Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30294
(753 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC6G10.08 |idp1||isocitrate dehydrogenase Idp1|Schizosaccharom... 128 6e-31
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 31 0.23
SPAC1783.03 |fta2|sma2|Sim4 and Mal2 associated |Schizosaccharom... 31 0.23
SPAC23D3.13c |||guanyl-nucleotide exchange factor|Schizosaccharo... 27 2.9
SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 27 3.8
SPBC336.12c |cdc10||MBF transcription factor complex subunit Cdc... 27 3.8
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 5.0
SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|c... 26 6.6
SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|ch... 26 6.6
SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 26 6.6
SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr 2|||... 25 8.8
>SPAC6G10.08 |idp1||isocitrate dehydrogenase
Idp1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 418
Score = 128 bits (310), Expect = 6e-31
Identities = 54/78 (69%), Positives = 69/78 (88%)
Frame = +1
Query: 508 GAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDRE 687
G+GVA++M+NTD SI FAHSSF+ AL +K PLYLSTKNTILKKYDGRFKD FQ++Y+ +
Sbjct: 185 GSGVAMSMYNTDDSIRGFAHSSFQMALQKKMPLYLSTKNTILKKYDGRFKDTFQEVYESD 244
Query: 688 YKKQFEDAGIWYEHRLID 741
YK++FE+ G+WY+HRLID
Sbjct: 245 YKQKFEELGLWYQHRLID 262
Score = 124 bits (298), Expect = 2e-29
Identities = 63/120 (52%), Positives = 86/120 (71%), Gaps = 1/120 (0%)
Frame = +3
Query: 18 PVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 197
PVV++ GDEMTR+IW +I+EKL+LP++DI+L YDLG+E RDKT+DQ+T+D A+AI K +
Sbjct: 19 PVVEMDGDEMTRVIWKIIREKLVLPYMDIKLDYYDLGIEARDKTNDQITVDAAKAILKND 78
Query: 198 VGIKCATITPDEKRVEEFK*RKCGKALMGP-SVIFLVVLFSGRLLFARIYRDL*QGGTNP 374
VGIKCATITPDE RV+E+ +K K+ G I +F +L I + + G TNP
Sbjct: 79 VGIKCATITPDEARVKEYNLKKMWKSPNGTIRNILNGTVFREPILIKNIPKYI-PGWTNP 137
Score = 115 bits (276), Expect = 8e-27
Identities = 50/85 (58%), Positives = 60/85 (70%)
Frame = +2
Query: 254 MKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPIIIGRHAHADQYKATDFVVP 433
+KKMWKSPNGTIRNIL GTVFRE I+ KNIP+ + GW PI IGRHA DQYK+TD V
Sbjct: 98 LKKMWKSPNGTIRNILNGTVFREPILIKNIPKYIPGWTNPICIGRHAFGDQYKSTDLVAS 157
Query: 434 GAGTLEIIFKPESGEAIKHVVHEYK 508
G G LE+ F P+ + K + Y+
Sbjct: 158 GPGKLELSFTPKGNPSAKETYNVYE 182
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 30.7 bits (66), Expect = 0.23
Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 1/70 (1%)
Frame = +3
Query: 30 ILGDEMTRIIWDLIKEKLILPFLDIELHVYDL-GMENRDKTDDQVTIDCAEAIKKYNVGI 206
I GD + R + + E + IE D+ GME +K+ + +++K+ VG+
Sbjct: 26 IPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSLKRNKVGL 85
Query: 207 KCATITPDEK 236
K TP EK
Sbjct: 86 KGILFTPFEK 95
>SPAC1783.03 |fta2|sma2|Sim4 and Mal2 associated
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 351
Score = 30.7 bits (66), Expect = 0.23
Identities = 23/90 (25%), Positives = 37/90 (41%), Gaps = 8/90 (8%)
Frame = -3
Query: 496 NYMFYCLTRFRFEDYFKSTSTWDNKVSCF--------VLISMSMTSNDDGFVPPCYKSRY 341
+Y + C T F + K ++ + CF + I + +D GFV P Y Y
Sbjct: 242 HYSYRCNTWFYLSKHMKKVASANMDAQCFYVQNSFYKITILYEIKFDDLGFVQPNYCISY 301
Query: 340 ILANNSLPENSTTKNITDGPIRAFPHFLHL 251
