Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30286
(715 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_37033| Best HMM Match : Annexin (HMM E-Value=0) 89 3e-18
SB_26806| Best HMM Match : Annexin (HMM E-Value=0) 71 7e-13
SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19) 64 1e-10
SB_47771| Best HMM Match : Annexin (HMM E-Value=0) 54 1e-07
SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25) 48 6e-06
SB_7681| Best HMM Match : Annexin (HMM E-Value=0) 43 3e-04
SB_15593| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.13
SB_49976| Best HMM Match : SEC-C (HMM E-Value=0.95) 31 0.70
SB_49312| Best HMM Match : Mucin (HMM E-Value=0.19) 31 1.2
SB_12472| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.1
SB_25942| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.5
SB_31195| Best HMM Match : PspA_IM30 (HMM E-Value=1) 28 6.5
SB_18204| Best HMM Match : Phage_integr_N (HMM E-Value=0.24) 28 6.5
SB_5137| Best HMM Match : Phage_integrase (HMM E-Value=0.11) 28 8.6
>SB_37033| Best HMM Match : Annexin (HMM E-Value=0)
Length = 287
Score = 89.0 bits (211), Expect = 3e-18
Identities = 55/134 (41%), Positives = 71/134 (52%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRT 435
LI +LKSE+ G E+ ++ALMTP + A L A+ G+GTDE +IE+L T +N I
Sbjct: 134 LIKDLKSEVGGYFEDTVIALMTPPAEYDATLLRKAIKGLGTDEAVLIEVLTTRTNDEIIA 193
Query: 436 ISAFYEQLYGKSLESDLKGDTSGHSRDCACRCAWPIAMKTRASMKAQLKPMLKHWAAAGE 615
I Y L+ + LE D+ GDTSG K L + AGE
Sbjct: 194 IRNAYNTLFSRDLEKDIAGDTSG-------------------KFKKFLISLCNALYKAGE 234
Query: 616 GQWGTDESIFNSIL 657
G+WGTDES FNSIL
Sbjct: 235 GRWGTDESKFNSIL 248
Score = 39.1 bits (87), Expect = 0.003
Identities = 19/70 (27%), Positives = 35/70 (50%)
Frame = +1
Query: 373 GTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHSRDCACRCAWPIAMK 552
G D +A++ +LC+ +N + IS Y+ ++G+ L DLK + G+ D P A
Sbjct: 101 GCDNKALMYLLCSRTNSQRQRISLEYKTMFGRDLIKDLKSEVGGYFEDTVIALMTPPAEY 160
Query: 553 TRASMKAQLK 582
++ +K
Sbjct: 161 DATLLRKAIK 170
Score = 34.3 bits (75), Expect = 0.100
Identities = 16/40 (40%), Positives = 24/40 (60%)
Frame = +2
Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
LRKA+KG GTDE +I+VL R + + I + T + +
Sbjct: 165 LRKAIKGLGTDEAVLIEVLTTRTNDEIIAIRNAYNTLFSR 204
Score = 29.5 bits (63), Expect = 2.8
Identities = 16/41 (39%), Positives = 23/41 (56%)
Frame = +2
Query: 131 TLRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
TL A G D KA++ +LC R QR I+ +KT +G+
Sbjct: 92 TLDIASNKPGCDNKALMYLLCSRTNSQRQRISLEYKTMFGR 132
>SB_26806| Best HMM Match : Annexin (HMM E-Value=0)
Length = 829
Score = 71.3 bits (167), Expect = 7e-13
Identities = 41/134 (30%), Positives = 67/134 (50%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRT 435
L+ +L ++ ++ + +L+ P + L+ A+ G+GT E +IEILCT SN I+
Sbjct: 490 LVEQLDGKVAIDVHEAVTSLLLPATKADVRSLYYAMKGLGTKEAQLIEILCTRSNAEIQE 549
Query: 436 ISAFYEQLYGKSLESDLKGDTSGHSRDCACRCAWPIAMKTRASMKAQLKPMLKHWAAAGE 615
I Y + YG+SL D+K +TSG + ++ K + +AG
Sbjct: 550 IKQEYNKKYGRSLTDDMKSETSGDFENLLETLLKGSRDESTDVNKELVTKDAAALVSAGV 609
Query: 616 GQWGTDESIFNSIL 657
+WGTDE F +IL
Sbjct: 610 ARWGTDEDQFITIL 623
