BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30294 (753 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 122 2e-28 At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA... 119 2e-27 At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 118 6e-27 At3g21890.1 68416.m02760 zinc finger (B-box type) family protein... 30 1.9 At2g46980.2 68415.m05869 expressed protein 29 2.5 At2g46980.1 68415.m05868 expressed protein 29 2.5 At2g16750.1 68415.m01921 protein kinase family protein contains ... 29 3.3 At4g40050.1 68417.m05671 expressed protein 28 5.8 At5g63070.1 68418.m07914 40S ribosomal protein S15, putative 28 7.7 At5g08390.1 68418.m00988 transducin family protein / WD-40 repea... 28 7.7 >At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to isocitrate dehydrogenase (NADP+) [Nicotiana tabacum] GI:3021512; contains Pfam domain PF00180: dehydrogenase, isocitrate/isopropylmalate family Length = 485 Score = 122 bits (295), Expect = 2e-28 Identities = 54/86 (62%), Positives = 68/86 (79%), Gaps = 1/86 (1%) Frame = +2 Query: 254 MKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPIIIGRHAHADQYKATDFVVP 433 +K MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+KPI IGRHA DQY+ATD V+ Sbjct: 160 LKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPICIGRHAFGDQYRATDTVIK 219 Query: 434 GAGTLEIIFKPESGEA-IKHVVHEYK 508 G G L+++F PE G A ++ V+++K Sbjct: 220 GPGKLKMVFVPEDGNAPVELDVYDFK 245 Score = 121 bits (291), Expect = 6e-28 Identities = 54/79 (68%), Positives = 65/79 (82%) Frame = +1 Query: 505 QGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDR 684 +G GVALAM+N D SI FA SS AL +K+PLYLSTKNTILKKYDGRFKDIFQ++Y+ Sbjct: 245 KGPGVALAMYNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQEVYEA 304 Query: 685 EYKKQFEDAGIWYEHRLID 741 +K++FE+ IWYEHRLID Sbjct: 305 NWKQKFEEHSIWYEHRLID 323 Score = 120 bits (289), Expect = 1e-27 Identities = 52/78 (66%), Positives = 67/78 (85%) Frame = +3 Query: 18 PVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 197 P+V++ GDEMTR+IW +IKEKLILP+LD+++ +DLG+ NRD TDD+VT++ AEA KYN Sbjct: 81 PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYN 140 Query: 198 VGIKCATITPDEKRVEEF 251 V IKCATITPDE RV+EF Sbjct: 141 VAIKCATITPDEGRVKEF 158 >At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to NADP-isocitrate dehydrogenase GI:5764653 from [Citrus limon]; Nicotiana tabacum SP|P50218 Length = 416 Score = 119 bits (286), Expect = 2e-27 Identities = 57/95 (60%), Positives = 72/95 (75%), Gaps = 1/95 (1%) Frame = +3 Query: 3 KIKA-GPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 179 KIK PVV++ GDEMTR+IW IK+KLI PFL++++ +DLG+ NRD TDD+VTI+ AE Sbjct: 5 KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64 Query: 180 AIKKYNVGIKCATITPDEKRVEEFK*RKCGKALMG 284 A KYNV IKCATITPDE RV EF +K ++ G Sbjct: 65 ATLKYNVAIKCATITPDEARVREFGLKKMWRSPNG 99 Score = 118 bits (284), Expect = 4e-27 Identities = 53/78 (67%), Positives = 63/78 (80%) Frame = +2 Query: 254 MKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPIIIGRHAHADQYKATDFVVP 433 +KKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV GW KPI IGRHA DQY+ATD +V Sbjct: 90 LKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVPGWTKPICIGRHAFGDQYRATDLIVN 149 Query: 434 GAGTLEIIFKPESGEAIK 487 G L+++F+P SG + K Sbjct: 150 EPGKLKLVFEP-SGSSQK 166 Score = 117 bits (282), Expect = 7e-27 Identities = 53/78 (67%), Positives = 63/78 (80%) Frame = +1 Query: 508 GAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDRE 687 G GVALAM+NTD SI FA SS A +K+PLYLSTKNTILK YDGRFKDIFQ++Y+ Sbjct: 176 GGGVALAMYNTDESIRAFAESSMYTAYQKKWPLYLSTKNTILKIYDGRFKDIFQEVYEAN 235 Query: 688 YKKQFEDAGIWYEHRLID 741 ++ ++E AGIWYEHRLID Sbjct: 236 WRSKYEAAGIWYEHRLID 253 >At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+ isocitrate dehydrogenase, putative strong similarity to isocitrate dehydrogenase SP|Q40345 from [Medicago sativa] Length = 410 Score = 118 bits (283), Expect = 6e-27 Identities = 54/84 (64%), Positives = 69/84 (82%), Gaps = 1/84 (1%) Frame = +3 Query: 3 KIK-AGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 