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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30309
         (815 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)             123   2e-28
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)                      98   7e-21
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)             96   3e-20
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)              86   3e-17
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)              68   1e-11
SB_32481| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.0  
SB_10621| Best HMM Match : Peptidase_M24 (HMM E-Value=4.3e-14)         29   4.5  
SB_3265| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   6.0  
SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   6.0  
SB_5537| Best HMM Match : No HMM Matches (HMM E-Value=.)               29   6.0  
SB_30638| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.9  
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)                     28   7.9  

>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 309

 Score =  123 bits (297), Expect = 2e-28
 Identities = 73/146 (50%), Positives = 92/146 (63%), Gaps = 5/146 (3%)
 Frame = +1

Query: 256 IEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGD 435
           IEQK EGSERK+Q  + YR  +E EL E+C  VL LL+  LIP A + ESKVFYLKMKGD
Sbjct: 44  IEQKLEGSERKKQNTETYRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGD 103

Query: 436 YYRYLAEVATGETRHSVVEDSQKASKMLLKSARR--KCSPHTP*GWVWR*ISQSF-IMRY 606
           YYRY  EVA  + R  VV+ + KA     + A +  K  P  P   +   ++ +F +  Y
Sbjct: 104 YYRYEGEVAGADRRREVVQKAMKAYSEAQEIAEKDPKLPPTDP---IRLGLALNFSVFYY 160

Query: 607 *IRQ--TRACQLAKQAFDDAIAELDT 678
            I +   +AC LAK+AFDDAIAELDT
Sbjct: 161 EIVEDSKQACDLAKKAFDDAIAELDT 186



 Score = 70.9 bits (166), Expect = 1e-12
 Identities = 35/70 (50%), Positives = 43/70 (61%)
 Frame = +3

Query: 510 QDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKGVSTRQTGFRRCHRRTGHLNED 689
           ++A E    K+ PT PIRLGLALNFSVF+YEI  + ++     +  F         LNED
Sbjct: 220 KEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAFDDAIAELDSLNED 279

Query: 690 SYKDSTLIMQ 719
            YKDSTLIMQ
Sbjct: 280 QYKDSTLIMQ 289



 Score = 67.3 bits (157), Expect = 1e-11
 Identities = 36/75 (48%), Positives = 46/75 (61%), Gaps = 2/75 (2%)
 Frame = +3

Query: 501 ESIQDAFEISKA--KMQPTHPIRLGLALNFSVFYYEILNSPDKGVSTRQTGFRRCHRRTG 674
           ++  +A EI++   K+ PT PIRLGLALNFSVFYYEI+    +     +  F        
Sbjct: 126 KAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAELD 185

Query: 675 HLNEDSYKDSTLIMQ 719
            L+ED YKDSTLIMQ
Sbjct: 186 TLSEDQYKDSTLIMQ 200



 Score = 64.5 bits (150), Expect = 1e-10
 Identities = 32/47 (68%), Positives = 35/47 (74%)
 Frame = +2

Query: 128 MAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSSWRVIS*LNRK 268
           MA AMKE TE        ERNLLSVAYKNVVGA+RSSWRVIS + +K
Sbjct: 1   MAKAMKEATEISETLEQEERNLLSVAYKNVVGAKRSSWRVISSIEQK 47



 Score = 47.6 bits (108), Expect = 1e-05
 Identities = 27/55 (49%), Positives = 34/55 (61%), Gaps = 1/55 (1%)
 Frame = +1

Query: 616 QTRACQLAKQAFDDAIAELDT*TKIRTKILR**CSCCEINLTLWTSDTSGD-GDE 777
           Q  AC+LAKQAFDDAIAELD+  + + K           NLTLW+S+   D GD+
Sbjct: 255 QEEACKLAKQAFDDAIAELDSLNEDQYKDSTLIMQLLRDNLTLWSSENQEDQGDD 309


>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
          Length = 248

 Score = 98.3 bits (234), Expect = 7e-21
 Identities = 66/177 (37%), Positives = 90/177 (50%), Gaps = 3/177 (1%)
 Frame = +1

Query: 256 IEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKAS---NPESKVFYLKM 426
           +EQK    E    + K+YR  +  EL   C +VL +L+ +L+       N E+KVFYLKM
Sbjct: 73  MEQKAP--EEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDINTEAKVFYLKM 130

Query: 427 KGDYYRYLAEVATGETRHSVVEDSQKASKMLLKSARRKCSPHTP*GWVWR*ISQSFIMRY 606
           +GDY+RYL EVA G++R   +E S++A K     A  + SP  P           F    
Sbjct: 131 RGDYHRYLVEVAEGDSRKENIEKSREAYKDASAKA-EELSPSHPIRLGLALNFSVFYYEI 189

Query: 607 *IRQTRACQLAKQAFDDAIAELDT*TKIRTKILR**CSCCEINLTLWTSDTSGDGDE 777
             +   AC+LAK+AFDDAIA LD       K           NLTLWTS+   +G +
Sbjct: 190 ENKPPEACKLAKEAFDDAIAVLDNLKDESYKDSTLIMQLLRDNLTLWTSEQDQEGQD 246



 Score = 94.3 bits (224), Expect = 1e-19
 Identities = 46/74 (62%), Positives = 55/74 (74%)
 Frame = +2

Query: 47  PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGA 226
           P+      +EEL+  AK+AEQAERYDDM  AM  VT+ G   ++ ERNLLSVAYKNVVGA
Sbjct: 3   PNFVSKCSREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVAYKNVVGA 62

Query: 227 RRSSWRVIS*LNRK 268
           RRSSWRVIS + +K
Sbjct: 63  RRSSWRVISSMEQK 76



 Score = 72.1 bits (169), Expect = 5e-13
 Identities = 37/76 (48%), Positives = 49/76 (64%), Gaps = 3/76 (3%)
 Frame = +3

Query: 501 ESIQDAFEISKAK---MQPTHPIRLGLALNFSVFYYEILNSPDKGVSTRQTGFRRCHRRT 671
           E  ++A++ + AK   + P+HPIRLGLALNFSVFYYEI N P +     +  F       
Sbjct: 152 EKSREAYKDASAKAEELSPSHPIRLGLALNFSVFYYEIENKPPEACKLAKEAFDDAIAVL 211

Query: 672 GHLNEDSYKDSTLIMQ 719
            +L ++SYKDSTLIMQ
Sbjct: 212 DNLKDESYKDSTLIMQ 227


>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
          Length = 251

 Score = 96.3 bits (229), Expect = 3e-20
 Identities = 46/74 (62%), Positives = 57/74 (77%)
 Frame = +2

Query: 68  DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSSWRV 247
           DKEE V  AKLAEQAERYDDM  +MKEV + G E S  +RNLLSVAYKNV+GARR+SWR+
Sbjct: 3   DKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVAYKNVIGARRASWRI 62

Query: 248 IS*LNRKPRVQKEN 289
           I+ + +K   + E+
Sbjct: 63  ITSIEQKEESKGED 76



 Score = 51.6 bits (118), Expect = 7e-07
 Identities = 27/60 (45%), Positives = 37/60 (61%), Gaps = 3/60 (5%)
 Frame = +1

Query: 256 IEQKTEGSER---KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM 426
           IEQK E       K +M + YR  +E+EL+ IC ++L LLD  LI  + + ESKVFY K+
Sbjct: 66  IEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEESKVFYNKI 125


>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 219

 Score = 86.2 bits (204), Expect = 3e-17
 Identities = 42/70 (60%), Positives = 52/70 (74%)
 Frame = +2

Query: 59  MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSS 238
           M V +E L+  AKL+EQ +RYD+MA  MKEV+E   + S  ERNLLSV+YKN+VG RRSS
Sbjct: 80  MMVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVSYKNIVGQRRSS 139

Query: 239 WRVIS*LNRK 268
           WRVIS +  K
Sbjct: 140 WRVISSIEEK 149



 Score = 78.6 bits (185), Expect = 6e-15
 Identities = 37/72 (51%), Positives = 54/72 (75%)
 Frame = +1

Query: 256 IEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGD 435
           IE+KT  S     + K+Y+  +EKEL+++C +VLG+L++ LIP A + E+KVFY K+KGD
Sbjct: 146 IEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEENKVFYFKLKGD 203