Score = 37.5 bits (83), Expect = 0.011
Identities = 16/42 (38%), Positives = 25/42 (59%)
Frame = +2
Query: 131 TLRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGKA 256
+L AMKG GT E +I++LC R + EI + + YG++
Sbjct: 520 SLYYAMKGLGTKEAQLIEILCTRSNAEIQEIKQEYNKKYGRS 561
Score = 31.5 bits (68), Expect = 0.70
Identities = 21/61 (34%), Positives = 35/61 (57%), Gaps = 3/61 (4%)
Frame = +1
Query: 343 KELHDAVSGI---GTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHSR 513
++LH+AV+ + G + +A + + N I +I Y++ YG SLE D+K +TSG
Sbjct: 60 EDLHEAVAAMLLPGNEADART-LYFAMKN--IDSIKEEYQKKYGCSLEDDVKEETSGDFE 116
Query: 514 D 516
D
Sbjct: 117 D 117
Score = 28.3 bits (60), Expect = 6.5
Identities = 20/53 (37%), Positives = 25/53 (47%)
Frame = +3
Query: 543 RDENQGIDEGSAKADAEALGRRW*RSMGNRRINLQLHPYHRSYQQLRQIFAEY 701
RDE+ I+E A DAEAL + G RSY QL+ +F EY
Sbjct: 160 RDESDEINEDLAHKDAEALIEAGIKKWGTDEDCFIEIFTTRSYPQLQAMFPEY 212
>SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19)
Length = 146
Score = 63.7 bits (148), Expect = 1e-10
Identities = 31/59 (52%), Positives = 42/59 (71%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIR 432
L+++LKSEL+GNLE ++A+M P + AK L + G GTDEE +I+ILCT SN G R
Sbjct: 34 LMNDLKSELSGNLEECLLAMMEPSVLYDAKCLRRGMRGAGTDEETLIDILCTRSNQGNR 92
Score = 37.5 bits (83), Expect = 0.011
Identities = 19/48 (39%), Positives = 27/48 (56%)
Frame = +1
Query: 376 TDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHSRDC 519
T+E +I IL SN I Y+ +YGK L +DLK + SG+ +C
Sbjct: 2 TNEAELIGILANRSNAQRVEIRKRYKTMYGKDLMNDLKSELSGNLEEC 49
Score = 35.1 bits (77), Expect = 0.057
Identities = 13/22 (59%), Positives = 18/22 (81%)
Frame = +2
Query: 134 LRKAMKGFGTDEKAIIDVLCRR 199
LR+ M+G GTDE+ +ID+LC R
Sbjct: 65 LRRGMRGAGTDEETLIDILCTR 86
Score = 32.7 bits (71), Expect = 0.30
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +2
Query: 161 TDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
T+E +I +L R QR+EI + +KT YGK
Sbjct: 2 TNEAELIGILANRSNAQRVEIRKRYKTMYGK 32
Score = 31.5 bits (68), Expect = 0.70
Identities = 18/62 (29%), Positives = 26/62 (41%)
Frame = +3
Query: 516 LCVSLCMANRDENQGIDEGSAKADAEALGRRW*RSMGNRRINLQLHPYHRSYQQLRQIFA 695
LC NRD N +D A +A+ L + + G + RS+ QL+ F
Sbjct: 83 LCTRSNQGNRDTNMTVDMAKATKEAQDLYKAGEKKWGTDESRFNVVLASRSFPQLQATFN 142
Query: 696 EY 701
EY
Sbjct: 143 EY 144
>SB_47771| Best HMM Match : Annexin (HMM E-Value=0)
Length = 529
Score = 54.0 bits (124), Expect = 1e-07
Identities = 41/122 (33%), Positives = 59/122 (48%), Gaps = 16/122 (13%)
Frame = +1
Query: 340 AKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHSRDC 519
A++L A+ G G+DEEAIIE+L + + I+ ++Q Y K L ++LK + SG D
Sbjct: 308 AEKLKKAMKGFGSDEEAIIEVLQSCNLEQRLAIAQSFKQQYDKDLVNELKSELSGKFEDA 367
Query: 520 ACRCAWP----IAMKTRASMKA------------QLKPMLKHWAAAGEGQWGTDESIFNS 651
P A +M+A Q K+ AGE +WGTDES+F
Sbjct: 368 IVALLLPPEQLDANALHGAMQAKRVSADEIVNEDQAYEDAKNLYEAGEKKWGTDESVFTK 427
Query: 652 IL 657
IL
Sbjct: 428 IL 429
Score = 50.8 bits (116), Expect = 1e-06
Identities = 24/40 (60%), Positives = 31/40 (77%)
Frame = +2
Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
L+KAMKGFG+DE+AII+VL + QRL IA++FK Y K
Sbjct: 311 LKKAMKGFGSDEEAIIEVLQSCNLEQRLAIAQSFKQQYDK 350
Score = 42.3 bits (95), Expect = 4e-04
Identities = 18/57 (31%), Positives = 32/57 (56%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYG 426
L+ +L ++ ++ + +L+ P + L+ A+ G+GT E +IE LCT SN G
Sbjct: 93 LVEQLDGKVADDVHEAVTSLLLPATQADVRSLYYAMKGLGTKEAQLIETLCTRSNTG 149
Score = 38.7 bits (86), Expect = 0.005
Identities = 19/47 (40%), Positives = 27/47 (57%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAII 396
L++ELKSEL+G E+ IVAL+ P A LH A+ + I+
Sbjct: 352 LVNELKSELSGKFEDAIVALLLPPEQLDANALHGAMQAKRVSADEIV 398
Score = 27.9 bits (59), Expect = 8.6
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +2
Query: 131 TLRKAMKGFGTDEKAIIDVLCRR 199
+L AMKG GT E +I+ LC R
Sbjct: 123 SLYYAMKGLGTKEAQLIETLCTR 145
>SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25)
Length = 803
Score = 48.4 bits (110), Expect = 6e-06
Identities = 21/57 (36%), Positives = 36/57 (63%)
Frame = +1
Query: 250 QGLISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSN 420
Q ++ L+ L ++++ + + + F AK LHDA+ G+GTDE+ +IEI+CT N
Sbjct: 396 QDILEALQPHLGDDVKDPVESFFSSSSAFDAKCLHDAIKGLGTDEDLLIEIICTRKN 452
Score = 46.8 bits (106), Expect = 2e-05
Identities = 26/59 (44%), Positives = 33/59 (55%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIR 432
L ELKS L + + +L AK L++A G GTDE+A+I ILCT SN G R
Sbjct: 53 LNEELKSVLDEEIMKALESLAEEAAMKDAKTLYEATKGFGTDEDALISILCTRSNKGQR 111
Score = 35.9 bits (79), Expect = 0.033
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +2
Query: 131 TLRKAMKGFGTDEKAIIDVLCRR 199
TL +A KGFGTDE A+I +LC R
Sbjct: 83 TLYEATKGFGTDEDALISILCTR 105
Score = 30.3 bits (65), Expect = 1.6
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = +2
Query: 134 LRKAMKGFGTDEKAIIDVLCRR 199
L A+KG GTDE +I+++C R
Sbjct: 429 LHDAIKGLGTDEDLLIEIICTR 450
>SB_7681| Best HMM Match : Annexin (HMM E-Value=0)
Length = 426
Score = 42.7 bits (96), Expect = 3e-04
Identities = 22/83 (26%), Positives = 45/83 (54%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRT 435
+++ + E++G+L+ + P +F A L ++ G GTD+ +I ++ + S +
Sbjct: 63 IMNSIDREMSGDLKAGLQCARNPAEYF-ADRLWKSMKGAGTDDSLLIRVVVSRSEVDLVE 121
Query: 436 ISAFYEQLYGKSLESDLKGDTSG 504
I A + Q Y K++ ++GD SG
Sbjct: 122 IKASFLQKYHKTVYKMIEGDCSG 144
Score = 33.1 bits (72), Expect = 0.23
Identities = 17/40 (42%), Positives = 23/40 (57%)
Frame = +2
Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
L K+MKG GTD+ +I V+ R V +EI +F Y K
Sbjct: 93 LWKSMKGAGTDDSLLIRVVVSRSEVDLVEIKASFLQKYHK 132
Score = 30.3 bits (65), Expect = 1.6
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = +1
Query: 607 AGEGQWGTDESIFNSIL 657
AGE +WGTDES FN +L
Sbjct: 24 AGEKKWGTDESRFNVVL 40
>SB_15593| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1593
Score = 33.9 bits (74), Expect = 0.13
Identities = 22/72 (30%), Positives = 41/72 (56%), Gaps = 4/72 (5%)
Frame = +1
Query: 304 IVALMTPLPHFYAK-ELHDAVSGIGTDEEA---IIEILCTLSNYGIRTISAFYEQLYGKS 471
+V + +F K E+HDA+ G T+ ++ +++ L ++YG + S + +L G S
Sbjct: 537 VVTVFNMTSNFTTKSEVHDAIDGAVTENDSYRKVLDFLKEWASYGNKEYSEAWIRLDGIS 596
