Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30302
(658 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 158 8e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 64 1e-11
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 64 2e-11
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 59 6e-10
SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|ch... 28 1.0
SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr 3|||Ma... 28 1.0
SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb... 27 1.8
SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr 1|... 26 4.2
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 26 4.2
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 26 4.2
SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr ... 26 5.5
SPAC4G8.08 |||iron ion transporter |Schizosaccharomyces pombe|ch... 26 5.5
SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 25 7.3
SPCC1020.03 |||mitochondrial iron ion transporter|Schizosaccharo... 25 7.3
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.3
SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces pomb... 25 9.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 158 bits (383), Expect = 8e-40
Identities = 93/209 (44%), Positives = 116/209 (55%), Gaps = 6/209 (2%)
Frame = +2
Query: 2 FWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQ-----VRAPRHPRRLGARHHG 166
FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GG+ V P + A G
Sbjct: 20 FWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSG 79
Query: 167 LCPLWTFRTDLPSXXXXXXXXXXXXXXXXXHYTEGSELVDSVLDVVRKEAESCDCLQGFQ 346
FR D HYTEG+EL D+VLDVVR+EAE+CD LQGFQ
Sbjct: 80 KFGN-LFRPD----NIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQ 134
Query: 347 XXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSFIN*LK 526
KIREEYPDR+M T+SV P+PK SDTVVEPYNATLS ++
Sbjct: 135 LTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVE 194
Query: 527 TQTKP-TVSTTRLSTISAFRTLKLSTPTY 610
+ + LS+I A TLK+ +P+Y
Sbjct: 195 NSDETFCIDNEALSSIFA-NTLKIKSPSY 222
Score = 87.4 bits (207), Expect = 2e-18
Identities = 36/46 (78%), Positives = 42/46 (91%)
Frame = +3
Query: 117 KYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAK 254
KYVPRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAK
Sbjct: 58 KYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAK 103
Score = 56.4 bits (130), Expect = 3e-09
Identities = 29/50 (58%), Positives = 35/50 (70%)
Frame = +1
Query: 508 IHQLVENTDETYCIDNEALYDICFPHAKTIHTHIRATLNHLVSLTMSGVT 657
+HQLVEN+DET+CIDNEAL I F + I + LNHLVS M+GVT
Sbjct: 189 MHQLVENSDETFCIDNEALSSI-FANTLKIKSPSYDDLNHLVSAVMAGVT 237
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 64.5 bits (150), Expect = 1e-11
Identities = 31/84 (36%), Positives = 47/84 (55%)
Frame = +2
Query: 257 HYTEGSELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 436
HYT G E++DSVL+ +R+ A++C LQGF ++ EY +
Sbjct: 111 HYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSN 170
Query: 437 NTYSVVPSPKVSDTVVEPYNATLS 508
+SV P+P+VS +VVEPYN+ L+
Sbjct: 171 LQFSVYPAPQVSTSVVEPYNSVLT 194
Score = 58.0 bits (134), Expect = 1e-09
Identities = 23/46 (50%), Positives = 33/46 (71%)
Frame = +3
Query: 117 KYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAK 254
K+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+
Sbjct: 64 KFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYAR 109
Score = 37.5 bits (83), Expect = 0.002
