BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30257 (747 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit... 105 5e-24 SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subu... 53 5e-08 SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit ... 49 8e-07 SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ... 49 8e-07 SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 45 1e-05 SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 43 5e-05 SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 40 5e-04 SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit C... 38 0.002 SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|... 29 0.70 SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosacch... 29 0.70 SPAC1565.07c |||TATA binding protein interacting protein |Schizo... 28 1.2 SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po... 27 2.8 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 27 3.8 SPAC139.01c ||SPAC955.02c|nuclease, XP-G family|Schizosaccharomy... 27 3.8 SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 26 5.0 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.6 SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomy... 26 6.6 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 25 8.7 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 25 8.7 >SPBC337.05c |cct8||chaperonin-containing T-complex theta subunit Cct8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 546 Score = 105 bits (253), Expect = 5e-24 Identities = 60/163 (36%), Positives = 88/163 (53%), Gaps = 1/163 (0%) Frame = +1 Query: 250 KGIVAAIMSKQYGHEQFISELVTKACVAILP-EKTTFNVDNVRVCKILGAGLLQSEVLSG 426 K I I SKQYG+E F+S+LV KA + +LP + + FNVDN+RV KI+G+ L S+V+ G Sbjct: 161 KAIRTCISSKQYGNEDFLSDLVAKAILTVLPKDPSKFNVDNIRVVKIMGSSLYNSQVVKG 220 Query: 427 MVFRREVEGDVSSAKNAKIAVYSCPIDKXXXXXXXXXXXXXXMNY*TSVKEKNLYLKSRL 606 MVF RE EG V+ +K AK+AV+SCP+D K + ++S + Sbjct: 221 MVFPREPEGTVTRSKEAKVAVFSCPLDISQTETKGTVLLHNAQEMLDFSKGEENLIESHI 280 Query: 607 KIFPTXXXXXXXXXXXXEIWALHFLNKYHYYGLSRLNSKFDIR 735 K LH+LN++ + R+ SKF++R Sbjct: 281 KEIYDAGVRVVVTSGNVNDLVLHYLNRFEIL-VIRVPSKFELR 322 Score = 76.2 bits (179), Expect = 5e-15 Identities = 44/116 (37%), Positives = 67/116 (57%), Gaps = 2/116 (1%) Frame = +2 Query: 5 HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184 HPAAKL+V A+Q Q+ E+GD NFV+V +G R+G+T EIA GYE AL Sbjct: 79 HPAAKLVVDATQQQENELGDAANFVVVFTGELLAKAENMIRMGLTPLEIAKGYEMALSHT 138 Query: 185 LEILPSLICNEIKDTKNIKEVTKVLLLLSCLNN-MVMNNSFLS-LSRKPVLQYYQK 346 +E+L + ++I+ ++ KE+ K + +C+++ N FLS L K +L K Sbjct: 139 MEVLEEICADKIETVESEKELIKA--IRTCISSKQYGNEDFLSDLVAKAILTVLPK 192 >SPAC1420.02c |cct5||chaperonin-containing T-complex epsilon subunit Cct5|Schizosaccharomyces pombe|chr 1|||Manual Length = 546 Score = 52.8 bits (121), Expect = 5e-08 Identities = 32/90 (35%), Positives = 46/90 (51%), Gaps = 3/90 (3%) Frame = +2 Query: 2 EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALD- 178 EH AKL+V S+ QD E+GDGT V+VL+G G+ IADGYE+A Sbjct: 86 EHQIAKLLVQLSKSQDDEIGDGTTGVVVLAGALLEQAEALIDKGIHPIRIADGYEKACQV 145 Query: 179 --KCLEILPSLICNEIKDTKNIKEVTKVLL 262 K L+ + ++ ++T N+ K L Sbjct: 146 AVKHLDAISDVVDFSPENTTNLFRSAKTSL 175 >SPBC25H2.12c |cct7||chaperonin-containing T-complex eta subunit Cct7|Schizosaccharomyces pombe|chr 2|||Manual Length = 558 Score = 48.8 bits (111), Expect = 8e-07 Identities = 33/124 (26%), Positives = 60/124 (48%), Gaps = 3/124 (2%) Frame = +2 Query: 5 HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184 HPAAK +V ++ QDAEVGDGT V+V +G GV++ I GY +A Sbjct: 77 HPAAKTLVDIARAQDAEVGDGTTSVVVFAGELLREARTFVEDGVSSHLIIRGYRKAAQLA 136 Query: 185 LEILPSLICN-EIKDTKNIKEVTKVLLLLSCLNNMVMNNS--FLSLSRKPVLQYYQKKLH 355 + + + + ++ D ++++ + + ++ +NS F + VL Q+ L+ Sbjct: 137 