Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30223
(715 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 179 3e-46
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 177 1e-45
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 102 4e-23
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 70 4e-13
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 27 2.0
SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 27 2.0
SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|ch... 27 3.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.1
SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1 |Sch... 26 6.1
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.1
SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr 2|||Ma... 25 8.1
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce... 25 8.1
SPAC2E12.02 |hsf1|hstf, hsf|transcription factor Hsf1|Schizosacc... 25 8.1
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 179 bits (436), Expect = 3e-46
Identities = 80/129 (62%), Positives = 100/129 (77%)
Frame = +3
Query: 228 FQHFLQRDRAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 407
F F GK+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHY
Sbjct: 49 FSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHY 108
Query: 408 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGEVPALGSLPY*WSVSSVDYGKKSKLE 587
T+GKE+VD V D+IR++AD C+GLQGFL+FHSFG G +++Y KKSKL+
Sbjct: 109 TVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQ 168
Query: 588 FAIYPAPQV 614
F++YPAPQV
Sbjct: 169 FSVYPAPQV 177
Score = 93.9 bits (223), Expect = 2e-20
Identities = 41/63 (65%), Positives = 45/63 (71%)
Frame = +1
Query: 85 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGLAS 264
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 265 TYP 273
P
Sbjct: 61 YVP 63
Score = 38.7 bits (86), Expect = 8e-04
Identities = 28/71 (39%), Positives = 37/71 (52%), Gaps = 2/71 (2%)
Frame = +2
Query: 509 GGTGSGFTSLLMERLFR*L--RQEV*TGVRHLPRASGFPLAGRSKPYKLLSFTTPHNPWS 682
GGTGSGF +LL+ERL + ++ V P+ S + +PY S T H
Sbjct: 143 GGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVV----EPYN--SVLTTHATLD 196
Query: 683 TSDCAFMVDNE 715
+DC FMVDNE
Sbjct: 197 LADCTFMVDNE 207
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 177 bits (431), Expect = 1e-45
Identities = 77/129 (59%), Positives = 100/129 (77%)
Frame = +3
Query: 228 FQHFLQRDRAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHY 407
F F GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHY
Sbjct: 53 FGTFFSETGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHY 112
Query: 408 TIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGEVPALGSLPY*WSVSSVDYGKKSKLE 587
T+GKE++D VL+RIR++AD C+GLQGFL+FHSFG G +++YGKKS L+
Sbjct: 113 TVGKEMIDSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQ 172
Query: 588 FAIYPAPQV 614
F++YPAPQV
Sbjct: 173 FSVYPAPQV 181
Score = 87.8 bits (208), Expect = 1e-18
Identities = 45/68 (66%), Positives = 47/68 (69%), Gaps = 5/68 (7%)
Frame = +1
Query: 85 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 249
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 250 TGLASTYP 273
TG P
Sbjct: 60 TGQGKFVP 67
Score = 37.1 bits (82), Expect = 0.002
Identities = 28/71 (39%), Positives = 35/71 (49%), Gaps = 2/71 (2%)
Frame = +2
Query: 509 GGTGSGFTSLLMERLFR*L--RQEV*TGVRHLPRASGFPLAGRSKPYKLLSFTTPHNPWS 682
GGTGSG +LL+ERL + + V P+ S + +PY S T H
Sbjct: 147 GGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVV----EPYN--SVLTTHATLD 200
Query: 683 TSDCAFMVDNE 715
SDC FMVDNE
Sbjct: 201 NSDCTFMVDNE 211
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 102 bits (245), Expect = 4e-23
Identities = 50/118 (42%), Positives = 70/118 (59%)
Frame = +3
Query: 258 GKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLV 437
GK+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D V
Sbjct: 57 GKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAV 116
Query: 438 LDRIRKLADQCTGLQGFLIFHSFGEVPALGSLPY*WSVSSVDYGKKSKLEFAIYPAPQ 611
LD +R+ A+ C LQGF + HS G G S +Y + F++ PAP+
Sbjct: 117 LDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174
Score = 34.7 bits (76), Expect = 0.013
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +1
