BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30246 (783 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g17611.1 68416.m02249 rhomboid family protein / zinc finger p... 32 0.37 At4g38200.1 68417.m05392 guanine nucleotide exchange family prot... 29 2.6 At3g29760.1 68416.m03758 NLI interacting factor (NIF) family pro... 29 3.5 At3g13784.1 68416.m01741 beta-fructosidase, putative / beta-fruc... 29 3.5 At3g17611.2 68416.m02250 rhomboid family protein / zinc finger p... 29 4.6 >At3g17611.1 68416.m02249 rhomboid family protein / zinc finger protein-related contains Pfam profiles PF01694: Rhomboid family, PF00641: Zn-finger in Ran binding protein and others Length = 334 Score = 32.3 bits (70), Expect = 0.37 Identities = 25/91 (27%), Positives = 44/91 (48%) Frame = +3 Query: 252 HLIQCTTTPHIFFYLKVVQRTQTQ*VHSIYISKPL*KRGNLPLLYHT*NIQQNTSLGRKD 431 H+ + PH+ F K ++R + ++++P + LL+ I+ TS+G + Sbjct: 59 HISEVWFNPHLIFKHKDLKRLFLSAFY--HVNEPHLVYNMMSLLWK--GIKLETSMGSSE 114 Query: 432 HNAQVEILLNISHGVQLGSVESHLTLFDLTR 524 + V L+ +S GV L +S L LFD R Sbjct: 115 FASMVFTLIGMSQGVTLLLAKSLLLLFDYDR 145 >At4g38200.1 68417.m05392 guanine nucleotide exchange family protein similar to Brefeldin A-inhibited guanine nucleotide-exchange protein 2 [Homo sapiens] SP|Q9Y6D5; contains Pfam profile PF01369: Sec7 domain Length = 1698 Score = 29.5 bits (63), Expect = 2.6 Identities = 17/65 (26%), Positives = 27/65 (41%) Frame = -3 Query: 685 NPQKKNLIRNPQSNLIIKLNKYGTRECGPCTPSAPKRI*AETLFGRQLLVDVHGRVKSNN 506 N +KK ++NP +++ Y + GP P K+ L+D G + NN Sbjct: 944 NLKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNFIANLNLLDQIGSFQLNN 1003 Query: 505 VRCDS 491 V S Sbjct: 1004 VYAHS 1008 >At3g29760.1 68416.m03758 NLI interacting factor (NIF) family protein contains Pfam profile PF03031: NLI interacting factor Length = 465 Score = 29.1 bits (62), Expect = 3.5 Identities = 20/62 (32%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Frame = -1 Query: 408 SVEYFKYGRVVEDFRAFKEVLKYKSNELIVFVFFEPL-LNKKRCVVSWYTG*DEVPYYKK 232 ++E K G V+ + EV K+N+ V E + N++ CV+ T DEVP K Sbjct: 157 TIEKNKEGSVISAVTSNDEVPVVKNNDSCVVSGDETVEKNEEGCVILAVTSSDEVPVVKN 216 Query: 231 NN 226 N+ Sbjct: 217 ND 218 Score = 28.3 bits (60), Expect = 6.1 Identities = 20/62 (32%), Positives = 31/62 (50%), Gaps = 1/62 (1%) Frame = -1 Query: 408 SVEYFKYGRVVEDFRAFKEVLKYKSNELIVFVFFEPL-LNKKRCVVSWYTG*DEVPYYKK 232 +VE + G V+ + EV K+N+ V E + N++ CV+ T DEVP K Sbjct: 87 NVEKKEEGSVISAVTSSDEVPVVKNNDSCVVSGDETVEKNEEGCVILAVTSSDEVPVVKN 146 Query: 231 NN 226 N+ Sbjct: 147 ND 148 >At3g13784.1 68416.m01741 beta-fructosidase, putative / beta-fructofuranosidase, putative / cell wall invertase, putative similar to beta-fructofuranosidase GI:402740 from [Arabidopsis thaliana] Length = 569 Score = 29.1 bits (62), Expect = 3.5 Identities = 13/29 (44%), Positives = 17/29 (58%) Frame = +2 Query: 272 DTTHLFLFKSGSKNTNTMSSFDLYFKTSL 358 + THLF F GS+N N +S K+SL Sbjct: 541 ERTHLFAFNKGSQNVNVLSLSAWSMKSSL 569 >At3g17611.2 68416.m02250 rhomboid family protein / zinc finger protein-related contains Pfam profiles PF01694: Rhomboid family, PF00641: Zn-finger in Ran binding protein and others Length = 239 Score = 28.7 bits (61), Expect = 4.6 Identities = 16/42 (38%), Positives = 23/42 (54%) Frame = +3 Query: 399 IQQNTSLGRKDHNAQVEILLNISHGVQLGSVESHLTLFDLTR 524 I+ TS+G + + V L+ +S GV L +S L LFD R Sbjct: 9 IKLETSMGSSEFASMVFTLIGMSQGVTLLLAKSLLLLFDYDR 50 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,930,447 Number of Sequences: 28952 Number of extensions: 341055 Number of successful extensions: 759 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 750 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 759 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1755792000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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