Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30254
(781 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 160 2e-40
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 156 4e-39
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 86 6e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 67 2e-12
SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 34 0.026
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 32 0.11
SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizos... 31 0.14
SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar... 27 4.0
SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.3
SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces ... 26 7.0
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 25 9.2
SPAC6G9.04 |mug79||meiotically upregulated gene Mug79|Schizosacc... 25 9.2
SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr 1|||M... 25 9.2
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p... 25 9.2
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 160 bits (388), Expect = 2e-40
Identities = 74/91 (81%), Positives = 80/91 (87%)
Frame = +2
Query: 242 PRASVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIG 421
P VST+VVEPYNS+LTTH TL++SDC FMVDNEA YDICRRNLDIERPTY NLNRLI
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDICRRNLDIERPTYENLNRLIA 236
Query: 422 QIVSSITASLRFDGALNVDLTEFQTNLVPTP 514
Q+VSSITASLRF G+LNVDL EFQTNLVP P
Sbjct: 237 QVVSSITASLRFAGSLNVDLNEFQTNLVPYP 267
Score = 125 bits (301), Expect = 8e-30
Identities = 52/84 (61%), Positives = 69/84 (82%)
Frame = +3
Query: 3 ITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXL 182
+TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH L
Sbjct: 97 VTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGAL 156
Query: 183 LMERLSVDYGKKSKLEFAIYPAPQ 254
L+ERL+++YGKKS L+F++YPAPQ
Sbjct: 157 LLERLNMEYGKKSNLQFSVYPAPQ 180
Score = 107 bits (258), Expect = 1e-24
Identities = 54/91 (59%), Positives = 64/91 (70%), Gaps = 1/91 (1%)
Frame = +1
Query: 508 YPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGQVHGLAVCCTV 687
YPRIHFPLVTY+P++SA KA+HE SV EITN CFEP NQMVKCDPR G+ +A C
Sbjct: 266 YPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRTGRY--MATCLLY 323
Query: 688 VTS-*PRM*TRAIATIKTKRTIQFVDGVQTG 777
PR A+ +IK++RTIQFVD TG
Sbjct: 324 RGDVIPRDVQAAVTSIKSRRTIQFVDWCPTG 354
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 156 bits (378), Expect = 4e-39
Identities = 71/91 (78%), Positives = 80/91 (87%)
Frame = +2
Query: 242 PRASVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIG 421
P VST+VVEPYNS+LTTH TL+ +DC FMVDNE+ YDICRRNLDIERP+Y NLNRLI
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDICRRNLDIERPSYENLNRLIA 232
Query: 422 QIVSSITASLRFDGALNVDLTEFQTNLVPTP 514
Q+VSSITASLRF+G+LNVDL EFQTNLVP P
Sbjct: 233 QVVSSITASLRFEGSLNVDLAEFQTNLVPYP 263
Score = 123 bits (296), Expect = 3e-29
Identities = 53/84 (63%), Positives = 68/84 (80%)
Frame = +3
Query: 3 ITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXL 182
ITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH L
Sbjct: 93 ITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGAL 152
Query: 183 LMERLSVDYGKKSKLEFAIYPAPQ 254
L+ERL+++Y KKSKL+F++YPAPQ
Sbjct: 153 LLERLAMEYTKKSKLQFSVYPAPQ 176
Score = 111 bits (267), Expect = 1e-25
Identities = 57/91 (62%), Positives = 63/91 (69%), Gaps = 1/91 (1%)
Frame = +1
Query: 508 YPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGQVHGLAVCCTV 687
YPRIHFPLVTYAP++SA KA+HE SV EITN CFEP NQMVKCDPR G+ +A C
Sbjct: 262 YPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDPRAGRY--MATCLLY 319
Query: 688 VTS-*PRM*TRAIATIKTKRTIQFVDGVQTG 777
PR A+ TIK KRTIQFVD TG
Sbjct: 320 RGDVIPRDVQAAVTTIKAKRTIQFVDWCPTG 350
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 85.8 bits (203), Expect = 6e-18
Identities = 39/91 (42%), Positives = 55/91 (60%)
Frame = +2
Query: 242 PRASVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIG 421
P S VVEPYN+ L+ H +E+SD F +DNEA+ I L I+ P+Y +LN L+
Sbjct: 171 PAPKSSDTVVEPYNATLSMHQLVENSDETFCIDNEALSSIFANTLKIKSPSYDDLNHLVS 230
Query: 422 QIVSSITASLRFDGALNVDLTEFQTNLVPTP 514
+++ +T S RF G LN DL + N+VP P
Sbjct: 231 AVMAGVTTSFRFPGELNSDLRKLAVNMVPFP 261
Score = 64.9 bits (151), Expect = 1e-11
Identities = 30/84 (35%), Positives = 46/84 (54%)
Frame = +3
Query: 3 ITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXL 182
I G+ A N +A+GHYT G E+ D VLD +R+ A+ C LQGF + H L
Sbjct: 91 IYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTL 150
Query: 183 LMERLSVDYGKKSKLEFAIYPAPQ 254
L+ ++ +Y + F++ PAP+
Sbjct: 151 LLSKIREEYPDRMMATFSVAPAPK 174
Score = 52.0 bits (119), Expect = 9e-08
Identities = 27/87 (31%), Positives = 47/87 (54%)
Frame = +1
Query: 508 YPRIHFPLVTYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGQVHGLAVCCTV 687
+PR+HF +V +AP+ + + + +SV E+T F+ N MV DPRHG+ +A
Sbjct: 260 FPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADPRHGRYLTVAALFRG 319
Query: 688 VTS*PRM*TRAIATIKTKRTIQFVDGV 768
S + I +++TK + FV+ +
Sbjct: 320 KVSMKEV-DEQIRSVQTKNSAYFVEWI 345
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 67.3 bits (157), Expect = 2e-12
Identities = 31/88 (35%), Positives = 51/88 (57%)
Frame = +2
Query: 251 SVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIV 430
SVS VV+PYNS+L ++D ++DN A+ I L + PT+ N+L+ ++
Sbjct: 177 SVSDVVVQPYNSLLALKRLTLNADSVVVLDNAALAHIAADRLHTQNPTFHQQNQLVSTVM 236
Query: 431 SSITASLRFDGALNVDLTEFQTNLVPTP 514
S+ T +LR+ G +N DL +L+P+P
Sbjct: 237 SASTTTLRYPGYMNNDLVSIIASLIPSP 264
Score = 43.2 bits (97), Expect = 4e-05
Identities = 24/78 (30%), Positives = 39/78 (50%)
Frame = +3
Query: 21 AANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLS 200
A NN+A G Y+ + I + ++D I + AD L+GF + H L+ERL+
Sbjct: 100 AGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLN 158
Query: 201 VDYGKKSKLEFAIYPAPQ 254
Y KK ++++P Q
Sbjct: 159 DRYPKKIIQTYSVFPNSQ 176
Score = 27.5 bits (58), Expect = 2.3
Identities = 15/48 (31%), Positives = 22/48 (45%), Gaps = 3/48 (6%)
Frame = +1
Query: 511 PRIHFPLVTYAPVIS---AEKAYHEQLSVAEITNACFEPANQMVKCDP 645
PR HF L +Y P + E + +V ++ P NQMV +P
Sbjct: 264 PRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVSVNP 311
>SPBC1718.06 |msp1|mgm1|mitochondrial GTPase
Msp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 903
Score = 33.9 bits (74), Expect = 0.026
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -1
Query: 508 RHQVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDG 347
R + E + H ++ + Q+SSD ++ +D T VG+G AL I + D +DG
Sbjct: 165 RRVLQAERAKEHRSNSNDKQKSSDNDEDPNDTT--VGIGAALAASILSVDSVDG 216
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.9 bits (69), Expect = 0.11
Identities = 18/42 (42%), Positives = 24/42 (57%)
Frame = +2
Query: 503 VPTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTHASSPPTR 628
VP P S + T +SS+P P N+ PSP S + AS+ P R
Sbjct: 1239 VPAPSSEAPSVSTPRSSVPS-PHSNASPSPTSSSMASAAPAR 1279
>SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 721
Score = 31.5 bits (68), Expect = 0.14
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +3
Query: 606 MLRARQPDGEMRPPSWASTWACCMLYRGDVVTKDVNAGNRYHQN 737
+++ ++PDG SW +WA C Y T + + RY++N
Sbjct: 562 VVKMQRPDG-----SWYGSWAICFTYAAMFATGSLASAGRYYEN 600
>SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 598
Score = 26.6 bits (56), Expect = 4.0
Identities = 14/39 (35%), Positives = 20/39 (51%)
Frame = +2
Query: 467 LNVDLTEFQTNLVPTPVSTSHWSRTRQSSLPRRPTMNSF 583
L+VDL EF+T P PV T + T + + R +F
Sbjct: 341 LDVDL-EFETGAKPVPVQTEETTETLEDLIKNRIISKTF 378
>SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1158
Score = 26.2 bits (55), Expect = 5.3
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +2
Query: 545 QSSLPRRPTMNSFPSPRSQTHASSPPTR 628
+ SLPRRP+ +P S T ++ PP +
Sbjct: 738 RGSLPRRPSSALLTNPISITKSNPPPVK 765
>SPBC582.05c |brc1||BRCT domain protein Brc1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 878
Score = 25.8 bits (54), Expect = 7.0
Identities = 9/33 (27%), Positives = 18/33 (54%)
Frame = +2
Query: 419 GQIVSSITASLRFDGALNVDLTEFQTNLVPTPV 517
G ++ + L+ +G L +D +TN P+P+
Sbjct: 500 GSTITGVNRELQDEGRLEIDAKSSKTNTPPSPL 532
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 25.4 bits (53), Expect = 9.2
Identities = 16/58 (27%), Positives = 29/58 (50%)
Frame = +2
Query: 434 SITASLRFDGALNVDLTEFQTNLVPTPVSTSHWSRTRQSSLPRRPTMNSFPSPRSQTH 607
SIT++ + +VD+ T PVSTS + + + +P P+ S P+ S ++
Sbjct: 221 SITSTESAYTSSSVDIAASTTASSTLPVSTSEATVSFSTDIPATPSTLSSPASSSSSY 278
>SPAC6G9.04 |mug79||meiotically upregulated gene
Mug79|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1318
Score = 25.4 bits (53), Expect = 9.2
Identities = 15/58 (25%), Positives = 27/58 (46%)
Frame = -1
Query: 502 QVSLELGEVHIQSAVESQRSSDRGDNLSDETIQVGVGWALNVEITAADVIDGFIVDHE 329
Q ++ VH ++A E R N + QVG +N ++ D+ + F+ D+E
Sbjct: 601 QSQIKSQSVHHENAAEEIRKMRNLRNSRINSQQVGEKVFVNPDVKTMDIQETFLQDYE 658
>SPAC3F10.07c |mug91||dubious|Schizosaccharomyces pombe|chr
1|||Manual
Length = 172
Score = 25.4 bits (53), Expect = 9.2
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 2/69 (2%)
Frame = +2
Query: 443 ASLRFDGALNVDLTEFQTNLVPTPVSTSHWSRTRQS--SLPRRPTMNSFPSPRSQTHASS 616
ASLR G L++ +TN + + ++ + S +LP T + S +H S
Sbjct: 103 ASLRNMGFLSLVFHTKETNSKSSTLHSNPYCPEHHSIRTLPSAVTATTSNISTSSSHRSD 162
Query: 617 PPTRW*NAT 643
P W N+T
Sbjct: 163 LPNEWTNST 171
>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 595
Score = 25.4 bits (53), Expect = 9.2
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +2
Query: 464 ALNVDLTEFQTNLVPTPVSTSHWSRTRQSSLPRRPTMNSF 583
ALN+D T+F + L S SH + RQ++L N +
Sbjct: 430 ALNIDTTQFPSYLSYHETSVSH-NTYRQNNLEELKNQNDY 468
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,313,393
Number of Sequences: 5004
Number of extensions: 71111
Number of successful extensions: 315
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 311
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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