Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30300
(777 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 128 7e-31
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 128 1e-30
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 71 2e-13
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 58 1e-09
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 30 0.32
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 29 0.74
SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 27 3.0
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.9
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 9.2
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.2
SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|... 25 9.2
SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces pomb... 25 9.2
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 128 bits (310), Expect = 7e-31
Identities = 56/85 (65%), Positives = 67/85 (78%)
Frame = +3
Query: 255 PTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTG 434
P V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+G
Sbjct: 72 PNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSG 131
Query: 435 LQGFLIFHXXXXXXXXXXXXLLMER 509
LQGFL+FH LL+ER
Sbjct: 132 LQGFLVFHSFGGGTGSGFGALLLER 156
Score = 108 bits (260), Expect = 8e-25
Identities = 46/68 (67%), Positives = 54/68 (79%)
Frame = +1
Query: 43 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 222
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 223 HVPRAVFV 246
+VPR+++V
Sbjct: 61 YVPRSIYV 68
Score = 95.5 bits (227), Expect = 8e-21
Identities = 40/56 (71%), Positives = 51/56 (91%)
Frame = +2
Query: 509 LSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDI 676
L+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDI
Sbjct: 157 LAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDI 212
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 128 bits (308), Expect = 1e-30
Identities = 54/95 (56%), Positives = 71/95 (74%)
Frame = +3
Query: 255 PTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTG 434
P V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+G
Sbjct: 76 PNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSG 135
Query: 435 LQGFLIFHXXXXXXXXXXXXLLMERSPLTTARSLN 539
LQGFL+FH LL+ER + + N
Sbjct: 136 LQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSN 170
Score = 101 bits (242), Expect = 1e-22
Identities = 50/73 (68%), Positives = 55/73 (75%), Gaps = 5/73 (6%)
Frame = +1
Query: 43 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 207
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 208 TGAGKHVPRAVFV 246
TG GK VPR+++V
Sbjct: 60 TGQGKFVPRSIYV 72
Score = 100 bits (240), Expect = 2e-22
Identities = 42/56 (75%), Positives = 52/56 (92%)
Frame = +2
Query: 509 LSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDI 676
L+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDNEA YDI
Sbjct: 161 LNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDI 216
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 70.9 bits (166), Expect = 2e-13
Identities = 30/68 (44%), Positives = 44/68 (64%)
Frame = +3
Query: 255 PTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTG 434
P +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R+ A+ C
Sbjct: 70 PGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDA 129
Query: 435 LQGFLIFH 458
LQGF + H
Sbjct: 130 LQGFQLTH 137
Score = 53.2 bits (122), Expect = 4e-08
Identities = 23/54 (42%), Positives = 35/54 (64%)
Frame = +2
Query: 518 DYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDIW 679
+Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DNEA+ I+
Sbjct: 158 EYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIF 211
Score = 51.2 bits (117), Expect = 2e-07
Identities = 26/68 (38%), Positives = 34/68 (50%)
Frame = +1
Query: 43 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 222
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 223 HVPRAVFV 246
+VPRAV V
Sbjct: 59 YVPRAVLV 66
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 58.4 bits (135), Expect = 1e-09
Identities = 27/67 (40%), Positives = 41/67 (61%)
Frame = +1
Query: 46 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 225
