BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30293 (726 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g26125.1 68416.m03258 cytochrome P450, putative 35 0.048 At1g62350.1 68414.m07035 pentatricopeptide (PPR) repeat-containi... 32 0.45 At1g12200.1 68414.m01412 flavin-containing monooxygenase family ... 29 2.4 At4g20240.1 68417.m02957 cytochrome P450, putative similar to |C... 29 4.1 At3g11110.1 68416.m01345 zinc finger (C3HC4-type RING finger) fa... 29 4.1 At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 29 4.1 At1g16460.2 68414.m01969 mercaptopyruvate sulfurtransferase (MST... 29 4.1 At1g16460.1 68414.m01968 mercaptopyruvate sulfurtransferase (MST... 29 4.1 At3g58890.1 68416.m06563 syntaxin-related family protein contain... 28 5.5 At2g14140.1 68415.m01575 hypothetical protein similar to At2g049... 28 5.5 At1g15420.1 68414.m01850 expressed protein 28 5.5 At5g54880.1 68418.m06836 DTW domain-containing protein contains ... 28 7.2 At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF051... 28 7.2 At2g18890.1 68415.m02204 protein kinase family protein contains ... 28 7.2 At1g58170.1 68414.m06599 disease resistance-responsive protein-r... 27 9.6 >At3g26125.1 68416.m03258 cytochrome P450, putative Length = 541 Score = 35.1 bits (77), Expect = 0.048 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = -3 Query: 328 ALLGLFDYQCFEAGVINQGTTARWVPFSIL*DFLMANISRFFW 200 ALLGLF + C +A + N+G +W F I +F + F W Sbjct: 26 ALLGLFLFGCLQAKLTNKGEPVQWPVFGITPEFFLHVHDMFGW 68 >At1g62350.1 68414.m07035 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 225 Score = 31.9 bits (69), Expect = 0.45 Identities = 22/74 (29%), Positives = 35/74 (47%), Gaps = 3/74 (4%) Frame = +2 Query: 161 LEEDKEYNGSRSSPEEPRNVRHQEILQYRERYPSRRRSLVDD--ARFETLVVKQTKQSVL 334 LE + Y R SP+ P ++ + IL+ YP R + DD F ++V + + Sbjct: 152 LEAMRLYGEMRESPDRPLSLPFRVILKGLVPYPELREKVKDDFLELFPGMIVYDPPEDIC 211 Query: 335 EEARARA-NDSGLE 373 E++ A DS LE Sbjct: 212 EDSDEEARTDSDLE 225 >At1g12200.1 68414.m01412 flavin-containing monooxygenase family protein / FMO family protein low similarity to FMO2 from Homo sapiens [SP|Q99518]; contains Pfam profile: PF00743 Flavin-binding monooxygenase-like Length = 465 Score = 29.5 bits (63), Expect = 2.4 Identities = 20/76 (26%), Positives = 35/76 (46%) Frame = +2 Query: 173 KEYNGSRSSPEEPRNVRHQEILQYRERYPSRRRSLVDDARFETLVVKQTKQSVLEEARAR 352 + ++GSR P H E+L Y + ++ + + RFET VVK + + E R + Sbjct: 97 RPHDGSRDPRRHPA---HTEVLAYLRDF-AKEFDIEEMVRFETEVVKAEQVAAEGEERGK 152 Query: 353 ANDSGLESDFIQDGVH 400 SD + D ++ Sbjct: 153 WRVESRSSDGVVDEIY 168 >At4g20240.1 68417.m02957 cytochrome P450, putative similar to |C71R_ARATH Cytochrome P450 71A27 (SP:O65438) [Arabidopsis thaliana] Length = 865 Score = 28.7 bits (61), Expect = 4.1 Identities = 20/75 (26%), Positives = 34/75 (45%), Gaps = 4/75 (5%) Frame = +3 Query: 231 KSYSIENGTHLAVVPWLMTPASKHW*SN----KPSRAYLKRLAPEPMTLAWNLISSKMEC 398 K Y I GTH+ + W + W + +P R + +RL P + ++L+ SK+ Sbjct: 378 KGYDISAGTHVIINAWALQRNPAIWGLDANEYRPERHFGRRLCP---GIGFSLVLSKLAL 434 Query: 399 ILVTVIILQPLKMGP 443 + LK+GP Sbjct: 435 ANLVKRFNWRLKVGP 449 >At3g11110.1 68416.m01345 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile: PF00097 Zinc finger, C3HC4 type (RING finger) Length = 158 Score = 28.7 bits (61), Expect = 4.1 Identities = 19/58 (32%), Positives = 26/58 (44%), Gaps = 1/58 (1%) Frame = -2 Query: 350 GREPLQVRSAWFV*LPVFRSGRHQPRNDGEMGTVLDTVGFLDGEHFAVLLGCCDC-HC 180 G +P ++RS LPV R + + E + GF +GE VL C C HC Sbjct: 80 GLDPAEIRS-----LPVVLCRRERAEEEEEKECCICLGGFEEGEKMKVLPPCSHCYHC 132 >At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017 Length = 1340 Score = 28.7 bits (61), Expect = 4.1 Identities = 18/72 (25%), Positives = 31/72 (43%) Frame = +2 Query: 164 EEDKEYNGSRSSPEEPRNVRHQEILQYRERYPSRRRSLVDDARFETLVVKQTKQSVLEEA 343 EE++ R+ +E R +E + RER R R D E ++ ++ K+ E Sbjct: 357 EEERRREDQRARDKEREREREREHDRERERQRERERQRARDRERERILERREKERQGERE 416 Query: 344 RARANDSGLESD 379 R R ++ D Sbjct: 417 RERKRALEIKRD 428 >At1g16460.2 68414.m01969 mercaptopyruvate sulfurtransferase (MST2) (RDH2) identical to mercaptopyruvate sulfurtransferase GI:6009983 and thiosulfate sulfurtransferase GI:5817004 from [Arabidopsis thaliana]; contains PF|00581 Rhodanese-like domain Length = 342 Score = 28.7 bits (61), Expect = 4.1 Identities = 13/39 (33%), Positives = 25/39 (64%) Frame = -1 Query: 726 IEWDSSDLGRASLKVLNASFIIVDGFQNACEGSHSIAHM 610 ++W S+LG A +KVL+AS+ + +N + + +AH+ Sbjct: 48 VDWLHSNLGDADIKVLDASWYMAHEQRNPIQ-EYQVAHI 85 >At1g16460.1 68414.m01968 mercaptopyruvate sulfurtransferase (MST2) (RDH2) identical to mercaptopyruvate sulfurtransferase GI:6009983 and thiosulfate sulfurtransferase GI:5817004 from [Arabidopsis thaliana]; contains PF|00581 Rhodanese-like domain Length = 318 Score = 28.7 bits (61), Expect = 4.1 Identities = 13/39 (33%), Positives = 25/39 (64%) Frame = -1 Query: 726 IEWDSSDLGRASLKVLNASFIIVDGFQNACEGSHSIAHM 610 ++W S+LG A +KVL+AS+ + +N + + +AH+ Sbjct: 24 VDWLHSNLGDADIKVLDASWYMAHEQRNPIQ-EYQVAHI 61 >At3g58890.1 68416.m06563 syntaxin-related family protein contains a novel domain similar to F-box that is shared among other proteins in Arabidopsis; similar to proteins At3g59270,At1g56610, At3g54160, At1g47920 (syntaxin SYP81),, At5g41830, At3g44180, At1g48390 [Arabidopsis thaliana] Length = 293 Score = 28.3 bits (60), Expect = 5.5 Identities = 16/49 (32%), Positives = 22/49 (44%) Frame = -2 Query: 149 WDLKGLNVRFTARLASLCLRELLVSTTGLCLSACDSSCRITIAN*TLDC 3 W + +R L +L L L+ TG C +ACD R I + L C Sbjct: 179 WQAMPVLLRNCPHLETLVLEGLIHYVTGECGNACDCKSREGIVSSLLSC 227 >At2g14140.1 68415.m01575 hypothetical protein similar to