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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30286
         (715 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g68090.1 68414.m07778 annexin 5 (ANN5) identical to calcium-b...    51   7e-07
At2g38760.1 68415.m04759 annexin 3 (ANN3) nearly identical to an...    50   1e-06
At5g12380.1 68418.m01456 annexin, putative similar to annexin [F...    43   2e-04
At5g10220.1 68418.m01185 annexin 6 (ANN6) nearly identical to ca...    41   0.001
At5g10230.1 68418.m01187 annexin 7 (ANN7) nearly identical to ca...    39   0.003
At5g65020.1 68418.m08179 annexin 2 (ANN2) identical to annexin (...    36   0.020
At1g35720.1 68414.m04440 annexin 1 (ANN1) identical to annexin (...    36   0.035
At2g40960.1 68415.m05058 expressed protein                             27   9.3  
At1g51560.1 68414.m05803 expressed protein                             27   9.3  

>At1g68090.1 68414.m07778 annexin 5 (ANN5) identical to
           calcium-binding protein annexin 5 [Arabidopsis thaliana]
           GI:12667520
          Length = 316

 Score = 51.2 bits (117), Expect = 7e-07
 Identities = 25/85 (29%), Positives = 49/85 (57%), Gaps = 3/85 (3%)
 Frame = +1

Query: 268 LKSELTGNLENVIVALMTPLPH---FYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTI 438
           ++ E  GN E+V++ ++    +   ++AK L  ++ G+GTD+ A+I I+ T +   ++ I
Sbjct: 221 IRDETRGNFEHVLLTILQCAENSCFYFAKALRKSMKGLGTDDTALIRIVVTRAEVDMQFI 280

Query: 439 SAFYEQLYGKSLESDLKGDTSGHSR 513
              Y + Y K+L + +  DT+ H R
Sbjct: 281 ITEYRKRYKKTLYNAVHSDTTSHYR 305



 Score = 33.9 bits (74), Expect = 0.11
 Identities = 16/40 (40%), Positives = 23/40 (57%)
 Frame = +2

Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
           LRK+MKG GTD+ A+I ++  R  V    I   ++  Y K
Sbjct: 251 LRKSMKGLGTDDTALIRIVVTRAEVDMQFIITEYRKRYKK 290



 Score = 32.7 bits (71), Expect = 0.25
 Identities = 12/39 (30%), Positives = 24/39 (61%)
 Frame = +2

Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYG 250
           L+++++G  TD KAI +++C R   Q  +I + +   +G
Sbjct: 92  LKRSLRGAVTDHKAIAEIICTRSGSQLRQIKQVYSNTFG 130


>At2g38760.1 68415.m04759 annexin 3 (ANN3) nearly identical to
           annexin (AnnAt3) [Arabidopsis thaliana] GI:6503082;
           contains Pfam profile PF00191: Annexin
          Length = 321

 Score = 50.4 bits (115), Expect = 1e-06
 Identities = 24/81 (29%), Positives = 44/81 (54%)
 Frame = +1

Query: 274 SELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYE 453
           ++L   L+  I  + TP  HF AK + D++ G GTDE+++   + T +   +  +   Y 
Sbjct: 232 ADLRSLLKVAIFCIDTPEKHF-AKVVRDSIEGFGTDEDSLTRAIVTRAEIDLMKVRGEYF 290

Query: 454 QLYGKSLESDLKGDTSGHSRD 516
            +Y  S+++ + GD SG  +D
Sbjct: 291 NMYNTSMDNAITGDISGDYKD 311



 Score = 47.6 bits (108), Expect = 8e-06
 Identities = 23/41 (56%), Positives = 32/41 (78%)
 Frame = +2

Query: 131 TLRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
           TL++A++G+GTDEKAII VL +R   QR +I E+F+  YGK
Sbjct: 19  TLKQAIRGWGTDEKAIIRVLGQRDQSQRRKIRESFREIYGK 59



 Score = 39.5 bits (88), Expect = 0.002
 Identities = 22/61 (36%), Positives = 31/61 (50%)
 Frame = +1

Query: 322 PLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTS 501
           P P   ++ L  A+ G GTDE+AII +L        R I   + ++YGK L   L  + S
Sbjct: 11  PSPAQDSETLKQAIRGWGTDEKAIIRVLGQRDQSQRRKIRESFREIYGKDLIDVLSSELS 70

Query: 502 G 504
           G
Sbjct: 71  G 71


>At5g12380.1 68418.m01456 annexin, putative similar to annexin
           [Fragaria x ananassa] GI:6010777, annexin p33 [Zea mays]
           GI:6272285; contains Pfam profile PF00191: Annexin
          Length = 316

 Score = 43.2 bits (97), Expect = 2e-04
 Identities = 30/88 (34%), Positives = 40/88 (45%)
 Frame = +1

Query: 250 QGLISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGI 429
           + LI +LKSEL+GN E  I   +   P   A   + A+     D + ++EI C  S   +
Sbjct: 58  EDLIHQLKSELSGNFERAICLWVLDPPERDALLANLALQKPIPDYKVLVEIACMRSPEDM 117

Query: 430 RTISAFYEQLYGKSLESDLKGDTSGHSR 513
                 Y  LY  SLE DL   T G  R
Sbjct: 118 LAARRAYRCLYKHSLEEDLASRTIGDIR 145



 Score = 41.5 bits (93), Expect = 5e-04
 Identities = 22/83 (26%), Positives = 43/83 (51%)
 Frame = +1

Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRT 435
           L++   +E    L   I  +  P   +YAK L ++++ +GTDE+A+  ++ T +   +  
Sbjct: 219 LLNHPTNEYLSALRAAIRCIKNPT-RYYAKVLRNSINTVGTDEDALNRVIVTRAEKDLTN 277

Query: 436 ISAFYEQLYGKSLESDLKGDTSG 504
           I+  Y +    SL+  +  +TSG
Sbjct: 278 ITGLYFKRNNVSLDQAIAKETSG 300



 Score = 39.1 bits (87), Expect = 0.003
 Identities = 28/95 (29%), Positives = 45/95 (47%), Gaps = 4/95 (4%)
 Frame = +1

Query: 307 VALMTPLPHFY----AKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSL 474
           +A +   PHF     A+ +  A  G GT+E AII IL   + +  + I   Y+++Y + L
Sbjct: 1   MATIVSPPHFSPVEDAENIKAACQGWGTNENAIISILGHRNLFQRKLIRQAYQEIYHEDL 60

Query: 475 ESDLKGDTSGHSRDCACRCAWPIAMKTRASMKAQL 579
              LK + SG+       C W +    R ++ A L
Sbjct: 61  IHQLKSELSGNFERAI--CLWVLDPPERDALLANL 93



 Score = 36.3 bits (80), Expect = 0.020
 Identities = 17/46 (36%), Positives = 24/46 (52%)
 Frame = +1

Query: 349 LHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDL 486
           LHD + G   D E  I +L T S+  +  I   Y+ +YG S+  DL
Sbjct: 174 LHDEILGKAVDHEETIRVLSTRSSMQLSAIFNRYKDIYGTSITKDL 219



 Score = 32.3 bits (70), Expect = 0.33
 Identities = 14/38 (36%), Positives = 24/38 (63%)
 Frame = +2

Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNY 247
           ++ A +G+GT+E AII +L  R + QR  I + ++  Y
Sbjct: 19  IKAACQGWGTNENAIISILGHRNLFQRKLIRQAYQEIY 56


>At5g10220.1 68418.m01185 annexin 6 (ANN6) nearly identical to
           calcium-binding protein annexin 6 [Arabidopsis thaliana]
           GI:12667518
          Length = 318

 Score = 40.7 bits (91), Expect = 0.001
 Identities = 24/61 (39%), Positives = 31/61 (50%)
 Frame = +1

