Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30258
(664 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 140 2e-34
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 139 3e-34
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 139 3e-34
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 99 5e-22
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 90 3e-19
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 70 3e-13
SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 32 0.085
SPAC1420.04c |cox1101|cox11, SPAPB17E12.01c, cox11|fusion cytoch... 26 4.2
SPAC19B12.13 |cox1102|cox11, cox11-b, cox11, SPAPB8E5.01|fusion ... 26 4.2
SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synth... 26 5.6
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 140 bits (338), Expect = 2e-34
Identities = 67/88 (76%), Positives = 78/88 (88%)
Frame = +2
Query: 2 GHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVN 181
GHRDFIKNMITGTSQADCAVLI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VN
Sbjct: 94 GHRDFIKNMITGTSQADCAVLIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVN 153
Query: 182 KMDSTEPPYSEPRFEEIKKEVSSYTRRL 265
KMD+T +S+ RFEEI KE S++ +++
Sbjct: 154 KMDTT--GWSQARFEEIVKETSNFIKKV 179
Score = 118 bits (284), Expect = 8e-28
Identities = 58/121 (47%), Positives = 72/121 (59%), Gaps = 2/121 (1%)
Frame = +1
Query: 256 KKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKERQADGKCXXXXXXXXXXX 435
KK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K GK
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPP 236
Query: 436 XXXXTSPCVFPCKTYTK--SVVLVPCRRQIETGVLKXGTIVVFAPANITTEVKSVEMHHE 609
P P + K + VP R +ETGV+K G IV FAPA +TTEVKSVEMHHE
Sbjct: 237 ARPTDKPLRLPLQDVYKIGGIGTVPVGR-VETGVIKPGMIVTFAPAGVTTEVKSVEMHHE 295
Query: 610 A 612
+
Sbjct: 296 S 296
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 139 bits (337), Expect = 3e-34
Identities = 66/88 (75%), Positives = 78/88 (88%)
Frame = +2
Query: 2 GHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVN 181
GHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VN
Sbjct: 94 GHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVN 153
Query: 182 KMDSTEPPYSEPRFEEIKKEVSSYTRRL 265
KMD+T +S+ RFEEI KE S++ +++
Sbjct: 154 KMDTT--GWSQARFEEIVKETSNFIKKV 179
Score = 118 bits (284), Expect = 8e-28
Identities = 58/121 (47%), Positives = 72/121 (59%), Gaps = 2/121 (1%)
Frame = +1
Query: 256 KKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKERQADGKCXXXXXXXXXXX 435
KK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K GK
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPP 236
Query: 436 XXXXTSPCVFPCKTYTK--SVVLVPCRRQIETGVLKXGTIVVFAPANITTEVKSVEMHHE 609
P P + K + VP R +ETGV+K G IV FAPA +TTEVKSVEMHHE
Sbjct: 237 ARPTDKPLRLPLQDVYKIGGIGTVPVGR-VETGVIKPGMIVTFAPAGVTTEVKSVEMHHE 295
Query: 610 A 612
+
Sbjct: 296 S 296
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 139 bits (337), Expect = 3e-34
Identities = 66/88 (75%), Positives = 78/88 (88%)
Frame = +2
Query: 2 GHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVN 181
GHRDFIKNMITGTSQADCA+LI+ GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VN
Sbjct: 94 GHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVN 153
Query: 182 KMDSTEPPYSEPRFEEIKKEVSSYTRRL 265
KMD+T +S+ RFEEI KE S++ +++
Sbjct: 154 KMDTT--GWSQARFEEIVKETSNFIKKV 179
Score = 118 bits (284), Expect = 8e-28
Identities = 58/121 (47%), Positives = 72/121 (59%), Gaps = 2/121 (1%)
Frame = +1
Query: 256 KKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKERQADGKCXXXXXXXXXXX 435
KK+G+NP V FVP+SG+ GDNM+EP+T MPW++GWQ E K GK
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPP 236
Query: 436 XXXXTSPCVFPCKTYTK--SVVLVPCRRQIETGVLKXGTIVVFAPANITTEVKSVEMHHE 609
P P + K + VP R +ETGV+K G IV FAPA +TTEVKSVEMHHE
Sbjct: 237 ARPTDKPLRLPLQDVYKIGGIGTVPVGR-VETGVIKPGMIVTFAPAGVTTEVKSVEMHHE 295
Query: 610 A 612
+
Sbjct: 296 S 296
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 99.1 bits (236), Expect = 5e-22
Identities = 44/89 (49%), Positives = 62/89 (69%)
