Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30263
(697 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 137 1e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 89 7e-19
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 87 2e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 68 1e-12
SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa... 29 0.84
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.9
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 2.6
SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 3.4
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 3.4
SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase |Schizosaccha... 26 4.5
SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 26 5.9
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 7.8
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.8
SPBC11B10.01 |alg2|SPBC32H8.14|mannosyltransferase complex subun... 25 7.8
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 137 bits (332), Expect = 1e-33
Identities = 60/77 (77%), Positives = 70/77 (90%), Gaps = 1/77 (1%)
Frame = +3
Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 425
PR +LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 426 RKESESCDCLQGFQLTH 476
R+E+E+CD LQGFQLTH
Sbjct: 121 RREAEACDALQGFQLTH 137
Score = 111 bits (268), Expect = 7e-26
Identities = 46/63 (73%), Positives = 54/63 (85%)
Frame = +1
Query: 67 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 246
MREIVH+QAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 247 PRA 255
PRA
Sbjct: 61 PRA 63
Score = 91.1 bits (216), Expect = 1e-19
Identities = 41/50 (82%), Positives = 47/50 (94%)
Frame = +2
Query: 509 TLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDE 658
TLL+SKIREEYPDR+M T+SV P+PK SDTVVEPYNATLS+HQLVEN+DE
Sbjct: 149 TLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEPYNATLSMHQLVENSDE 198
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 88.6 bits (210), Expect = 7e-19
Identities = 38/77 (49%), Positives = 55/77 (71%), Gaps = 1/77 (1%)
Frame = +3
Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 425
PR I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++DSVL+ +
Sbjct: 67 PRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERI 126
Query: 426 RKESESCDCLQGFQLTH 476
R+ +++C LQGF + H
Sbjct: 127 RRMADNCSGLQGFLVFH 143
Score = 51.6 bits (118), Expect = 1e-07
Identities = 24/62 (38%), Positives = 39/62 (62%)
Frame = +2
Query: 512 LLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDENLLASTTKALY 691
LL+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++N+D + +A Y
Sbjct: 156 LLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVD-NEACY 214
Query: 692 DI 697
DI
Sbjct: 215 DI 216
Score = 48.8 bits (111), Expect = 7e-07
Identities = 24/69 (34%), Positives = 36/69 (52%), Gaps = 6/69 (8%)
Frame = +1
Query: 67 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 228
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 229 SGGKYVPRA 255
GK+VPR+
Sbjct: 61 GQGKFVPRS 69
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 87.4 bits (207), Expect = 2e-18
Identities = 38/77 (49%), Positives = 53/77 (68%), Gaps = 1/77 (1%)
Frame = +3
Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 425
PR I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D +
Sbjct: 63 PRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKI 122
Query: 426 RKESESCDCLQGFQLTH 476
R+ +++C LQGF + H
Sbjct: 123 RRIADNCSGLQGFLVFH 139
Score = 54.8 bits (126), Expect = 1e-08
Identities = 26/65 (40%), Positives = 37/65 (56%), Gaps = 2/65 (3%)
Frame = +1
Query: 67 MREIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLE--RINVYYNEASGGK 240
MREI+ + GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 241 YVPRA 255
YVPR+
Sbjct: 61 YVPRS 65
Score = 47.2 bits (107), Expect = 2e-06
Identities = 22/65 (33%), Positives = 38/65 (58%)
Frame = +2
Query: 503 YWTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDENLLASTTK 682
+ LL+ ++ EY + +SV P+P+VS +VVEPYN+ L+ H ++ D + +
Sbjct: 149 FGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMVD-NE 207
Query: 683 ALYDI 697
+ YDI
Sbjct: 208 SCYDI 212
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 68.1 bits (159), Expect = 1e-12
Identities = 29/79 (36%), Positives = 52/79 (65%), Gaps = 3/79 (3%)
Frame = +3
Query: 249 PR-ILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLD 419
PR IL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D
Sbjct: 62 PRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMD 120
Query: 420 VVRKESESCDCLQGFQLTH 476
++ +E++ D L+GF L H
Sbjct: 121 MIDREADGSDSLEGFSLLH 139
Score = 67.3 bits (157), Expect = 2e-12
Identities = 28/62 (45%), Positives = 43/62 (69%)
Frame = +1
Query: 70 REIVHLQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 249
REI+ LQAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 250 RA 255
RA
Sbjct: 63 RA 64
Score = 50.0 bits (114), Expect = 3e-07
Identities = 22/50 (44%), Positives = 34/50 (68%), Gaps = 1/50 (2%)
Frame = +2
Query: 509 TLLISKIREEYPDRIMNTYSVVP-SPKVSDTVVEPYNATLSVHQLVENTD 655
+ L+ ++ + YP +I+ TYSV P S VSD VV+PYN+ L++ +L N D
Sbjct: 151 SFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLLALKRLTLNAD 200
>SPAC1B9.02c |sck1||serine/threonine protein kinase
Sck1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 696
Score = 28.7 bits (61), Expect = 0.84
Identities = 15/59 (25%), Positives = 30/59 (50%)
Frame = -2
Query: 585 LGEGTTEYVFMILSGYSSRIFEMRRVQYRTRCRHRGNV*VGSPVGNRKILILCGLHRGP 409
+G+GT V+++ ++RI+ M+++ + R + V +G R IL+ L P
Sbjct: 308 IGKGTFGQVYLVRKNDTNRIYAMKKISKKLIVRKKE---VTHTLGERNILVRTSLDESP 363
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.9
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 369 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 458
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 2.6
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +1
Query: 106 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 207
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 682
Score = 26.6 bits (56), Expect = 3.4
Identities = 17/43 (39%), Positives = 26/43 (60%), Gaps = 2/43 (4%)
Frame = +1
Query: 466 NLHIPS--VAAPGPVLDPPHLKDP*RVPRQNHEHILSSPLAQS 588
N+H PS + L+P +++P +P+QNHEH SPL +S
Sbjct: 623 NIHHPSANLLDASAALNP--VQNPLLMPQQNHEH---SPLVRS 660
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 3.4
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 300 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 401
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC12G12.06c |||RNA 3'-terminal phosphate cyclase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 363
Score = 26.2 bits (55), Expect = 4.5
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +2
Query: 50 KITYTK*GKSFIYRPANVV 106
+I+YT G SFIYRP N++
Sbjct: 64 EISYT--GTSFIYRPGNII 80
>SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase
E3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1647
Score = 25.8 bits (54), Expect = 5.9
Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = +1
Query: 151 GIDPTGAYHGD--SDLQLERINVYYNEASGGKYVPRASSSTWSPAPWTLSAPD 303
G+ PT ++ + L ++++ N+ K+V +AS SP P S+P+
Sbjct: 1314 GLGPTLEFYTSVSKEFTLNSLDIWRNDQPNSKFVYQASGLFPSPIPLLGSSPE 1366
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 321 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 452
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.8
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 358 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 263
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
>SPBC11B10.01 |alg2|SPBC32H8.14|mannosyltransferase complex subunit
Alg2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 511
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/38 (28%), Positives = 19/38 (50%)
Frame = -2
Query: 624 RVALYGSTTVSDTLGEGTTEYVFMILSGYSSRIFEMRR 511
R+A+ + + D EY+ ++ GY R+ E RR
Sbjct: 242 RLAIDAFSALRDLSANRFPEYLLLVAGGYDIRVSENRR 279
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,790,589
Number of Sequences: 5004
Number of extensions: 56273
Number of successful extensions: 175
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 171
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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