Identities = 18/49 (36%), Positives = 28/49 (57%)
Frame = +1
Query: 511 HQLVENTDETYCIDNEALYDICFPHAKTIHTHIRATLNHLVSLTMSGVT 657
H ++N+D T+ +DNEA YDIC + I LN L++ +S +T
Sbjct: 196 HATLDNSDCTFMVDNEACYDICRRNL-DIERPTYENLNRLIAQVVSSIT 243
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 64.1 bits (149), Expect = 2e-11
Identities = 32/84 (38%), Positives = 45/84 (53%)
Frame = +2
Query: 257 HYTEGSELVDSVLDVVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIM 436
HYT G ELVD V D +R+ A++C LQGF ++ EY +
Sbjct: 107 HYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSK 166
Query: 437 NTYSVVPSPKVSDTVVEPYNATLS 508
+SV P+P+VS +VVEPYN+ L+
Sbjct: 167 LQFSVYPAPQVSTSVVEPYNSVLT 190
Score = 59.3 bits (137), Expect = 5e-10
Identities = 23/46 (50%), Positives = 33/46 (71%)
Frame = +3
Query: 117 KYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAK 254
KYVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+
Sbjct: 60 KYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYAR 105
Score = 31.9 bits (69), Expect = 0.084
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = +1
Query: 511 HQLVENTDETYCIDNEALYDICFPHAKTIHTHIRATLNHLVSLTMSGVT 657
H ++ D T+ +DNE+ YDIC + I LN L++ +S +T
Sbjct: 192 HATLDLADCTFMVDNESCYDICRRNL-DIERPSYENLNRLIAQVVSSIT 239
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.8 bits (136), Expect = 6e-10
Identities = 48/207 (23%), Positives = 86/207 (41%), Gaps = 4/207 (1%)
Frame = +2
Query: 2 FWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGQV--RAPRHPRRLGARHHGLCP 175
FW+ + EHGI P G + ++R +V++ ++ + RA ++ L
Sbjct: 21 FWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSD 80
Query: 176 LWTFRTDLPSXXXXXXXXXXXXXXXXXHYTEGSELVDSVLDVVRKEAESCDCLQGFQXXX 355
+ + P Y+ + + ++D++ +EA+ D L+GF
Sbjct: 81 TYGSLYN-PENILITKNGGGAGNNWANGYSHAERIFEDIMDMIDREADGSDSLEGFSLLH 139
Query: 356 XXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSFIN-*LKT 529
++ + YP +I+ TYSV P S VSD VV+PYN+ L+ L
Sbjct: 140 SIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNA 199
Query: 530 QTKPTVSTTRLSTISAFRTLKLSTPTY 610
+ + L+ I+A R L PT+
Sbjct: 200 DSVVVLDNAALAHIAADR-LHTQNPTF 225
Score = 54.4 bits (125), Expect = 1e-08
Identities = 21/47 (44%), Positives = 35/47 (74%), Gaps = 2/47 (4%)
Frame = +3
Query: 117 KYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWA 251
+Y+PRAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA
Sbjct: 59 RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWA 105
>SPAC27D7.11c |||But2 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 463
Score = 28.3 bits (60), Expect = 1.0
Identities = 15/54 (27%), Positives = 30/54 (55%)
Frame = +2
Query: 446 SVVPSPKVSDTVVEPYNATLSFIN*LKTQTKPTVSTTRLSTISAFRTLKLSTPT 607
+V+P P S + YN+T S + + PT+++T+ ST+++ ++T T
Sbjct: 164 AVLPQPISSSST---YNSTTSSYHNSTSTPPPTITSTKASTVTSTEATTVTTTT 214
>SPCC320.04c |||GTPase Gem1 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 630
Score = 28.3 bits (60), Expect = 1.0
Identities = 19/72 (26%), Positives = 35/72 (48%), Gaps = 3/72 (4%)
Frame = -1
Query: 358 RVCQLESLEAITRFRFL---SNDV*N*IDELRTLRVVSLAQLLPAPDCPKTKLSGRKICP 188
R + ++ A++R FL +ND +DEL +L ++ L D + ++ICP
Sbjct: 179 RTMRKATIHALSRIFFLIDKNNDDLLSVDELNSLSEKCFSKNLSIEDASEILSKVKEICP 238
Query: 187 KGPERTESMVPG 152
+G + +PG
Sbjct: 239 EGVYEGQLTLPG 250
>SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1328
Score = 27.5 bits (58), Expect = 1.8
Identities = 19/50 (38%), Positives = 25/50 (50%), Gaps = 9/50 (18%)