IL N + S T + + + F F+ L
Sbjct: 302 ILKNQNEKIVSITSKLLNQLDKRFSEFVSL 331
>SPAC23D3.13c |||guanyl-nucleotide exchange
factor|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1616
Score = 27.1 bits (57), Expect = 2.9
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = -3
Query: 337 LANNSLPENSTTKNITDGPIRAFPHFLHLNSSTLFS 230
L+N ++P N + N+T + H L++ST+FS
Sbjct: 547 LSNKAVPSNLLSGNMTHSASSSISHNGRLSTSTMFS 582
>SPCC550.03c |||RNA helicase involved in mRNA
catabolism|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1213
Score = 26.6 bits (56), Expect = 3.8
Identities = 16/57 (28%), Positives = 28/57 (49%)
Frame = +1
Query: 547 SIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDREYKKQFEDAGI 717
S+ AH+S + +Y + L+ K+ Y K + + R++K +FED GI
Sbjct: 297 SVFVAAHTSAGKTVVAEYAIALAQKHMTKAIYTSPIKALSNQKF-RDFKHKFEDVGI 352
>SPBC336.12c |cdc10||MBF transcription factor complex subunit
Cdc10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 767
Score = 26.6 bits (56), Expect = 3.8
Identities = 16/48 (33%), Positives = 28/48 (58%)
Frame = -3
Query: 430 DNKVSCFVLISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNITD 287
D+K + L+S +++S + VPP KSR I+A+ + +S K+ D
Sbjct: 536 DSKQTKVSLMSENLSSKEKTAVPPRQKSRDIIASVTDVISSLDKDFQD 583
>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
Length = 596
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +1
Query: 616 TKNTILKKYDGRFKDIFQDIYDREYKKQFEDAG 714
T+ T+L DG+FKDIF + + ++E G
Sbjct: 414 TQETLLGLLDGKFKDIFDKCIIIDCRFEYEYLG 446
>SPAC15E1.04 |||thymidylate synthase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 625
Score = 25.8 bits (54), Expect = 6.6
Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 2/54 (3%)
Frame = -3
Query: 370 FVPPCYKSRYILANNSLPENSTTKNITDGPIRAFPHFLH--LNSSTLFSSGVMV 215
F PP + R+ L NN+LP +T + G + F+H N++ L G+ +
Sbjct: 359 FAPP--QLRFSLRNNTLPLLTTKRVFLRGVLEELLWFIHGDTNANHLSEKGIHI 410
>SPAC869.05c |||sulfate transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 840
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -1
Query: 384 PMMMGLSHPVTSLGIFLQIIASLKTVP 304
P +MG + VT+ ++ II SLK +P
Sbjct: 253 PALMGYKNKVTAKATYMVIIQSLKHLP 279
>SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1687
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +3
Query: 78 KLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIK 188
+LI+ F D ELH + N+ + D AEA+K
Sbjct: 400 RLIMKFSDSELHTFLFHCVNKIVANTTENSDSAEAVK 436
>SPBPJ4664.02 |||glycoprotein |Schizosaccharomyces pombe|chr
2|||Manual
Length = 3971
Score = 25.4 bits (53), Expect = 8.8
Identities = 28/106 (26%), Positives = 52/106 (49%)
Frame = -3
Query: 331 NNSLPENSTTKNITDGPIRAFPHFLHLNSSTLFSSGVMVAHLMPTLYFFMASAQSIVT*S 152
N+S P S+T + PI + LNSST +S +V P + ++ + +T S
Sbjct: 799 NSSTPITSSTVLNSSTPITSSSV---LNSSTPITSSTVVNTSTPITSSTVVNSSTPITSS 855
Query: 151 SVLSRFSIPKS*TCNSMSKKGRISFSLIRSQIILVISSPRMSTTGP 14
SVL+ S P + + +++ I+ S + + + SS ++T+ P
Sbjct: 856 SVLNS-STPIT-SSTALNTSTPITSSSVLNSSTPITSSTVVNTSTP 899
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,103,122
Number of Sequences: 5004
Number of extensions: 64174
Number of successful extensions: 201
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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