Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30221
(596 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g41220.1 68418.m05009 glutathione S-transferase, putative sim... 34 0.063
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 33 0.11
At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / gluta... 33 0.14
At4g29020.1 68417.m04149 glycine-rich protein supporting cDNA gi... 32 0.33
At1g79860.1 68414.m09329 expressed protein contains Pfam profile... 31 0.58
At1g60560.2 68414.m06818 SWIM zinc finger family protein contain... 29 1.8
At1g60560.1 68414.m06817 SWIM zinc finger family protein contain... 29 1.8
At1g77290.1 68414.m09001 tetrachloro-p-hydroquinone reductive de... 29 2.4
At5g41240.1 68418.m05011 glutathione S-transferase, putative sim... 29 3.1
At5g44700.1 68418.m05477 leucine-rich repeat transmembrane prote... 28 4.1
At3g26570.2 68416.m03317 phosphate transporter family protein co... 28 5.4
At3g26570.1 68416.m03316 phosphate transporter family protein co... 28 5.4
At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containi... 28 5.4
At5g38830.1 68418.m04697 tRNA synthetase class I (C) family prot... 27 7.2
At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29) c... 27 7.2
At1g56660.1 68414.m06516 expressed protein 27 7.2
At1g25400.1 68414.m03153 expressed protein similar to unknown pr... 27 7.2
At4g23000.1 68417.m03318 calcineurin-like phosphoesterase family... 27 9.5
At2g30860.1 68415.m03761 glutathione S-transferase, putative ide... 27 9.5
>At5g41220.1 68418.m05009 glutathione S-transferase, putative
similar to emb|CAA10662
Length = 590
Score = 34.3 bits (75), Expect = 0.063
Identities = 26/77 (33%), Positives = 42/77 (54%), Gaps = 9/77 (11%)
Frame = +2
Query: 248 TGTLQLLDKF-LEGQK-YVAGPNL-TVADLSLIASVSSLEASDID-----FKKYANVKRW 403
T +L LD F L+G ++ G N ++ADLSL+ ++ L+ D + NV++W
Sbjct: 141 TKSLTTLDTFWLKGNAMFLLGSNQPSIADLSLVCELTQLQVLDDKDRLRLLSPHKNVEQW 200
Query: 404 YE-TVKSTAPGYQEANE 451
E T K+T P + E +E
Sbjct: 201 IENTRKATMPHFDEVHE 217
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 33.5 bits (73), Expect = 0.11
Identities = 17/52 (32%), Positives = 32/52 (61%)
Frame = +2
Query: 266 LDKFLEGQKYVAGPNLTVADLSLIASVSSLEASDIDFKKYANVKRWYETVKS 421
+++ L G+ Y++G L+V D+ + A+V ++ SD + N +WYE+V S
Sbjct: 17 VEEHLAGKTYISGDQLSVDDVKVYAAVP-VKPSDA----FPNASKWYESVAS 63
>At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative /
glutamyl-tRNA synthetase, putatuve / GluRS, putative
identical to gi:3435196
Length = 719
Score = 33.1 bits (72), Expect = 0.14
Identities = 16/53 (30%), Positives = 30/53 (56%), Gaps = 3/53 (5%)
Frame = +2
Query: 266 LDKFLEGQKYVAGPNLTVADL---SLIASVSSLEASDIDFKKYANVKRWYETV 415
+DK+LE ++ G +L++AD+ S +A S KKY ++ RW+ ++
Sbjct: 104 VDKYLESSTFLVGHSLSIADVAIWSALAGTGQRWESLRKSKKYQSLVRWFNSI 156
>At4g29020.1 68417.m04149 glycine-rich protein supporting cDNA
gi|20465684|gb|AY096677.1|
Length = 158
Score = 31.9 bits (69), Expect = 0.33
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = -2
Query: 475 SLECLQSFLVGLLVTRSCTLNRLVPSLNVGVFLE 374
++ CL FLV L+V+++ T R P+ NVG L+
Sbjct: 2 AVRCLAVFLVALMVSQAVTATRPAPTKNVGAGLD 35
>At1g79860.1 68414.m09329 expressed protein contains Pfam profile
PF03759: Domain of unknown function (DUF315)
Length = 515
Score = 31.1 bits (67), Expect = 0.58
