Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30276
         (639 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual      56   3e-09
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo...    42   1e-04
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    42   1e-04
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar...    41   1e-04
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr...    30   0.32 
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po...    27   2.3  
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar...    26   5.3  
SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1 |Schizos...    26   5.3  
SPCC1020.10 |oca2||serine/threonine protein kinase Oca2 |Schizos...    25   7.0  
SPAC14C4.15c ||SPAPJ760.01c|dipeptidyl aminopeptidase |Schizosac...    25   9.2  
SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr 1||...    25   9.2  
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c...    25   9.2  
SPCC777.14 |prp4||serine/threonine protein kinase Prp4|Schizosac...    25   9.2  

>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 56.4 bits (130), Expect = 3e-09
 Identities = 23/71 (32%), Positives = 36/71 (50%)
 Frame = +2

Query: 5   IPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNEPWHRECFTCTNCNTSLAGQRFT 184
           I R+   +C  CY++K+A +C KC K I    V   +  +H +C+TC  CN  L  + + 
Sbjct: 358 IYRDDLYWCQTCYDNKYAVKCKKCRKPILGISVKGSDGEYHSQCWTCGACNALLGDEGYF 417

Query: 185 SRDEKPYCAEC 217
             +  P C  C
Sbjct: 418 MIENTPICRPC 428



 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 23/85 (27%), Positives = 35/85 (41%), Gaps = 1/85 (1%)
 Frame = +2

Query: 2   FIPREQEIYCAGCYEDKFATRCVKCNKIITQGGVTYKNEPWHRECFTCTNCNTSL-AGQR 178
           F  RE + YC   Y ++F+ RC  C   I    V   N+ +H     C  C+        
Sbjct: 297 FYYREGKFYCHLDYHEQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIP 356

Query: 179 FTSRDEKPYCAECFGELFAKRCTSC 253
              RD+  +C  C+   +A +C  C
Sbjct: 357 CIYRDDLYWCQTCYDNKYAVKCKKC 381



 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 20/72 (27%), Positives = 30/72 (41%), Gaps = 1/72 (1%)
 Frame = +2

Query: 41  YEDKFATRCVKCNKIITQGGV-TYKNEPWHRECFTCTNCNTSLAGQRFTSRDEKPYCAEC 217
           Y       C  C   +  G + +   +  H +CF C  C+ +L    F  R+ K YC   
Sbjct: 250 YRGNSEKSCHSCGGSLRAGRIISASGKKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLD 309

Query: 218 FGELFAKRCTSC 253
           + E F+ RC  C
Sbjct: 310 YHEQFSPRCKHC 321


>SPAC29A4.11 |rga3||GTPase activating protein
           Rga3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 969

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 21/73 (28%), Positives = 36/73 (49%), Gaps = 5/73 (6%)
 Frame = +2

Query: 50  KFATRCVKCNKIIT--QGGVTYKNEPWHRECFTCTNCNTSL--AGQRFT-SRDEKPYCAE 214
           K +T C +C +     +  +++    WH++CF CT C+  L  + Q    + D +P C+ 
Sbjct: 14  KGSTVCFRCGQAFQRRETPISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRPVCSS 73

Query: 215 CFGELFAKRCTSC 253
           C     A  CT+C
Sbjct: 74  C-----AHTCTAC 81



 Score = 35.9 bits (79), Expect = 0.005
 Identities = 14/54 (25%), Positives = 22/54 (40%)
 Frame = +2

Query: 56  ATRCVKCNKIITQGGVTYKNEPWHRECFTCTNCNTSLAGQRFTSRDEKPYCAEC 217
           A  C  C   I    +    + +HRECF C +C   +    F   +   +C +C
Sbjct: 75  AHTCTACRMRIKDYALMSGYDSYHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128



 Score = 34.3 bits (75), Expect = 0.015
 Identities = 10/32 (31%), Positives = 17/32 (53%)
 Frame = +1

Query: 310 WHNDCFICAQCKTSLVGKGFITDGQDIICPEC 405
           +H +CF C  C+  ++   F  D + I C +C
Sbjct: 97  YHRECFRCHDCRKQIIDSNFKRDNRTIFCNDC 128



 Score = 32.7 bits (71), Expect = 0.046
 Identities = 16/44 (36%), Positives = 21/44 (47%), Gaps = 4/44 (9%)
 Frame = +1

Query: 289 ISFEDRHWHNDCFICAQCKTSLVGKGFI----TDGQDIICPECA 408
           ISF    WH DCF C +C   L     +    +DG+  +C  CA
Sbjct: 33  ISFGGHMWHKDCFCCTKCDKGLEHSDQMLVQTSDGRP-VCSSCA 75


>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 22/76 (28%), Positives = 31/76 (40%), Gaps = 1/76 (1%)
 Frame = +2

Query: 29  CAGCYEDKFATRCVKCNKIITQGGVTYKNEPWHRECFTCTNCNTSLA-GQRFTSRDEKPY 205
           C   Y  +    C  C   +    +T  N+ +H E FTC+ C T       +   + K Y
Sbjct: 168 CETDYFRRLDLLCASCGMALRGYYITALNKKFHIEHFTCSLCYTVFGPNDSYYEYEGKVY 227

Query: 206 CAECFGELFAKRCTSC 253
           C   +  LFA RC  C
Sbjct: 228 CHYHYSTLFAARCCGC 243



 Score = 36.7 bits (81), Expect = 0.003
 Identities = 19/68 (27%), Positives = 29/68 (42%), Gaps = 5/68 (7%)
 Frame = +2

