BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30280 (440 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 33 0.015 SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|ch... 27 1.7 SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pom... 26 2.2 SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subuni... 26 3.0 SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating tra... 26 3.0 SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyce... 25 3.9 SPAC1F7.09c |||allantoicase |Schizosaccharomyces pombe|chr 1|||M... 25 6.8 SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizo... 25 6.8 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 24 9.0 SPCP1E11.10 |||ankyrin repeat protein, unknown biological role|S... 24 9.0 SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharo... 24 9.0 >SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schizosaccharomyces pombe|chr 1|||Manual Length = 322 Score = 33.5 bits (73), Expect = 0.015 Identities = 16/44 (36%), Positives = 25/44 (56%) Frame = +3 Query: 117 YSNGDVCDKTGRPRQTEVKLKCLENSSSPAQVSLYLLEPKTCHY 248 + NG +CD T RPR + +C NS +P +++ Y E +C Y Sbjct: 197 FRNGTMCDITKRPRHVILSYECSTNSDTP-EITQY-QEVSSCAY 238 >SPAC6G9.06c |pcp1||pericentrin Pcp1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1208 Score = 26.6 bits (56), Expect = 1.7 Identities = 12/39 (30%), Positives = 25/39 (64%), Gaps = 1/39 (2%) Frame = +2 Query: 290 LADENGL-IKTVREALEKKKERSEAVDDVSDKNVPKYEN 403 + DE+ L +KT+REAL+ K + + + ++N +Y++ Sbjct: 1029 VTDESQLMLKTLREALQSKTNNIDHLSTILERNRKEYKS 1067 >SPBC16E9.02c |||CUE domain protein Cue5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 569 Score = 26.2 bits (55), Expect = 2.2 Identities = 25/92 (27%), Positives = 36/92 (39%) Frame = +2 Query: 119 FERRRLRQNRPTPADRGQT*MSREFVESSSSLPVLVGT*NLSLYTGVESPLICDILPLAD 298 FER RL + R +R VESSSS + G + G + + D Sbjct: 244 FERLRLESSSSPMMRRSSLNSNRRSVESSSSAAFVEGQSFILDSNGAIE--VANSAFALD 301 Query: 299 ENGLIKTVREALEKKKERSEAVDDVSDKNVPK 394 ++ L E LE KK+ S+ + V K Sbjct: 302 DSDLESAYNEELEMKKDTSKPTASTKEVVVEK 333 >SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subunit Apc5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 737 Score = 25.8 bits (54), Expect = 3.0 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +2 Query: 68 RESSETYRSKNFNIAFLFERRRLRQNRPTP 157 RES +T NF +A++FE +R + + P+P Sbjct: 324 RESGDT-ACLNFALAWMFEFKRSQYSNPSP 352 >SPBC17G9.05 |rct1|cyp6|RRM-containing cyclophilin regulating transcription Rct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 432 Score = 25.8 bits (54), Expect = 3.0 Identities = 12/38 (31%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = +3 Query: 183 LENSSSPAQVSLYLLE-PKTCHYILAWNRL*YATYCPW 293 +E + + L++ E PKTC L +L Y +CP+ Sbjct: 5 IETTVGDLVIDLFVKEAPKTCENFLKLCKLKYYNFCPF 42 >SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyces pombe|chr 2|||Manual Length = 1112 Score = 25.4 bits (53), Expect = 3.9 Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 1/52 (1%) Frame = +2 Query: 254 GVESPLICDILPLA-DENGLIKTVREALEKKKERSEAVDDVSDKNVPKYEND 406 G+E+P+I +LP+ DE L LE + E DD + N +D Sbjct: 571 GIENPIISPLLPVKDDEKDLDLRELNPLEPFENMKEQADDGTVTNPLNVSSD 622 >SPAC1F7.09c |||allantoicase |Schizosaccharomyces pombe|chr 1|||Manual Length = 342 Score = 24.6 bits (51), Expect = 6.8 Identities = 12/34 (35%), Positives = 15/34 (44%) Frame = +3 Query: 24 RFDEKAHLDWIKDNKGKAPKPIDQRISISHFYSN 125 R EK H+DW+ G D I +HF N Sbjct: 234 RSREKGHVDWVIVKLGARGYIDDALIDTNHFKGN 267 >SPBC19C2.01 |cdc28|prp8|ATP-dependent RNA helicase Cdc28 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1055 Score = 24.6 bits (51), Expect = 6.8 Identities = 9/22 (40%), Positives = 13/22 (59%) Frame = +3 Query: 27 FDEKAHLDWIKDNKGKAPKPID 92 FDE +D++ D K A P+D Sbjct: 377 FDESQQIDFLLDTKLSAENPVD 398 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 24.2 bits (50), Expect = 9.0 Identities = 11/38 (28%), Positives = 23/38 (60%) Frame = +2 Query: 275 CDILPLADENGLIKTVREALEKKKERSEAVDDVSDKNV 388 C+I + D+N ++KT + + KK+ ++ V DK++ Sbjct: 1029 CEICEINDKNEIVKTRSQRIHSKKKAFLSL--VPDKSI 1064 >SPCP1E11.10 |||ankyrin repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 3|||Manual Length = 207 Score = 24.2 bits (50), Expect = 9.0 Identities = 18/80 (22%), Positives = 37/80 (46%), Gaps = 5/80 (6%) Frame = +3 Query: 54 IKDNKGKAPKPIDQRISISHFYSNGDVCDKTGRPRQTEVKLKCLENSSSPAQVSLYL--- 224 I+D G+ P + +++ I+H N D T + + + +E + +++ YL Sbjct: 65 IRDQDGETPLFVCEKLEIAHDLINQYNADTTVKNNDGLIAAQVIEANGEFPELAKYLYSF 124 Query: 225 --LEPKTCHYILAWNRL*YA 278 LEPK + + ++ YA Sbjct: 125 TDLEPKDVNTLPNDTKIEYA 144 >SPCC63.04 |mok14||alpha-1,3-glucan synthase Mok14|Schizosaccharomyces pombe|chr 3|||Manual Length = 1369 Score = 24.2 bits (50), Expect = 9.0 Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 3/36 (8%) Frame = -1 Query: 290 RAIC---RISKAIPRQYIMTGFRFQQVQGDLSWTRR 192 R +C +SK I R+YI G F + +S+ RR Sbjct: 430 REVCGLFNVSKTICREYIQFGNAFNLMHCGVSYVRR 465 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,701,589 Number of Sequences: 5004 Number of extensions: 31248 Number of successful extensions: 111 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 110 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 111 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 160149590 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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