Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30306
(334 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 105 1e-24
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 56 1e-09
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 56 1e-09
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 56 1e-09
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 36 0.002
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 0.75
SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr 1||... 26 1.3
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 25 3.0
SPAC24H6.04 |hxk1||hexokinase 1 |Schizosaccharomyces pombe|chr 1... 25 3.0
SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces pom... 25 3.0
SPAC806.02c |||Par A family ATPase iron cluster assembly protein... 25 4.0
SPAC1527.02 |sft2||Golgi transport protein Sft2 |Schizosaccharom... 25 4.0
SPAC22G7.07c |||mRNA |Schizosaccharomyces pombe|chr 1|||Manual 24 5.3
SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyce... 24 7.0
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 23 9.2
SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3 |Sc... 23 9.2
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 105 bits (253), Expect = 1e-24
Identities = 50/87 (57%), Positives = 64/87 (73%)
Frame = +3
Query: 3 LLKEVDTFIPTPIRELEKPFLMPVESVHSIPGRGTVITGRLYRGVLKKGTDCEIVGHGKT 182
L++ VD++I P R+ + PFLM +E V SI GRGTV+TGR+ RG LKKG + EIVG+G
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKGAEIEIVGYGSH 293
Query: 183 MKTTVTGVEMFHKTLEEAQAGDQLELL 263
+KTTVTG+EMF K L+ A AGD LL
Sbjct: 294 LKTTVTGIEMFKKQLDAAVAGDNCGLL 320
Score = 36.3 bits (80), Expect = 0.001
Identities = 14/25 (56%), Positives = 20/25 (80%)
Frame = +2
Query: 260 LVRSIKRGQIKRGIVMGEPGTAKAH 334
L+RSIKR Q+KRG+++ +PGT H
Sbjct: 320 LLRSIKREQLKRGMIVAQPGTVAPH 344
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 56.0 bits (129), Expect = 1e-09
Identities = 32/84 (38%), Positives = 46/84 (54%)
Frame = +3
Query: 3 LLKEVDTFIPTPIRELEKPFLMPVESVHSIPGRGTVITGRLYRGVLKKGTDCEIVGHGKT 182
LL+ +D+ P P R +KP +P++ V+ I G GTV GR+ GV+K G G T
Sbjct: 226 LLEAIDSIEP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVT 284
Query: 183 MKTTVTGVEMFHKTLEEAQAGDQL 254
T V VEM H++L+ GD +
Sbjct: 285 --TEVKSVEMHHESLDAGLPGDNV 306
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 56.0 bits (129), Expect = 1e-09
Identities = 32/84 (38%), Positives = 46/84 (54%)
Frame = +3
Query: 3 LLKEVDTFIPTPIRELEKPFLMPVESVHSIPGRGTVITGRLYRGVLKKGTDCEIVGHGKT 182
LL+ +D+ P P R +KP +P++ V+ I G GTV GR+ GV+K G G T
Sbjct: 226 LLEAIDSIEP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVT 284
Query: 183 MKTTVTGVEMFHKTLEEAQAGDQL 254
T V VEM H++L+ GD +
Sbjct: 285 --TEVKSVEMHHESLDAGLPGDNV 306
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 56.0 bits (129), Expect = 1e-09
Identities = 32/84 (38%), Positives = 46/84 (54%)
Frame = +3
Query: 3 LLKEVDTFIPTPIRELEKPFLMPVESVHSIPGRGTVITGRLYRGVLKKGTDCEIVGHGKT 182
LL+ +D+ P P R +KP +P++ V+ I G GTV GR+ GV+K G G T
Sbjct: 226 LLEAIDSIEP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMIVTFAPAGVT 284
Query: 183 MKTTVTGVEMFHKTLEEAQAGDQL 254
T V VEM H++L+ GD +
Sbjct: 285 --TEVKSVEMHHESLDAGLPGDNV 306
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 35.9 bits (79), Expect = 0.002
Identities = 19/73 (26%), Positives = 40/73 (54%)
Frame = +3
Query: 42 RELEKPFLMPVESVHSIPGRGTVITGRLYRGVLKKGTDCEIVGHGKTMKTTVTGVEMFHK 221
R++ PF+MP+ S + GT++ G++ G +KK ++ ++ +T++ T E +
Sbjct: 463 RKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPINQTLEVTAIYDEA-DE 519
Query: 222 TLEEAQAGDQLEL 260
+ + GDQ+ L
Sbjct: 520 EISSSICGDQVRL 532
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 27.1 bits (57), Expect = 0.75
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -1
Query: 208 STPVTVVFIVLP*PTISQSVPFFKTPLYKRPVMT 107
STPVT + T S SVP+ TP+ P+ T
Sbjct: 437 STPVTSTPLATTNCTTSTSVPYTSTPVTSTPLTT 470
Score = 26.6 bits (56), Expect = 0.99
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -1
Query: 208 STPVTVVFIVLP*PTISQSVPFFKTPLYKRPVMT 107
STPVT + T S S+P+ TP+ P+ T
Sbjct: 460 STPVTSTPLTTTNCTTSTSIPYTSTPVTSTPLTT 493
Score = 23.4 bits (48), Expect = 9.2
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -1
