATVLIVTTSGSTSTSCPPPTTILIVT 198
>SPBC27B12.05 |||WD repeat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 391
Score = 27.1 bits (57), Expect = 2.1
Identities = 14/47 (29%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +2
Query: 149 LNHLVSLTMSGVTTCLRFPGQLNADLR-KLAVNMVPSRVSTSSCQVS 286
L HLV + G++ C +PG+ N R K+ + + +V + Q+S
Sbjct: 331 LTHLVDAPLLGMSVCPLYPGEENPFKRFKIMLTVYTGKVISVEVQLS 377
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 26.6 bits (56), Expect = 2.7
Identities = 22/68 (32%), Positives = 32/68 (47%)
Frame = +2
Query: 47 QLVENTDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADL 226
Q+ NTD + N LY RT+ DLN SL + GV +F G + +
Sbjct: 301 QVCTNTDSSRNAGNAILYQ-AVRTIL-------DLNSDSSLRVLGVNILAKFLGNRDNNT 352
Query: 227 RKLAVNMV 250
R +A+NM+
Sbjct: 353 RYVALNML 360
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.8 bits (54), Expect = 4.8
Identities = 18/54 (33%), Positives = 22/54 (40%)
Frame = -2
Query: 339 LGYSQGTVLSASTGCEGSETWHEEVETREGTMFTANFLRSAFN*PGNLRQVVTP 178
L Y +SA+TG S WH TR T N R+ +LRQ P
Sbjct: 201 LSYLGAAGVSAATGAVNSIAWHPNNATRLATAIDDN--RNPIILTWDLRQPTVP 252
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 25.0 bits (52), Expect = 8.3
Identities = 8/30 (26%), Positives = 22/30 (73%)
Frame = +1
Query: 445 KEVDEQMLNIQNKNFLILRGMDPPTNVKDR 534
+++++Q+LN+ N+ F+I + P++++ R
Sbjct: 267 QKIEQQVLNLSNERFMIPELLFSPSDIEIR 296
>SPAC644.07 |||Rieske ISP assembly protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 449
Score = 25.0 bits (52), Expect = 8.3
Identities = 14/35 (40%), Positives = 16/35 (45%)
Frame = +2
Query: 62 TDETYCIDNEALYDICFRTLKLSTPTYGDLNHLVS 166
T Y + E YDIC L T LNHL+S
Sbjct: 256 TSFLYALAGELDYDICVLNLAEKGLTDDRLNHLLS 290
>SPAC23G3.06 |||U3 snoRNP protein Nop58 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 508
Score = 25.0 bits (52), Expect = 8.3
Identities = 10/23 (43%), Positives = 15/23 (65%)
Frame = -3
Query: 473 MLSICSSTSFMDMRPRKMAATLR 405
M + CS T F D+ P ++ ATL+
Sbjct: 214 MRTKCSETDFSDILPEEIEATLK 236
>SPAC1F8.05 |isp3|meu4|sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 182
Score = 25.0 bits (52), Expect = 8.3
Identities = 8/29 (27%), Positives = 12/29 (41%)
Frame = +3
Query: 222 ISENWP*TWFPPASPLLHARFRSPHIPWK 308
+ P W+PP P H + H W+
Sbjct: 38 VQHQQPTYWYPPPPPRHHKEHKKSHHHWE 66
>SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit
Rev3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1480
Score = 25.0 bits (52), Expect = 8.3
Identities = 10/23 (43%), Positives = 15/23 (65%), Gaps = 4/23 (17%)
Frame = -2
Query: 546 ISPHAVLHVCW----GIHSTKYE 490
+SP+A+L+ CW IH+ YE
Sbjct: 203 VSPNAILNACWLSERNIHTDLYE 225
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,598,620
Number of Sequences: 5004
Number of extensions: 52609
Number of successful extensions: 190
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 190
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 258201856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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