Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30228
(718 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 79 3e-15
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 79 4e-15
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 72 4e-13
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 70 1e-12
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 36 0.035
At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes... 33 0.19
At3g26445.1 68416.m03297 glycosyl transferase family 17 protein ... 29 2.3
At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,... 29 4.1
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 28 5.4
At1g49760.1 68414.m05580 polyadenylate-binding protein, putative... 28 7.1
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 27 9.4
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 79.0 bits (186), Expect = 3e-15
Identities = 35/48 (72%), Positives = 40/48 (83%)
Frame = +1
Query: 511 TDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSV 654
TDMK++E VR+I+MEGL WGASKLVPVGYGI KL IMC I DD VS+
Sbjct: 156 TDMKKLEEAVRSIQMEGLFWGASKLVPVGYGIKKLHIMCTIVDDLVSI 203
Score = 52.0 bits (119), Expect = 4e-07
Identities = 21/65 (32%), Positives = 39/65 (60%)
Frame = +3
Query: 69 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIAS 248
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + +V RW+N I +
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHIDA 62
Query: 249 YTRLN 263
R++
Sbjct: 63 LLRIS 67
Score = 33.9 bits (74), Expect = 0.11
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2
Query: 425 KAYADKKSKKPALIAKSSILLDVKPWDDK 511
+A + K S K KSS+L+D+KPWDD+
Sbjct: 127 RAASVKASTKKKESGKSSVLMDIKPWDDE 155
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 78.6 bits (185), Expect = 4e-15
Identities = 34/48 (70%), Positives = 41/48 (85%)
Frame = +1
Query: 511 TDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSV 654
TDMK++E V++I+MEGL WGASKLVPVGYGI KLQI+C I DD VS+
Sbjct: 156 TDMKKLEEAVKSIQMEGLFWGASKLVPVGYGIKKLQILCTIVDDLVSI 203
Score = 50.4 bits (115), Expect = 1e-06
Identities = 21/65 (32%), Positives = 38/65 (58%)
Frame = +3
Query: 69 AVGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIAS 248
A ++ + GL L+++L +SY++GY S+ D+ VF + K P + + RWYN I +
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHIDA 62
Query: 249 YTRLN 263
R++
Sbjct: 63 LLRIS 67
Score = 33.9 bits (74), Expect = 0.11
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +2
Query: 425 KAYADKKSKKPALIAKSSILLDVKPWDDK 511
+A + K S K KSS+L+D+KPWDD+
Sbjct: 127 RAASVKASTKKKESGKSSVLIDIKPWDDE 155
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 71.7 bits (168), Expect = 4e-13
Identities = 33/48 (68%), Positives = 38/48 (79%)
Frame = +1
Query: 511 TDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVSV 654
TDMK++E VR+++M GL WGASKLVPVGYGI KL IM I DD VSV
Sbjct: 153 TDMKKLEEAVRSVQMPGLTWGASKLVPVGYGIKKLTIMMTIVDDLVSV 200
Score = 49.6 bits (113), Expect = 2e-06
Identities = 22/58 (37%), Positives = 36/58 (62%)
Frame = +3
Query: 78 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIASY 251
D+ T +GL L ++LA K+Y+SG S DV+V+ V + P P+ +WY+ +AS+
Sbjct: 7 DLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVASH 64
Score = 35.1 bits (77), Expect = 0.047
Identities = 15/26 (57%), Positives = 19/26 (73%)
Frame = +2
Query: 434 ADKKSKKPALIAKSSILLDVKPWDDK 511
A K +KK KSS+LL+VKPWDD+
Sbjct: 127 AKKDTKKTKESGKSSVLLEVKPWDDE 152
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 70.1 bits (164), Expect = 1e-12
Identities = 33/47 (70%), Positives = 36/47 (76%)
Frame = +1
Query: 511 TDMKEMENQVRTIEMEGLLWGASKLVPVGYGINKLQIMCVIEDDKVS 651
TDMK++E VR +EM GL WGASKLVPVGYGI KL IM I DD VS
Sbjct: 149 TDMKKLEEAVRGVEMPGLFWGASKLVPVGYGIKKLTIMFTIVDDLVS 195
Score = 46.4 bits (105), Expect = 2e-05
Identities = 20/57 (35%), Positives = 34/57 (59%)
Frame = +3
Query: 78 DVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRWYNQIAS 248
D+ T +G+ + ++LA K+Y+SG S DV+V+ V P+ P+ +WY +AS
Sbjct: 7 DLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVAS 63
Score = 39.1 bits (87), Expect = 0.003
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +2
Query: 434 ADKKSKKPALIAKSSILLDVKPWDDK 511
A K +KKP KSS+L+DVKPWDD+
Sbjct: 123 AKKDTKKPKESGKSSVLMDVKPWDDE 148
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 35.5 bits (78), Expect = 0.035
Identities = 24/64 (37%), Positives = 32/64 (50%), Gaps = 9/64 (14%)
Frame = +3
Query: 99 LNDLNQYLAEKSYV--SGYTPSQADVQVFEQV-------GKAPAANLPHVLRWYNQIASY 251
L LN LA KS + +G TPS ADV VF + + +PHV+RW N I +
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYIQNK 135
Query: 252 TRLN 263
L+
Sbjct: 136 EELS 139
>At1g04470.1 68414.m00438 expressed protein EST gb|ATTS5672 comes
from this gene
Length = 1035
Score = 33.1 bits (72), Expect = 0.19
Identities = 23/66 (34%), Positives = 28/66 (42%), Gaps = 3/66 (4%)
Frame = +3
Query: 150 TPSQADVQVFEQVGKAPAANLPHVLRWYNQIASYTRLNAKHGLRAPAH---*PPVLNPRL 320
TP A + Q+G+AP N P Y RLN H L + H LNPR+
Sbjct: 743 TPCAASGEELNQMGEAPGGNHPRPSTSRGTQRLYIRLNTLHFLSSQLHSLNKSLSLNPRV 802
Query: 321 PPQQRK 338
P RK
Sbjct: 803 LPATRK 808
>At3g26445.1 68416.m03297 glycosyl transferase family 17 protein
contains Pfam profile PF04724: Glycosyltransferase
family 17
Length = 118
Score = 29.5 bits (63), Expect = 2.3
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = +3
Query: 162 ADVQVFEQVGKAPAANLPHVLRWYNQIASYTRLNAKHGL 278
AD+ + V + P+ + ++LRW N+I L K+ L
Sbjct: 47 ADLLIMSDVDEIPSRHTVNLLRWCNEITQIPHLRLKNSL 85
>At5g20710.1 68418.m02459 beta-galactosidase, putative / lactase,
putative strong similarity to beta-galactosidase
precursor (EC 3.2.1.23) (Lactase) SP:P49676 from
[Brassica oleracea]
Length = 769
Score = 28.7 bits (61), Expect = 4.1
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = -1
Query: 337 FRCWGGSRGFSTGGQWAGALRPCFAFSRVYEAI*LYHRST-WGRLAAGALPTCS 179
F+ WGG + T A ++ F ++ +YH T +GR+A G T S
Sbjct: 202 FKNWGGKHPYRTAEDLAFSVARFFQTGGTFQNYYMYHGGTNFGRVAGGPYITTS 255
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 28.3 bits (60), Expect = 5.4
Identities = 16/32 (50%), Positives = 17/32 (53%)
Frame = +3
Query: 99 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGK 194
L DL Y YVS PSQA V + EQ GK
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGK 525
>At1g49760.1 68414.m05580 polyadenylate-binding protein, putative /
PABP, putative similar to poly(A)-binding protein
GB:AAF66825 GI:7673359 from [Nicotiana tabacum]
Length = 671
Score = 27.9 bits (59), Expect = 7.1
Identities = 21/90 (23%), Positives = 37/90 (41%), Gaps = 1/90 (1%)
Frame = +3
Query: 54 LKETMA-VGDVKTAQGLNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANLPHVLRW 230
L+E A G + + + + D + +V+ TP +A + E GK +V
Sbjct: 343 LREHFAPFGTITSCKVMRDPSGVSRGSGFVAFSTPEEATRAITEMNGKMIVTKPLYVALA 402
Query: 231 YNQIASYTRLNAKHGLRAPAH*PPVLNPRL 320
+ RL A+ P + PP + PR+
Sbjct: 403 QRKEDRKARLQAQFSQMRPVNMPPAVGPRM 432
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 27.5 bits (58), Expect = 9.4
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +3
Query: 99 LNDLNQYLAEKSYVSGYTPSQADVQVFEQVGKAPAANL 212
+N+ + + ++ V G TP+QADV+V VG A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,985,183
Number of Sequences: 28952
Number of extensions: 306616
Number of successful extensions: 839
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 808
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 838
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1555552968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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