Query: 85 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDG 186
MRE + + GQ G Q+G A W EHG+ G
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAG 34
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 69.7 bits (163), Expect = 4e-13
Identities = 47/151 (31%), Positives = 75/151 (49%), Gaps = 2/151 (1%)
Frame = +3
Query: 237 FLQRDRAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYT 410
F Q D +++PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+
Sbjct: 52 FYQSDDT-RYIPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YS 109
Query: 411 IGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGEVPALGSLPY*WSVSSVDYGKKSKLEF 590
+ I + ++D I + AD L+GF + HS G + + Y KK +
Sbjct: 110 HAERIFEDIMDMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTY 169
Query: 591 AIYPAPQVFHLPVVRSPTNFYPSPPHTTLGA 683
+++P Q VV P N + TL A
Sbjct: 170 SVFPNSQSVS-DVVVQPYNSLLALKRLTLNA 199
Score = 48.8 bits (111), Expect = 8e-07
Identities = 24/55 (43%), Positives = 33/55 (60%)
Frame = +1
Query: 88 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSET 252
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQS 55
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 27.5 bits (58), Expect = 2.0
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = -3
Query: 320 ADLINNSRFKIDEDS--TGYVLASPVSLKKVLKESSP 216
AD+IN SR+ ID S T + P+SL KE P
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQP 491
>SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 399
Score = 27.5 bits (58), Expect = 2.0
Identities = 14/58 (24%), Positives = 26/58 (44%), Gaps = 2/58 (3%)
Frame = -3
Query: 353 FWMKQLSVCASADLINNSRF--KIDEDSTGYVLASPVSLKKVLKESSPPPMVLSVGIW 186
F + + S +++N+ + KID GY + S K + E + P + G+W
Sbjct: 205 FMAPYIELYGSTYIVSNTNYITKIDYSGRGYFRGTKNSFKATIFEKNEDPDYIVEGVW 262
>SPCC1393.07c |mug4||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 845
Score = 26.6 bits (56), Expect = 3.5
Identities = 11/40 (27%), Positives = 20/40 (50%)
Frame = +3
Query: 558 VDYGKKSKLEFAIYPAPQVFHLPVVRSPTNFYPSPPHTTL 677
V G K+ + ++PAP + L + + P + PHT +
Sbjct: 440 VQLGSKASVPMILFPAPSNYKLTIEKPPFPCDLTIPHTAI 479
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 183 WPDAHRQDHRGWRRFFQHFLQR 248
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC23C4.14 |alg1||mannosyltransferase complex subunit Alg1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 424
Score = 25.8 bits (54), Expect = 6.1
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Frame = -2
Query: 276 HGVRACQPGLAEESVERIVSTPDGLVCGHLAIR-LDAVLQAVKL 148
H VR C P L+ E ++++ D VC H + LD ++ V L
Sbjct: 302 HKVRFCMPWLSIEDYPQVMACADLGVCLHTSSSGLDLPMKVVDL 345
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.1
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 74 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 196
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
>SPBC1105.08 |||EMP70 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 629
Score = 25.4 bits (53), Expect = 8.1
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +1
Query: 532 FLIDGASLPLTTARSLNWSSPSTP 603
+L +GA L L T+ S++W TP
Sbjct: 225 YLPEGADLTLVTSYSVSWKYSDTP 248
>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1067
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/37 (32%), Positives = 22/37 (59%)
Frame = -3
Query: 287 DEDSTGYVLASPVSLKKVLKESSPPPMVLSVGIWPSG 177
++D T Y L P+ + L +++PP +V+ +G P G
Sbjct: 834 EKDLTKY-LGPPIYTSQRLYDTTPPGVVMGLGWTPMG 869
>SPAC2E12.02 |hsf1|hstf, hsf|transcription factor
Hsf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 609
Score = 25.4 bits (53), Expect = 8.1
Identities = 18/61 (29%), Positives = 26/61 (42%)
Frame = -3
Query: 596 DGELQFRLLAVVNGRDAPSIRK*TQSRYLPEGVEDQESLQTGTLVSELADSVQNQIYDFL 417
DGE ++R +V NGR + + P+ Q L E ADS N I ++
Sbjct: 292 DGEGKYR--SVQNGRALNYVSSFNSDSHSPKDYISQSYTNEPGLKKESADSFNNSIDSYI 349
Query: 416 S 414
S
Sbjct: 350 S 350
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,202,674
Number of Sequences: 5004
Number of extensions: 71146
Number of successful extensions: 290
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 206
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 281
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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