BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30283 (703 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 111 9e-26 SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 111 1e-25 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 2.0 SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos... 27 2.6 SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subuni... 26 6.0 SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|... 26 6.0 SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo... 25 7.9 SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizos... 25 7.9 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 111 bits (267), Expect = 9e-26 Identities = 49/85 (57%), Positives = 62/85 (72%) Frame = +2 Query: 2 MRNRRRIQRKGPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQS 181 +RNRR +QR+GPL++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+S Sbjct: 196 LRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKS 255 Query: 182 AFGRLDPLFGSWKTPSKQKKNFNLP 256 AFG LD +FGS ++ KKN+ LP Sbjct: 256 AFGLLDSVFGSTTEAAQLKKNYFLP 280 Score = 50.4 bits (115), Expect = 2e-07 Identities = 30/73 (41%), Positives = 44/73 (60%), Gaps = 2/73 (2%) Frame = +1 Query: 265 MANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAIL 438 ++N D+TRL+ SDEI+ +++A RV RA +K NPL N + +LNPYA KA + Sbjct: 284 ISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLARLNPYAKAY--KANV 341 Query: 439 ELRRRKNLKALAD 477 +L K KA + Sbjct: 342 KLNTGKTPKAAGE 354 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 111 bits (266), Expect = 1e-25 Identities = 49/85 (57%), Positives = 62/85 (72%) Frame = +2 Query: 2 MRNRRRIQRKGPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQS 181 +RNRR +QR+GPL++FN+D G+ +AFRNIPGVE APGGHLGRFVIWT+S Sbjct: 196 LRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKS 255 Query: 182 AFGRLDPLFGSWKTPSKQKKNFNLP 256 AFG LD +FGS ++ KKN+ LP Sbjct: 256 AFGLLDSVFGSTTEVAQLKKNYFLP 280 Score = 50.0 bits (114), Expect = 3e-07 Identities = 29/73 (39%), Positives = 44/73 (60%), Gaps = 2/73 (2%) Frame = +1 Query: 265 MANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAIL 438 ++N D+TRL+ SDEI+ +++A RV RA +K NPL N + +LNPYA KA + Sbjct: 284 ISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLSRLNPYAKAY--KANV 341 Query: 439 ELRRRKNLKALAD 477 ++ K KA + Sbjct: 342 KINSEKTPKAAGE 354 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 27.5 bits (58), Expect = 2.0 Identities = 21/75 (28%), Positives = 38/75 (50%) Frame = +1 Query: 178 VRIRQA*PLIRVMEDTIKTKEELQPAQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATR 357 + RQ+ L V E+T + + L +QP + +L SD ++L+ N ++ +A Sbjct: 441 IEARQSEKLNEVQEETQRLSQLLISSQPALTEVKHLKLCLSDSQEELLQL-NAKLEKANI 499 Query: 358 KLNPLTNNKAMLKLN 402 ++ L N A LKL+ Sbjct: 500 VIDEL--NSAKLKLS 512 >SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 27.1 bits (57), Expect = 2.6 Identities = 13/24 (54%), Positives = 15/24 (62%), Gaps = 3/24 (12%) Frame = +2 Query: 209 GSWKTPSKQKKNFN---LPNRRWP 271 G K PSK+ K F LPNR+WP Sbjct: 14 GMLKDPSKKYKPFKGIQLPNRQWP 37 >SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subunit 8|Schizosaccharomyces pombe|chr 3|||Manual Length = 230 Score = 25.8 bits (54), Expect = 6.0 Identities = 11/48 (22%), Positives = 25/48 (52%) Frame = +1 Query: 229 KTKEELQPAQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPL 372 + K++ Q ++ + + ++ RL + E+R + NK+ + KL L Sbjct: 173 RLKQKKQRSEFRASKREMERLERDQELRNKIEERNKKELETIEKLREL 220 >SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 25.8 bits (54), Expect = 6.0 Identities = 19/59 (32%), Positives = 27/59 (45%) Frame = +1 Query: 229 KTKEELQPAQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNP 405 K K L Q K LT+ S E + VL +R T+K LTN+ ++K +P Sbjct: 730 KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSP 786 >SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 508 Score = 25.4 bits (53), Expect = 7.9 Identities = 10/39 (25%), Positives = 20/39 (51%) Frame = -2 Query: 411 RVRIEFQHRLVISEWVQFACSTDHAFVGSTEDLPDLIRL 295 + ++ F H L + +WV+ + +G + PD +RL Sbjct: 171 KYKVLFYHSLNLRDWVKLSEFGSAGVLGYQYECPDFVRL 209 >SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/39 (33%), Positives = 17/39 (43%), Gaps = 2/39 (5%) Frame = +2 Query: 215 WKTPSKQKKNFNL--PNRRWPTLTSHVFSSLMRSGRSSV 325 WK P K FNL R P S + SL + R ++ Sbjct: 444 WKVPHTSDKRFNLYVTQRNDPNTKSQLIESLPMNSRDTI 482 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,340,499 Number of Sequences: 5004 Number of extensions: 43916 Number of successful extensions: 128 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 126 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 325165428 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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