BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30237 (647 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha... 68 5e-12 At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha... 59 2e-09 At2g47560.1 68415.m05935 zinc finger (C3HC4-type RING finger) fa... 33 0.22 At3g62690.1 68416.m07042 zinc finger (C3HC4-type RING finger) fa... 31 0.87 At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containi... 28 4.7 At5g20600.1 68418.m02446 expressed protein 27 8.1 At4g11360.1 68417.m01832 zinc finger (C3HC4-type RING finger) fa... 27 8.1 At3g57000.1 68416.m06345 nucleolar essential protein-related con... 27 8.1 >At1g62020.1 68414.m06995 coatomer protein complex, subunit alpha, putative contains Pfam PF00400: WD domain, G-beta repeat; similar to Coatomer alpha subunit (Alpha-coat protein) (Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens] Length = 1216 Score = 68.1 bits (159), Expect = 5e-12 Identities = 31/63 (49%), Positives = 43/63 (68%) Frame = +3 Query: 60 ALNMFFKLKNYRTAASFARRLLELGPRPEVAQQARKILQACEKTPTDEHQLLYDEHNPFS 239 A+ + +K KN TA++FARRLLE P A+ AR+++QA E+ TDE +L YD NPF Sbjct: 1100 AMGVCYKAKNLATASNFARRLLETSPVDSQAKMARQVVQAAERNMTDETKLNYDFRNPFV 1159 Query: 240 ICG 248 +CG Sbjct: 1160 VCG 1162 Score = 55.2 bits (127), Expect = 4e-08 Identities = 23/48 (47%), Positives = 29/48 (60%) Frame = +2 Query: 260 PIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGLRICPLQ 403 PIYRG+ + C C A F+P +G +C VC +A IG DA GL P Q Sbjct: 1167 PIYRGQKDVSCPYCTARFVPNQEGNICTVCDLAVIGADASGLLCSPSQ 1214 >At2g21390.1 68415.m02546 coatomer protein complex, subunit alpha, putative contains Pfam PF00400: WD domain, G-beta repeat; similar to Coatomer alpha subunit (Alpha-coat protein) (Alpha-COP) (HEPCOP) (HEP-COP) (SP:P53621) [Homo sapiens] Length = 1218 Score = 59.3 bits (137), Expect = 2e-09 Identities = 31/64 (48%), Positives = 40/64 (62%), Gaps = 1/64 (1%) Frame = +3 Query: 60 ALNMFFKLKNYRTAASFARRLLELGPRPEV-AQQARKILQACEKTPTDEHQLLYDEHNPF 236 A+ + +K KN TAA FAR LL+ P E A+ AR+++QA E+ TD L YD NPF Sbjct: 1101 AMTVCYKSKNMATAAHFARSLLDTNPTIESQARTARQVMQAAERNMTDATTLNYDFRNPF 1160 Query: 237 SICG 248 ICG Sbjct: 1161 VICG 1164 Score = 51.2 bits (117), Expect = 6e-07 Identities = 20/42 (47%), Positives = 27/42 (64%) Frame = +2 Query: 260 PIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGL 385 PIY+G+ + C C A F+P +G +C VC +A IG DA GL Sbjct: 1169 PIYKGQKDVACPYCTARFVPSQEGNICSVCDLAVIGADASGL 1210 Score = 27.5 bits (58), Expect = 8.1 Identities = 10/12 (83%), Positives = 10/12 (83%) Frame = +2 Query: 2 AYFTHCKLQPVH 37 AYFTHCKLQ H Sbjct: 1082 AYFTHCKLQTPH 1093 >At2g47560.1 68415.m05935 zinc finger (C3HC4-type RING finger) family protein contains Pfam domain, PF00097: Zinc finger, C3HC4 type (RING finger) Length = 227 Score = 32.7 bits (71), Expect = 0.22 Identities = 14/27 (51%), Positives = 18/27 (66%), Gaps = 2/27 (7%) Frame = +2 Query: 278 PEEK--CSLCAASFMPEHKGKLCPVCG 352 PEEK CS+C + F E +G+L P CG Sbjct: 102 PEEKEECSVCLSEFEEEDEGRLLPKCG 128 >At3g62690.1 68416.m07042 zinc finger (C3HC4-type RING finger) family protein (ATL5) identical to RING-H2 zinc finger protein ATL5 [Arabidopsis thaliana] gi|4928401|gb|AAD33583 Length = 257 Score = 30.7 bits (66), Expect = 0.87 Identities = 10/25 (40%), Positives = 17/25 (68%) Frame = +2 Query: 278 PEEKCSLCAASFMPEHKGKLCPVCG 352 P E+CS+C + F + +G++ P CG Sbjct: 109 PLEECSVCLSEFEEDDEGRVLPKCG 133 >At2g17525.1 68415.m02027 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat; gene structure supported by cDNA sequence and Brassica genome sequence alignments. Length = 626 Score = 28.3 bits (60), Expect = 4.7 Identities = 14/51 (27%), Positives = 26/51 (50%) Frame = +3 Query: 72 FFKLKNYRTAASFARRLLELGPRPEVAQQARKILQACEKTPTDEHQLLYDE 224 F+K + A F ++ +L PR ++ K++ CEK D+ + YD+ Sbjct: 435 FYKENRWEDALEFLLKMEKLFPR--AVDRSFKLISLCEKGGMDDLKTAYDQ 483 >At5g20600.1 68418.m02446 expressed protein Length = 532 Score = 27.5 bits (58), Expect = 8.1 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +3 Query: 108 FARRLLELGPRPEVAQQARKILQACEK 188 FA+R ELG PE Q RK++ A K Sbjct: 261 FAKRFYELGSSPECHQGNRKLIFALHK 287 >At4g11360.1 68417.m01832 zinc finger (C3HC4-type RING finger) family protein (RHA1b) identical to RING-H2 finger protein RHA1b [Arabidopsis thaliana] GI:3790567 Length = 157 Score = 27.5 bits (58), Expect = 8.1 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = +2 Query: 278 PEEKCSLCAASFMPEHKGKLCPVCG 352 PE+ C++C + F+ + K + P CG Sbjct: 81 PEDCCTVCLSDFVSDDKIRQLPKCG 105 >At3g57000.1 68416.m06345 nucleolar essential protein-related contains weak similarity to Nucleolar essential protein 1 (Essential for mitotic growth 1) (Swiss-Prot:Q06287) [Saccharomyces cerevisiae] Length = 298 Score = 27.5 bits (58), Expect = 8.1 Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 1/46 (2%) Frame = +3 Query: 93 RTAASFARRLLELGPRPEV-AQQARKILQACEKTPTDEHQLLYDEH 227 RT FA +L+L + + A +R+ L C K P +EH L + H Sbjct: 177 RTFKRFAGIMLQLLQKLSITAVNSREKLLRCVKNPIEEHHLPVNSH 222 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,408,736 Number of Sequences: 28952 Number of extensions: 171809 Number of successful extensions: 533 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 518 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 532 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1344285648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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