Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30230
(708 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 131 4e-31
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 95 3e-20
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 95 3e-20
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 95 3e-20
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 93 1e-19
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 65 5e-11
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 51 9e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 51 9e-07
At1g70100.3 68414.m08067 expressed protein 29 3.0
At1g70100.2 68414.m08066 expressed protein 29 3.0
At1g70100.1 68414.m08065 expressed protein 29 3.0
At1g67560.1 68414.m07697 lipoxygenase family protein similar to ... 29 3.0
At5g15580.1 68418.m01824 expressed protein unknown protein F14P3... 28 5.3
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 5.3
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 7.0
At5g26210.1 68418.m03119 PHD finger family protein contains Pfam... 28 7.0
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 28 7.0
At3g53160.1 68416.m05858 UDP-glucoronosyl/UDP-glucosyl transfera... 28 7.0
At3g47480.1 68416.m05163 calcium-binding EF hand family protein ... 27 9.2
At1g44120.1 68414.m05096 C2 domain-containing protein / armadill... 27 9.2
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 131 bits (317), Expect = 4e-31
Identities = 68/144 (47%), Positives = 93/144 (64%), Gaps = 5/144 (3%)
Frame = +2
Query: 269 LSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIWC 448
+SKSPNKHNRL+M+A+PM +GL E ID+GR+ PRDD K R++ L E++ +D A+KIW
Sbjct: 577 MSKSPNKHNRLYMEARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWA 636
Query: 449 FGPEGTGPNILVDCSKGVQYSMKLRTLL-----WLDSSGPLRKELWLKRFAWC*ISTSMM 613
FGPE TGPN++VD KGVQY +++ + W GPL +E C ++
Sbjct: 637 FGPETTGPNMVVDMCKGVQYLNEIKDSVVAGFQWASKEGPLAEE---NMRGICFEVCDVV 693
Query: 614 *HFHTDAIHRGGGPNHFPTTRRLL 685
H+DAIHRGGG PT RR++
Sbjct: 694 --LHSDAIHRGGG-QVIPTARRVI 714
Score = 130 bits (315), Expect = 7e-31
Identities = 67/95 (70%), Positives = 74/95 (77%), Gaps = 1/95 (1%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182
PVVRVAV+ K +DLPKLVEGLKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL+
Sbjct: 487 PVVRVAVQCKVASDLPKLVEGLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQ 546
Query: 183 EDH-ACIPIKKSDPVVSYRETVAEERTSSVSQSRP 284
+D I KSDPVVS+RETV + T +V P
Sbjct: 547 DDFMGGAEIIKSDPVVSFRETVCDRSTRTVMSKSP 581
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 95.5 bits (227), Expect = 3e-20
Identities = 44/94 (46%), Positives = 64/94 (68%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182
PVV+ A EP NP++LPK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL
Sbjct: 586 PVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLR 645
Query: 183 EDHACIPIKKSDPVVSYRETVAEERTSSVSQSRP 284
E ++ + +K +DPVVS+ ETV E + P
Sbjct: 646 ELYSEVQVKVADPVVSFCETVVESSSMKCFAETP 679
Score = 68.1 bits (159), Expect = 5e-12
Identities = 31/74 (41%), Positives = 46/74 (62%)
Frame = +2
Query: 266 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 445
C +++PNK N+L M A+P+ GL EDI+ G V+ + + KY++D+ AR IW
Sbjct: 674 CFAETPNKKNKLTMIAEPLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIW 733
Query: 446 CFGPEGTGPNILVD 487
FGP+ G NIL+D
Sbjct: 734 AFGPDKQGTNILLD 747
Score = 37.1 bits (82), Expect = 0.011
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +1
Query: 517 IKDSVVAGFQWAAKEGVMAEEICVVLDFNIYDVTL 621
+KDS+V GFQW A+EG + +E + F I D +
Sbjct: 762 VKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARI 796
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 95.5 bits (227), Expect = 3e-20
Identities = 44/94 (46%), Positives = 64/94 (68%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182
PVV+ A EP NP++LPK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL
Sbjct: 600 PVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLR 659
Query: 183 EDHACIPIKKSDPVVSYRETVAEERTSSVSQSRP 284
E ++ + +K +DPVVS+ ETV E + P
Sbjct: 660 ELYSEVEVKVADPVVSFCETVVESSSMKCFAETP 693
Score = 73.3 bits (172), Expect = 1e-13
Identities = 32/74 (43%), Positives = 48/74 (64%)
Frame = +2
Query: 266 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 445
