Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30262
(751 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 186 3e-48
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 182 5e-47
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 101 1e-22
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 60 4e-10
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.025
SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces ... 27 2.9
SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 27 3.8
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 26 6.6
SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3 |Schizosacc... 26 6.6
SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 8.7
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 186 bits (453), Expect = 3e-48
Identities = 92/139 (66%), Positives = 100/139 (71%), Gaps = 2/139 (1%)
Frame = +1
Query: 256 NSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFD 435
NS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI Q+VSSITASLRF
Sbjct: 190 NSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIAQVVSSITASLRFA 249
Query: 436 GALNVDLTEFQTNLVPYPRIHFPLARTRQSSLPRRPTMNSFPSPRSQTHAFEPANQMVKC 615
G+LNVDL EFQTNLVPYPRIHFPL + S FEP NQMVKC
Sbjct: 250 GSLNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKC 309
Query: 616 DPRHGKYMG--LLYAVTVV 666
DPR G+YM LLY V+
Sbjct: 310 DPRTGRYMATCLLYRGDVI 328
Score = 122 bits (293), Expect = 7e-29
Identities = 52/83 (62%), Positives = 68/83 (81%)
Frame = +2
Query: 2 NNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVD 181
NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH LL+ERL+++
Sbjct: 105 NNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNME 164
Query: 182 YGKKSKLEFAIYPAPQVSTAVVE 250
YGKKS L+F++YPAPQVST+VVE
Sbjct: 165 YGKKSNLQFSVYPAPQVSTSVVE 187
Score = 93.1 bits (221), Expect = 4e-20
Identities = 47/93 (50%), Positives = 61/93 (65%), Gaps = 4/93 (4%)
Frame = +3
Query: 483 LPPYP---LPTGTYAPVISAEKAYHEQLSVAEITNACFRARQPDGEMRPPSWQVHGLAVC 653
L PYP P TY+P++SA KA+HE SV EITN CF + P + + +A C
Sbjct: 263 LVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRY--MATC 320
Query: 654 C-NRGDVVPKDVNAAIATIKTKRTIQFVDWCPT 749
RGDV+P+DV AA+ +IK++RTIQFVDWCPT
Sbjct: 321 LLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPT 353
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 182 bits (443), Expect = 5e-47
Identities = 89/139 (64%), Positives = 100/139 (71%), Gaps = 2/139 (1%)
Frame = +1
Query: 256 NSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFD 435
NS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI Q+VSSITASLRF+
Sbjct: 186 NSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIAQVVSSITASLRFE 245
Query: 436 GALNVDLTEFQTNLVPYPRIHFPLARTRQSSLPRRPTMNSFPSPRSQTHAFEPANQMVKC 615
G+LNVDL EFQTNLVPYPRIHFPL + S FEP NQMVKC
Sbjct: 246 GSLNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKC 305
Query: 616 DPRHGKYMG--LLYAVTVV 666
DPR G+YM LLY V+
Sbjct: 306 DPRAGRYMATCLLYRGDVI 324
Score = 119 bits (287), Expect = 4e-28
Identities = 52/83 (62%), Positives = 67/83 (80%)
Frame = +2
Query: 2 NNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVD 181
NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH LL+ERL+++
Sbjct: 101 NNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAME 160
Query: 182 YGKKSKLEFAIYPAPQVSTAVVE 250
Y KKSKL+F++YPAPQVST+VVE
Sbjct: 161 YTKKSKLQFSVYPAPQVSTSVVE 183
Score = 96.7 bits (230), Expect = 3e-21
Identities = 50/93 (53%), Positives = 60/93 (64%), Gaps = 4/93 (4%)
Frame = +3
Query: 483 LPPYP---LPTGTYAPVISAEKAYHEQLSVAEITNACFRARQPDGEMRPPSWQVHGLAVC 653
L PYP P TYAP++SA KA+HE SV EITN CF + P + + +A C
Sbjct: 259 LVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRY--MATC 316
Query: 654 C-NRGDVVPKDVNAAIATIKTKRTIQFVDWCPT 749
RGDV+P+DV AA+ TIK KRTIQFVDWCPT
Sbjct: 317 LLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPT 349
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 101 bits (241), Expect = 1e-22
Identities = 46/128 (35%), Positives = 71/128 (55%)
Frame = +1
Query: 256 NSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFD 435
N+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+ +++ +T S RF
Sbjct: 184 NATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVSAVMAGVTTSFRFP 243
