BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30309 (815 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) 123 2e-28 SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) 98 7e-21 SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) 96 3e-20 SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) 86 3e-17 SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) 68 1e-11 SB_32481| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.0 SB_10621| Best HMM Match : Peptidase_M24 (HMM E-Value=4.3e-14) 29 4.5 SB_3265| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 6.0 SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 6.0 SB_5537| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 6.0 SB_30638| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.9 SB_12646| Best HMM Match : SSrecog (HMM E-Value=0) 28 7.9 >SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 309 Score = 123 bits (297), Expect = 2e-28 Identities = 73/146 (50%), Positives = 92/146 (63%), Gaps = 5/146 (3%) Frame = +1 Query: 256 IEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGD 435 IEQK EGSERK+Q + YR +E EL E+C VL LL+ LIP A + ESKVFYLKMKGD Sbjct: 44 IEQKLEGSERKKQNTETYRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGD 103 Query: 436 YYRYLAEVATGETRHSVVEDSQKASKMLLKSARR--KCSPHTP*GWVWR*ISQSF-IMRY 606 YYRY EVA + R VV+ + KA + A + K P P + ++ +F + Y Sbjct: 104 YYRYEGEVAGADRRREVVQKAMKAYSEAQEIAEKDPKLPPTDP---IRLGLALNFSVFYY 160 Query: 607 *IRQ--TRACQLAKQAFDDAIAELDT 678 I + +AC LAK+AFDDAIAELDT Sbjct: 161 EIVEDSKQACDLAKKAFDDAIAELDT 186 Score = 70.9 bits (166), Expect = 1e-12 Identities = 35/70 (50%), Positives = 43/70 (61%) Frame = +3 Query: 510 QDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKGVSTRQTGFRRCHRRTGHLNED 689 ++A E K+ PT PIRLGLALNFSVF+YEI + ++ + F LNED Sbjct: 220 KEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAFDDAIAELDSLNED 279 Query: 690 SYKDSTLIMQ 719 YKDSTLIMQ Sbjct: 280 QYKDSTLIMQ 289 Score = 67.3 bits (157), Expect = 1e-11 Identities = 36/75 (48%), Positives = 46/75 (61%), Gaps = 2/75 (2%) Frame = +3 Query: 501 ESIQDAFEISKA--KMQPTHPIRLGLALNFSVFYYEILNSPDKGVSTRQTGFRRCHRRTG 674 ++ +A EI++ K+ PT PIRLGLALNFSVFYYEI+ + + F Sbjct: 126 KAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDAIAELD 185 Query: 675 HLNEDSYKDSTLIMQ 719 L+ED YKDSTLIMQ Sbjct: 186 TLSEDQYKDSTLIMQ 200 Score = 64.5 bits (150), Expect = 1e-10 Identities = 32/47 (68%), Positives = 35/47 (74%) Frame = +2 Query: 128 MAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSSWRVIS*LNRK 268 MA AMKE TE ERNLLSVAYKNVVGA+RSSWRVIS + +K Sbjct: 1 MAKAMKEATEISETLEQEERNLLSVAYKNVVGAKRSSWRVISSIEQK 47 Score = 47.6 bits (108), Expect = 1e-05 Identities = 27/55 (49%), Positives = 34/55 (61%), Gaps = 1/55 (1%) Frame = +1 Query: 616 QTRACQLAKQAFDDAIAELDT*TKIRTKILR**CSCCEINLTLWTSDTSGD-GDE 777 Q AC+LAKQAFDDAIAELD+ + + K NLTLW+S+ D GD+ Sbjct: 255 QEEACKLAKQAFDDAIAELDSLNEDQYKDSTLIMQLLRDNLTLWSSENQEDQGDD 309 >SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0) Length = 248 Score = 98.3 bits (234), Expect = 7e-21 Identities = 66/177 (37%), Positives = 90/177 (50%), Gaps = 3/177 (1%) Frame = +1 Query: 256 IEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKAS---NPESKVFYLKM 