SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30283
         (703 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo...   111   9e-26
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo...   111   1e-25
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po...    27   2.0  
SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos...    27   2.6  
SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subuni...    26   6.0  
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    26   6.0  
SPAC8E11.01c ||SPAC959.01|beta-fructofuranosidase|Schizosaccharo...    25   7.9  
SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8 |Schizos...    25   7.9  

>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  111 bits (267), Expect = 9e-26
 Identities = 49/85 (57%), Positives = 62/85 (72%)
 Frame = +2

Query: 2   MRNRRRIQRKGPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQS 181
           +RNRR +QR+GPL++FN+D G+ +AFRNIPGVE            APGGHLGRFVIWT+S
Sbjct: 196 LRNRRHVQRRGPLVVFNEDAGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKS 255

Query: 182 AFGRLDPLFGSWKTPSKQKKNFNLP 256
           AFG LD +FGS    ++ KKN+ LP
Sbjct: 256 AFGLLDSVFGSTTEAAQLKKNYFLP 280



 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 30/73 (41%), Positives = 44/73 (60%), Gaps = 2/73 (2%)
 Frame = +1

Query: 265 MANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAIL 438
           ++N D+TRL+ SDEI+ +++A    RV RA  +K NPL N   + +LNPYA     KA +
Sbjct: 284 ISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLARLNPYAKAY--KANV 341

Query: 439 ELRRRKNLKALAD 477
           +L   K  KA  +
Sbjct: 342 KLNTGKTPKAAGE 354


>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
           L2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 363

 Score =  111 bits (266), Expect = 1e-25
 Identities = 49/85 (57%), Positives = 62/85 (72%)
 Frame = +2

Query: 2   MRNRRRIQRKGPLIIFNKDQGLTRAFRNIPGVEXXXXXXXXXXXXAPGGHLGRFVIWTQS 181
           +RNRR +QR+GPL++FN+D G+ +AFRNIPGVE            APGGHLGRFVIWT+S
Sbjct: 196 LRNRRHVQRRGPLVVFNEDTGIVKAFRNIPGVEIVNVRRLNLLQLAPGGHLGRFVIWTKS 255

Query: 182 AFGRLDPLFGSWKTPSKQKKNFNLP 256
           AFG LD +FGS    ++ KKN+ LP
Sbjct: 256 AFGLLDSVFGSTTEVAQLKKNYFLP 280



 Score = 50.0 bits (114), Expect = 3e-07
 Identities = 29/73 (39%), Positives = 44/73 (60%), Gaps = 2/73 (2%)
 Frame = +1

Query: 265 MANTDLTRLLKSDEIRKVLRAPN-KRVIRA-TRKLNPLTNNKAMLKLNPYAAVLKRKAIL 438
           ++N D+TRL+ SDEI+ +++A    RV RA  +K NPL N   + +LNPYA     KA +
Sbjct: 284 ISNADVTRLINSDEIQSIVKAAGPSRVKRAHVQKKNPLKNKAVLSRLNPYAKAY--KANV 341

Query: 439 ELRRRKNLKALAD 477
           ++   K  KA  +
Sbjct: 342 KINSEKTPKAAGE 354


>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 670

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 21/75 (28%), Positives = 38/75 (50%)
 Frame = +1

Query: 178 VRIRQA*PLIRVMEDTIKTKEELQPAQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATR 357
           +  RQ+  L  V E+T +  + L  +QP +      +L  SD   ++L+  N ++ +A  
Sbjct: 441 IEARQSEKLNEVQEETQRLSQLLISSQPALTEVKHLKLCLSDSQEELLQL-NAKLEKANI 499

Query: 358 KLNPLTNNKAMLKLN 402
            ++ L  N A LKL+
Sbjct: 500 VIDEL--NSAKLKLS 512


>SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate
           synthase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 584

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 13/24 (54%), Positives = 15/24 (62%), Gaps = 3/24 (12%)
 Frame = +2

Query: 209 GSWKTPSKQKKNFN---LPNRRWP 271
           G  K PSK+ K F    LPNR+WP
Sbjct: 14  GMLKDPSKKYKPFKGIQLPNRQWP 37


>SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subunit
           8|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 230

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 11/48 (22%), Positives = 25/48 (52%)
 Frame = +1

Query: 229 KTKEELQPAQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPL 372
           + K++ Q ++ + +  ++ RL +  E+R  +   NK+ +    KL  L
Sbjct: 173 RLKQKKQRSEFRASKREMERLERDQELRNKIEERNKKELETIEKLREL 220


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
           Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 19/59 (32%), Positives = 27/59 (45%)
 Frame = +1

Query: 229 KTKEELQPAQPKMANTDLTRLLKSDEIRKVLRAPNKRVIRATRKLNPLTNNKAMLKLNP 405
           K K  L   Q K     LT+   S E + VL       +R T+K   LTN+  ++K +P
Sbjct: 730 KQKAHLSADQCKQLANVLTQA--SPEAKTVLEQHRLSEMRKTKKQTELTNSAHVIKPSP 786


>SPAC8E11.01c
           ||SPAC959.01|beta-fructofuranosidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 508

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 10/39 (25%), Positives = 20/39 (51%)
 Frame = -2

Query: 411 RVRIEFQHRLVISEWVQFACSTDHAFVGSTEDLPDLIRL 295
           + ++ F H L + +WV+ +       +G   + PD +RL
Sbjct: 171 KYKVLFYHSLNLRDWVKLSEFGSAGVLGYQYECPDFVRL 209


>SPAC22G7.08 |ppk8||serine/threonine protein kinase Ppk8
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 513

 Score = 25.4 bits (53), Expect = 7.9
 Identities = 13/39 (33%), Positives = 17/39 (43%), Gaps = 2/39 (5%)
 Frame = +2

Query: 215 WKTPSKQKKNFNL--PNRRWPTLTSHVFSSLMRSGRSSV 325
           WK P    K FNL    R  P   S +  SL  + R ++
Sbjct: 444 WKVPHTSDKRFNLYVTQRNDPNTKSQLIESLPMNSRDTI 482


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,340,499
Number of Sequences: 5004
Number of extensions: 43916
Number of successful extensions: 128
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 126
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -