Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30266
(708 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 158 6e-40
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 156 3e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 100 2e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 73 4e-14
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.5
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 26 4.6
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.1
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 26 6.1
SPBC119.07 |ppk19||serine/threonine protein kinase Ppk19|Schizos... 25 8.0
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 25 8.0
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.0
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 158 bits (384), Expect = 6e-40
Identities = 68/100 (68%), Positives = 82/100 (82%)
Frame = +2
Query: 218 FQHFLQRDRSWQDVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 397
F F + VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 398 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 517
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFGGGT G
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSG 148
Score = 109 bits (262), Expect = 4e-25
Identities = 47/66 (71%), Positives = 60/66 (90%)
Frame = +1
Query: 511 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 690
SGF +LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+LTTH TL+ +DC FMVDN
Sbjct: 147 SGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDN 206
Query: 691 EAIYDI 708
E+ YDI
Sbjct: 207 ESCYDI 212
Score = 98.7 bits (235), Expect = 7e-22
Identities = 43/63 (68%), Positives = 47/63 (74%)
Frame = +3
Query: 75 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 254
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 255 TYP 263
P
Sbjct: 61 YVP 63
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 156 bits (379), Expect = 3e-39
Identities = 65/100 (65%), Positives = 83/100 (83%)
Frame = +2
Query: 218 FQHFLQRDRSWQDVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 397
F F + VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 398 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 517
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFGGGT G
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSG 152
Score = 112 bits (269), Expect = 5e-26
Identities = 48/66 (72%), Positives = 60/66 (90%)
Frame = +1
Query: 511 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 690
SG +LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+LTTH TL++SDC FMVDN
Sbjct: 151 SGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDN 210
Query: 691 EAIYDI 708
EA YDI
Sbjct: 211 EACYDI 216
Score = 92.7 bits (220), Expect = 5e-20
Identities = 47/68 (69%), Positives = 49/68 (72%), Gaps = 5/68 (7%)
Frame = +3
Query: 75 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 239
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 240 TGAGKTYP 263
TG GK P
Sbjct: 60 TGQGKFVP 67
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 100 bits (240), Expect = 2e-22
Identities = 45/87 (51%), Positives = 59/87 (67%)
Frame = +2
Query: 257 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDR 436
VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 60 VPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDV 119
Query: 437 IRKLADQCTGLQGFLIFHSFGGGTALG 517
+R+ A+ C LQGF + HS GGGT G
Sbjct: 120 VRREAEACDALQGFQLTHSLGGGTGSG 146
Score = 60.9 bits (141), Expect = 2e-10
Identities = 27/66 (40%), Positives = 42/66 (63%)
Frame = +1
Query: 511 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDN 690
SG +LL+ ++ +Y + F++ PAP+ S VVEPYN+ L+ H +E+SD F +DN
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCIDN 204
Query: 691 EAIYDI 708
EA+ I
Sbjct: 205 EALSSI 210
Score = 40.7 bits (91), Expect = 2e-04
Identities = 21/63 (33%), Positives = 28/63 (44%)
Frame = +3
Query: 75 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 254
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 255 TYP 263
P
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 72.9 bits (171), Expect = 4e-14
Identities = 38/99 (38%), Positives = 59/99 (59%), Gaps = 2/99 (2%)
Frame = +2
Query: 227 FLQRDRSWQDVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 400
F Q D + + +PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 401 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTALG 517
+ I + ++D I + AD L+GF + HS GGT G
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSG 148
Score = 49.2 bits (112), Expect = 6e-07
Identities = 25/62 (40%), Positives = 35/62 (56%)
Frame = +3
Query: 78 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKT 257
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ +
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 258 YP 263
P
Sbjct: 61 IP 62
Score = 47.6 bits (108), Expect = 2e-06
Identities = 26/67 (38%), Positives = 39/67 (58%), Gaps = 1/67 (1%)
Frame = +1
Query: 511 SGFTSLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSILTTHTTLEHSDCAFMVD 687
SG S L+ERL+ Y KK ++++P Q VS VV+PYNS+L ++D ++D
Sbjct: 147 SGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNADSVVVLD 206
Query: 688 NEAIYDI 708
N A+ I
Sbjct: 207 NAALAHI 213
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.9 bits (59), Expect = 1.5
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -3
Query: 310 ADLINNSRFKIDEDS--TGYVLPAPVSLKKVLKESSP 206
AD+IN SR+ ID S T + P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 26.2 bits (55), Expect = 4.6
Identities = 14/58 (24%), Positives = 25/58 (43%), Gaps = 2/58 (3%)
Frame = -3
Query: 343 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIW 176
F + + S +++N+ + KID GY S K + E + P + G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +2
Query: 173 WPDAHRQDHRGWRRFFQHFLQR 238
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 6.1
Identities = 12/37 (32%), Positives = 23/37 (62%)
Frame = -3
Query: 277 DEDSTGYVLPAPVSLKKVLKESSPPPMVLSVGIWPSG 167
++D T Y+ P P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKYLGP-PIYTSQRLYDTTPPGVVMGLGWTPMG 869
>SPBC119.07 |ppk19||serine/threonine protein kinase
Ppk19|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1706
Score = 25.4 bits (53), Expect = 8.0
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +2
Query: 176 PDAHRQDHRGWRRFFQHFLQRDRSWQDVPRAVFVDLEPTV 295
P+ H + G F + F +DR DV VFV+ P +
Sbjct: 22 PEYHNERSLGDSHFLRTFRMQDRKGYDVLIKVFVNKLPEI 61
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 25.4 bits (53), Expect = 8.0
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = -3
Query: 562 SCRSQRRDAPSIRK*TQSGTSTEGVEDQESLQTGTLVSELADSVQN 425
S + SIR ++S +S+ G + Q S+Q+GT ++D ++
Sbjct: 98 SSQKSSNSRKSIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.0
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +1
Query: 64 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 186
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,997,981
Number of Sequences: 5004
Number of extensions: 64382
Number of successful extensions: 213
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 195
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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