BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30238 (672 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 131 5e-31 At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 130 6e-31 At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 130 6e-31 At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro... 33 0.13 At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 d... 29 3.7 At3g20840.1 68416.m02635 ovule development protein, putative sim... 29 3.7 At5g53240.1 68418.m06618 expressed protein contains Pfam profile... 28 4.9 At3g03580.1 68416.m00361 pentatricopeptide (PPR) repeat-containi... 28 6.5 At2g26730.1 68415.m03206 leucine-rich repeat transmembrane prote... 28 6.5 At2g38570.1 68415.m04738 expressed protein ; expression supporte... 27 8.6 At1g55590.1 68414.m06363 F-box family protein ; similar to SKP1... 27 8.6 >At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal protein S4, Arabidopsis thaliana, PIR:T48480 Length = 262 Score = 131 bits (316), Expect = 5e-31 Identities = 56/84 (66%), Positives = 71/84 (84%) Frame = +3 Query: 258 VPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTI 437 +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I Sbjct: 136 IPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGVI 195 Query: 438 VSRERHPGSFDIVHIKDSTGHTFA 509 +RE+H GSF+ +HI+DSTGH FA Sbjct: 196 KNREKHKGSFETIHIQDSTGHEFA 219 Score = 103 bits (246), Expect = 1e-22 Identities = 47/65 (72%), Positives = 53/65 (81%) Frame = +1 Query: 61 IKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRV 240 I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + Sbjct: 70 IQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSI 129 Query: 241 ATGPK 255 G K Sbjct: 130 QFGQK 134 Score = 60.9 bits (141), Expect = 8e-10 Identities = 27/45 (60%), Positives = 35/45 (77%) Frame = +2 Query: 494 GTHLRTRLNNVFIIGKGTKAYISLPRGKGIRLTIAEERDKRIAAK 628 G TRL NV+ IGKGTK ++SLP+GKGI+LTI EE KR+A++ Sbjct: 215 GHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTIIEEARKRLASQ 259 >At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) Length = 262 Score = 130 bits (315), Expect = 6e-31 Identities = 56/84 (66%), Positives = 71/84 (84%) Frame = +3 Query: 258 VPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTI 437 +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I Sbjct: 136 IPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVI 195 Query: 438 VSRERHPGSFDIVHIKDSTGHTFA 509 +RE+H GSF+ +HI+DSTGH FA Sbjct: 196 KNREKHKGSFETIHIQDSTGHEFA 219 Score = 103 bits (246), Expect = 1e-22 Identities = 47/65 (72%), Positives = 53/65 (81%) Frame = +1 Query: 61 IKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRV 240 I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + Sbjct: 70 IQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSI 129 Query: 241 ATGPK 255 G K Sbjct: 130 QFGQK 134 Score = 60.9 bits (141), Expect = 8e-10 Identities = 27/45 (60%), Positives = 35/45 (77%) Frame = +2 Query: 494 GTHLRTRLNNVFIIGKGTKAYISLPRGKGIRLTIAEERDKRIAAK 628 G TRL NV+ IGKGTK ++SLP+GKGI+LTI EE KR+A++ Sbjct: 215 GHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTIIEEARKRLASQ 259 >At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains ribosomal protein S4 signature from residues 8 to 22 Length = 261 Score = 130 bits (315), Expect = 6e-31 Identities = 56/84 (66%), Positives = 71/84 (84%) Frame = +3 Query: 258 VPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGTI 437 +PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG I Sbjct: 136 IPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGVI 195 Query: 438 VSRERHPGSFDIVHIKDSTGHTFA 509 +RE+H GSF+ +HI+DSTGH FA Sbjct: 196 KNREKHKGSFETIHIQDSTGHEFA 219 Score = 103 bits (246), Expect = 1e-22 Identities = 47/65 (72%), Positives = 53/65 (81%) Frame = +1 Query: 61 IKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRV 240 I+VDGKVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + Sbjct: 70 IQVDGKVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSI 129 Query: 241 ATGPK 255 G K Sbjct: 130 QFGQK 134 Score = 60.9 bits (141), Expect = 8e-10 Identities = 27/45 (60%), Positives = 35/45 (77%) Frame = +2 Query: 494 GTHLRTRLNNVFIIGKGTKAYISLPRGKGIRLTIAEERDKRIAAK 628 G TRL NV+ IGKGTK ++SLP+GKGI+LTI EE KR++A+ Sbjct: 215 GHEFATRLGNVYTIGKGTKPWVSLPKGKGIKLTIIEEARKRLSAQ 259 >At5g53870.1 68418.m06701 plastocyanin-like domain-containing protein contains similarity to SP|Q02917 Early nodulin 55-2 precursor {Glycine max}; PF02298: Plastocyanin-like domain Length = 370 Score = 33.5 bits (73), Expect = 0.13 Identities = 18/63 (28%), Positives = 23/63 (36%) Frame = +1 Query: 430 APSCPARDIPAPSTLCTSRTPRDTPSHEVEQRVHNRQGHEGVHLAAARQGHPPHHRRGAG 609 A P+ P+P + S +P TPSH + H H A H P H Sbjct: 205 ATPSPSPKSPSPVSHSPSHSPAHTPSHSPAHTPSHSPAHAPSHSPAHAPSHSPAHAPSHS 264 Query: 610 QAH 618 AH Sbjct: 265 PAH 267 >At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 domain-containing protein similar to eukaryotic protein synthesis initiation factor [Homo sapiens] GI:3941724; contains Pfam profiles PF02854: MIF4G domain, PF02847: MA3 domain Length = 1544 Score = 28.7 bits (61), Expect = 3.7 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = +1 Query: 574 QGHPPHHRRGAGQAHRSQG 630 QGHPPH G H++QG Sbjct: 132 QGHPPHPMHHQGMMHQAQG 150 >At3g20840.1 68416.m02635 ovule development protein, putative similar to ovule development protein AINTEGUMENTA (GI:1209099)[Arabidopsis thaliana] Length = 529 Score = 28.7 bits (61), Expect = 3.7 Identities = 12/36 (33%), Positives = 20/36 (55%) Frame = +1 Query: 433 PSCPARDIPAPSTLCTSRTPRDTPSHEVEQRVHNRQ 540 PS + D+ + C S TP ++ HE+++ HN Q Sbjct: 52 PSVQSNDVVVAA--CDSNTPNNSSYHELQESAHNLQ 85 >At5g53240.1 68418.m06618 expressed protein contains Pfam profile PF03478: Protein of unknown function (DUF295) Length = 361 Score = 28.3 bits (60), Expect = 4.9 Identities = 20/77 (25%), Positives = 29/77 (37%) Frame = -2 Query: 575 CRAAARCTPSCPCRL*TRCSTSCEGVSRGVLDVHNVEGAGMSLAGHDGAHTPQVTASRDH 396 CR A + R+ C S + D+ + G+G L G G HTP Sbjct: 156 CRPALKNPEWTNIRIQNPCFFSSRVMFSETHDMFRIPGSGGHLIGSWGLHTPPKIDKLRF 215 Query: 395 TQVPRLKLDEVHNLRSC 345 +P+L + L SC Sbjct: 216 QNLPKLTKTKRKLLHSC 232 >At3g03580.1 68416.m00361 pentatricopeptide (PPR) repeat-containing protein contains INTERPRO:IPR002885 PPR repeats Length = 882 Score = 27.9 bits (59), Expect = 6.5 Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Frame = +2 Query: 482 QGLHGTHLRTRLNNVFIIGKGTKA-YISLPRGKGIRLTIAEERDKR 616 QGLHG L++ +N+V ++ G A Y+ R R + +E D R Sbjct: 227 QGLHGFALKSGVNSVVVVNNGLVAMYLKFRRPTDAR-RVFDEMDVR 271 >At2g26730.1 68415.m03206 leucine-rich repeat transmembrane protein kinase, putative Length = 658 Score = 27.9 bits (59), Expect = 6.5 Identities = 13/44 (29%), Positives = 23/44 (52%) Frame = +3 Query: 276 HDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITG 407 H+ + +P ++N+ I+LDI++ I F NL +TG Sbjct: 124 HNEFSGEFPTSFTQLNNLIRLDISSNNFTGSIPFSVNNLTHLTG 167 >At2g38570.1 68415.m04738 expressed protein ; expression supported by MPSS Length = 302 Score = 27.5 bits (58), Expect = 8.6 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +3 Query: 351 TKIMDFIKFESGNLCMITGGRNLGRVGT 434 T I+ +IK G + GGRN+G+V T Sbjct: 196 TYILVYIKISVGYRVVTPGGRNIGKVST 223 >At1g55590.1 68414.m06363 F-box family protein ; similar to SKP1 interacting partner 2 (SKIP2) TIGR_Ath1:At5g67250 Length = 607 Score = 27.5 bits (58), Expect = 8.6 Identities = 30/112 (26%), Positives = 49/112 (43%), Gaps = 3/112 (2%) Frame = -2 Query: 509 CEGVS-RGVLDVHNVEGA-GMSLAGHDGAHTPQVTASRDHTQVPRLKLDEVHNLRSCDV* 336 C VS RG+ + N EG +L+ D H P ++ HT K ++RSC Sbjct: 448 CRRVSDRGISYLLNNEGTISKTLSTLDLGHMPGISDRAIHTITHCCKALTELSIRSCFHV 507 Query: 335 LDGIVD-FDKWVWVADGAAIMRYQVRNILGPVATRLTLHNLYLASSGVIRWI 183 D ++ W A+G + Q+R +L +HN ++G +RW+ Sbjct: 508 TDSSIESLATWERQAEGGS---KQLR--------KLNVHNCVSLTTGALRWL 548 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,264,118 Number of Sequences: 28952 Number of extensions: 336575 Number of successful extensions: 1068 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 1000 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1062 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1422784080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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