Query: 436 YYRYLAEVATGE 471
           YYRYLAE + G+
Sbjct: 204 YYRYLAEFSHGQ 215


>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 607

 Score = 67.7 bits (158), Expect = 1e-11
 Identities = 32/62 (51%), Positives = 41/62 (66%)
 Frame = +2

Query: 59  MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSS 238
           M   + ELVQ AKLAEQ ER++D+   MK+  E     +   RNLLSV YKNVVG++R +
Sbjct: 186 MQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSVGYKNVVGSKRFA 245

Query: 239 WR 244
           WR
Sbjct: 246 WR 247


>SB_32481| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 499

 Score = 30.3 bits (65), Expect = 2.0
 Identities = 16/43 (37%), Positives = 26/43 (60%)
 Frame = +3

Query: 486 CRGFTESIQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNS 614
           C+G TE++Q+AFE S+     T P ++G A    ++ Y+  NS
Sbjct: 212 CKGKTENLQNAFEGSE-----TDPAKIGFAFYIGLWAYDGWNS 249


>SB_10621| Best HMM Match : Peptidase_M24 (HMM E-Value=4.3e-14)
          Length = 708

 Score = 29.1 bits (62), Expect = 4.5
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +2

Query: 116 RYDDMAAAMKEVTETGVEXSNXERNLLSV 202
           +YD++ AA+K+ T TG+  S  +  L  V
Sbjct: 57  KYDNLLAAVKDATNTGIRESGIDARLCDV 85



 Score = 29.1 bits (62), Expect = 4.5
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +2

Query: 116 RYDDMAAAMKEVTETGVEXSNXERNLLSV 202
           +YD++ AA+K+ T TG+  S  +  L  V
Sbjct: 163 KYDNLLAAVKDATNTGIRESGIDARLCDV 191


>SB_3265| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 118

 Score = 28.7 bits (61), Expect = 6.0
 Identities = 12/32 (37%), Positives = 17/32 (53%)
 Frame = +3

Query: 468 RNQTFCCRGFTESIQDAFEISKAKMQPTHPIR 563
           +N+T CC G T S ++ F + K K     P R
Sbjct: 10  KNKTICCPGRTFSYKEVFSMGKRKSASLSPQR 41


>SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 994

 Score = 28.7 bits (61), Expect = 6.0
 Identities = 15/51 (29%), Positives = 27/51 (52%)
 Frame = +2

Query: 35  ISPLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXER 187
           +SPLPS+      +E+V   KLAE+ E  ++++      T+   E +  E+
Sbjct: 643 VSPLPSTATEDQMQEVVDSNKLAEKKEVTEEVSPVKPLSTKESKENALEEK 693


>SB_5537| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 496

 Score = 28.7 bits (61), Expect = 6.0
 Identities = 21/74 (28%), Positives = 31/74 (41%), Gaps = 1/74 (1%)
 Frame = +1

Query: 334 REICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGET-RHSVVEDSQKAS 510
           R +    L    +HL+P+A +  S+ F  +      R+L   A   T RH V   S   S
Sbjct: 393 RHLVPRALDSTSRHLVPRALDSTSRHFVPQALDSTSRHLVPRALDSTSRHLVPRASDSTS 452

Query: 511 KMLLKSARRKCSPH 552
           + L+  A    S H
Sbjct: 453 RHLVPQALDSTSRH 466


>SB_30638| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 318

 Score = 28.3 bits (60), Expect = 7.9
 Identities = 15/47 (31%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
 Frame = +1

Query: 196 FSCL*ECRRCPTVIMACHFLIEQKTEGSERKQQMA--KEYRVKVEKE 330
           FSC  EC R   +  + +F IE+ ++G    + +   KE + K+EK+
Sbjct: 46  FSCTRECSRAEGLCASVNFYIEKGSDGKHLCELLTDDKEQQGKLEKD 92


>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
          Length = 783

 Score = 28.3 bits (60), Expect = 7.9
 Identities = 11/34 (32%), Positives = 22/34 (64%)
 Frame = +1

Query: 286 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 387
           K++M ++Y  K+EKE+    Y+++  L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 25,066,802
Number of Sequences: 59808
Number of extensions: 506465
Number of successful extensions: 1408
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1309
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1403
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2275631710
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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