Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30309
(815 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 131 1e-31
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 127 2e-30
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 3.2
SPBC1861.01c |cnp3|SPBC56F2.13|CENP-C|Schizosaccharomyces pombe|... 27 4.2
SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces p... 27 4.2
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 26 5.6
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 26 5.6
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 26 5.6
SPCC1020.08 |||wybutosine biosynthesis protein Tyw1|Schizosaccha... 26 7.4
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 9.7
SPBC6B1.04 |mde4||monopolin-like complex subunit Mde4|Schizosacc... 25 9.7
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 131 bits (317), Expect = 1e-31
Identities = 79/172 (45%), Positives = 106/172 (61%), Gaps = 5/172 (2%)
Frame = +1
Query: 256 IEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMK 429
IEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVFY KMK
Sbjct: 68 IEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVFYYKMK 127
Query: 430 GDYYRYLAEVATGETRHSVVEDSQKASKMLLKSARRKCSPHTP*GWVWR*ISQSF-IMRY 606
GDYYRYLAE A GE R + S + K + A + +P P + ++ +F + Y
Sbjct: 128 GDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHP---IRLGLALNFSVFYY 184
Query: 607 *IRQT--RACQLAKQAFDDAIAELDT*TKIRTKILR**CSCCEINLTLWTSD 756
I + RAC LAKQAFD+AI+ELD+ ++ K NLTLWTSD
Sbjct: 185 EILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSD 236
Score = 82.2 bits (194), Expect = 8e-17
Identities = 39/69 (56%), Positives = 50/69 (72%)
Frame = +2
Query: 62 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSSW 241
+ +E+ V AKLAEQAERY+ M MK V T E + ERNLLSVAYKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 242 RVIS*LNRK 268
R++S + +K
Sbjct: 63 RIVSSIEQK 71
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 127 bits (307), Expect = 2e-30
Identities = 82/192 (42%), Positives = 101/192 (52%), Gaps = 6/192 (3%)
Frame = +1
Query: 256 IEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMK 429
IEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKVFY KMK
Sbjct: 67 IEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKVFYYKMK 126
Query: 430 GDYYRYLAEVATGETRHSVVEDSQKASKMLLKSARRKCSPHTP*GWVWR*ISQSFIMRY* 609
GDYYRYLAE GE + S +A K A + P P F
Sbjct: 127 GDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEIL 186
Query: 610 IRQTRACQLAKQAFDDAIAELDT*TKIRTKILR**CSCCEINLTLWTSDT----SGDGDE 777
AC LAKQ FD+AI+ELD+ ++ K NLTLWTSD S +
Sbjct: 187 DSPESACHLAKQVFDEAISELDSLSEESYKDSTLIMQLLRDNLTLWTSDAEYNQSAKEEA 246
Query: 778 LARGAATTNISP 813
A AA+ N P
Sbjct: 247 PAAAAASENEHP 258
Score = 81.0 bits (191), Expect = 2e-16
Identities = 40/70 (57%), Positives = 52/70 (74%)
Frame = +2
Query: 59 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSS 238
MS +E V AKLAEQAERY++M MK+V + + S ERNLLSVAYKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 239 WRVIS*LNRK 268
WR+IS + +K
Sbjct: 61 WRIISSIEQK 70
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 3.2
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +1
Query: 361 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 498
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC1861.01c |cnp3|SPBC56F2.13|CENP-C|Schizosaccharomyces pombe|chr
2|||Manual
Length = 643
Score = 26.6 bits (56), Expect = 4.2
Identities = 11/48 (22%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = -2
Query: 784 GQARRHRLKC-LTSKASS*SRSNCIINVESLYESSFKCPVRRWHRRKP 644
G+ +RH + + K++ ++SN + ++ Y+ C V W+ + P
Sbjct: 467 GRKKRHAKRSGVEIKSNLEAKSNDVEEYDAFYKDEINCEVLSWNEQNP 514
>SPBC3E7.11c |||DNAJ protein Caj1/Djp1-type|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 355
Score = 26.6 bits (56), Expect = 4.2
Identities = 21/69 (30%), Positives = 37/69 (53%), Gaps = 2/69 (2%)
Frame = +1
Query: 259 EQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM-KGD 435
E E+ Q++A+ Y+V + +LRE YD LG + +P A ++ F+ + GD
Sbjct: 43 ENPEAAREKFQKLAEAYQVLSDPKLRE-KYDKLGKVG--AVPDAGFEDAFEFFKNLFGGD 99
Query: 436 YYR-YLAEV 459
+R Y+ E+
Sbjct: 100 SFRDYVGEL 108
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.2 bits (55), Expect = 5.6
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +1
Query: 262 QKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 387
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 328 EKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/28 (39%), Positives = 14/28 (50%)
Frame = -3
Query: 681 SSVQFGDGIVESLFGELTRPCLANLISH 598
SS+ FG + S G CL N+ SH
Sbjct: 706 SSLHFGASQISSAIGSFAVGCLGNMYSH 733
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 26.2 bits (55), Expect = 5.6
Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 1/48 (2%)
Frame = +1
Query: 373 HLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQ-KASK 513
H SNP S K KG++ Y A G ++ +DS K SK
Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDSNVKCSK 272
>SPCC1020.08 |||wybutosine biosynthesis protein
Tyw1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 688
Score = 25.8 bits (54), Expect = 7.4
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = +1
Query: 247 HFLIEQKTEGSERKQQMAKEYRVKVEKELR 336
HFLI +KT+ S + K+ VK ++E++
Sbjct: 30 HFLIRRKTQLSNSDKSSEKKDEVKKQREVK 59
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 9.7
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +3
Query: 6 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 128
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPBC6B1.04 |mde4||monopolin-like complex subunit
Mde4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 421
Score = 25.4 bits (53), Expect = 9.7
Identities = 16/43 (37%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +1
Query: 280 ERKQQMAKEYRVKVEKELREICYDV--LGLLDKHLIPKASNPE 402
E++Q A +YR+KVE+ +I V + L+ L + SNPE
Sbjct: 85 EQEQNEANDYRLKVERLEHKISDYVQEINSLNSQLQIQKSNPE 127
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,248,323
Number of Sequences: 5004
Number of extensions: 63714
Number of successful extensions: 215
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 205
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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