Query: 208 STPVTVVFIVLP*PTISQSVPFFKTPL 128
STPVT + T S SVP+ TP+
Sbjct: 483 STPVTSTPLTTTNCTTSTSVPYTSTPV 509
Score = 23.4 bits (48), Expect = 9.2
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -1
Query: 208 STPVTVVFIVLP*PTISQSVPFFKTPLYKRPVMT 107
STPVT + T S SV + TP+ P+ T
Sbjct: 519 STPVTSTPVTTTNCTTSTSVLYTSTPVTSTPLAT 552
Score = 23.4 bits (48), Expect = 9.2
Identities = 12/27 (44%), Positives = 15/27 (55%)
Frame = -1
Query: 208 STPVTVVFIVLP*PTISQSVPFFKTPL 128
STPVT + T S SVP+ TP+
Sbjct: 542 STPVTSTPLATTNCTTSTSVPYTSTPV 568
>SPAC823.09c |||L-asparaginase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 285
Score = 26.2 bits (55), Expect = 1.3
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +3
Query: 177 KTMKTTVTGVEMFHKTLEEAQAGDQLELLCVPSKGDKSNAVLSWANRVLLKP 332
++MKT + E+ ++ + D + ++CV G AV+S + +LLKP
Sbjct: 152 RSMKTQIKWKEILYQNPINLSSQDTIGVICVDKNG--RIAVVSSSGGLLLKP 201
>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 983
Score = 25.0 bits (52), Expect = 3.0
Identities = 12/50 (24%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +3
Query: 42 RELEKPFLMPVESVHS-IPGRGTVITGRLYRGVLKKGTDCEIVGHGKTMK 188
RE P +M V +++ + R+Y G +KKG +++G +++
Sbjct: 481 REESAPLVMHVTKLYNTVDANNFYAFARVYSGQVKKGQKVKVLGENYSLE 530
>SPAC24H6.04 |hxk1||hexokinase 1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 484
Score = 25.0 bits (52), Expect = 3.0
Identities = 13/41 (31%), Positives = 20/41 (48%)
Frame = +3
Query: 45 ELEKPFLMPVESVHSIPGRGTVITGRLYRGVLKKGTDCEIV 167
ELE+ F +P E +H + R LY+G+ D +V
Sbjct: 33 ELEEQFTIPTELLHRVTDR---FVSELYKGLTTNPGDVPMV 70
>SPAC227.01c ||SPAPB21F2.04c|Erd1 homolog|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 373
Score = 25.0 bits (52), Expect = 3.0
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -1
Query: 220 LWNISTPVTVVFIVLP*PTISQSVPFFKTPL 128
LWN S+ + + ++ S+PFFK PL
Sbjct: 248 LWNTSSAINSTYSFWWDVSMDWSLPFFKQPL 278
>SPAC806.02c |||Par A family ATPase iron cluster assembly
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 608
Score = 24.6 bits (51), Expect = 4.0
Identities = 12/23 (52%), Positives = 14/23 (60%)
Frame = -3
Query: 224 GFVEHFNSGYGCFHCFTMTNNFT 156
G VE+ SGY C HC TN F+
Sbjct: 191 GIVENM-SGYICPHCADCTNIFS 212
>SPAC1527.02 |sft2||Golgi transport protein Sft2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 201
Score = 24.6 bits (51), Expect = 4.0
Identities = 9/22 (40%), Positives = 11/22 (50%)
Frame = -3
Query: 272 WNAQELKLISGLCFLQGFVEHF 207
W L + G +QGFV HF
Sbjct: 103 WTMGSLLAVLGFAIVQGFVAHF 124
>SPAC22G7.07c |||mRNA |Schizosaccharomyces pombe|chr 1|||Manual
Length = 413
Score = 24.2 bits (50), Expect = 5.3
Identities = 11/30 (36%), Positives = 19/30 (63%), Gaps = 2/30 (6%)
Frame = +3
Query: 27 IPTPIRELEKPFLMPVESV--HSIPGRGTV 110
+PT + + KP+L+P +S + G+GTV
Sbjct: 21 LPTRTKPVVKPYLLPEKSTSDYENSGKGTV 50
>SPAC18G6.02c |chp1||chromodomain protein Chp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 960
Score = 23.8 bits (49), Expect = 7.0
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 27 IPTPIRELEKPFLMPVESVHSIPG 98
IP E++ LMP +S +S+PG
Sbjct: 213 IPLKNGEIKSTMLMPSDSDNSVPG 236
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 23.4 bits (48), Expect = 9.2
Identities = 16/42 (38%), Positives = 19/42 (45%)
Frame = +3
Query: 72 VESVHSIPGRGTVITGRLYRGVLKKGTDCEIVGHGKTMKTTV 197
V V I G G I L GVL +G + G G + TTV
Sbjct: 713 VLEVKVIEGLGATIDVILSNGVLHEGDRIVLCGMGGPIITTV 754
>SPCC1442.10c |rpb3||DNA-directed RNA polymerase II subunit 3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 297
Score = 23.4 bits (48), Expect = 9.2
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = +3
Query: 30 PTPIRELEKPFLMPVESVHSIPGRGTVITG 119
P +E + F M VESV SIP ++ G
Sbjct: 219 PFNFQEEPRRFYMDVESVGSIPPNEIMVQG 248
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,411,802
Number of Sequences: 5004
Number of extensions: 26240
Number of successful extensions: 97
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 88
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 94
length of database: 2,362,478
effective HSP length: 64
effective length of database: 2,042,222
effective search space used: 93942212
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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