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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30294
         (753 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA...   122   2e-28
At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NA...   119   2e-27
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA...   118   6e-27
At3g21890.1 68416.m02760 zinc finger (B-box type) family protein...    30   1.9  
At2g46980.2 68415.m05869 expressed protein                             29   2.5  
At2g46980.1 68415.m05868 expressed protein                             29   2.5  
At2g16750.1 68415.m01921 protein kinase family protein contains ...    29   3.3  
At4g40050.1 68417.m05671 expressed protein                             28   5.8  
At5g63070.1 68418.m07914 40S ribosomal protein S15, putative           28   7.7  
At5g08390.1 68418.m00988 transducin family protein / WD-40 repea...    28   7.7  

>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
           GI:3021512; contains Pfam domain PF00180: dehydrogenase,
           isocitrate/isopropylmalate family
          Length = 485

 Score =  122 bits (295), Expect = 2e-28
 Identities = 54/86 (62%), Positives = 68/86 (79%), Gaps = 1/86 (1%)
 Frame = +2

Query: 254 MKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPIIIGRHAHADQYKATDFVVP 433
           +K MW+SPNGTIRNIL GTVFRE I+C NIPRLV GW+KPI IGRHA  DQY+ATD V+ 
Sbjct: 160 LKSMWRSPNGTIRNILDGTVFREPIMCSNIPRLVPGWEKPICIGRHAFGDQYRATDTVIK 219

Query: 434 GAGTLEIIFKPESGEA-IKHVVHEYK 508
           G G L+++F PE G A ++  V+++K
Sbjct: 220 GPGKLKMVFVPEDGNAPVELDVYDFK 245



 Score =  121 bits (291), Expect = 6e-28
 Identities = 54/79 (68%), Positives = 65/79 (82%)
 Frame = +1

Query: 505 QGAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDR 684
           +G GVALAM+N D SI  FA SS   AL +K+PLYLSTKNTILKKYDGRFKDIFQ++Y+ 
Sbjct: 245 KGPGVALAMYNVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQEVYEA 304

Query: 685 EYKKQFEDAGIWYEHRLID 741
            +K++FE+  IWYEHRLID
Sbjct: 305 NWKQKFEEHSIWYEHRLID 323



 Score =  120 bits (289), Expect = 1e-27
 Identities = 52/78 (66%), Positives = 67/78 (85%)
 Frame = +3

Query: 18  PVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAEAIKKYN 197
           P+V++ GDEMTR+IW +IKEKLILP+LD+++  +DLG+ NRD TDD+VT++ AEA  KYN
Sbjct: 81  PIVEMDGDEMTRVIWSMIKEKLILPYLDLDIKYFDLGILNRDATDDKVTVESAEAALKYN 140

Query: 198 VGIKCATITPDEKRVEEF 251
           V IKCATITPDE RV+EF
Sbjct: 141 VAIKCATITPDEGRVKEF 158


>At1g54340.1 68414.m06195 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           NADP-isocitrate dehydrogenase GI:5764653 from [Citrus
           limon]; Nicotiana tabacum SP|P50218
          Length = 416

 Score =  119 bits (286), Expect = 2e-27
 Identities = 57/95 (60%), Positives = 72/95 (75%), Gaps = 1/95 (1%)
 Frame = +3

Query: 3   KIKA-GPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 179
           KIK   PVV++ GDEMTR+IW  IK+KLI PFL++++  +DLG+ NRD TDD+VTI+ AE
Sbjct: 5   KIKVINPVVEMDGDEMTRVIWKFIKDKLIFPFLELDIKYFDLGLPNRDFTDDKVTIETAE 64

Query: 180 AIKKYNVGIKCATITPDEKRVEEFK*RKCGKALMG 284
           A  KYNV IKCATITPDE RV EF  +K  ++  G
Sbjct: 65  ATLKYNVAIKCATITPDEARVREFGLKKMWRSPNG 99



 Score =  118 bits (284), Expect = 4e-27
 Identities = 53/78 (67%), Positives = 63/78 (80%)
 Frame = +2

Query: 254 MKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPIIIGRHAHADQYKATDFVVP 433
           +KKMW+SPNGTIRNIL GTVFRE IIC+NIPRLV GW KPI IGRHA  DQY+ATD +V 
Sbjct: 90  LKKMWRSPNGTIRNILNGTVFREPIICRNIPRLVPGWTKPICIGRHAFGDQYRATDLIVN 149

Query: 434 GAGTLEIIFKPESGEAIK 487
             G L+++F+P SG + K
Sbjct: 150 EPGKLKLVFEP-SGSSQK 166



 Score =  117 bits (282), Expect = 7e-27
 Identities = 53/78 (67%), Positives = 63/78 (80%)
 Frame = +1

Query: 508 GAGVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDRE 687
           G GVALAM+NTD SI  FA SS   A  +K+PLYLSTKNTILK YDGRFKDIFQ++Y+  
Sbjct: 176 GGGVALAMYNTDESIRAFAESSMYTAYQKKWPLYLSTKNTILKIYDGRFKDIFQEVYEAN 235

Query: 688 YKKQFEDAGIWYEHRLID 741
           ++ ++E AGIWYEHRLID
Sbjct: 236 WRSKYEAAGIWYEHRLID 253


>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
           isocitrate dehydrogenase, putative strong similarity to
           isocitrate dehydrogenase SP|Q40345 from [Medicago
           sativa]
          Length = 410

 Score =  118 bits (283), Expect = 6e-27
 Identities = 54/84 (64%), Positives = 69/84 (82%), Gaps = 1/84 (1%)
 Frame = +3