Query: 322 PLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTS 501
           PLP   +++LH A  G GT+E  II IL   +      I A Y   Y K L  +L G+ S
Sbjct: 11  PLPEEDSEQLHKAFKGWGTNEGMIISILAHRNATQRSFIRAVYAANYNKDLLKELDGELS 70

Query: 502 G 504
           G
Sbjct: 71  G 71



 Score = 38.3 bits (85), Expect = 0.005
 Identities = 18/40 (45%), Positives = 22/40 (55%)
 Frame = +2

Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
           L KA KG+GT+E  II +L  R   QR  I   +  NY K
Sbjct: 20  LHKAFKGWGTNEGMIISILAHRNATQRSFIRAVYAANYNK 59



 Score = 33.5 bits (73), Expect = 0.14
 Identities = 23/86 (26%), Positives = 37/86 (43%)
 Frame = +1

Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRT 435
           L+ EL  EL+G+ E V++          A   +++      +   ++EI CT  +     
Sbjct: 61  LLKELDGELSGDFERVVMLWTLDPTERDAYLANESTKLFTKNIWVLVEIACTRPSLEFFK 120

Query: 436 ISAFYEQLYGKSLESDLKGDTSGHSR 513
               Y   Y  SLE D+   TSG+ R
Sbjct: 121 TKQAYHVRYKTSLEEDVAYHTSGNIR 146


>At5g10230.1 68418.m01187 annexin 7 (ANN7) nearly identical to
           calcium-binding protein annexin 7 [Arabidopsis thaliana]
           GI:12667522
          Length = 316

 Score = 39.1 bits (87), Expect = 0.003
 Identities = 18/40 (45%), Positives = 23/40 (57%)
 Frame = +2

Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
           L KA KG+GT+E+ II +L  R   QR  I   +  NY K
Sbjct: 20  LYKAFKGWGTNERMIISILAHRNATQRSFIRAVYAANYNK 59



 Score = 37.5 bits (83), Expect = 0.009
 Identities = 24/66 (36%), Positives = 31/66 (46%)
 Frame = +1

Query: 307 VALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDL 486
           V    PLP   A++L+ A  G GT+E  II IL   +      I A Y   Y K L  +L
Sbjct: 6   VPATVPLPEEDAEQLYKAFKGWGTNERMIISILAHRNATQRSFIRAVYAANYNKDLLKEL 65

Query: 487 KGDTSG 504
             + SG
Sbjct: 66  DRELSG 71



 Score = 34.3 bits (75), Expect = 0.081
 Identities = 23/86 (26%), Positives = 36/86 (41%)
 Frame = +1

Query: 256 LISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRT 435
           L+ EL  EL+G+ E  ++          A    ++      +   ++EI CT S   +  
Sbjct: 61  LLKELDRELSGDFERAVMLWTFEPAERDAYLAKESTKMFTKNNWVLVEIACTRSALELFN 120

Query: 436 ISAFYEQLYGKSLESDLKGDTSGHSR 513
               Y+  Y  SLE D+   TSG  R
Sbjct: 121 AKQAYQARYKTSLEEDVAYHTSGDIR 146


>At5g65020.1 68418.m08179 annexin 2 (ANN2) identical to annexin
           (AnnAt2) [Arabidopsis thaliana] GI:4959108
          Length = 317

 Score = 36.3 bits (80), Expect = 0.020
 Identities = 24/62 (38%), Positives = 35/62 (56%), Gaps = 4/62 (6%)
 Frame = +1

Query: 322 PLPHFYAKELHDAVSGIGTDEEAIIEILC---TLSNYGIRTI-SAFYEQLYGKSLESDLK 489
           PLP   A++LH A SG GT+E+ II IL          IR++ +A Y +   K+L+ +L 
Sbjct: 11  PLPEDDAEQLHKAFSGWGTNEKLIISILAHRNAAQRSLIRSVYAATYNEDLLKALDKELS 70