Identities = 19/52 (36%), Positives = 28/52 (53%)
Frame = +2
Query: 341 SVSSLEASDIDFKKYANVKRWYETVKSTAPGYQEANEKGLEAFKGLVNSMLK 496
S S ++ D+D K+ + K W TVK G EA+ + L+ K VN +LK
Sbjct: 220 SYISKDSPDLDGKRN-DEKWWIPTVKVPPDGLSEASRRFLQYQKDCVNQVLK 270
>At1g60560.2 68414.m06818 SWIM zinc finger family protein contains
Pfam domain PF04434: SWIM zinc finger
Length = 500
Score = 29.5 bits (63), Expect = 1.8
Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 5/68 (7%)
Frame = +2
Query: 263 LLDKFLEGQKYVAGPNLTVADLSL-----IASVSSLEASDIDFKKYANVKRWYETVKSTA 427
+L+K +EG + G + TV L+ + + ++D A++K W E K +
Sbjct: 202 VLEKHIEGIQRYCGSDATVDSLASQYVHKLGMIIKRSTHELDLDDQASIKIWAERNKKSI 261
Query: 428 PGYQEANE 451
YQE++E
Sbjct: 262 FFYQESSE 269
>At1g60560.1 68414.m06817 SWIM zinc finger family protein contains
Pfam domain PF04434: SWIM zinc finger
Length = 703
Score = 29.5 bits (63), Expect = 1.8
Identities = 18/68 (26%), Positives = 33/68 (48%), Gaps = 5/68 (7%)
Frame = +2
Query: 263 LLDKFLEGQKYVAGPNLTVADLSL-----IASVSSLEASDIDFKKYANVKRWYETVKSTA 427
+L+K +EG + G + TV L+ + + ++D A++K W E K +
Sbjct: 202 VLEKHIEGIQRYCGSDATVDSLASQYVHKLGMIIKRSTHELDLDDQASIKIWAERNKKSI 261
Query: 428 PGYQEANE 451
YQE++E
Sbjct: 262 FFYQESSE 269
>At1g77290.1 68414.m09001 tetrachloro-p-hydroquinone reductive
dehalogenase-related contains similarity to
tetrachloro-p-hydroquinone reductive dehalogenase
GI:148689 from [Flavobacterium sp.]
Length = 266
Score = 29.1 bits (62), Expect = 2.4
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 3/42 (7%)
Frame = +2
Query: 257 LQLLDKF---LEGQKYVAGPNLTVADLSLIASVSSLEASDID 373
L+LLD+ LEG Y+AG ++AD+ LI ++ L D++
Sbjct: 169 LRLLDEVETKLEGTTYLAGNEFSMADVMLIPVLARLSLLDLE 210
>At5g41240.1 68418.m05011 glutathione S-transferase, putative
similar to glutathione S-transferase, GST 10b
GB:CAA10662 [Arabidopsis thaliana] 37349.
Length = 591
Score = 28.7 bits (61), Expect = 3.1
Identities = 24/77 (31%), Positives = 39/77 (50%), Gaps = 9/77 (11%)
Frame = +2
Query: 248 TGTLQLLDKF-LEGQ-KYV-AGPNLTVADLSLIASVSSLEASDID-----FKKYANVKRW 403
T +L L+ F L+G K++ G ++ADLSL+ + L+ D + V++W
Sbjct: 141 TNSLSTLETFWLKGSAKFLLGGKQPSIADLSLVCELMQLQVLDDKDRLRLLSPHKKVEQW 200
Query: 404 YE-TVKSTAPGYQEANE 451
E T K+T P E +E
Sbjct: 201 IESTRKATMPHSDEVHE 217
>At5g44700.1 68418.m05477 leucine-rich repeat transmembrane protein
kinase, putative
Length = 1252
Score = 28.3 bits (60), Expect = 4.1
Identities = 23/77 (29%), Positives = 36/77 (46%)
Frame = +2
Query: 332 LIASVSSLEASDIDFKKYANVKRWYETVKSTAPGYQEANEKGLEAFKGLVNSMLKK*TLS 511
L+ V+ ++ F + ++ RW ETV T PG EA EK L++S LK L
Sbjct: 1148 LMEIVTGKMPTEAMFDEETDMVRWVETVLDTPPG-SEAREK-------LIDSELKS-LLP 1198
Query: 512 TRNENLFKITSLELNLT 562
E +++ + L T
Sbjct: 1199 CEEEAAYQVLEIALQCT 1215
>At3g26570.2 68416.m03317 phosphate transporter family protein
contains Pfam profile: PF01384 phosphate transporter
family
Length = 587
Score = 27.9 bits (59), Expect = 5.4
Identities = 16/50 (32%), Positives = 28/50 (56%)
Frame = -3
Query: 453 FSLASW*PGAVLLTVSYHLLTLAYFLKSMSEASRLETLAMRLRSATVRFG 304
F ++S A+ + +++ LTL F+KS+ + L+T L AT+ FG
Sbjct: 118 FHISSTTARAISIVIAFSALTLPIFMKSLGQGLALKT--KLLSYATLLFG 165
>At3g26570.1 68416.m03316 phosphate transporter family protein
contains Pfam profile: PF01384 phosphate transporter
family
Length = 613
Score = 27.9 bits (59), Expect = 5.4
