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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30237
         (647 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha...    68   5e-12
At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha...    59   2e-09
At2g47560.1 68415.m05935 zinc finger (C3HC4-type RING finger) fa...    33   0.22 
At3g62690.1 68416.m07042 zinc finger (C3HC4-type RING finger) fa...    31   0.87 
At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containi...    28   4.7  
At5g20600.1 68418.m02446 expressed protein                             27   8.1  
At4g11360.1 68417.m01832 zinc finger (C3HC4-type RING finger) fa...    27   8.1  
At3g57000.1 68416.m06345 nucleolar essential protein-related con...    27   8.1  

>At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha,
            putative contains Pfam PF00400: WD domain, G-beta repeat;
            similar to Coatomer alpha subunit (Alpha-coat protein)
            (Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens]
          Length = 1216

 Score = 68.1 bits (159), Expect = 5e-12
 Identities = 31/63 (49%), Positives = 43/63 (68%)
 Frame = +3

Query: 60   ALNMFFKLKNYRTAASFARRLLELGPRPEVAQQARKILQACEKTPTDEHQLLYDEHNPFS 239
            A+ + +K KN  TA++FARRLLE  P    A+ AR+++QA E+  TDE +L YD  NPF 
Sbjct: 1100 AMGVCYKAKNLATASNFARRLLETSPVDSQAKMARQVVQAAERNMTDETKLNYDFRNPFV 1159

Query: 240  ICG 248
            +CG
Sbjct: 1160 VCG 1162



 Score = 55.2 bits (127), Expect = 4e-08
 Identities = 23/48 (47%), Positives = 29/48 (60%)
 Frame = +2

Query: 260  PIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGLRICPLQ 403
            PIYRG+ +  C  C A F+P  +G +C VC +A IG DA GL   P Q
Sbjct: 1167 PIYRGQKDVSCPYCTARFVPNQEGNICTVCDLAVIGADASGLLCSPSQ 1214


>At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha,
            putative contains Pfam PF00400: WD domain, G-beta repeat;
            similar to Coatomer alpha subunit (Alpha-coat protein)
            (Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens]
          Length = 1218

 Score = 59.3 bits (137), Expect = 2e-09
 Identities = 31/64 (48%), Positives = 40/64 (62%), Gaps = 1/64 (1%)
 Frame = +3

Query: 60   ALNMFFKLKNYRTAASFARRLLELGPRPEV-AQQARKILQACEKTPTDEHQLLYDEHNPF 236
            A+ + +K KN  TAA FAR LL+  P  E  A+ AR+++QA E+  TD   L YD  NPF
Sbjct: 1101 AMTVCYKSKNMATAAHFARSLLDTNPTIESQARTARQVMQAAERNMTDATTLNYDFRNPF 1160

Query: 237  SICG 248
             ICG
Sbjct: 1161 VICG 1164



 Score = 51.2 bits (117), Expect = 6e-07
 Identities = 20/42 (47%), Positives = 27/42 (64%)
 Frame = +2

Query: 260  PIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGL 385
            PIY+G+ +  C  C A F+P  +G +C VC +A IG DA GL
Sbjct: 1169 PIYKGQKDVACPYCTARFVPSQEGNICSVCDLAVIGADASGL 1210



 Score = 27.5 bits (58), Expect = 8.1
 Identities = 10/12 (83%), Positives = 10/12 (83%)
 Frame = +2

Query: 2    AYFTHCKLQPVH 37
            AYFTHCKLQ  H
Sbjct: 1082 AYFTHCKLQTPH 1093


>At2g47560.1 68415.m05935 zinc finger (C3HC4-type RING finger)
           family protein contains Pfam domain, PF00097: Zinc
           finger, C3HC4 type (RING finger)
          Length = 227

 Score = 32.7 bits (71), Expect = 0.22
 Identities = 14/27 (51%), Positives = 18/27 (66%), Gaps = 2/27 (7%)
 Frame = +2

Query: 278 PEEK--CSLCAASFMPEHKGKLCPVCG 352
           PEEK  CS+C + F  E +G+L P CG
Sbjct: 102 PEEKEECSVCLSEFEEEDEGRLLPKCG 128


>At3g62690.1 68416.m07042 zinc finger (C3HC4-type RING finger)
           family protein (ATL5) identical to RING-H2 zinc finger
           protein ATL5 [Arabidopsis thaliana]
           gi|4928401|gb|AAD33583
          Length = 257

 Score = 30.7 bits (66), Expect = 0.87
 Identities = 10/25 (40%), Positives = 17/25 (68%)
 Frame = +2

Query: 278 PEEKCSLCAASFMPEHKGKLCPVCG 352
           P E+CS+C + F  + +G++ P CG
Sbjct: 109 PLEECSVCLSEFEEDDEGRVLPKCG 133


>At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat; gene
           structure supported by cDNA sequence and Brassica genome
           sequence alignments.
          Length = 626

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 14/51 (27%), Positives = 26/51 (50%)
 Frame = +3

Query: 72  FFKLKNYRTAASFARRLLELGPRPEVAQQARKILQACEKTPTDEHQLLYDE 224
           F+K   +  A  F  ++ +L PR     ++ K++  CEK   D+ +  YD+
Sbjct: 435 FYKENRWEDALEFLLKMEKLFPR--AVDRSFKLISLCEKGGMDDLKTAYDQ 483


>At5g20600.1 68418.m02446 expressed protein
          Length = 532

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = +3

Query: 108 FARRLLELGPRPEVAQQARKILQACEK 188
           FA+R  ELG  PE  Q  RK++ A  K
Sbjct: 261 FAKRFYELGSSPECHQGNRKLIFALHK 287


>At4g11360.1 68417.m01832 zinc finger (C3HC4-type RING finger)
           family protein (RHA1b) identical to RING-H2 finger
           protein RHA1b [Arabidopsis thaliana] GI:3790567
          Length = 157

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 9/25 (36%), Positives = 16/25 (64%)
 Frame = +2

Query: 278 PEEKCSLCAASFMPEHKGKLCPVCG 352
           PE+ C++C + F+ + K +  P CG
Sbjct: 81  PEDCCTVCLSDFVSDDKIRQLPKCG 105


>At3g57000.1 68416.m06345 nucleolar essential protein-related
           contains weak similarity to Nucleolar essential protein
           1 (Essential for mitotic growth 1) (Swiss-Prot:Q06287)
           [Saccharomyces cerevisiae]
          Length = 298

 Score = 27.5 bits (58), Expect = 8.1
 Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
 Frame = +3

Query: 93  RTAASFARRLLELGPRPEV-AQQARKILQACEKTPTDEHQLLYDEH 227
           RT   FA  +L+L  +  + A  +R+ L  C K P +EH L  + H
Sbjct: 177 RTFKRFAGIMLQLLQKLSITAVNSREKLLRCVKNPIEEHHLPVNSH 222


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,408,736
Number of Sequences: 28952
Number of extensions: 171809
Number of successful extensions: 533
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 518
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 532
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1344285648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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