Query: 490 GD 495
            D
Sbjct: 71  SD 72



 Score = 35.1 bits (77), Expect = 0.047
 Identities = 21/88 (23%), Positives = 37/88 (42%)
 Frame = +1

Query: 250 QGLISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGI 429
           + L+  L  EL+ + E  ++      P   A    ++      +   ++EI CT     +
Sbjct: 59  EDLLKALDKELSSDFERAVMLWTLDPPERDAYLAKESTKMFTKNNWVLVEIACTRPALEL 118

Query: 430 RTISAFYEQLYGKSLESDLKGDTSGHSR 513
             +   Y+  Y KS+E D+   TSG  R
Sbjct: 119 IKVKQAYQARYKKSIEEDVAQHTSGDLR 146



 Score = 28.7 bits (61), Expect = 4.0
 Identities = 16/53 (30%), Positives = 25/53 (47%)
 Frame = +1

Query: 340 AKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDT 498
           AK LH+ VS     ++  I IL T S   +      Y   YG ++  +LK ++
Sbjct: 172 AKILHEKVSEKSYSDDDFIRILTTRSKAQLGATLNHYNNEYGNAINKNLKEES 224


>At1g35720.1 68414.m04440 annexin 1 (ANN1) identical to annexin
           (AnnAt1) [Arabidopsis thaliana] GI:4959106
          Length = 317

 Score = 35.5 bits (78), Expect = 0.035
 Identities = 16/40 (40%), Positives = 23/40 (57%)
 Frame = +2

Query: 134 LRKAMKGFGTDEKAIIDVLCRRGIVQRLEIAETFKTNYGK 253
           LR A +G+GT+E  II +L  R   QR  I + +   YG+
Sbjct: 20  LRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGE 59



 Score = 35.1 bits (77), Expect = 0.047
 Identities = 22/62 (35%), Positives = 31/62 (50%), Gaps = 4/62 (6%)
 Frame = +1

Query: 322 PLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYG----KSLESDLK 489
           P P   A++L  A  G GT+E+ II IL   S    + I   Y + YG    K+L+ +L 
Sbjct: 11  PAPSDDAEQLRTAFEGWGTNEDLIISILAHRSAEQRKVIRQAYHETYGEDLLKTLDKELS 70

Query: 490 GD 495
            D
Sbjct: 71  ND 72



 Score = 32.7 bits (71), Expect = 0.25
 Identities = 21/88 (23%), Positives = 38/88 (43%)
 Frame = +1

Query: 250 QGLISELKSELTGNLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGI 429
           + L+  L  EL+ + E  I+          A   ++A     +  + ++E+ CT ++  +
Sbjct: 59  EDLLKTLDKELSNDFERAILLWTLEPGERDALLANEATKRWTSSNQVLMEVACTRTSTQL 118

Query: 430 RTISAFYEQLYGKSLESDLKGDTSGHSR 513
                 Y   Y KSLE D+   T+G  R
Sbjct: 119 LHARQAYHARYKKSLEEDVAHHTTGDFR 146


>At2g40960.1 68415.m05058 expressed protein
          Length = 351

 Score = 27.5 bits (58), Expect = 9.3
 Identities = 10/26 (38%), Positives = 17/26 (65%)
 Frame = +3

Query: 531 CMANRDENQGIDEGSAKADAEALGRR 608
           C ++RDENQ +  G+ +AD   + R+
Sbjct: 191 CSSSRDENQVLKNGNIEADKNNISRK 216


>At1g51560.1 68414.m05803 expressed protein
          Length = 392

 Score = 27.5 bits (58), Expect = 9.3
 Identities = 16/34 (47%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = -1

Query: 547 SRLAMHNDTHNLLSVPTCRLLSPIPG-SCRTVVR 449
           S LA+H  T N+L+ P C L+  IPG SC +  R
Sbjct: 198 SPLAIH--TRNILAEPRCTLVVQIPGWSCLSNAR 229


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,258,670
Number of Sequences: 28952
Number of extensions: 279524
Number of successful extensions: 793
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 792
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1545769616
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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