Identities = 16/50 (32%), Positives = 28/50 (56%)
Frame = -3
Query: 453 FSLASW*PGAVLLTVSYHLLTLAYFLKSMSEASRLETLAMRLRSATVRFG 304
F ++S A+ + +++ LTL F+KS+ + L+T L AT+ FG
Sbjct: 144 FHISSTTARAISIVIAFSALTLPIFMKSLGQGLALKT--KLLSYATLLFG 191
>At1g78160.1 68414.m09108 pumilio/Puf RNA-binding domain-containing
protein contains Pfam profile: PF00806 Pumilio-family
RNA binding domains (aka PUM-HD, Pumilio homology
domain) (8 copies at C-terminal half of protein)
Length = 650
Score = 27.9 bits (59), Expect = 5.4
Identities = 17/40 (42%), Positives = 23/40 (57%), Gaps = 4/40 (10%)
Frame = -3
Query: 555 FNSSDVILNKFSFRVESVHFLSML----LTSPLNASSPFS 448
FN + N+FSF+ S + LS L SP ++SSPFS
Sbjct: 28 FNGDFSVSNRFSFKSSSDYSLSSSFSNGLCSPEDSSSPFS 67
>At5g38830.1 68418.m04697 tRNA synthetase class I (C) family protein
similar to SP|Q06752 Cysteinyl-tRNA synthetase (EC
6.1.1.16) (Cysteine--tRNA ligase) (CysRS) {Bacillus
subtilis}; contains Pfam profile PF01406: tRNA
synthetases class I (C)
Length = 511
Score = 27.5 bits (58), Expect = 7.2
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -3
Query: 417 LTVSYHLLTLAYFLKSMSEASRLETLAMRLRSAT 316
+T SYH L L +FL S S L A +L S++
Sbjct: 285 ITASYHPLALRHFLMSAQYRSPLSFTASQLESSS 318
>At4g04530.1 68417.m00660 Ulp1 protease family protein (snoR29)
contains Pfam profile PF02902: Ulp1 protease family,
C-terminal catalytic domain; snoR29 gene for small
nucleolar RNA GI:15706258
Length = 917
Score = 27.5 bits (58), Expect = 7.2
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = -2
Query: 355 QAGDTGDETQVGDGEVRSG 299
Q GD GD+T GDG+ + G
Sbjct: 662 QRGDEGDDTHQGDGDPKQG 680
>At1g56660.1 68414.m06516 expressed protein
Length = 522
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/36 (30%), Positives = 20/36 (55%)
Frame = +1
Query: 25 VSSEVPCGSPRKVHHMLKTHRVEPEERGQEQEVHAD 132
+SS K+H +KT ++P+E+G+ EV +
Sbjct: 1 MSSNQENAKEEKLHVKIKTQELDPKEKGENVEVEME 36
>At1g25400.1 68414.m03153 expressed protein similar to unknown
protein GI:6714347 from [Arabidopsis thaliana]
Length = 288
Score = 27.5 bits (58), Expect = 7.2
Identities = 13/29 (44%), Positives = 18/29 (62%)
Frame = -3
Query: 399 LLTLAYFLKSMSEASRLETLAMRLRSATV 313
LL LA+F S +R++TL RLR+ V
Sbjct: 49 LLLLAFFASFTSLTTRIKTLVFRLRNVNV 77
>At4g23000.1 68417.m03318 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 932
Score = 27.1 bits (57), Expect = 9.5
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = -1
Query: 128 ACTSCSCPRSSGSTRCVFNMWWTFRGL 48
AC SCP SS ++++W TF GL
Sbjct: 286 ACNG-SCPDSSDEISPIYSLWATFIGL 311
>At2g30860.1 68415.m03761 glutathione S-transferase, putative
identical to GB:Y12295
Length = 215
Score = 27.1 bits (57), Expect = 9.5
Identities = 19/76 (25%), Positives = 35/76 (46%), Gaps = 3/76 (3%)
Frame = +2
Query: 236 EREGTGTLQLLDKFLEGQKYVAGPNLTVADLSLIASVSSLE---ASDIDFKKYANVKRWY 406
E + G L + + L KY+AG +++ADL+ + L K +V W+
Sbjct: 136 EEKLAGVLDVYEAHLSKSKYLAGDFVSLADLAHLPFTDYLVGPIGKAYMIKDRKHVSAWW 195
Query: 407 ETVKSTAPGYQEANEK 454
+ + S+ P ++E K
Sbjct: 196 DDI-SSRPAWKETVAK 210
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,028,403
Number of Sequences: 28952
Number of extensions: 238746
Number of successful extensions: 687
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 670
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 687
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -