BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30298 (668 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 174 8e-45 SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 170 2e-43 SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 80 2e-16 SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 60 4e-10 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.021 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 33 0.049 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 31 0.20 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 28 1.1 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 28 1.1 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 28 1.1 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 4.3 SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Sc... 26 4.3 SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharom... 26 4.3 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 26 5.6 SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 7.5 SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|c... 25 9.9 >SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 455 Score = 174 bits (424), Expect = 8e-45 Identities = 80/137 (58%), Positives = 96/137 (70%), Gaps = 3/137 (2%) Frame = +1 Query: 220 LPPYP---LPTGHVRPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCML 390 L PYP P P++SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+L Sbjct: 263 LVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRYMATCLL 322 Query: 391 YRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYRHPPWCPEATWPRFNVPSACC 570 YRGDV+P+DV AA+ +IK++RTIQFVDWCPTGFK+GI Y P P + + N Sbjct: 323 YRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSGIAKVNRAVCML 382 Query: 571 PTPPAIAEAWARLDHKF 621 +IAEAW+RLDHKF Sbjct: 383 SNTTSIAEAWSRLDHKF 399 Score = 158 bits (384), Expect = 6e-40 Identities = 72/83 (86%), Positives = 76/83 (91%) Frame = +2 Query: 5 TTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALN 184 TTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF G+LN Sbjct: 194 TTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFAGSLN 253 Query: 185 VDLTEFQTNLVPYPRIHFPLVTY 253 VDL EFQTNLVPYPRIHFPLVTY Sbjct: 254 VDLNEFQTNLVPYPRIHFPLVTY 276 Score = 33.5 bits (73), Expect = 0.028 Identities = 13/17 (76%), Positives = 14/17 (82%) Frame = +2 Query: 608 LTTSFDLMYAKRAFVHW 658 L FDLMY+KRAFVHW Sbjct: 395 LDHKFDLMYSKRAFVHW 411 >SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 449 Score = 170 bits (413), Expect = 2e-43 Identities = 80/137 (58%), Positives = 94/137 (68%), Gaps = 3/137 (2%) Frame = +1 Query: 220 LPPYP---LPTGHVRPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCML 390 L PYP P P++SA KA+HE SV EITN CFEP NQMVKCDPR G+YMA C+L Sbjct: 259 LVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRYMATCLL 318 Query: 391 YRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYRHPPWCPEATWPRFNVPSACC 570 YRGDV+P+DV AA+ TIK KRTIQFVDWCPTGFK+GI R P + + + Sbjct: 319 YRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSEIAKVDRAVCML 378 Query: 571 PTPPAIAEAWARLDHKF 621 +IAEAW+RLDHKF Sbjct: 379 SNTTSIAEAWSRLDHKF 395 Score = 154 bits (374), Expect = 1e-38 Identities = 69/83 (83%), Positives = 76/83 (91%) Frame = +2 Query: 5 TTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALN 184 TTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+G+LN Sbjct: 190 TTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFEGSLN 249 Query: 185 VDLTEFQTNLVPYPRIHFPLVTY 253 VDL