179 KIK A P+V++ GDEMTR+IW IK+KLI PF+++++ +DLG+ +RD TDD+VTI+ AE Sbjct: 5 KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64 Query: 180 AIKKYNVGIKCATITPDEKRVEEF 251 A KKYNV IKCATITPDE RV EF Sbjct: 65 ATKKYNVAIKCATITPDEGRVTEF 88 Score = 117 bits (282), Expect = 7e-27 Identities = 51/76 (67%), Positives = 64/76 (84%) Frame = +1 Query: 514 GVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDREYK 693 GVA+AM+NTD SI FA +S A ++K+PLYLSTKNTILKKYDGRFKDIFQ++Y+ +K Sbjct: 177 GVAMAMYNTDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWK 236 Query: 694 KQFEDAGIWYEHRLID 741 +++ AGIWYEHRLID Sbjct: 237 SKYDAAGIWYEHRLID 252 Score = 115 bits (276), Expect = 4e-26 Identities = 49/75 (65%), Positives = 60/75 (80%) Frame = +2 Query: 254 MKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPIIIGRHAHADQYKATDFVVP 433 +K+MW+SPNGTIRNIL GTVFRE IICKN+P+LV GW KPI IGRHA DQY+ATD V+ Sbjct: 90 LKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIK 149 Query: 434 GAGTLEIIFKPESGE 478 G G L + F+ + G+ Sbjct: 150 GPGKLTMTFEGKDGK 164 >At3g21890.1 68416.m02760 zinc finger (B-box type) family protein contains Pfam profile: PF01760 CONSTANS family zinc finger Length = 121 Score = 29.9 bits (64), Expect = 1.9 Identities = 11/24 (45%), Positives = 14/24 (58%) Frame = -3 Query: 715 YQHPQTVSCTPCHKYLERCL*NDH 644 ++H + V CT C K RCL DH Sbjct: 65 WRHVRRVLCTSCQKLTRRCLVGDH 88 >At2g46980.2 68415.m05869 expressed protein Length = 516 Score = 29.5 bits (63), Expect = 2.5 Identities = 13/38 (34%), Positives = 21/38 (55%) Frame = -3 Query: 403 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 290 +S SNDD + P + S I ++ PEN+T + +T Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266 >At2g46980.1 68415.m05868 expressed protein Length = 516 Score = 29.5 bits (63), Expect = 2.5 Identities = 13/38 (34%), Positives = 21/38 (55%) Frame = -3 Query: 403 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 290 +S SNDD + P + S I ++ PEN+T + +T Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266 >At2g16750.1 68415.m01921 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 617 Score = 29.1 bits (62), Expect = 3.3 Identities = 16/45 (35%), Positives = 22/45 (48%) Frame = +2 Query: 377 IIGRHAHADQYKATDFVVPGAGTLEIIFKPESGEAIKHVVHEYKV 511 +IG+ + YK F+ G G I KP EA+K VHE + Sbjct: 282 LIGKGGCNEVYKG--FLEDGKGVAVKILKPSVKEAVKEFVHEVSI 324 >At4g40050.1 68417.m05671 expressed protein Length = 599 Score = 28.3 bits (60), Expect = 5.8 Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 3/53 (5%) Frame = -3 Query: 409 VLISMSMTSNDDGFVPPCYKS-RYILANNSLPENSTTKNITD--GPIRAFPHF 260 VL S S T +DD ++P C+ + +L++ S P S+ + + + G ++F HF Sbjct: 545 VLTSFSRTEDDDPYLPQCHPNLPELLSSVSKPVQSSVQRLAEHLGVAKSF-HF 596 >At5g63070.1 68418.m07914 40S ribosomal protein S15, putative Length = 160 Score = 27.9 bits (59), Expect = 7.7 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = +1 Query: 322 GYYLQEYTETCNRVGQTHHHWTSCSC 399 G+YL E++ TC +V HH C C Sbjct: 124 GHYLAEFSMTCKKV--NHHRPRICGC 147 >At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat family protein similar to katanin p80 subunit [Strongylocentrotus purpuratus] GI:3005601; contains Pfam profile PF00400: WD domain, G-beta repeat Length = 871 Score = 27.9 bits (59), Expect = 7.7 Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%) Frame = +3 Query: 36 GDEMTRIIWDLIKEKLILPF---------LDIELHVYDLGMENRDKTDDQVTIDCAEAIK 188 G++ +WDL KL+ F LD H + L + DKT ++ E I Sbjct: 255 GEDNVVKVWDLTAGKLLHEFKSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 314 Query: 189 K---YNVGIKCATITPDEKRV 242 G++C T PD K V Sbjct: 315 SGGTETTGVRCLTFNPDGKSV 335 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,084,157 Number of Sequences: 28952 Number of extensions: 331741 Number of successful extensions: 873 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 852 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 873 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1672953192 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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