426 +EQK E + K+YR + EL C +VL +L+ +L+ N E+KVFYLKM Sbjct: 73 MEQKAP--EEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDINTEAKVFYLKM 130 Query: 427 KGDYYRYLAEVATGETRHSVVEDSQKASKMLLKSARRKCSPHTP*GWVWR*ISQSFIMRY 606 +GDY+RYL EVA G++R +E S++A K A + SP P F Sbjct: 131 RGDYHRYLVEVAEGDSRKENIEKSREAYKDASAKA-EELSPSHPIRLGLALNFSVFYYEI 189 Query: 607 *IRQTRACQLAKQAFDDAIAELDT*TKIRTKILR**CSCCEINLTLWTSDTSGDGDE 777 + AC+LAK+AFDDAIA LD K NLTLWTS+ +G + Sbjct: 190 ENKPPEACKLAKEAFDDAIAVLDNLKDESYKDSTLIMQLLRDNLTLWTSEQDQEGQD 246 Score = 94.3 bits (224), Expect = 1e-19 Identities = 46/74 (62%), Positives = 55/74 (74%) Frame = +2 Query: 47 PSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGA 226 P+ +EEL+ AK+AEQAERYDDM AM VT+ G ++ ERNLLSVAYKNVVGA Sbjct: 3 PNFVSKCSREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVAYKNVVGA 62 Query: 227 RRSSWRVIS*LNRK 268 RRSSWRVIS + +K Sbjct: 63 RRSSWRVISSMEQK 76 Score = 72.1 bits (169), Expect = 5e-13 Identities = 37/76 (48%), Positives = 49/76 (64%), Gaps = 3/76 (3%) Frame = +3 Query: 501 ESIQDAFEISKAK---MQPTHPIRLGLALNFSVFYYEILNSPDKGVSTRQTGFRRCHRRT 671 E ++A++ + AK + P+HPIRLGLALNFSVFYYEI N P + + F Sbjct: 152 EKSREAYKDASAKAEELSPSHPIRLGLALNFSVFYYEIENKPPEACKLAKEAFDDAIAVL 211 Query: 672 GHLNEDSYKDSTLIMQ 719 +L ++SYKDSTLIMQ Sbjct: 212 DNLKDESYKDSTLIMQ 227 >SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44) Length = 251 Score = 96.3 bits (229), Expect = 3e-20 Identities = 46/74 (62%), Positives = 57/74 (77%) Frame = +2 Query: 68 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSSWRV 247 DKEE V AKLAEQAERYDDM +MKEV + G E S +RNLLSVAYKNV+GARR+SWR+ Sbjct: 3 DKEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVAYKNVIGARRASWRI 62 Query: 248 IS*LNRKPRVQKEN 289 I+ + +K + E+ Sbjct: 63 ITSIEQKEESKGED 76 Score = 51.6 bits (118), Expect = 7e-07 Identities = 27/60 (45%), Positives = 37/60 (61%), Gaps = 3/60 (5%) Frame = +1 Query: 256 IEQKTEGSER---KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKM 426 IEQK E K +M + YR +E+EL+ IC ++L LLD LI + + ESKVFY K+ Sbjct: 66 IEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEESKVFYNKI 125 >SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 219 Score = 86.2 bits (204), Expect = 3e-17 Identities = 42/70 (60%), Positives = 52/70 (74%) Frame = +2 Query: 59 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSS 238 M V +E L+ AKL+EQ +RYD+MA MKEV+E + S ERNLLSV+YKN+VG RRSS Sbjct: 80 MMVVRETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVSYKNIVGQRRSS 139 Query: 239 WRVIS*LNRK 268 WRVIS + K Sbjct: 140 WRVISSIEEK 149 Score = 78.6 bits (185), Expect = 6e-15 Identities = 37/72 (51%), Positives = 54/72 (75%) Frame = +1 Query: 256 IEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGD 435 IE+KT S + K+Y+ +EKEL+++C +VLG+L++ LIP A + E+KVFY K+KGD Sbjct: 146 IEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEENKVFYFKLKGD 203 Query: 436 YYRYLAEVATGE 471 YYRYLAE + G+ Sbjct: 204 YYRYLAEFSHGQ 215 >SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 607 Score = 67.7 bits (158), Expect = 1e-11 Identities = 32/62 (51%), Positives = 41/62 (66%) Frame = +2 Query: 59 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXERNLLSVAYKNVVGARRSS 238 M + ELVQ AKLAEQ ER++D+ MK+ E + RNLLSV YKNVVG++R + Sbjct: 186 MQDSRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSVGYKNVVGSKRFA 245 Query: 239 WR 244 WR Sbjct: 246 WR 247 >SB_32481| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 499 Score = 30.3 bits (65), Expect = 2.0 Identities = 16/43 (37%), Positives = 26/43 (60%) Frame = +3 Query: 486 CRGFTESIQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNS 614 C+G TE++Q+AFE S+ T P ++G A ++ Y+ NS Sbjct: 212 CKGKTENLQNAFEGSE-----TDPAKIGFAFYIGLWAYDGWNS 249 >SB_10621| Best HMM Match : Peptidase_M24 (HMM E-Value=4.3e-14) Length = 708 Score = 29.1 bits (62), Expect = 4.5 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = +2 Query: 116 RYDDMAAAMKEVTETGVEXSNXERNLLSV 202 +YD++ AA+K+ T TG+ S + L V Sbjct: 57 KYDNLLAAVKDATNTGIRESGIDARLCDV 85 Score = 29.1 bits (62), Expect = 4.5 Identities = 11/29 (37%), Positives = 18/29 (62%) Frame = +2 Query: 116 RYDDMAAAMKEVTETGVEXSNXERNLLSV 202 +YD++ AA+K+ T TG+ S + L V Sbjct: 163 KYDNLLAAVKDATNTGIRESGIDARLCDV 191 >SB_3265| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 118 Score = 28.7 bits (61), Expect = 6.0 Identities = 12/32 (37%), Positives = 17/32 (53%) Frame = +3 Query: 468 RNQTFCCRGFTESIQDAFEISKAKMQPTHPIR 563 +N+T CC G T S ++ F + K K P R Sbjct: 10 KNKTICCPGRTFSYKEVFSMGKRKSASLSPQR 41 >SB_42896| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 994 Score = 28.7 bits (61), Expect = 6.0 Identities = 15/51 (29%), Positives = 27/51 (52%) Frame = +2 Query: 35 ISPLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVEXSNXER 187 +SPLPS+ +E+V KLAE+ E ++++ T+ E + E+ Sbjct: 643 VSPLPSTATEDQMQEVVDSNKLAEKKEVTEEVSPVKPLSTKESKENALEEK 693 >SB_5537| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 496 Score = 28.7 bits (61), Expect = 6.0 Identities = 21/74 (28%), Positives = 31/74 (41%), Gaps = 1/74 (1%) Frame = +1 Query: 334 REICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGET-RHSVVEDSQKAS 510 R + L +HL+P+A + S+ F + R+L A T RH V S S Sbjct: 393 RHLVPRALDSTSRHLVPRALDSTSRHFVPQALDSTSRHLVPRALDSTSRHLVPRASDSTS 452 Query: 511 KMLLKSARRKCSPH 552 + L+ A S H Sbjct: 453 RHLVPQALDSTSRH 466 >SB_30638| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 318 Score = 28.3 bits (60), Expect = 7.9 Identities = 15/47 (31%), Positives = 26/47 (55%), Gaps = 2/47 (4%) Frame = +1 Query: 196 FSCL*ECRRCPTVIMACHFLIEQKTEGSERKQQMA--KEYRVKVEKE 330 FSC EC R + + +F IE+ ++G + + KE + K+EK+ Sbjct: 46 FSCTRECSRAEGLCASVNFYIEKGSDGKHLCELLTDDKEQQGKLEKD 92 >SB_12646| Best HMM Match : SSrecog (HMM E-Value=0) Length = 783 Score = 28.3 bits (60), Expect = 7.9 Identities = 11/34 (32%), Positives = 22/34 (64%) Frame = +1 Query: 286 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 387 K++M ++Y K+EKE+ Y+++ L K ++ K Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 25,066,802 Number of Sequences: 59808 Number of extensions: 506465 Number of successful extensions: 1408 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 1309 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1403 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2275631710 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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