SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30257
         (747 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit...   105   5e-24
SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu...    53   5e-08
SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ...    49   8e-07
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...    49   8e-07
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni...    45   1e-05
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit...    43   5e-05
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C...    40   5e-04
SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C...    38   0.002
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|...    29   0.70 
SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch...    29   0.70 
SPAC1565.07c |||TATA binding protein interacting protein |Schizo...    28   1.2  
SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po...    27   2.8  
SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein...    27   3.8  
SPAC139.01c ||SPAC955.02c|nuclease, XP-G family|Schizosaccharomy...    27   3.8  
SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz...    26   5.0  
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   6.6  
SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomy...    26   6.6  
SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa...    25   8.7  
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar...    25   8.7  

>SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit
           Cct8 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 546

 Score =  105 bits (253), Expect = 5e-24
 Identities = 60/163 (36%), Positives = 88/163 (53%), Gaps = 1/163 (0%)
 Frame = +1

Query: 250 KGIVAAIMSKQYGHEQFISELVTKACVAILP-EKTTFNVDNVRVCKILGAGLLQSEVLSG 426
           K I   I SKQYG+E F+S+LV KA + +LP + + FNVDN+RV KI+G+ L  S+V+ G
Sbjct: 161 KAIRTCISSKQYGNEDFLSDLVAKAILTVLPKDPSKFNVDNIRVVKIMGSSLYNSQVVKG 220

Query: 427 MVFRREVEGDVSSAKNAKIAVYSCPIDKXXXXXXXXXXXXXXMNY*TSVKEKNLYLKSRL 606
           MVF RE EG V+ +K AK+AV+SCP+D                      K +   ++S +
Sbjct: 221 MVFPREPEGTVTRSKEAKVAVFSCPLDISQTETKGTVLLHNAQEMLDFSKGEENLIESHI 280

Query: 607 KIFPTXXXXXXXXXXXXEIWALHFLNKYHYYGLSRLNSKFDIR 735
           K                    LH+LN++    + R+ SKF++R
Sbjct: 281 KEIYDAGVRVVVTSGNVNDLVLHYLNRFEIL-VIRVPSKFELR 322



 Score = 76.2 bits (179), Expect = 5e-15
 Identities = 44/116 (37%), Positives = 67/116 (57%), Gaps = 2/116 (1%)
 Frame = +2

Query: 5   HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184
           HPAAKL+V A+Q Q+ E+GD  NFV+V +G          R+G+T  EIA GYE AL   
Sbjct: 79  HPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLTPLEIAKGYEMALSHT 138

Query: 185 LEILPSLICNEIKDTKNIKEVTKVLLLLSCLNN-MVMNNSFLS-LSRKPVLQYYQK 346
           +E+L  +  ++I+  ++ KE+ K   + +C+++    N  FLS L  K +L    K
Sbjct: 139 MEVLEEICADKIETVESEKELIKA--IRTCISSKQYGNEDFLSDLVAKAILTVLPK 192


>SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit
           Cct5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 546

 Score = 52.8 bits (121), Expect = 5e-08
 Identities = 32/90 (35%), Positives = 46/90 (51%), Gaps = 3/90 (3%)
 Frame = +2

Query: 2   EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALD- 178
           EH  AKL+V  S+ QD E+GDGT  V+VL+G            G+    IADGYE+A   
Sbjct: 86  EHQIAKLLVQLSKSQDDEIGDGTTGVVVLAGALLEQAEALIDKGIHPIRIADGYEKACQV 145

Query: 179 --KCLEILPSLICNEIKDTKNIKEVTKVLL 262
             K L+ +  ++    ++T N+    K  L
Sbjct: 146 AVKHLDAISDVVDFSPENTTNLFRSAKTSL 175


>SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit
           Cct7|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 558

 Score = 48.8 bits (111), Expect = 8e-07
 Identities = 33/124 (26%), Positives = 60/124 (48%), Gaps = 3/124 (2%)
 Frame = +2

Query: 5   HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184
           HPAAK +V  ++ QDAEVGDGT  V+V +G            GV++  I  GY +A    
Sbjct: 77  HPAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVEDGVSSHLIIRGYRKAAQLA 136

Query: 185 LEILPSLICN-EIKDTKNIKEVTKVLLLLSCLNNMVMNNS--FLSLSRKPVLQYYQKKLH 355
           +  +  +  + ++ D   ++++       +  + ++ +NS  F  +    VL   Q+ L+
Sbjct: 137 VNKIKEIAIHLDLSDEGKLRDLLTKCASTAMNSKLIRSNSTFFTKMVVDAVLTLDQEDLN 196

