BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30230 (708 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 138 9e-34 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 138 9e-34 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 94 2e-20 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 79 8e-16 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 50 2e-07 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 44 3e-05 SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subu... 29 0.65 SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual 29 0.65 SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosa... 29 0.86 SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 27 3.5 SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe... 27 3.5 SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schiz... 26 4.6 SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 4.6 SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccha... 25 8.0 SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|c... 25 8.0 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 138 bits (333), Expect = 9e-34 Identities = 67/94 (71%), Positives = 75/94 (79%) Frame = +3 Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182 PVV+VAVE KN DLPKLVEGLKRL+KSDP V C ESGEHIVAGAGELHLEICLKDL+ Sbjct: 487 PVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQ 546 Query: 183 EDHACIPIKKSDPVVSYRETVAEERTSSVSQSRP 284 EDHA IP+K S PVVSYRE+V+E + + P Sbjct: 547 EDHAGIPLKISPPVVSYRESVSEPSSMTALSKSP 580 Score = 130 bits (315), Expect = 1e-31 Identities = 67/144 (46%), Positives = 92/144 (63%), Gaps = 5/144 (3%) Frame = +2 Query: 269 LSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWC 448 LSKSPNKHNR+FM A+PM + L I+ G VNPRDDFK RAR + +++ +DVT+ARKIWC Sbjct: 576 LSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWC 635 Query: 449 FGPEGTGPNILVDCSKGVQYSMKLR-----TLLWLDSSGPLRKELWLKRFAWC*ISTSMM 613 FGP+ TG N++VD +K V Y +++ W GP+ +E L+ + + + Sbjct: 636 FGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEEN-LRSCRFNILDVVL- 693 Query: 614 *HFHTDAIHRGGGPNHFPTTRRLL 685 H DAIHRGGG PT RR++ Sbjct: 694 ---HADAIHRGGG-QIIPTARRVV 713 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 138 bits (333), Expect = 9e-34 Identities = 67/94 (71%), Positives = 75/94 (79%) Frame = +3 Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182 PVV+VAVE KN DLPKLVEGLKRL+KSDP V C ESGEHIVAGAGELHLEICLKDL+ Sbjct: 487 PVVQVAVEVKNGNDLPKLVEGLKRLSKSDPCVLCTTSESGEHIVAGAGELHLEICLKDLQ 546 Query: 183 EDHACIPIKKSDPVVSYRETVAEERTSSVSQSRP 284 EDHA IP+K S PVVSYRE+V+E + + P Sbjct: 547 EDHAGIPLKISPPVVSYRESVSEPSSMTALSKSP 580 Score = 130 bits (315), Expect = 1e-31 Identities = 67/144 (46%), Positives = 92/144 (63%), Gaps = 5/144 (3%) Frame = +2 Query: 269 LSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWC 448 LSKSPNKHNR+FM A+PM + L I+ G VNPRDDFK RAR + +++ +DVT+ARKIWC Sbjct: 576 LSKSPNKHNRIFMTAEPMSEELSVAIETGHVNPRDDFKVRARIMADEFGWDVTDARKIWC 635 Query: 449 FGPEGTGPNILVDCSKGVQYSMKLR-----TLLWLDSSGPLRKELWLKRFAWC*ISTSMM 613 FGP+ TG N++VD +K V Y +++ W GP+ +E L+ + + + Sbjct: 636 FGPDTTGANVVVDQTKAVAYLNEIKDSVVAAFAWASKEGPMFEEN-LRSCRFNILDVVL- 693 Query: 614 *HFHTDAIHRGGGPNHFPTTRRLL 685 H