VNKIKEIAIHLDLSDEGKLRDLLTKCASTAMNSKLIRSNSTFFTKMVVDAVLTLDQEDLN 196 Query: 356 LMLI 367 +I Sbjct: 197 ENMI 200 >SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit Cct4|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 48.8 bits (111), Expect = 8e-07 Identities = 24/79 (30%), Positives = 44/79 (55%), Gaps = 1/79 (1%) Frame = +2 Query: 5 HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184 HPAAK++V S QD E GDGT V++L+G + G+ + IA+ ++RA Sbjct: 72 HPAAKMLVDLSAAQDVEAGDGTTSVVILAGSMLACAEKLLKKGIHPTVIAESFQRAAGFT 131 Query: 185 LEIL-PSLICNEIKDTKNI 238 ++ + + + E+ D +++ Sbjct: 132 VDCMKENALAIELSDRESL 150 >SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit Cct1|Schizosaccharomyces pombe|chr 2|||Manual Length = 556 Score = 45.2 bits (102), Expect = 1e-05 Identities = 20/69 (28%), Positives = 37/69 (53%) Frame = +2 Query: 2 EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDK 181 EHPA K++V +Q QD EVGDGT V++++ + + + I GY A+ + Sbjct: 73 EHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNKIHPTTIITGYRLAIRE 132 Query: 182 CLEILPSLI 208 ++ + ++ Sbjct: 133 AVKFMTDVL 141 >SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit Cct3|Schizosaccharomyces pombe|chr 2|||Manual Length = 528 Score = 42.7 bits (96), Expect = 5e-05 Identities = 22/67 (32%), Positives = 35/67 (52%) Frame = +2 Query: 5 HPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDKC 184 HPAAK M+ ++ QD EVGDGT VI+L+G + + +++AL+ Sbjct: 72 HPAAKSMIELARTQDEEVGDGTTSVIILAGEILAAASPLLDRKIHPVVMIRSFKQALEDA 131 Query: 185 LEILPSL 205 L I+ + Sbjct: 132 LSIIDEI 138 >SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit Cct6|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 39.5 bits (88), Expect = 5e-04 Identities = 26/87 (29%), Positives = 39/87 (44%) Frame = +2 Query: 2 EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDK 181 ++P A + A+ QD GDGT V +L G R G+ S I+DG+ A ++ Sbjct: 68 QNPTASCIAKAATAQDDATGDGTTSVCLLVGELLKQAELYIREGLHPSLISDGFNLAKNE 127 Query: 182 CLEILPSLICNEIKDTKNIKEVTKVLL 262 L L S + D + + V K L Sbjct: 128 ALTFLDSFKTDFEVDREVLLNVAKTSL 154 >SPAC1D4.04 |cct2||chaperonin-containing T-complex beta subunit Cct2|Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 37.5 bits (83), Expect = 0.002 Identities = 21/65 (32%), Positives = 31/65 (47%) Frame = +2 Query: 2 EHPAAKLMVLASQMQDAEVGDGTNFVIVLSGXXXXXXXXXXRLGVTTSEIADGYERALDK 181 ++ AAK++V S++QD EVGDGT V V + + I DGY A Sbjct: 73 DNAAAKVLVNISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIHPQVIIDGYRIATKT 132 Query: 182 CLEIL 196 ++ L Sbjct: 133 AIDAL 137 Score = 30.3 bits (65), Expect = 0.30 Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 1/60 (1%) Frame = +1 Query: 331 AILPEKTTFNVDNVRVCKILGAGLLQSEVLSGMVFRREVEGDVSSA-KNAKIAVYSCPID 507 A+L K + N+DN+++ KILG L S + G + + + + +NA I + + +D Sbjct: 182 AVLRLKGSTNLDNIQIIKILGGKLDDSFLDEGFILNKTIGVNCPKVMENANILIANTAMD 241 >SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr 1|||Manual Length = 732 Score = 29.1 bits (62), Expect = 0.70 Identities = 15/44 (34%), Positives = 27/44 (61%) Frame = +2 Query: 185 LEILPSLICNEIKDTKNIKEVTKVLLLLSCLNNMVMNNSFLSLS 316 + +LP LI + K+ ++ + LL CL+N+ ++NS +SLS Sbjct: 629 VSVLP-LIKDSAKNLLSVTDPESEYLLKQCLSNISLSNSVISLS 671 >SPBC21D10.06c |map4||cell agglutination protein Map4|Schizosaccharomyces pombe|chr 2|||Manual Length = 948 Score = 29.1 bits (62), Expect = 0.70 Identities = 17/54 (31%), Positives = 29/54 (53%) Frame = -2 Query: 452 PSTSRLNTIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSSEMNCS 291 P TS + P +TSD +P PR + T +++ +S S ++ +TSS + S Sbjct: 117 PYTSSVACFPYATSDAPNPIPR-GDSATSTSIAPTYSASDSSATTITSSSPSTS 169 >SPAC1565.07c |||TATA binding protein interacting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 1220 Score = 28.3 bits (60), Expect = 1.2 Identities = 