Query: 3   KIK-AGPVVDILGDEMTRIIWDLIKEKLILPFLDIELHVYDLGMENRDKTDDQVTIDCAE 179
           KIK A P+V++ GDEMTR+IW  IK+KLI PF+++++  +DLG+ +RD TDD+VTI+ AE
Sbjct: 5   KIKVANPIVEMDGDEMTRVIWKSIKDKLITPFVELDIKYFDLGLPHRDATDDKVTIESAE 64

Query: 180 AIKKYNVGIKCATITPDEKRVEEF 251
           A KKYNV IKCATITPDE RV EF
Sbjct: 65  ATKKYNVAIKCATITPDEGRVTEF 88



 Score =  117 bits (282), Expect = 7e-27
 Identities = 51/76 (67%), Positives = 64/76 (84%)
 Frame = +1

Query: 514 GVALAMFNTDASIIDFAHSSFKFALDRKYPLYLSTKNTILKKYDGRFKDIFQDIYDREYK 693
           GVA+AM+NTD SI  FA +S   A ++K+PLYLSTKNTILKKYDGRFKDIFQ++Y+  +K
Sbjct: 177 GVAMAMYNTDESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWK 236

Query: 694 KQFEDAGIWYEHRLID 741
            +++ AGIWYEHRLID
Sbjct: 237 SKYDAAGIWYEHRLID 252



 Score =  115 bits (276), Expect = 4e-26
 Identities = 49/75 (65%), Positives = 60/75 (80%)
 Frame = +2

Query: 254 MKKMWKSPNGTIRNILGGTVFREAIICKNIPRLVTGWDKPIIIGRHAHADQYKATDFVVP 433
           +K+MW+SPNGTIRNIL GTVFRE IICKN+P+LV GW KPI IGRHA  DQY+ATD V+ 
Sbjct: 90  LKQMWRSPNGTIRNILNGTVFREPIICKNVPKLVPGWTKPICIGRHAFGDQYRATDAVIK 149

Query: 434 GAGTLEIIFKPESGE 478
           G G L + F+ + G+
Sbjct: 150 GPGKLTMTFEGKDGK 164


>At3g21890.1 68416.m02760 zinc finger (B-box type) family protein
           contains Pfam profile: PF01760 CONSTANS family zinc
           finger
          Length = 121

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 11/24 (45%), Positives = 14/24 (58%)
 Frame = -3

Query: 715 YQHPQTVSCTPCHKYLERCL*NDH 644
           ++H + V CT C K   RCL  DH
Sbjct: 65  WRHVRRVLCTSCQKLTRRCLVGDH 88


>At2g46980.2 68415.m05869 expressed protein
          Length = 516

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = -3

Query: 403 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 290
           +S    SNDD  + P + S  I  ++  PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266


>At2g46980.1 68415.m05868 expressed protein
          Length = 516

 Score = 29.5 bits (63), Expect = 2.5
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = -3

Query: 403 ISMSMTSNDDGFVPPCYKSRYILANNSLPENSTTKNIT 290
           +S    SNDD  + P + S  I  ++  PEN+T + +T
Sbjct: 229 LSQDKGSNDDPLIKPRHNSDSIETDSESPENATRRPVT 266


>At2g16750.1 68415.m01921 protein kinase family protein contains
           Pfam domain, PF00069: Protein kinase domain
          Length = 617

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 16/45 (35%), Positives = 22/45 (48%)
 Frame = +2

Query: 377 IIGRHAHADQYKATDFVVPGAGTLEIIFKPESGEAIKHVVHEYKV 511
           +IG+    + YK   F+  G G    I KP   EA+K  VHE  +
Sbjct: 282 LIGKGGCNEVYKG--FLEDGKGVAVKILKPSVKEAVKEFVHEVSI 324


>At4g40050.1 68417.m05671 expressed protein
          Length = 599

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 17/53 (32%), Positives = 31/53 (58%), Gaps = 3/53 (5%)
 Frame = -3

Query: 409 VLISMSMTSNDDGFVPPCYKS-RYILANNSLPENSTTKNITD--GPIRAFPHF 260
           VL S S T +DD ++P C+ +   +L++ S P  S+ + + +  G  ++F HF
Sbjct: 545 VLTSFSRTEDDDPYLPQCHPNLPELLSSVSKPVQSSVQRLAEHLGVAKSF-HF 596


>At5g63070.1 68418.m07914 40S ribosomal protein S15, putative
          Length = 160

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 11/26 (42%), Positives = 15/26 (57%)
 Frame = +1

Query: 322 GYYLQEYTETCNRVGQTHHHWTSCSC 399
           G+YL E++ TC +V   HH    C C
Sbjct: 124 GHYLAEFSMTCKKV--NHHRPRICGC 147


>At5g08390.1 68418.m00988 transducin family protein / WD-40 repeat
           family protein similar to katanin p80 subunit
           [Strongylocentrotus purpuratus] GI:3005601; contains
           Pfam profile PF00400: WD domain, G-beta repeat
          Length = 871

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 23/81 (28%), Positives = 33/81 (40%), Gaps = 12/81 (14%)
 Frame = +3

Query: 36  GDEMTRIIWDLIKEKLILPF---------LDIELHVYDLGMENRDKTDDQVTIDCAEAIK 188
           G++    +WDL   KL+  F         LD   H + L   + DKT     ++  E I 
Sbjct: 255 GEDNVVKVWDLTAGKLLHEFKSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 314

Query: 189 K---YNVGIKCATITPDEKRV 242
                  G++C T  PD K V
Sbjct: 315 SGGTETTGVRCLTFNPDGKSV 335


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,084,157
Number of Sequences: 28952
Number of extensions: 331741
Number of successful extensions: 873
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 852
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 873
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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