Query: 436 GALNVDLTEFQTNLVPYPRIHFPLARTRQSSLPRRPTMNSFPSPRSQTHAFEPANQMVKC 615
G LN DL + N+VP+PR+HF + + + + P F+ N MV
Sbjct: 244 GELNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAA 303
Query: 616 DPRHGKYM 639
DPRHG+Y+
Sbjct: 304 DPRHGRYL 311
Score = 65.7 bits (153), Expect = 7e-12
Identities = 31/83 (37%), Positives = 46/83 (55%)
Frame = +2
Query: 2 NNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVD 181
N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H LL+ ++ +
Sbjct: 99 NIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREE 158
Query: 182 YGKKSKLEFAIYPAPQVSTAVVE 250
Y + F++ PAP+ S VVE
Sbjct: 159 YPDRMMATFSVAPAPKSSDTVVE 181
Score = 35.9 bits (79), Expect = 0.006
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +3
Query: 513 YAPVISAEKAYHEQLSVAEITNACFRARQPDGEMRPPSWQVHGLAVCCNRGDVVPKDVNA 692
+AP+ + + + +SV E+T F A P + +A RG V K+V+
Sbjct: 270 FAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALF-RGKVSMKEVDE 328
Query: 693 AIATIKTKRTIQFVDWCP 746
I +++TK + FV+W P
Sbjct: 329 QIRSVQTKNSAYFVEWIP 346
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 59.7 bits (138), Expect = 4e-10
Identities = 28/84 (33%), Positives = 46/84 (54%)
Frame = +1
Query: 256 NSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFD 435
NS+L ++D ++DN A+ I L + PT+ N+L+ ++S+ T +LR+
Sbjct: 187 NSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVMSASTTTLRYP 246
Query: 436 GALNVDLTEFQTNLVPYPRIHFPL 507
G +N DL +L+P PR HF L
Sbjct: 247 GYMNNDLVSIIASLIPSPRCHFLL 270
Score = 44.0 bits (99), Expect = 2e-05
Identities = 27/84 (32%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
Frame = +2
Query: 2 NNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVD 181
NN+A G Y+ + I + ++D I + AD L+GF + H L+ERL+
Sbjct: 102 NNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDR 160
Query: 182 YGKKSKLEFAIYPAPQ-VSTAVVE 250
Y KK ++++P Q VS VV+
Sbjct: 161 YPKKIIQTYSVFPNSQSVSDVVVQ 184
Score = 26.2 bits (55), Expect = 5.0
Identities = 9/29 (31%), Positives = 17/29 (58%)
Frame = +3
Query: 660 RGDVVPKDVNAAIATIKTKRTIQFVDWCP 746
+G+ P DV+ ++ I+ +R F+ W P
Sbjct: 325 QGEADPADVHKSLLRIRERRYASFIPWGP 353
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.025
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -2
Query: 483 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 322
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPAC589.09 |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 388
Score = 27.1 bits (57), Expect = 2.9
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = -2
Query: 522 LARTCQWEVDTGVRHQVSLELGEVHIQSAVESQ 424
L T QW VDTGV V E GE++ + + Q
Sbjct: 101 LINTLQWRVDTGVERIV--ERGELYAKETNDDQ 131
>SPAC343.18 |rfp2||ubiquitin-protein ligase E3
Rfp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 205
Score = 26.6 bits (56), Expect = 3.8
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 3/58 (5%)
Frame = +1
Query: 403 VSSITASLRFDGALNVDLTEFQTNLVP--YPRIHFPLARTR-QSSLPRRPTMNSFPSP 567
V +T + DGA ++T +P PR +R ++L PT+NS PSP
Sbjct: 19 VIDLTEDIEDDGADVSEVTLLDLTRIPEFQPRRRIRTSRNHLDANLSNVPTINSIPSP 76
>SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 542
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/37 (35%), Positives = 15/37 (40%)
Frame = -3
Query: 683 ILGYDVTTVTAYSKPMYLP*RGSHFTIWLAGSKACVC 573
I G T VT YS P+ S WL C+C
Sbjct: 73 ISGLFATVVTTYSYPLISGGAPSAVWCWLIAGAGCMC 109
>SPCC1223.01 ||SPCC285.18|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 732
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +3
Query: 489 PYPLPTGTYAPVISAEKAYHEQL 557
P P P GT +P+ S+E+A H+Q+
Sbjct: 481 PSP-PKGTKSPMASSEQAQHQQV 502
>SPAC6G9.04 |mug79||meiotically upregulated gene
Mug79|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.4 bits (53), Expect = 8.7
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = -2
Query: 477 QVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDGFIVDHE 304
Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E
Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,194,726
Number of Sequences: 5004
Number of extensions: 66233
Number of successful extensions: 224
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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