Query: 356 LMLI 367
             +I
Sbjct: 197 ENMI 200


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score = 48.8 bits (111), Expect = 8e-07
 Identities = 24/79 (30%), Positives = 44/79 (55%), Gaps = 1/79 (1%)
 Frame = +2

Query: 5   HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184
           HPAAK++V  S  QD E GDGT  V++L+G          + G+  + IA+ ++RA    
Sbjct: 72  HPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFT 131

Query: 185 LEIL-PSLICNEIKDTKNI 238
           ++ +  + +  E+ D +++
Sbjct: 132 VDCMKENALAIELSDRESL 150


>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
           Cct1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 556

 Score = 45.2 bits (102), Expect = 1e-05
 Identities = 20/69 (28%), Positives = 37/69 (53%)
 Frame = +2

Query: 2   EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDK 181
           EHPA K++V  +Q QD EVGDGT  V++++           +  +  + I  GY  A+ +
Sbjct: 73  EHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNKIHPTTIITGYRLAIRE 132

Query: 182 CLEILPSLI 208
            ++ +  ++
Sbjct: 133 AVKFMTDVL 141


>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
           Cct3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 528

 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 22/67 (32%), Positives = 35/67 (52%)
 Frame = +2

Query: 5   HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184
           HPAAK M+  ++ QD EVGDGT  VI+L+G             +    +   +++AL+  
Sbjct: 72  HPAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLDRKIHPVVMIRSFKQALEDA 131

Query: 185 LEILPSL 205
           L I+  +
Sbjct: 132 LSIIDEI 138


>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
           Cct6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 535

 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 26/87 (29%), Positives = 39/87 (44%)
 Frame = +2

Query: 2   EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDK 181
           ++P A  +  A+  QD   GDGT  V +L G          R G+  S I+DG+  A ++
Sbjct: 68  QNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREGLHPSLISDGFNLAKNE 127

Query: 182 CLEILPSLICNEIKDTKNIKEVTKVLL 262
            L  L S   +   D + +  V K  L
Sbjct: 128 ALTFLDSFKTDFEVDREVLLNVAKTSL 154


>SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit
           Cct2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 527

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 21/65 (32%), Positives = 31/65 (47%)
 Frame = +2

Query: 2   EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDK 181
           ++ AAK++V  S++QD EVGDGT  V V +              +    I DGY  A   
Sbjct: 73  DNAAAKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIHPQVIIDGYRIATKT 132

Query: 182 CLEIL 196
            ++ L
Sbjct: 133 AIDAL 137



 Score = 30.3 bits (65), Expect = 0.30
 Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
 Frame = +1

Query: 331 AILPEKTTFNVDNVRVCKILGAGLLQSEVLSGMVFRREVEGDVSSA-KNAKIAVYSCPID 507
           A+L  K + N+DN+++ KILG  L  S +  G +  + +  +     +NA I + +  +D
Sbjct: 182 AVLRLKGSTNLDNIQIIKILGGKLDDSFLDEGFILNKTIGVNCPKVMENANILIANTAMD 241


>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 732

 Score = 29.1 bits (62), Expect = 0.70
 Identities = 15/44 (34%), Positives = 27/44 (61%)
 Frame = +2

Query: 185 LEILPSLICNEIKDTKNIKEVTKVLLLLSCLNNMVMNNSFLSLS 316
           + +LP LI +  K+  ++ +     LL  CL+N+ ++NS +SLS
Sbjct: 629 VSVLP-LIKDSAKNLLSVTDPESEYLLKQCLSNISLSNSVISLS 671


>SPBC21D10.06c |map4||cell agglutination protein
           Map4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 948

 Score = 29.1 bits (62), Expect = 0.70
 Identities = 17/54 (31%), Positives = 29/54 (53%)
 Frame = -2

Query: 452 PSTSRLNTIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSSEMNCS 291
           P TS +   P +TSD  +P PR   + T +++   +S S ++   +TSS  + S
Sbjct: 117 PYTSSVACFPYATSDAPNPIPR-GDSATSTSIAPTYSASDSSATTITSSSPSTS 169


>SPAC1565.07c |||TATA binding protein interacting protein
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1220

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 19/76 (25%), Positives = 35/76 (46%), Gaps = 3/76 (3%)
 Frame = +2