Query: 472 LESDLKGDTSGH 507
+E+ LKG +S H
Sbjct: 597 VEA-LKGKSSWH 607
>SB_49976| Best HMM Match : SEC-C (HMM E-Value=0.95)
Length = 1037
Score = 31.5 bits (68), Expect = 0.70
Identities = 27/114 (23%), Positives = 49/114 (42%), Gaps = 5/114 (4%)
Frame = +1
Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILC---TLSNYG 426
L+ +K+E TGN E + + +PHF++ + + S + E + +S G
Sbjct: 692 LLQFIKAERTGNWELHLASTAAMIPHFFSMDRPNYSSWKSKNPRVFEEFMAGNHAVSRSG 751
Query: 427 IRTISAFYEQLYGKSLESD--LKGDTSGHSRDCACRCAWPIAMKTRASMKAQLK 582
+ + +S+ +D KG G S++ A W + RAS+ LK
Sbjct: 752 QPFSQVWTDMALEQSVNADSKSKGGIVGISQNPAALQRWFLTSHERASVTTALK 805
>SB_49312| Best HMM Match : Mucin (HMM E-Value=0.19)
Length = 925
Score = 30.7 bits (66), Expect = 1.2
Identities = 19/70 (27%), Positives = 28/70 (40%)
Frame = +2
Query: 263 ANSRVNSPATWKMSSSH**LPCPTSTLRSSTMLSQELEPTKKPSSRSCARFPTMVSVPYP 442
A N P T S +P T+ + ++ L+PTK S + PT+ +P
Sbjct: 206 AKGTYNFPLTKSTKSQPATIPVSTTKFPTEAQTTEYLQPTKTSLSSTAPPHPTVYRLPST 265
Query: 443 HFTNNCTARA 472
NN T A
Sbjct: 266 ESINNFTDTA 275
>SB_12472| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 175
Score = 29.9 bits (64), Expect = 2.1
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +3
Query: 327 APLLR*GAPRCCLRNWNRRRSHHRDP 404
+P L PRCC +N N R H DP
Sbjct: 138 SPFLYEPGPRCCNQNMNNRSKCHADP 163
>SB_25942| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1062
Score = 28.3 bits (60), Expect = 6.5
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 337 YAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLE 477
Y K + + VSG GTD + + I T S ISA + K++E
Sbjct: 493 YVKYMRNKVSGAGTDPDEKVFISFTASEMTSSMISAQLNSFWQKAVE 539
>SB_31195| Best HMM Match : PspA_IM30 (HMM E-Value=1)
Length = 345
Score = 28.3 bits (60), Expect = 6.5
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 337 YAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLE 477
Y K + + VSG GTD + + I T S ISA + K++E
Sbjct: 47 YVKYMRNKVSGAGTDPDEKVFISFTASEMTSSMISAQLNSFWQKAVE 93
>SB_18204| Best HMM Match : Phage_integr_N (HMM E-Value=0.24)
Length = 285
Score = 28.3 bits (60), Expect = 6.5
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +1
Query: 337 YAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLE 477
Y K + + VSG GTD + + I T S ISA + K++E
Sbjct: 229 YVKYMRNKVSGAGTDPDEKVFISFTASEMTSSMISAQLNSFWQKAVE 275
>SB_5137| Best HMM Match : Phage_integrase (HMM E-Value=0.11)
Length = 834
Score = 27.9 bits (59), Expect = 8.6
Identities = 16/46 (34%), Positives = 24/46 (52%)
Frame = +1
Query: 337 YAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSL 474
Y K + + VSG GTD + + I T+S ISA L+ K++
Sbjct: 582 YVKYMRNKVSGAGTDPDEKVFISFTVSEMTSFMISAQLNSLWQKAV 627
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,907,480
Number of Sequences: 59808
Number of extensions: 414884
Number of successful extensions: 1176
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1038
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1171
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1889780269
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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