C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW
Sbjct: 688 CFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIW 747
Query: 446 CFGPEGTGPNILVD 487
FGP+ GPNIL+D
Sbjct: 748 AFGPDKQGPNILLD 761
Score = 37.5 bits (83), Expect = 0.009
Identities = 15/40 (37%), Positives = 25/40 (62%)
Frame = +1
Query: 502 SVLNEIKDSVVAGFQWAAKEGVMAEEICVVLDFNIYDVTL 621
+++ +KDS+V GFQW A+EG + +E + F I D +
Sbjct: 771 NLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARI 810
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 95.5 bits (227), Expect = 3e-20
Identities = 44/94 (46%), Positives = 64/94 (68%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182
PVV+ A EP NP++LPK+VEGL++++KS P+ EESGEH + G GEL+L+ +KDL
Sbjct: 600 PVVKTATEPLNPSELPKMVEGLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLR 659
Query: 183 EDHACIPIKKSDPVVSYRETVAEERTSSVSQSRP 284
E ++ + +K +DPVVS+ ETV E + P
Sbjct: 660 ELYSEVEVKVADPVVSFCETVVESSSMKCFAETP 693
Score = 73.3 bits (172), Expect = 1e-13
Identities = 32/74 (43%), Positives = 48/74 (64%)
Frame = +2
Query: 266 CLSKSPNKHNRLFMKAQPMPDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDVTEARKIW 445
C +++PNK N++ M A+P+ GL EDI+ G V+ + K + KY++D+ AR IW
Sbjct: 688 CFAETPNKKNKITMIAEPLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIW 747
Query: 446 CFGPEGTGPNILVD 487
FGP+ GPNIL+D
Sbjct: 748 AFGPDKQGPNILLD 761
Score = 37.5 bits (83), Expect = 0.009
Identities = 15/40 (37%), Positives = 25/40 (62%)
Frame = +1
Query: 502 SVLNEIKDSVVAGFQWAAKEGVMAEEICVVLDFNIYDVTL 621
+++ +KDS+V GFQW A+EG + +E + F I D +
Sbjct: 771 NLMMAVKDSIVQGFQWGAREGPLCDEPIRNVKFKIVDARI 810
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 93.5 bits (222), Expect = 1e-19
Identities = 42/96 (43%), Positives = 66/96 (68%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLE 182
P +RVA+EP +PAD+ L++GL+ L ++DP V+ GEH++A AGE+HLE C+KDL+
Sbjct: 537 PTLRVAIEPSDPADMSALMKGLRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLK 596
Query: 183 EDHACIPIKKSDPVVSYRETVAEERTSSVSQSRPTS 290
E A + ++ S P+VSYRET+ + ++ + R S
Sbjct: 597 ERFAKVNLEVSPPLVSYRETIEGDGSNLLESLRSLS 632
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 64.9 bits (151), Expect = 5e-11
Identities = 32/84 (38%), Positives = 52/84 (61%), Gaps = 1/84 (1%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQ-CINEESGEHIVAGAGELHLEICLKDL 179
PV++VA+EPK AD+ K+ GL +LA+ DP +EE + ++ G GELHLEI + L
Sbjct: 493 PVIKVAIEPKTKADIDKMATGLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRL 552
Query: 180 EEDHACIPIKKSDPVVSYRETVAE 251
+ + + P V+YRE++++
Sbjct: 553 KREFK-VEANVGAPQVNYRESISK 575
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 50.8 bits (116), Expect = 9e-07
Identities = 26/84 (30%), Positives = 49/84 (58%), Gaps = 1/84 (1%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDL 179
PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I ++ +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 180 EEDHACIPIKKSDPVVSYRETVAE 251
++ + P V++RET+ +
Sbjct: 521 RREYK-VDATVGKPRVNFRETITQ 543
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 50.8 bits (116), Expect = 9e-07
Identities = 26/84 (30%), Positives = 49/84 (58%), Gaps = 1/84 (1%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDL 179
PV+ +AV+P + + + L R K DP + ++ ESG+ I++G GELHL+I ++ +
Sbjct: 461 PVMSLAVQPVSKDSGGQFSKALNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERM 520
Query: 180 EEDHACIPIKKSDPVVSYRETVAE 251
++ + P V++RET+ +
Sbjct: 521 RREYK-VDATVGKPRVNFRETITQ 543
>At1g70100.3 68414.m08067 expressed protein
Length = 504
Score = 29.1 bits (62), Expect = 3.0
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
Frame = +3
Query: 96 VQCINEESGEHIVA-GAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEERTSSVS 272
V C+ EE E + + GE+ E +K+ +++ PIKK+D V A +++ V+
Sbjct: 200 VICMKEEVKEDVPSKDTGEMS-ETLMKETKKEKDHNPIKKTDKNVRTNHMRASPKSNQVT 258
Query: 273 QSRPTSTTVYS*RLSP 320
+ TS V + P
Sbjct: 259 KKPVTSKVVSGRKTQP 274
>At1g70100.2 68414.m08066 expressed protein
Length = 482
Score = 29.1 bits (62), Expect = 3.0
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
Frame = +3
Query: 96 VQCINEESGEHIVA-GAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEERTSSVS 272
V C+ EE E + + GE+ E +K+ +++ PIKK+D V A +++ V+
Sbjct: 200 VICMKEEVKEDVPSKDTGEMS-ETLMKETKKEKDHNPIKKTDKNVRTNHMRASPKSNQVT 258
Query: 273 QSRPTSTTVYS*RLSP 320
+ TS V + P
Sbjct: 259 KKPVTSKVVSGRKTQP 274
>At1g70100.1 68414.m08065 expressed protein
Length = 467
Score = 29.1 bits (62), Expect = 3.0
Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
Frame = +3
Query: 96 VQCINEESGEHIVA-GAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVAEERTSSVS 272
V C+ EE E + + GE+ E +K+ +++ PIKK+D V A +++ V+
Sbjct: 200 VICMKEEVKEDVPSKDTGEMS-ETLMKETKKEKDHNPIKKTDKNVRTNHMRASPKSNQVT 258
Query: 273 QSRPTSTTVYS*RLSP 320
+ TS V + P
Sbjct: 259 KKPVTSKVVSGRKTQP 274
>At1g67560.1 68414.m07697 lipoxygenase family protein similar to
13-lipoxygenase GB:CAA65269 [Solanum tuberosum],
gi:1654140 [Lycopersicon esculentum]
Length = 917
Score = 29.1 bits (62), Expect = 3.0
Identities = 18/56 (32%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Frame = +2
Query: 272 SKSPNKHNRLFMKAQPM-----PDGLPEDIDEGRVNPRDDFKTRARYLTEKYEYDV 424
SK+ N R+ ++QP PDG+ E ++ V+ R D K + Y+YDV
Sbjct: 204 SKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 259
>At5g15580.1 68418.m01824 expressed protein unknown protein F14P3.18
- Arabidopsis thaliana, EMBL:AC009755
Length = 927
Score = 28.3 bits (60), Expect = 5.3
Identities = 14/30 (46%), Positives = 18/30 (60%)
Frame = +1
Query: 358 RESPR*LQDSRSVSYRKVRIRCYRSP*DLV 447
+E+PR DSRS S+R R C P +LV
Sbjct: 223 KETPRLSLDSRSNSFRSARSSCSPEPQELV 252
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 5.3
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3
Query: 105 INEESGEHIVAGAGELHLEICLKDLEEDH 191
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 7.0
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 138 GAGELHLEICLKDLEEDHACIPIKK 212
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At5g26210.1 68418.m03119 PHD finger family protein contains Pfam
domain, PF00628: PHD-finger
Length = 255
Score = 27.9 bits (59), Expect = 7.0
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +2
Query: 257 DQLCLSKSPNKHNRLFMKAQPMPDGLPEDI 346
+ LCL PN+H + + A+ +P LPE +
Sbjct: 46 ENLCLYGHPNEHWEVNLPAEEVPPELPEPV 75
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 27.9 bits (59), Expect = 7.0
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -2
Query: 242 GLTVRHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 126
G TV+ DRV W +++ +++TD +EF N+
Sbjct: 348 GGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At3g53160.1 68416.m05858 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 490
Score = 27.9 bits (59), Expect = 7.0
Identities = 17/46 (36%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = +2
Query: 413 EYDVTEARKIWCFGPEGTGPNILVDCSK-GVQYSM-KLRTLLWLDS 544
EY A K+WC GP + +D +K G + S+ + + L WLDS
Sbjct: 233 EYRKARAGKVWCVGPVSLCNRLGLDKAKRGDKASIGQDQCLQWLDS 278
>At3g47480.1 68416.m05163 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 183
Score = 27.5 bits (58), Expect = 9.2
Identities = 14/45 (31%), Positives = 27/45 (60%), Gaps = 1/45 (2%)
Frame = +3
Query: 3 PVVRVAVEPKNPADLPKLVEGLKRLAK-SDPMVQCINEESGEHIV 134
P+++ V P N AD+ VE +K + S+ + +C+ EE E+++
Sbjct: 63 PIIKKVVVP-NRADIKTSVEEVKAIIDDSEALYECLIEEGEEYLL 106
>At1g44120.1 68414.m05096 C2 domain-containing protein /
armadillo/beta-catenin repeat family protein similar to
CCLS 65 [Silene latifolia] GI:2570102; contains Pfam
profiles PF00514: Armadillo/beta-catenin-like repeat,
PF00168: C2 domain
Length = 2114
Score = 27.5 bits (58), Expect = 9.2
Identities = 21/76 (27%), Positives = 37/76 (48%)
Frame = -3
Query: 259 VRSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPATMCSPDSSLIHCTMGSDL 80
+R S T +T S ++ A + + + S+ SPAP++ +SL++C GS L
Sbjct: 1938 LRQSWTTMPTETARSQAVLAADAIPVLQLMMK-SKLKSPAPSSFHERGNSLLNCLPGS-L 1995
Query: 79 ARRLRPSTSLGRSAGF 32
++ +L RS F
Sbjct: 1996 TVAIKRGDNLKRSNAF 2011
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,784,029
Number of Sequences: 28952
Number of extensions: 372509
Number of successful extensions: 1063
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1026
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1056
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1526202912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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