Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30225
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g51340.1 68418.m06366 expressed protein 31 0.56
At2g07690.1 68415.m00993 minichromosome maintenance family prote... 31 0.73
At4g01110.1 68417.m00149 hypothetical protein 31 0.97
At1g50770.1 68414.m05710 hypothetical protein 30 1.7
At5g05580.1 68418.m00606 omega-3 fatty acid desaturase, chloropl... 29 3.0
At2g07777.1 68415.m00893 expressed protein 28 5.2
At3g58260.1 68416.m06495 meprin and TRAF homology domain-contain... 28 6.8
At3g47250.3 68416.m05132 expressed protein contains Pfam profile... 28 6.8
At3g47250.2 68416.m05131 expressed protein contains Pfam profile... 28 6.8
At3g47250.1 68416.m05130 expressed protein contains Pfam profile... 28 6.8
At2g46300.1 68415.m05759 expressed protein 28 6.8
At1g27110.1 68414.m03304 hypothetical protein 28 6.8
>At5g51340.1 68418.m06366 expressed protein
Length = 726
Score = 31.5 bits (68), Expect = 0.56
Identities = 12/47 (25%), Positives = 22/47 (46%)
Frame = +3
Query: 240 MFRHRYLHLVSCVLDIAFHLLTVAIVTDGFQCDVNYDKFSAIPWAVV 380
M R +Y H V C + AFH + +T+ + F+A+ + +
Sbjct: 455 MLRGQYSHSVGCYSEAAFHCIEATKLTESISMQASCQAFAAVSYLTI 501
>At2g07690.1 68415.m00993 minichromosome maintenance family protein
/ MCM family protein similar to SP|P55862 DNA
replication licensing factor MCM5 (CDC46 homolog)
{Xenopus laevis}; contains Pfam profile PF00493:
MCM2/3/5 family
Length = 727
Score = 31.1 bits (67), Expect = 0.73
Identities = 29/103 (28%), Positives = 45/103 (43%)
Frame = -3
Query: 320 SDNSNCQQMKRYIQDTRHQMQIAMTKHIVRNLIPQHMLVTCRTIVRTFPIERTARNSPHT 141
+D+ +KRYIQ R + ++K NL Q VT R ++ E T +P
Sbjct: 546 TDSKEDNWLKRYIQYCRARCHPRLSKDAAENL--QRKYVTIRMDMKRRAHE-TGEAAPIP 602
Query: 140 PTIIVLLLIVYTNRSTQKFN*PNYLFS*IFNRKFALFDTTTRE 12
T+ L IV + S K + ++ F LFDT+T +
Sbjct: 603 ITVRQLEAIVRLSESLAKMRLSHEATPDDVDKAFKLFDTSTMD 645
>At4g01110.1 68417.m00149 hypothetical protein
Length = 265
Score = 30.7 bits (66), Expect = 0.97
Identities = 15/63 (23%), Positives = 32/63 (50%)
Frame = +1
Query: 94 VLRFVYTINNKTIIVGVCGELRAVLSIGNVLTMVLHVTSMCCGIKLRTICFVIAICIWCL 273
V R + +K ++V V + + L +G + + VT C G++L+T+ ++ C +
Sbjct: 194 VKRLRADMKSKKLVVKVMAKTKVGLGVGRRKIVTVGVTISCGGVRLQTLDSKMSKCTIKM 253
Query: 274 VSW 282
+ W
Sbjct: 254 LKW 256
>At1g50770.1 68414.m05710 hypothetical protein
Length = 632
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = +3
Query: 213 VLWNQITHYMFRHRYLHLVSCVLDIAFHLLTVAIVTDG 326
VLW +++++F RY H+ +L IA HL + DG
Sbjct: 194 VLW--LSYFVFPSRYYHIYGAILPIAVHLSSDNDEVDG 229
>At5g05580.1 68418.m00606 omega-3 fatty acid desaturase,
chloroplast, temperature-sensitive (FAD8) identical to
SP:48622 Temperature-sensitive omega-3 fatty acid
desaturase, chloroplast precursor (EC 1.14.19.-)
{Arabidopsis thaliana}; contains Pfam profile PF00487:
Fatty acid desaturase; identical to cDNA plastid fatty
acid desaturase GI:1030694
Length = 435
Score = 29.1 bits (62), Expect = 3.0
Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 2/40 (5%)