Query: 221  KDTKNIKEVTKV---LLLLSCLNNMVMNNSFLSLSRKPVLQYYQKKLHLMLIMLEFAKF* 391
            KD +NIK+ +      LL  CL  ++ N S     +  +L   +   H++L +  F    
Sbjct: 933  KDIENIKDFSTSPFRTLLSECLGLLICNESSSLYYKLELLSSSEASNHMLLSLSVFRFSL 992

Query: 392  VLDCCNLRYFLEWYLD 439
             LDC  L+ + + + +
Sbjct: 993  TLDCPKLKAYEKQFFE 1008


>SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1564

 Score = 27.1 bits (57), Expect = 2.8
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = +3

Query: 117 NYSAWE*LPVKLLMDMREHLTNVWKSFLLS 206
           NYS WE + + L   +R  L N  KSF+L+
Sbjct: 559 NYSLWEAVGISLNYIVRNGLINSHKSFVLT 588


>SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 670

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 14/42 (33%), Positives = 21/42 (50%)
 Frame = -2

Query: 431 TIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSS 306
           ++P S S   S +     TLT S  +V ++G+    A  TSS
Sbjct: 304 SVPSSVSSFTSSSSSYTTTLTASNTSVTYTGTGTGSATFTSS 345



 Score = 26.2 bits (55), Expect = 5.0
 Identities = 15/42 (35%), Positives = 19/42 (45%)
 Frame = -2

Query: 431 TIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSS 306
           ++P S S   S       TLT S   V F+G+    A  TSS
Sbjct: 412 SVPSSVSSFTSSNSSYTTTLTASNTTVTFTGTGTGSATFTSS 453


>SPAC139.01c ||SPAC955.02c|nuclease, XP-G family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 802

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 16/39 (41%), Positives = 22/39 (56%)
 Frame = +2

Query: 320 KPVLQYYQKKLHLMLIMLEFAKF*VLDCCNLRYFLEWYL 436
           KP L Y  ++ HL  +  +  KF  LD CN+ YF+  YL
Sbjct: 140 KPTLLYTNQRDHLDRLC-DQVKF-YLDQCNVEYFVAPYL 176


>SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase
           Ssp2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 576

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 20/70 (28%), Positives = 34/70 (48%)
 Frame = +2

Query: 143 SEIADGYERALDKCLEILPSLICNEIKDTKNIKEVTKVLLLLSCLNNMVMNNSFLSLSRK 322
           S++ +    + D  +E L S   NE+K+  N+    +V+   S L+     +SFLS+S  
Sbjct: 310 SKLGEAMGFSEDYIVEALRSDENNEVKEAYNLLHENQVIQEKSHLSKSKRVDSFLSVS-P 368

Query: 323 PVLQYYQKKL 352
           P    Y  +L
Sbjct: 369 PAFSEYTSEL 378


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 19/59 (32%), Positives = 31/59 (52%)
 Frame = -2

Query: 467 AEETSPSTSRLNTIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSSEMNCS 291
           A  TS S+S L++     S  +SP+     TLT S+L+     S ++ +  TSS ++ S
Sbjct: 200 AAPTSTSSSYLSS-SSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSS 257


>SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 385

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 15/49 (30%), Positives = 29/49 (59%)
 Frame = -2

Query: 452 PSTSRLNTIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSS 306
           PS+S+  +   ST+D    +  I  T++ + ++ ++SG+  T  FV+SS
Sbjct: 108 PSSSQTISASSSTTDNVIVSSSISSTVSSTPVSTIYSGTSGT-TFVSSS 155


>SPAC19A8.08 |upf2||nonsense-mediated decay protein
           Upf2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1049

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 16/56 (28%), Positives = 27/56 (48%)
 Frame = +1

Query: 217 DKRHKKH*RSYKGIVAAIMSKQYGHEQFISELVTKACVAILPEKTTFNVDNVRVCK 384
           D   KK+    K   +++ S+ Y  + FI E+ T +    +PE T   V+ +  CK
Sbjct: 38  DSSLKKNTAFMKRCKSSLTSENY--DSFIKEIKTLSLKKFIPEITAAIVEGMMKCK 91


>SPAC25B8.04c |||mitochondrial splicing suppressor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 378

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 10/33 (30%), Positives = 20/33 (60%)
 Frame = -1

Query: 234 FFVSFISLQMREGRISRHLSSALSYPSAISLVV 136
           FF +    + +  R +RH++S L+YP +I  ++
Sbjct: 120 FFYTRDFPKFQSSRTARHITSLLTYPMSIGAIL 152


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,915,988
Number of Sequences: 5004
Number of extensions: 57825
Number of successful extensions: 212
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -