BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30279 (508 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17G6.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 28 0.70 SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 27 1.2 SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosacc... 26 3.7 SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pom... 25 6.5 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 6.5 >SPAC17G6.13 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 433 Score = 28.3 bits (60), Expect = 0.70 Identities = 27/100 (27%), Positives = 39/100 (39%), Gaps = 1/100 (1%) Frame = +2 Query: 83 AVRDPVTGQLILHYSQIDPSHFEDKSGNYTIEKETQDLPANIDIQTVNTHKFSTFG-KAK 259 +VRDP G L +H +Q + K+G + Q P + + T S G + Sbjct: 322 SVRDPDDGTLPIHSTQTVEEPIDTKAGEKLTDSSKQPSPPVAEHFSNETFSASPKGAETD 381 Query: 260 VHKEATNPKPSQSTRRANTSGHQGYVSSNTPLSSKIPVSQ 379 V E +N S ++ V SN S K P SQ Sbjct: 382 VKSEGSNNHEQGSFNEPKSN-----VDSNDSASPKRPSSQ 416 >SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1184 Score = 27.5 bits (58), Expect = 1.2 Identities = 16/54 (29%), Positives = 24/54 (44%), Gaps = 3/54 (5%) Frame = +2 Query: 134 DPSHFEDKSGNYTIEKETQDLPANI--DIQTVNTHKFSTFGKAK-VHKEATNPK 286 + SHF + SGN E+ Q+ D+ T + FG++ H A N K Sbjct: 806 ESSHFSESSGNNEFEETEQEYTHGYGNDVLVQRTDVVNNFGESSHAHDNAVNEK 859 Score = 25.0 bits (52), Expect = 6.5 Identities = 12/36 (33%), Positives = 17/36 (47%) Frame = +2 Query: 269 EATNPKPSQSTRRANTSGHQGYVSSNTPLSSKIPVS 376 E T + T + GHQ +S T ++ IPVS Sbjct: 957 EGTEDNIVRQTTTVDEDGHQEQTTSTTKVAHPIPVS 992 >SPAC328.06 |ubp2||ubiquitin C-terminal hydrolase Ubp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1141 Score = 25.8 bits (54), Expect = 3.7 Identities = 20/67 (29%), Positives = 28/67 (41%) Frame = -3 Query: 443 STVCFRWSSSRRPAAWESSWRVGKRGS*RIKECWKTRSPGALMCSLVVYSVKVLGSSPLC 264 ++ C +WSS + E WR + IKE +R P + L S GSS Sbjct: 122 TSTCMKWSSKTTKSEIEVEWRDSYLDANCIKEIIDSRRP-SFASLLTKKSSSHQGSSHSS 180 Query: 263 GLSLYRT 243 SL+ T Sbjct: 181 QPSLFTT 187 >SPCP31B10.06 |mug190||C2 domain protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 1188 Score = 25.0 bits (52), Expect = 6.5 Identities = 16/68 (23%), Positives = 32/68 (47%) Frame = +2 Query: 149 EDKSGNYTIEKETQDLPANIDIQTVNTHKFSTFGKAKVHKEATNPKPSQSTRRANTSGHQ 328 E++ T ++ D D++ + H F AK + + + P+QS + ++ + Sbjct: 44 EEQQREQTENEKLFDPITQRDVEINDVH----FDYAKTYDDPSFTVPNQSIQGSSLPSEK 99 Query: 329 GYVSSNTP 352 Y+SSN P Sbjct: 100 PYLSSNQP 107 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 25.0 bits (52), Expect = 6.5 Identities = 18/62 (29%), Positives = 30/62 (48%), Gaps = 3/62 (4%) Frame = +2 Query: 194 LPANIDIQTVNTHKFSTFGKAKVHKEATNPKPSQSTRRANT---SGHQGYVSSNTPLSSK 364 LP+ + +T +TF AK E +N P+ + ++ +T S V SN+ LS Sbjct: 502 LPSTLQEETATGTTSTTFANAKRRPEDSNISPT-NPKKLHTLPRSPQYSTVDSNSVLSPA 560 Query: 365 IP 370 +P Sbjct: 561 MP 562 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,023,424 Number of Sequences: 5004 Number of extensions: 39831 Number of successful extensions: 106 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 105 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 106 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 202220600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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