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 226 VPRAVFV 246
+PRA+ +
Sbjct: 61 IPRAILI 67
Score = 48.0 bits (109), Expect = 2e-06
Identities = 27/87 (31%), Positives = 44/87 (50%), Gaps = 2/87 (2%)
Frame = +3
Query: 255 PTVVDEVRTGTYRQLFHPEQLITGKED--AANNYARGHYTIGKEIVDLVLDRIRKLADQC 428
P VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D I + AD
Sbjct: 71 PRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGS 129
Query: 429 TGLQGFLIFHXXXXXXXXXXXXLLMER 509
L+GF + H L+ER
Sbjct: 130 DSLEGFSLLHSIAGGTGSGLGSFLLER 156
Score = 37.1 bits (82), Expect = 0.003
Identities = 22/63 (34%), Positives = 36/63 (57%), Gaps = 1/63 (1%)
Frame = +2
Query: 491 FLIDGALSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAI 667
FL++ L+ Y KK ++++P Q VS VV+PYNS+L ++D ++DN A+
Sbjct: 152 FLLE-RLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLDNAAL 210
Query: 668 YDI 676
I
Sbjct: 211 AHI 213
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 30.3 bits (65), Expect = 0.32
Identities = 18/45 (40%), Positives = 26/45 (57%)
Frame = +2
Query: 122 STASSLMARCPQTRPSGVETILSTLSSARPELASTYPVLSSSILT 256
ST SSL + ++PS T ST SSA P S+ + SSS+++
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSSSYLSSSSVVS 217
Score = 25.4 bits (53), Expect = 9.2
Identities = 19/69 (27%), Positives = 34/69 (49%)
Frame = +2
Query: 50 SASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETILSTLSSARPELASTY 229
S+S + + S S P+ S ++ T+SSL + S + S+LSS+ ++
Sbjct: 206 SSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSSSSSSTASS 265
Query: 230 PVLSSSILT 256
SSSI++
Sbjct: 266 SSSSSSIIS 274
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 29.1 bits (62), Expect = 0.74
Identities = 24/72 (33%), Positives = 36/72 (50%)
Frame = +2
Query: 32 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCPQTRPSGVETILSTLSSARP 211
+K S + +++ P S SV P S T S++SS A PQ+ S ++S +SS
Sbjct: 456 VKQPTASVASSSVSVPSSSSVQPQSS-TPISSSSS--ASSPQSTLSTSSEVVSEVSSTLL 512
Query: 212 ELASTYPVLSSS 247
+S P SSS
Sbjct: 513 SGSSAIPSTSSS 524
>SPAC11E3.11c |||guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 942
Score = 27.1 bits (57), Expect = 3.0
Identities = 30/99 (30%), Positives = 50/99 (50%), Gaps = 6/99 (6%)
Frame = +2
Query: 29 KLKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARC----PQTRPSGVETILSTL 196
K KSK ++ + P S+SVM S T+ T +S +R R G+ T +S++
Sbjct: 85 KDKSKASMSTNDLPSHPRSQSVMGFSSSTSQLTGTSNSSRTRLNKDMRRDFGM-TSMSSI 143
Query: 197 SSARPELASTYPVLSSSILTYC--C**GPHWHIQTVVSS 307
+S+ P S PV S+ L++ GP + +T++SS
Sbjct: 144 TSSTP-TPSQLPVRPSTSLSFFDDIPLGPSFSAETILSS 181
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 141 WPDAHRQDHRGWRRFFQHFLQR 206
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 25.4 bits (53), Expect = 9.2
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = -1
Query: 294 VCMCQCGPHQQQ*VKIDEDSTGYV 223
VC + G Q +KID + TGYV
Sbjct: 1877 VCNDRSGHRQSSVIKIDPNETGYV 1900
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.2
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 32 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 154
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBC1683.13c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 618
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -3
Query: 253 QDRRRQHGVRACQLRSR*RKC*KNRLHPRWSCL 155
QD++ Q R R R RK +RLHP + C+
Sbjct: 7 QDKKNQEIFRISCQRCRQRKIKCDRLHPCFQCV 39
>SPCC1322.06 |kap113||karyopherin Kap113|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 983
Score = 25.4 bits (53), Expect = 9.2
Identities = 24/83 (28%), Positives = 36/83 (43%), Gaps = 6/83 (7%)
Frame = +2
Query: 14 FKHRKKLKSKCVSASLYTLAK----PESRS--VMPAGSFTAWSTASSLMARCPQTRPSGV 175
F + K KS S LYT+ + PES + A + WST + + P+T +
Sbjct: 648 FVNAIKAKSFEFSTFLYTVIEYCVNPESPEHVIFEADAMELWSTFLMYIQKLPETFTLLI 707
Query: 176 ETILSTLSSARPELASTYPVLSS 244
+L LS A L ++SS
Sbjct: 708 PHLLYHLSQATSTLPFVLMIVSS 730
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,275,665
Number of Sequences: 5004
Number of extensions: 68400
Number of successful extensions: 215
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 212
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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