Query: 65  CVKCNKIITQGGVTYKNEPWHRECFTCTNCNTSLAGQRF-----TSRDEKPYCAECFGEL 229
           C  C ++I+   V      +H ECF C +CN+ +A + F     T   + P C   +   
Sbjct: 116 CASCGQVISGQYVRALGNIYHLECFRCHDCNSLVASKFFPIDDPTLNKQVPLCETDYFRR 175

Query: 230 FAKRCTSC 253
               C SC
Sbjct: 176 LDLLCASC 183



 Score = 29.9 bits (64), Expect = 0.32
 Identities = 16/45 (35%), Positives = 22/45 (48%), Gaps = 4/45 (8%)
 Frame = +2

Query: 14  EQEIYCAGCYEDKFATRCVKCN-KIITQGGVTYKN---EPWHREC 136
           E ++YC   Y   FA RC  C+  I+ Q    Y+N   + WH  C
Sbjct: 223 EGKVYCHYHYSTLFAARCCGCDGPILRQFVEVYRNGVSQNWHVPC 267


>SPBC28E12.03 |rga4||GTPase activating protein
           Rga4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 933

 Score = 41.1 bits (92), Expect = 1e-04
 Identities = 18/55 (32%), Positives = 29/55 (52%), Gaps = 4/55 (7%)
 Frame = +2

Query: 65  CVKC-NKIITQGGVTYKNEPWHRECFTCTNCNTSL--AGQRFTSRDEKP-YCAEC 217
           C+KC   + +   V +  + WH +CF C NCN  L  + + F+  D+K  +C  C
Sbjct: 24  CIKCWESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLC 78



 Score = 31.5 bits (68), Expect = 0.11
 Identities = 16/48 (33%), Positives = 21/48 (43%), Gaps = 3/48 (6%)
 Frame = +1

Query: 271 IGGTRFISFEDRHWHNDCFICAQCKTSL--VGKGFITDGQ-DIICPEC 405
           +  T  + F  + WH+DCF C  C   L    + F  D Q  I C  C
Sbjct: 31  VPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLC 78


>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 131

 Score = 29.9 bits (64), Expect = 0.32
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +1

Query: 307 HWHNDCFICAQCKTSLVGKGFI 372
           H  + C++CA+CKT L  KG +
Sbjct: 8   HLKSRCYVCAKCKTHLAFKGHL 29


>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 897

 Score = 27.1 bits (57), Expect = 2.3
 Identities = 10/27 (37%), Positives = 14/27 (51%)
 Frame = +2

Query: 137 FTCTNCNTSLAGQRFTSRDEKPYCAEC 217
           FTC  C + L GQ++ S       A+C
Sbjct: 625 FTCQECMSMLVGQKYGSSSTSTIIAKC 651


>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
           1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 232

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 9/22 (40%), Positives = 16/22 (72%), Gaps = 1/22 (4%)
 Frame = -2

Query: 521 LHITKHLSYINCIACE-GWSDA 459
           ++  KHL+++NCI C   W++A
Sbjct: 205 VYCCKHLAHVNCINCNTPWANA 226


>SPCC1902.01 |gaf1|SPCC417.01c|transcription factor Gaf1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 855

 Score = 25.8 bits (54), Expect = 5.3
 Identities = 11/26 (42%), Positives = 12/26 (46%)
 Frame = +2

Query: 140 TCTNCNTSLAGQRFTSRDEKPYCAEC 217
           TCTNC T        S D +P C  C
Sbjct: 634 TCTNCQTRTTPLWRRSPDGQPLCNAC 659


>SPCC1020.10 |oca2||serine/threonine protein kinase Oca2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 650

 Score = 25.4 bits (53), Expect = 7.0
 Identities = 9/17 (52%), Positives = 12/17 (70%)
 Frame = +3

Query: 342 QDLARRQGLHHRRTGHH 392
           Q + R+ GLHH + GHH
Sbjct: 213 QFIFRKLGLHHGKHGHH 229


>SPAC14C4.15c ||SPAPJ760.01c|dipeptidyl aminopeptidase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 853

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/33 (27%), Positives = 18/33 (54%)
 Frame = -2

Query: 464 DARAEALKRDRCIYMSFCLAHSGQMMSCPSVMK 366
           +A+    K+ R IY++ CL     ++SC  + +
Sbjct: 55  EAKKRRRKKHRYIYLAVCLFFLASVLSCAIIFR 87


>SPAC29B12.12 |||helper of TIM |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 113

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/23 (39%), Positives = 12/23 (52%)
 Frame = +1

Query: 286 FISFEDRHWHNDCFICAQCKTSL 354
           F+ +    +H  C IC  CK SL
Sbjct: 58  FLPWRKAKFHIPCVICGACKNSL 80


>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1111

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 9/14 (64%), Positives = 10/14 (71%)
 Frame = -3

Query: 262 WACARSAALGEQFP 221
           WACARS  LG + P
Sbjct: 595 WACARSYGLGTRLP 608


>SPCC777.14 |prp4||serine/threonine protein kinase
           Prp4|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 477

 Score = 25.0 bits (52), Expect = 9.2
 Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 3/38 (7%)
 Frame = -2

Query: 377 SVMKPLPTS---EVLHCAQMKQSLCQCLSSNEMKRVPP 273
           S +K +PTS   E +   ++   L +CL  N  KRVPP
Sbjct: 428 SRLKEVPTSTDEEFIIRQELMDLLEKCLELNPEKRVPP 465


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,205,339
Number of Sequences: 5004
Number of extensions: 41940
Number of successful extensions: 153
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 285732116
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)