19/76 (25%), Positives = 35/76 (46%), Gaps = 3/76 (3%) Frame = +2 Query: 221 KDTKNIKEVTKV---LLLLSCLNNMVMNNSFLSLSRKPVLQYYQKKLHLMLIMLEFAKF* 391 KD +NIK+ + LL CL ++ N S + +L + H++L + F Sbjct: 933 KDIENIKDFSTSPFRTLLSECLGLLICNESSSLYYKLELLSSSEASNHMLLSLSVFRFSL 992 Query: 392 VLDCCNLRYFLEWYLD 439 LDC L+ + + + + Sbjct: 993 TLDCPKLKAYEKQFFE 1008 >SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces pombe|chr 1|||Manual Length = 1564 Score = 27.1 bits (57), Expect = 2.8 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = +3 Query: 117 NYSAWE*LPVKLLMDMREHLTNVWKSFLLS 206 NYS WE + + L +R L N KSF+L+ Sbjct: 559 NYSLWEAVGISLNYIVRNGLINSHKSFVLT 588 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 26.6 bits (56), Expect = 3.8 Identities = 14/42 (33%), Positives = 21/42 (50%) Frame = -2 Query: 431 TIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSS 306 ++P S S S + TLT S +V ++G+ A TSS Sbjct: 304 SVPSSVSSFTSSSSSYTTTLTASNTSVTYTGTGTGSATFTSS 345 Score = 26.2 bits (55), Expect = 5.0 Identities = 15/42 (35%), Positives = 19/42 (45%) Frame = -2 Query: 431 TIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSS 306 ++P S S S TLT S V F+G+ A TSS Sbjct: 412 SVPSSVSSFTSSNSSYTTTLTASNTTVTFTGTGTGSATFTSS 453 >SPAC139.01c ||SPAC955.02c|nuclease, XP-G family|Schizosaccharomyces pombe|chr 1|||Manual Length = 802 Score = 26.6 bits (56), Expect = 3.8 Identities = 16/39 (41%), Positives = 22/39 (56%) Frame = +2 Query: 320 KPVLQYYQKKLHLMLIMLEFAKF*VLDCCNLRYFLEWYL 436 KP L Y ++ HL + + KF LD CN+ YF+ YL Sbjct: 140 KPTLLYTNQRDHLDRLC-DQVKF-YLDQCNVEYFVAPYL 176 >SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schizosaccharomyces pombe|chr 3|||Manual Length = 576 Score = 26.2 bits (55), Expect = 5.0 Identities = 20/70 (28%), Positives = 34/70 (48%) Frame = +2 Query: 143 SEIADGYERALDKCLEILPSLICNEIKDTKNIKEVTKVLLLLSCLNNMVMNNSFLSLSRK 322 S++ + + D +E L S NE+K+ N+ +V+ S L+ +SFLS+S Sbjct: 310 SKLGEAMGFSEDYIVEALRSDENNEVKEAYNLLHENQVIQEKSHLSKSKRVDSFLSVS-P 368 Query: 323 PVLQYYQKKL 352 P Y +L Sbjct: 369 PAFSEYTSEL 378 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.8 bits (54), Expect = 6.6 Identities = 19/59 (32%), Positives = 31/59 (52%) Frame = -2 Query: 467 AEETSPSTSRLNTIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSSEMNCS 291 A TS S+S L++ S +SP+ TLT S+L+ S ++ + TSS ++ S Sbjct: 200 AAPTSTSSSYLSS-SSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSSSTSSSLSSS 257 >SPAC1F8.06 |fta5|sma5|Sim4 and Mal2 associated |Schizosaccharomyces pombe|chr 1|||Manual Length = 385 Score = 25.8 bits (54), Expect = 6.6 Identities = 15/49 (30%), Positives = 29/49 (59%) Frame = -2 Query: 452 PSTSRLNTIPESTSDCNSPAPRILQTLTLSTLNVVFSGSIATQAFVTSS 306 PS+S+ + ST+D + I T++ + ++ ++SG+ T FV+SS Sbjct: 108 PSSSQTISASSSTTDNVIVSSSISSTVSSTPVSTIYSGTSGT-TFVSSS 155 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 25.4 bits (53), Expect = 8.7 Identities = 16/56 (28%), Positives = 27/56 (48%) Frame = +1 Query: 217 DKRHKKH*RSYKGIVAAIMSKQYGHEQFISELVTKACVAILPEKTTFNVDNVRVCK 384 D KK+ K +++ S+ Y + FI E+ T + +PE T V+ + CK Sbjct: 38 DSSLKKNTAFMKRCKSSLTSENY--DSFIKEIKTLSLKKFIPEITAAIVEGMMKCK 91 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 25.4 bits (53), Expect = 8.7 Identities = 10/33 (30%), Positives = 20/33 (60%) Frame = -1 Query: 234 FFVSFISLQMREGRISRHLSSALSYPSAISLVV 136 FF + + + R +RH++S L+YP +I ++ Sbjct: 120 FFYTRDFPKFQSSRTARHITSLLTYPMSIGAIL 152 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,915,988 Number of Sequences: 5004 Number of extensions: 57825 Number of successful extensions: 212 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 192 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 210 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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