Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30272
(573 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) simi... 127 6e-30
At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB) 125 2e-29
At3g61035.1 68416.m06829 cytochrome P450 family protein similar ... 31 0.41
At4g33010.1 68417.m04695 glycine dehydrogenase [decarboxylating]... 29 2.2
At3g19050.1 68416.m02420 kinesin motor protein-related contains ... 28 5.1
At1g54570.1 68414.m06223 esterase/lipase/thioesterase family pro... 27 6.7
At1g30730.1 68414.m03756 FAD-binding domain-containing protein s... 27 6.7
At5g38550.1 68418.m04661 jacalin lectin family protein similar t... 27 8.9
At2g21630.1 68415.m02573 transport protein, putative similar to ... 27 8.9
At1g30720.1 68414.m03755 FAD-binding domain-containing protein s... 27 8.9
>At3g04840.1 68416.m00525 40S ribosomal protein S3A (RPS3aA) similar
to 40S ribosomal protein S3A (S phase specific protein
GBIS289) GB:P49396 [Brassica rapa]
Length = 262
Score = 127 bits (306), Expect = 6e-30
Identities = 57/90 (63%), Positives = 70/90 (77%)
Frame = +1
Query: 4 ERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRV 183
+ ++RK RL AE VQGRNVLC F GMD TTDKLR +VKKWQTLIEA++DVKTTD Y LR+
Sbjct: 78 DNAYRKIRLRAEDVQGRNVLCQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDSYTLRL 137
Query: 184 FCIGFTNKDSLSQRKTCYAQHTQVEQSERK 273
FCI FT + + ++TCYAQ +Q+ Q RK
Sbjct: 138 FCIAFTKRRANQVKRTCYAQSSQIRQIRRK 167
Score = 86.2 bits (204), Expect = 1e-17
Identities = 36/71 (50%), Positives = 58/71 (81%)
Frame = +3
Query: 255 RAIRKKMCEIITRDVTNSELREVVNKLIPDSIAKDIEKACHGIYPLRDVCIRKVKVLKRP 434
R IR+KM +I+ R+ ++ +L+++V K IP++I ++IEKA GIYPL++V IRKVK+LK P
Sbjct: 162 RQIRRKMRDIMVREASSCDLKDLVAKFIPEAIGREIEKATQGIYPLQNVFIRKVKILKAP 221
Query: 435 RFEISKLMELH 467
+F++ KLM++H
Sbjct: 222 KFDLGKLMDVH 232
>At4g34670.1 68417.m04922 40S ribosomal protein S3A (RPS3aB)
Length = 262
Score = 125 bits (302), Expect = 2e-29
Identities = 57/90 (63%), Positives = 70/90 (77%)
Frame = +1
Query: 4 ERSFRKFRLIAEYVQGRNVLCNFHGMDLTTDKLRWMVKKWQTLIEANIDVKTTDGYVLRV 183
+ ++RK RL AE VQGRNVL F GMD TTDKLR +VKKWQTLIEA++DVKTTDGY LR+
Sbjct: 78 DNAYRKIRLRAEDVQGRNVLTQFWGMDFTTDKLRSLVKKWQTLIEAHVDVKTTDGYTLRM 137
Query: 184 FCIGFTNKDSLSQRKTCYAQHTQVEQSERK 273
FCI FT + + ++TCYAQ +Q+ Q RK
Sbjct: 138 FCIAFTKRRANQVKRTCYAQSSQIRQIRRK 167
Score = 89.4 bits (212), Expect = 1e-18
Identities = 38/71 (53%), Positives = 58/71 (81%)
Frame = +3
Query: 255 RAIRKKMCEIITRDVTNSELREVVNKLIPDSIAKDIEKACHGIYPLRDVCIRKVKVLKRP 434
R IR+KM EI+ ++ ++ +L+E+V K IP++I ++IEKA GIYPL++V IRKVK+LK P
Sbjct: 162 RQIRRKMSEIMVKEASSCDLKELVAKFIPEAIGREIEKATQGIYPLQNVFIRKVKILKAP 221
Query: 435 RFEISKLMELH 467
+F++ KLME+H
Sbjct: 222 KFDLGKLMEVH 232
>At3g61035.1 68416.m06829 cytochrome P450 family protein similar to
Cytochrome P450 76C2 (SP:O64637) [Arabidopsis thaliana]
Length = 340
Score = 31.5 bits (68), Expect = 0.41
Identities = 20/58 (34%), Positives = 29/58 (50%)
Frame = -2
Query: 413 LSDANIAQRVDAMAGLLDVLGNGVRNQLVHHLPEFRVSDVACNDFTHFLSDCST*VCW 240
L DA IA+R + +D L + + ++ + E S+V ND HFL ST V W
Sbjct: 250 LIDARIAERSSQIGPRVDALRGDLLDSVIDLIQE-EGSEVDMNDIKHFLCTNSTIVEW 306
>At4g33010.1 68417.m04695 glycine dehydrogenase [decarboxylating],
putative / glycine decarboxylase, putative / glycine