At2g04970, At2g15200, At1g32830, At3g30450, At4g03990, At5g34895, At3g47270, At2g02200 Length = 847 Score = 28.3 bits (60), Expect = 5.5 Identities = 24/102 (23%), Positives = 41/102 (40%), Gaps = 5/102 (4%) Frame = +2 Query: 170 DKEYNGSRSSPEEPRNVRHQEILQYRERYPSRRRSL--VDDARFETLVVKQTKQSVLEEA 343 D+E G EE + +E ++YR + ++ + D E KQ ++ EE Sbjct: 473 DEEMEGEEEKQEEEGKEKEEEKVEYRGDEETEKQEIPKQGDEEMEGEEEKQEEEGKEEEE 532 Query: 344 R--ARANDSGLESDFI-QDGVHIGNGDNSPTVEDGTQQDETK 460 D G E I + G G+ E+G +++E K Sbjct: 533 EKVEYRGDEGTEKQEIPKQGDEEMEGEEEKQEEEGKEEEEEK 574 >At1g15420.1 68414.m01850 expressed protein Length = 278 Score = 28.3 bits (60), Expect = 5.5 Identities = 17/52 (32%), Positives = 26/52 (50%) Frame = +2 Query: 311 KQTKQSVLEEARARANDSGLESDFIQDGVHIGNGDNSPTVEDGTQQDETKNG 466 K K++ E DSGL+ D +DGV + + N PT+ D + + NG Sbjct: 31 KSKKRAEPEPDIPSTRDSGLDED--RDGVLVDDTLNEPTIGDKLESLDLLNG 80 >At5g54880.1 68418.m06836 DTW domain-containing protein contains Pfam domain, PF03942: DTW domain Length = 394 Score = 27.9 bits (59), Expect = 7.2 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = -3 Query: 427 GWRIITVTNMHSILDEIRFQARVIGSGAS 341 G + + VT + + DE F RVIGSG S Sbjct: 96 GLKNVGVTTVFDVHDEAEFLIRVIGSGCS 124 >At3g24080.1 68416.m03024 KRR1 family protein contains Pfam PF05178: Krr1 family Length = 638 Score = 27.9 bits (59), Expect = 7.2 Identities = 14/48 (29%), Positives = 26/48 (54%) Frame = +2 Query: 311 KQTKQSVLEEARARANDSGLESDFIQDGVHIGNGDNSPTVEDGTQQDE 454 K+T+++V + +A N+SG + DF++ G+ D E + DE Sbjct: 161 KETRKAVTDAWKAEGNESGEDDDFLRVVEKEGDDDVEVDEELAKKMDE 208 >At2g18890.1 68415.m02204 protein kinase family protein contains protein kinase domain, Pfam:PF00069 Length = 392 Score = 27.9 bits (59), Expect = 7.2 Identities = 12/37 (32%), Positives = 18/37 (48%) Frame = -2 Query: 302 VFRSGRHQPRNDGEMGTVLDTVGFLDGEHFAVLLGCC 192 + R GR R + E + T+G + + LLGCC Sbjct: 99 ITRGGRDDERREKEFLMEIGTIGHVSHPNVLSLLGCC 135 >At1g58170.1 68414.m06599 disease resistance-responsive protein-related / dirigent protein-related similar to dirigent protein [Thuja plicata] gi|6694699|gb|AAF25360; similar to pathogenesis-related protein [Pisum sativum] gi|4585273|gb|AAD25355 Length = 185 Score = 27.5 bits (58), Expect = 9.6 Identities = 17/43 (39%), Positives = 27/43 (62%) Frame = -3 Query: 454 FILLGPIFNGWRIITVTNMHSILDEIRFQARVIGSGASLFKYA 326 F +L +NG ITV +S+ D++R + VIG G+ LF++A Sbjct: 117 FAILDGKYNG-STITVLGRNSVFDKVR-EMPVIG-GSGLFRFA 156 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,488,035 Number of Sequences: 28952 Number of extensions: 315556 Number of successful extensions: 875 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 847 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 875 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1584903024 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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