Frame = +2
Query: 2 GHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVN 181
GH+ ++ NMI G SQAD VL+++A GEFEAG + GQTREHA+LA T G+ L+V +N
Sbjct: 325 GHKGYVTNMINGASQADIGVLVISARRGEFEAGFERGGQTREHAVLARTQGINHLVVVIN 384
Query: 182 KMDSTEPPYSEPRFEEIKKEVSSYTRRLA 268
KMD +SE R++E ++S + RR+A
Sbjct: 385 KMDEPSVQWSEERYKECVDKLSMFLRRVA 413
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 89.8 bits (213), Expect = 3e-19
Identities = 44/84 (52%), Positives = 55/84 (65%)
Frame = +2
Query: 2 GHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVN 181
GHRDFI MI G S AD AVL+V + FE G +NGQTREHA L LG+ +++V VN
Sbjct: 264 GHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVN 323
Query: 182 KMDSTEPPYSEPRFEEIKKEVSSY 253
K+D +SE RF+EIK VS +
Sbjct: 324 KLDLMS--WSEDRFQEIKNIVSDF 345
Score = 29.9 bits (64), Expect = 0.34
Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Frame = +1
Query: 256 KKIGYNPAAVAFVPISGWHGDNML--EPSTKMPWFKG 360
K +G+ + V FVPIS G N++ + S W+KG
Sbjct: 348 KMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG 384
Score = 25.8 bits (54), Expect = 5.6
Identities = 10/16 (62%), Positives = 13/16 (81%)
Frame = +3
Query: 438 RPTDKPLRLSLQDVYQ 485
+P KPLRLS+ DVY+
Sbjct: 399 KPYRKPLRLSIDDVYR 414
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 69.7 bits (163), Expect = 3e-13
Identities = 37/84 (44%), Positives = 53/84 (63%)
Frame = +2
Query: 2 GHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVN 181
GH D+IKNMITG + D A+++V+A G+ QTREH LLA +GVKQ++V +N
Sbjct: 125 GHADYIKNMITGAATMDGAIIVVSATDGQMP-------QTREHLLLARQVGVKQIVVYIN 177
Query: 182 KMDSTEPPYSEPRFEEIKKEVSSY 253
K+D EP E E+++ +S Y
Sbjct: 178 KVDMVEPDMIELVEMEMRELLSEY 201
Score = 28.3 bits (60), Expect = 1.0
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +3
Query: 438 RPTDKPLRLSLQDVYQIGGIGTV 506
R TD P ++++DV+ I G GTV
Sbjct: 247 RKTDVPFLMAIEDVFSISGRGTV 269
>SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma
subunit|Schizosaccharomyces pombe|chr 2|||Manual
Length = 446
Score = 31.9 bits (69), Expect = 0.085
Identities = 20/79 (25%), Positives = 34/79 (43%)
Frame = +2
Query: 2 GHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVN 181
GH + M+ G + D A+L++A QT EH + +K +I+ N
Sbjct: 117 GHDILMATMLNGAAVMDAALLLIAGNES------CPQPQTSEHLAAIEIMQLKHIIILQN 170
Query: 182 KMDSTEPPYSEPRFEEIKK 238
K+D +E ++ I K
Sbjct: 171 KVDLIRESAAEEHYQSILK 189
>SPAC1420.04c |cox1101|cox11, SPAPB17E12.01c, cox11|fusion
cytochrome c oxidase assembly protein Cox1101,
mitochondrial ribosomal protein
Rsm22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 753
Score = 26.2 bits (55), Expect = 4.2
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 227 EIKKEVSSYTRRLATTQLLSLSCPFLDGTETT 322
+IKK ++ + LAT L LS PF D + T
Sbjct: 104 QIKKFINDIKKDLATESQLPLSAPFKDESTRT 135
>SPAC19B12.13 |cox1102|cox11, cox11-b, cox11, SPAPB8E5.01|fusion
cytochrome c oxidase assembly protein Cox1102,
mitochondrial ribosomal protein
Rsm2202|Schizosaccharomyces pombe|chr 1|||Manual
Length = 753
Score = 26.2 bits (55), Expect = 4.2
Identities = 13/32 (40%), Positives = 18/32 (56%)
Frame = +2
Query: 227 EIKKEVSSYTRRLATTQLLSLSCPFLDGTETT 322
+IKK ++ + LAT L LS PF D + T
Sbjct: 104 QIKKFINDIKKDLATESQLPLSAPFKDESTRT 135
>SPAC6F12.10c |ade3|min11|phosphoribosylformylglycinamidine synthase
Ade3 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1323
Score = 25.8 bits (54), Expect = 5.6
Identities = 20/72 (27%), Positives = 31/72 (43%)
Frame = -3
Query: 503 GTNTTDLVYVLQGKTQGLVSGAGSGRMASRASMRHFPSACLSLRSTCHPLNQGILVEGSN 324
G NT + + + + LVS A S RMA + + +A + P I + +
Sbjct: 703 GINTGEALAMGEKPISALVSAAASARMAVAECIMNLVAASI-------PALDRIRLSANW 755
Query: 323 MLSPCHPEMGTK 288
M +P HP G K
Sbjct: 756 MAAPSHPGEGAK 767
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,943,635
Number of Sequences: 5004
Number of extensions: 63532
Number of successful extensions: 207
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 186
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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