cleavage system P-protein, putative strong similarity to
SP|P49361 Glycine dehydrogenase [decarboxylating] A,
mitochondrial precursor (EC 1.4.4.2) {Flaveria
pringlei}; contains Pfam profile PF02347: Glycine
cleavage system P-protein
Length = 1037
Score = 29.1 bits (62), Expect = 2.2
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = -2
Query: 401 NIAQRVDAMAGLLDVLGNGVRNQLVHHLPEFRVSDVACNDFTHFLSDCST 252
+IAQRV +AG+ + N + V LP F + C+D H ++D ++
Sbjct: 426 SIAQRVHGLAGIFSLGLNKLGVAEVQELPFFDTVKIKCSD-AHAIADAAS 474
>At3g19050.1 68416.m02420 kinesin motor protein-related contains Pfam
profile: PF00225 Kinesin motor domain; contains
non-consensus splice site (GC) at intron 12
Length = 2722
Score = 27.9 bits (59), Expect = 5.1
Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = +3
Query: 210 LLEPTQDVLRPAHSGRAIRKKMCEIITRDVTNSEL-REVVNKLIPDSIAKDIEKACHGIY 386
L E ++ + + G AI KM ++I +V + + + V S+ D+++
Sbjct: 1985 LTEDSKKLEKLTRDGMAISDKMLQLICENVDKASVFADTVQ-----SLQIDVQELLSENL 2039
Query: 387 PLRDVCIRKVKVLKRPRFEISKLME 461
L D +RK VLK F++S L E
Sbjct: 2040 NLHDELLRKDDVLKGLSFDLSLLQE 2064
>At1g54570.1 68414.m06223 esterase/lipase/thioesterase family
protein contains Interpro entry IPR000379
Length = 704
Score = 27.5 bits (58), Expect = 6.7
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = -2
Query: 512 VRLVSGFPLASTAFAVKFHQLRDLETGPLQHFHLSDANIAQR 387
V+ ++G P V +H L LE GP+ + + NI R
Sbjct: 435 VKGLAGVPDKGPVLLVGYHMLMGLELGPMSEAFIKEKNILFR 476
>At1g30730.1 68414.m03756 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 526
Score = 27.5 bits (58), Expect = 6.7
Identities = 17/63 (26%), Positives = 31/63 (49%)
Frame = +3
Query: 150 CEDNRWIRSTCLLHWFHQ*GLLEPTQDVLRPAHSGRAIRKKMCEIITRDVTNSELREVVN 329
C + RWI S +L W + PT +L P + + K+ + + R V+ + L ++
Sbjct: 334 CTEMRWIDS--VLFWAGY-PVGTPTSVLLNPTVTKKLFMKRKSDYVKRPVSRTGLGLILK 390
Query: 330 KLI 338
KL+
Sbjct: 391 KLV 393
>At5g38550.1 68418.m04661 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 594
Score = 27.1 bits (57), Expect = 8.9
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +2
Query: 344 LHCQGHREGLPW 379
L CQG R+GLPW
Sbjct: 299 LECQGDRKGLPW 310
>At2g21630.1 68415.m02573 transport protein, putative similar to
Swiss-Prot:Q15436 protein transport protein Sec23A [Homo
sapiens]
Length = 761
Score = 27.1 bits (57), Expect = 8.9
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = -2
Query: 125 CHFLTIHLSLSVVRSMPWKLQSTLRPCTYSAINLNLRKDL 6
CHF T+H L + + PW + + RP + + L + L
Sbjct: 238 CHF-TLHSVLEELGNSPWPVAADHRPARCTGVALRIAASL 276
>At1g30720.1 68414.m03755 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 27.1 bits (57), Expect = 8.9
Identities = 16/63 (25%), Positives = 32/63 (50%)
Frame = +3
Query: 150 CEDNRWIRSTCLLHWFHQ*GLLEPTQDVLRPAHSGRAIRKKMCEIITRDVTNSELREVVN 329
C + RWI S +L W + PT +L P + + K+ + + R ++ ++L ++
Sbjct: 335 CTEMRWIDS--VLFWDDY-PVGTPTSVLLNPLVAKKLFMKRKSDYVKRLISRTDLGLILK 391
Query: 330 KLI 338
KL+
Sbjct: 392 KLV 394
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,521,858
Number of Sequences: 28952
Number of extensions: 256664
Number of successful extensions: 746
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 746
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1112061928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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