Frame = +2
Query: 584 LGALYK--RNPEPMRMFLSLSIVKGRDANHFISDTNRIFF 697
LG Y+ +N P+ + L S++K +HF+SDT + +
Sbjct: 384 LGKYYREPKNSGPLPLHLLGSLIKSMKQDHFVSDTGDVVY 423
>At2g07777.1 68415.m00893 expressed protein
Length = 262
Score = 28.3 bits (60), Expect = 5.2
Identities = 13/49 (26%), Positives = 24/49 (48%)
Frame = +2
Query: 122 IKLLLWVCVVNCVQFFQLETFSQWFYTSLACAVESNYALYVSSSLSAFG 268
I L+ +C+ ++ + + +QW Y +L C + L S L+ FG
Sbjct: 38 ILLVFSICLFRSLKQYLGKRMTQWCYLALVCQISLFLVLLRSHILAGFG 86
>At3g58260.1 68416.m06495 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 321
Score = 27.9 bits (59), Expect = 6.8
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = +1
Query: 172 IGNVLTMVLHVTSMCCGIKLRTICFVIAICIWCLVSW 282
I N T V+ S G+++R+ FV+ C W L+++
Sbjct: 5 INNTFTWVIKNLSTLQGLEVRSKIFVVGGCKWRLIAY 41
>At3g47250.3 68416.m05132 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 480
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +3
Query: 276 VLDIAFHLLTVAIVTDGFQCDVNYDKFSAIPW--AVVEPLLLVL 401
+LD F L+ + IV+ D+N D IPW A ++ LL+L
Sbjct: 154 ILDGCFILMLLLIVSRKIDLDMNKDPIFTIPWILASIQSDLLLL 197
>At3g47250.2 68416.m05131 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 480
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +3
Query: 276 VLDIAFHLLTVAIVTDGFQCDVNYDKFSAIPW--AVVEPLLLVL 401
+LD F L+ + IV+ D+N D IPW A ++ LL+L
Sbjct: 154 ILDGCFILMLLLIVSRKIDLDMNKDPIFTIPWILASIQSDLLLL 197
>At3g47250.1 68416.m05130 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function
Length = 480
Score = 27.9 bits (59), Expect = 6.8
Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Frame = +3
Query: 276 VLDIAFHLLTVAIVTDGFQCDVNYDKFSAIPW--AVVEPLLLVL 401
+LD F L+ + IV+ D+N D IPW A ++ LL+L
Sbjct: 154 ILDGCFILMLLLIVSRKIDLDMNKDPIFTIPWILASIQSDLLLL 197
>At2g46300.1 68415.m05759 expressed protein
Length = 252
Score = 27.9 bits (59), Expect = 6.8
Identities = 13/62 (20%), Positives = 26/62 (41%)
Frame = +1
Query: 100 RFVYTINNKTIIVGVCGELRAVLSIGNVLTMVLHVTSMCCGIKLRTICFVIAICIWCLVS 279
R +K +++ V + + L +G + +L V C G+ L + CI +
Sbjct: 188 RLAAKFQSKDLVINVVAKTKVGLGVGGIKIGMLAVNLRCGGVSLNKLDTDSPKCILNTLK 247
Query: 280 WI 285
W+
Sbjct: 248 WV 249
>At1g27110.1 68414.m03304 hypothetical protein
Length = 475
Score = 27.9 bits (59), Expect = 6.8
Identities = 20/68 (29%), Positives = 30/68 (44%), Gaps = 1/68 (1%)
Frame = +3
Query: 219 WNQITHYMFRHRYLHLVSCVLDIAFHLLTVAIVTDGFQCDVNYDKFSAIPWAV-VEPLLL 395
W+ + F H + H+ C L+ H+ V V D Q +K A+ V + L L
Sbjct: 193 WDSCSSLRFSHNWWHVAVCYLEGGSHISKVEEVYD-HQMWKELEKDDAVARDVYTDALGL 251
Query: 396 VLNLGTHG 419
+L L T G
Sbjct: 252 LLRLDTRG 259
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,978,465
Number of Sequences: 28952
Number of extensions: 381586
Number of successful extensions: 977
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 947
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 977
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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