DAIHRGGG PT RR++ Sbjct: 694 ---HADAIHRGGG-QIIPTARRVV 713 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 93.9 bits (223), Expect = 2e-20 Identities = 45/80 (56%), Positives = 57/80 (71%) Frame = +3 Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182 P+VRVA+EP P ++ KLV GL L ++DP VQ EE+GEH++ AGE+HLE CLKDL Sbjct: 562 PIVRVALEPVRPFEMNKLVTGLDMLNQADPCVQIAVEENGEHVIMCAGEIHLERCLKDLR 621 Query: 183 EDHACIPIKKSDPVVSYRET 242 E A I I+ S P+V YRET Sbjct: 622 ERFAKIEIQASQPLVPYRET 641 Score = 27.5 bits (58), Expect = 2.0 Identities = 17/46 (36%), Positives = 23/46 (50%), Gaps = 4/46 (8%) Frame = +2 Query: 371 DDFKTRARYLTEKYEYDVTEARK----IWCFGPEGTGPNILVDCSK 496 + F R L E+ D+ E + I FGP+ GPNIL D +K Sbjct: 724 EKFYERLSKLLEEENSDLGELKNHLDSIIAFGPKRVGPNILFDKTK 769 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 78.6 bits (185), Expect = 8e-16 Identities = 40/88 (45%), Positives = 59/88 (67%) Frame = +3 Query: 6 VVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEE 185 V +VAVEP NP++LPKL++GL++ KS P+ EESGEH + G GE++++ L DL Sbjct: 600 VFKVAVEPHNPSELPKLLDGLRKTNKSYPLSITKVEESGEHTIFGTGEMYMDCLLYDLRT 659 Query: 186 DHACIPIKKSDPVVSYRETVAEERTSSV 269 ++ I I+ SDPV + ET + TSS+ Sbjct: 660 LYSEIEIRVSDPVARFCETAVD--TSSI 685 Score = 70.5 bits (165), Expect = 2e-13 Identities = 47/148 (31%), Positives = 72/148 (48%), Gaps = 9/148 (6%) Frame = +2 Query: 266 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 445 C S +PNK NR+ M +P+ G+ DI+ G+VN K + + + Y++D+ +R IW Sbjct: 687 CFSDTPNKKNRITMVVEPLEKGISNDIENGKVNINWPQKRISEFFQKNYDWDLLASRSIW 746 Query: 446 CFGPEGTGPNIL------VDCSKGVQYSMK---LRTLLWLDSSGPLRKELWLKRFAWC*I 598 FGP+ G NIL D K V S+K + W GPL E ++ + + Sbjct: 747 AFGPDDRGTNILRDDTLSTDVDKNVLNSVKEYIKQGFQWGTREGPLCDET-IRNVNFRLM 805 Query: 599 STSMM*HFHTDAIHRGGGPNHFPTTRRL 682 + + I+RGGG PT RR+ Sbjct: 806 DVVLA----PEQIYRGGG-QIIPTARRV 828 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 50.4 bits (115), Expect = 2e-07 Identities = 25/85 (29%), Positives = 52/85 (61%), Gaps = 1/85 (1%) Frame = +3 Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDL 179 PV+ ++++PK+ D + L R + DP + ++ ES E I++G GELHLE+ ++ + Sbjct: 474 PVISLSLKPKSK-DTTSFSKALNRFQREDPTFRVQLDNESKETIISGMGELHLEVYVERM 532 Query: 180 EEDHACIPIKKSDPVVSYRETVAEE 254 ++ + + P V++RET++++ Sbjct: 533 RREYK-VDCETGKPRVAFRETLSKK 556 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 43.6 bits (98), Expect = 3e-05 Identities = 26/93 (27%), Positives = 47/93 (50%), Gaps = 1/93 (1%) Frame = +3 Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINE-ESGEHIVAGAGELHLEICLKDL 179 PV ++EP + D P L+E L + + DP + + E+G+ ++ G G +HL++ + L Sbjct: 443 PVCIASIEPYSLKDEPALLEALANMNREDPSFRYTQDLENGQLLIQGMGIMHLQVSYERL 502 Query: 180 EEDHACIPIKKSDPVVSYRETVAEERTSSVSQS 278 + V YRET+ + +SV+ S Sbjct: 503 VSEFGA-RASLGKVQVGYRETLIDVSFNSVTLS 534 >SPAC22H12.02 |tfg3|taf14|transcription factor TFIIF complex subunit Tfg3|Schizosaccharomyces pombe|chr 1|||Manual Length = 241 Score = 29.1 bits (62), Expect = 0.65 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 6/49 (12%) Frame = +3 Query: 36 PADLPKLVEGLKRLAKSD--PMVQCINEESGEHIVA----GAGELHLEI 164 P D+ KL EGL++L + D +VQ +NE + GE H+++ Sbjct: 172 PVDMDKLAEGLQKLQEDDLLQVVQMVNENKTPDMYVRNDIEGGEFHIDL 