Frame = +3
Query: 198 FRPDNFVFGQSGAGNNWAK-------DTTRRV--LSSSIQF*TSFERKRN 320
F P+NF Q+G +W D TR + L+S +F T ERKRN
Sbjct: 607 FYPENFALDQNGKTASWEAVVIIPFIDETRLIDALASKDKFLTEEERKRN 656
>SPAC977.17 |||MIP water channel|Schizosaccharomyces pombe|chr
1|||Manual
Length = 598
Score = 26.2 bits (55), Expect = 4.2
Identities = 15/47 (31%), Positives = 22/47 (46%)
Frame = -3
Query: 311 PFERRLKLNRRAQNPPCSVLGPVVAGAGLSEDEVVRTEDLSERSRAD 171
P+ L RR P S+ GP+ G S E + ++ RSRA+
Sbjct: 103 PYYEELNAGRRPNKPVWSLNGPLPHVLGNSVVEKISQKNQEARSRAN 149
>SPAC328.05 |||RNA-binding protein involved in export of
mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -2
Query: 654 HAGHGQRHQVVQGRPYVGVDSFSVRKADIVE 562
H G+GQ++ + R YVG S+ VR ++ E
Sbjct: 54 HLGNGQKYTQQERRVYVGNLSYQVRWFELKE 84
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.2 bits (55), Expect = 4.2
Identities = 10/25 (40%), Positives = 15/25 (60%), Gaps = 1/25 (4%)
Frame = +3
Query: 180 GPFGQIFRPDNFVFGQSGAG-NNWA 251
GP+G +F P F+F +G NW+
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWS 181
>SPAC227.06 |||Rab GTPase binding |Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 25.8 bits (54), Expect = 5.5
Identities = 15/58 (25%), Positives = 30/58 (51%)
Frame = -2
Query: 636 RHQVVQGRPYVGVDSFSVRKADIVESLVVDTVGFVCVFN*LMNESVALYGSTTVSDTL 463
R +Q P+ DSF+V D+V+ + + V F+ ++++ LYG ++ T+
Sbjct: 41 RRASMQETPWTIKDSFNVETKDVVQRCIHTLIPTVNFFD-VVDDRPDLYGPFWITTTV 97
>SPAC4G8.08 |||iron ion transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 271
Score = 25.8 bits (54), Expect = 5.5
Identities = 16/45 (35%), Positives = 23/45 (51%)
Frame = -3
Query: 269 PPCSVLGPVVAGAGLSEDEVVRTEDLSERSRADRVHGAGLQVDED 135
P CS LG VVA + ++V+T S A R GLQ +++
Sbjct: 177 PICSALGTVVATTISTPLDIVKTRYQVATSSAMRKAEYGLQAEKE 221
>SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1036
Score = 25.4 bits (53), Expect = 7.3
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 7/63 (11%)
Frame = +2
Query: 437 NTYSVVPSPKVSDTVVEPYNATLSF------IN*LKTQTKPTVSTTRLSTI-SAFRTLKL 595
NT SV+P+ VS T + N+T + ++ + + T + S+T LS++ S T
Sbjct: 410 NTSSVLPTSSVSSTPLSSANSTTATSASSTPLSSVNSTTATSASSTPLSSVNSTTATSAS 469
Query: 596 STP 604
STP
Sbjct: 470 STP 472
>SPCC1020.03 |||mitochondrial iron ion
transporter|Schizosaccharomyces pombe|chr 3|||Manual
Length = 397
Score = 25.4 bits (53), Expect = 7.3
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = +1
Query: 562 LYDICFPHAKTIHTHI 609
LY I FP+A + HTHI
Sbjct: 169 LYTILFPYAGSEHTHI 184
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.3
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 238 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 143
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPBC56F2.01 |pof12||F-box protein Pof12|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 440
Score = 25.0 bits (52), Expect = 9.6
Identities = 14/51 (27%), Positives = 24/51 (47%)
Frame = +1
Query: 466 SVGHSSRTVQRHTFIHQLVENTDETYCIDNEALYDICFPHAKTIHTHIRAT 618
SVG+S + +F + V++ C+DN +LY I +H + T
Sbjct: 137 SVGNSLKCQAISSFRDETVKSGPAVMCLDNASLY-IGLKDGNLLHVTVHET 186
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,569,187
Number of Sequences: 5004
Number of extensions: 49058
Number of successful extensions: 161
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 155
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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