EFQTNLVPYPRIHFPLVTY Sbjct: 250 VDLAEFQTNLVPYPRIHFPLVTY 272 Score = 33.5 bits (73), Expect = 0.028 Identities = 13/17 (76%), Positives = 14/17 (82%) Frame = +2 Query: 608 LTTSFDLMYAKRAFVHW 658 L FDLMY+KRAFVHW Sbjct: 391 LDHKFDLMYSKRAFVHW 407 >SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces pombe|chr 2|||Manual Length = 448 Score = 80.2 bits (189), Expect = 2e-16 Identities = 34/81 (41%), Positives = 52/81 (64%) Frame = +2 Query: 11 HTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVD 190 H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T S RF G LN D Sbjct: 190 HQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFPGELNSD 249 Query: 191 LTEFQTNLVPYPRIHFPLVTY 253 L + N+VP+PR+HF +V + Sbjct: 250 LRKLAVNMVPFPRLHFFMVGF 270 Score = 66.5 bits (155), Expect = 3e-12 Identities = 28/75 (37%), Positives = 45/75 (60%) Frame = +1 Query: 256 PVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIA 435 P+ + + + +SV E+T F+ N MV DPRHG+Y+ L+RG V K+V+ I Sbjct: 272 PLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRGKVSMKEVDEQIR 331 Query: 436 TIKTKRTIQFVDWCP 480 +++TK + FV+W P Sbjct: 332 SVQTKNSAYFVEWIP 346 >SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 59.7 bits (138), Expect = 4e-10 Identities = 26/76 (34%), Positives = 44/76 (57%) Frame = +2 Query: 26 HSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQ 205 ++D ++DN A+ I L + PT+ N+L+ ++S+ T +LR+ G +N DL Sbjct: 198 NADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYPGYMNNDLVSII 257 Query: 206 TNLVPYPRIHFPLVTY 253 +L+P PR HF L +Y Sbjct: 258 ASLIPSPRCHFLLTSY 273 Score = 41.5 bits (93), Expect = 1e-04 Identities = 18/75 (24%), Positives = 39/75 (52%), Gaps = 1/75 (1%) Frame = +1 Query: 295 SVAEITNACFEPANQMVKCDP-RHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVD 471 +V ++ P NQMV +P + +++ + +G+ P DV+ ++ I+ +R F+ Sbjct: 291 TVLDVMRRLLLPKNQMVSVNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIP 350 Query: 472 WCPTGFKVGINYRHP 516 W P +V ++ + P Sbjct: 351 WGPASIQVALSKKSP 365 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 33.9 bits (74), Expect = 0.021 Identities = 18/54 (33%), Positives = 30/54 (55%) Frame = -2 Query: 220 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 59 R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 32.7 bits (71), Expect = 0.049 Identities = 18/42 (42%), Positives = 24/42 (57%) Frame = +2 Query: 215 VPYPRIHFPLVTYGQSSLPRRPTMNSFPSPRSQTHASSPPTR 340 VP P P V+ +SS+P P N+ PSP S + AS+ P R Sbjct: 1239 VPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 30.7 bits (66), Expect = 0.20 Identities = 24/89 (26%), Positives = 39/89 (43%), Gaps = 2/89 (2%) Frame = +2 Query: 254 GQSSLPRRPTM--NSFPSPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TR 427 G S+ P PT+ S +P + T+ ++ + +TPV ++ CT TS P T Sbjct: 403 GNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTSVPY--TS 460 Query: 428 PSLPSKPSVLSNSSTGVQPVSRSVSTTAT 514 + S P +N +T S T+T Sbjct: 461 TPVTSTPLTTTNCTTSTSIPYTSTPVTST 489 Score = 27.5 bits (58), Expect = 1.8 Identities = 19/72 (26%), Positives = 35/72 (48%) Frame = +2 Query: 299 SPRSQTHASSPPTRW*NATPVMASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGV 478 +P + T+ ++ + +TPV ++ CT TS P T P S ++ S++ Sbjct: 525 TPVTTTNCTTSTSVLYTSTPVTSTPLATTNCTTSTSVPYTST-PVTSSNYTISSSTPVTS 583 Query: 479 QPVSRSVSTTAT 514 PV+ + TT+T Sbjct: 584 TPVTTTNCTTST 595 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/42 (33%), Positives = 18/42 (42%) Frame = +1 Query: 307 ITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAI 432 + ACFEP N C H K C L + KD N ++ Sbjct: 361 LCGACFEPINAKCYCG-LHSKTYPCSSLPSPSISKKDENGSV 401 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 28.3 bits (60), Expect = 1.1 Identities = 14/48 (29%), Positives = 23/48 (47%) Frame = -3 Query: 435 SDGRVHILGYDVTTVQHTASHVLAMTGVAFHHLVGGLEACVCDLGDGK 292 S G +LGY ++ A++V+A + V HL+ G D + K Sbjct: 407 SAGLTSLLGYHLSVKTPQATYVVARSIVMLDHLIDGYSMAFPDFSESK 454 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 28.3 bits (60), Expect = 1.1 Identities = 26/68 (38%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Frame = +2 Query: 314 THASSPPTRW*NATPVM-ASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVS 490 T ASS P N+T AS+ TS T PS + SVL SS P+S Sbjct: 368 TSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSSTAPSYNTS-SVLPTSSVSSTPLS 426 Query: 491 RSVSTTAT 514 + STTAT Sbjct: 427 SANSTTAT 434 Score = 28.3 bits (60), Expect = 1.1 Identities = 26/68 (38%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Frame = +2 Query: 314 THASSPPTRW*NATPVM-ASTWLAVCCTVVTSYPRM*TRPSLPSKPSVLSNSSTGVQPVS 490 T ASS P N+T AS+ TS T PS + SVL SS P+S Sbjct: 482 TSASSTPLTSVNSTSATSASSTPLTSANSTTSTSVSSTAPSYNTS-SVLPTSSVSSTPLS 540 Query: 491 RSVSTTAT 514 + STTAT Sbjct: 541 SANSTTAT 548 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 4.3 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +2 Query: 257 QSSLPRRPTMNSFPSPRSQTHASSPPTR 340 + SLPRRP+ +P S T ++ PP + Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765 >SPBC428.07 |meu6||meiotic chromosome segregation protein Meu6|Schizosaccharomyces pombe|chr 2|||Manual Length = 651 Score = 26.2 bits (55), Expect = 4.3 Identities = 16/63 (25%), Positives = 27/63 (42%), Gaps = 1/63 (1%) Frame = +2 Query: 176 ALNVDLTEFQTNLVPYPRIHFPLVTYGQS-SLPRRPTMNSFPSPRSQTHASSPPTRW*NA 352 A +V++ E +TN +P HFP T + + +P S + T+ N Sbjct: 368 AQSVEVPENETNQIPTTEEHFPATTEEVAPASEEKPATGPAEESTSTQNVEQASTQNDNG 427 Query: 353 TPV 361 TP+ Sbjct: 428 TPI 430 >SPAC3C7.09 |set8||lysine methyltransferase Set8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 429 Score = 26.2 bits (55), Expect = 4.3 Identities = 7/17 (41%), Positives = 13/17 (76%) Frame = -3 Query: 561 RRHVEPWPSRLRAPRWV 511 +R+ + WPS++ P+WV Sbjct: 153 QRYSDSWPSKITLPKWV 169 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 25.8 bits (54), Expect = 5.6 Identities = 10/20 (50%), Positives = 11/20 (55%) Frame = -2 Query: 412 WVRRHHGTAYSKPCTCHDGG 353 W R H Y+KPC DGG Sbjct: 16 WRRDHPFGFYAKPCKSSDGG 35 >SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.4 bits (53), Expect = 7.5 Identities = 15/58 (25%), Positives = 27/58 (46%) Frame = -2 Query: 214 QVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDGFIVDHE 41 Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658 >SPAP8A3.13c |||Vid 24 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 25.0 bits (52), Expect = 9.9 Identities = 14/49 (28%), Positives = 27/49 (55%) Frame = +2 Query: 431 SLPSKPSVLSNSSTGVQPVSRSVSTTATHRGARRRLGQGSTCRLHVVQH 577 SL + P+VL + P ++ S++A+ G+R + +C L++V H Sbjct: 280 SLDNNPAVLDLNG----PPNQESSSSASSYGSRTQTPNARSCSLNIVFH 324 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,138,582 Number of Sequences: 5004 Number of extensions: 69766 Number of successful extensions: 289 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 254 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 285 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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