220 >SPBC947.04 |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Manual Length = 973 Score = 29.1 bits (62), Expect = 0.65 Identities = 19/56 (33%), Positives = 27/56 (48%) Frame = -3 Query: 253 SSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPATMCSPDSSLIHCTMGS 86 S T YDTTG+D ++ SS R WS+ P +P ++ I+ T GS Sbjct: 117 SDYTSVYYDTTGTDDVVYEYHPSSERHKSTNDTWSTNLPT---NPTTTAIYSTSGS 169 >SPBC1289.08 |||UDP-N-acetylglucosamine diphosphorylase |Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 28.7 bits (61), Expect = 0.86 Identities = 19/50 (38%), Positives = 27/50 (54%) Frame = -1 Query: 552 GPLESSHNRVLNFIEY*TPLEQSTRMLGPVPSGPKHQILRASVTSYSYFS 403 G L SSHN +EY +++ + V G KH +LRA+ +Y YFS Sbjct: 288 GLLVSSHNHPC-VVEYSEISDEACKATENV-DGHKHLLLRAANIAYHYFS 335 >SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosaccharomyces pombe|chr 1|||Manual Length = 815 Score = 26.6 bits (56), Expect = 3.5 Identities = 12/46 (26%), Positives = 23/46 (50%) Frame = +2 Query: 344 IDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPNIL 481 ++E + R+ + Y + + VT ++ + FGP GTG +L Sbjct: 502 LEEVKRELRETVQMPVMYAEKFLRFGVTPSKGVLFFGPPGTGKTLL 547 >SPBC32F12.09 |rum1||CDK inhibitor Rum1|Schizosaccharomyces pombe|chr 2|||Manual Length = 230 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -3 Query: 460 LGAKAPNLTGFGNIVFVLFCKIPSAS 383 +G PNL GN +F L ++PS+S Sbjct: 169 IGILPPNLNSIGNKMFSLKSRVPSSS 194 >SPBC36.07 |iki3||RNA polymerase II elongator subunit Iki3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1253 Score = 26.2 bits (55), Expect = 4.6 Identities = 11/18 (61%), Positives = 14/18 (77%) Frame = +2 Query: 332 LPEDIDEGRVNPRDDFKT 385 LPE IDEG+++ DD KT Sbjct: 194 LPEKIDEGKLSDVDDGKT 211 >SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 871 Score = 26.2 bits (55), Expect = 4.6 Identities = 10/22 (45%), Positives = 15/22 (68%) Frame = +2 Query: 269 LSKSPNKHNRLFMKAQPMPDGL 334 ++KS KHN+L K +P+P L Sbjct: 243 INKSVKKHNKLVKKHKPLPSTL 264 >SPAC23E2.03c |ste7||meiotic suppressor protein Ste7|Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.4 bits (53), Expect = 8.0 Identities = 10/33 (30%), Positives = 22/33 (66%), Gaps = 2/33 (6%) Frame = -3 Query: 151 SSPAPATMCSPDSSL--IHCTMGSDLARRLRPS 59 S+ +P+T+CSPD+++ ++ G L ++ P+ Sbjct: 403 SNVSPSTICSPDNNVTFVNLAPGEKLTFKIEPN 435 >SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 240 Score = 25.4 bits (53), Expect = 8.0 Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 3/75 (4%) Frame = +3 Query: 30 KNPADLPKLVEGLKRLAKSDPMVQC-INEES--GEHIVAGAGELHLEICLKDLEEDHACI 200 K P +P L KR KS QC I +E + G G+L+ + + + D + + Sbjct: 16 KEPGKVPPLDIDFKRSVKSSQFSQCAITDEPLYPPIVSCGLGKLYNKASILQMLLDRSSV 75 Query: 201 PIKKSDPVVSYRETV 245 P K + S ++ V Sbjct: 76 P-KSPSHIKSLKDVV 89 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,106,461 Number of Sequences: 5004 Number of extensions: 66656 Number of successful extensions: 233 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 215 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 226 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 329179816 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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