BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30213 (660 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ... 109 3e-25 SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0... 104 1e-23 SPBC215.04 |git11||heterotrimeric G protein gamma subunit Git11 ... 28 1.4 SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces po... 26 4.2 SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyc... 26 5.5 SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit S... 26 5.5 SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 26 5.5 SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomy... 25 7.3 SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2... 25 9.7 SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces pombe... 25 9.7 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 25 9.7 SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces ... 25 9.7 SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosacc... 25 9.7 >SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein S0B|Schizosaccharomyces pombe|chr 1|||Manual Length = 287 Score = 109 bits (263), Expect = 3e-25 Identities = 53/72 (73%), Positives = 57/72 (79%) Frame = +2 Query: 254 PADVFVISSRPFGQRAVLKFAAHTGATPIEGRFTPGAFTNQIQAAFREPRLLIVLDPAQD 433 PADV VISSRP+G RAVLKFAAHTGAT I GRFTPG FTN I +REPRL+IV DP D Sbjct: 74 PADVCVISSRPYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLIIVTDPRAD 133 Query: 434 HQPITEASYVNI 469 Q I EAS+VNI Sbjct: 134 AQAIKEASFVNI 145 Score = 95.9 bits (228), Expect = 5e-21 Identities = 58/163 (35%), Positives = 87/163 (53%), Gaps = 3/163 (1%) Frame = +3 Query: 63 VLALNEEDVTKMLAATTHLGAENVNFQMETYVYKRRADGTHVINLRRTWEKLVLAARAVV 242 VL ++D+ +LAA +H+G++N+ +ME YV+KRR+DG H+INL +TWEKLVLAAR + Sbjct: 10 VLNATDDDIKNLLAADSHIGSKNLEVRMENYVWKRRSDGIHIINLGKTWEKLVLAARVIA 69 Query: 243 AIENPL-MC--SSSHHGPSVSVLY*SLPRTPVLRLLRGVSHQVLLLTRFKLHSVNLVS*L 413 IENP +C SS +G + + + + + +TR L+ + Sbjct: 70 TIENPADVCVISSRPYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITR-TYREPRLI--I 126 Query: 414 YWTLHKTINPLLKLHMSTFPVIALCNTDFPTKFVDIAIPCNTK 542 + + PVIALC+TD VD+AIP N K Sbjct: 127 VTDPRADAQAIKEASFVNIPVIALCDTDSILNHVDVAIPINNK 169 >SPBC685.06 |rps001|rps0-1, rpsa-1, rps0|40S ribosomal protein S0A |Schizosaccharomyces pombe|chr 2|||Manual Length = 292 Score = 104 bits (250), Expect = 1e-23 Identities = 49/72 (68%), Positives = 56/72 (77%) Frame = +2 Query: 254 PADVFVISSRPFGQRAVLKFAAHTGATPIEGRFTPGAFTNQIQAAFREPRLLIVLDPAQD 433 PADV V+S+R +G RAVLKFAAHTGAT I GRFTPG FTN I +REPRL++V DP D Sbjct: 73 PADVCVVSTRTYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITRTYREPRLIVVTDPRAD 132 Query: 434 HQPITEASYVNI 469 Q I EAS+VNI Sbjct: 133 AQAIKEASFVNI 144 Score = 94.7 bits (225), Expect = 1e-20 Identities = 57/167 (34%), Positives = 89/167 (53%), Gaps = 3/167 (1%) Frame = +3 Query: 51 GGLDVLALNEEDVTKMLAATTHLGAENVNFQMETYVYKRRADGTHVINLRRTWEKLVLAA 230 G ++L +ED+ ++LAA H+G++N+ +M+ YV+KRR+DG H++NL +TWEKLVLAA Sbjct: 5 GRPNILNATDEDIKQLLAANCHIGSKNLEVRMDNYVWKRRSDGVHILNLGKTWEKLVLAA 64 Query: 231 RAVVAIENPL-MC--SSSHHGPSVSVLY*SLPRTPVLRLLRGVSHQVLLLTRFKLHSVNL 401 R + IENP +C S+ +G + + + + + +TR L Sbjct: 65 RVIATIENPADVCVVSTRTYGHRAVLKFAAHTGATAIAGRFTPGNFTNYITR-TYREPRL 123 Query: 402 VS*LYWTLHKTINPLLKLHMSTFPVIALCNTDFPTKFVDIAIPCNTK 542 + + + + PVIALC+TD VDIAIP N K Sbjct: 124 I--VVTDPRADAQAIKEASFVNIPVIALCDTDSILNHVDIAIPTNNK 168 >SPBC215.04 |git11||heterotrimeric G protein gamma subunit Git11 |Schizosaccharomyces pombe|chr 2|||Manual Length = 72 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = -1 Query: 660 NSTNNIPKPMVHGGSTPR-KPSNTSRANKPPTIKPNR 553 N NNIP+PM+ + P+ PS +S+ + KP + Sbjct: 22 NYANNIPEPMMVSVAPPKANPSVSSKTKQQQHFKPGK 58 >SPBC354.07c |||oxysterol binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 399 Score = 26.2 bits (55), Expect = 4.2 Identities = 17/55 (30%), Positives = 28/55 (50%), Gaps = 1/55 (1%) Frame = -2 Query: 635 RWF-TGEAHHESLQTLHVPTNHQQLNPIDWEEFGVAWDSNVHKFSGEVCVAQSNH 474 +W+ TG + + + + T + LNPI E F +WDS+ K E+ Q +H Sbjct: 87 KWYITGLSREYASRNKNYGTEKKPLNPILGELFYGSWDSSKGKV--ELTAEQVSH 139 >SPCC970.09 |sec8||exocyst complex subunit Sec8|Schizosaccharomyces pombe|chr 3|||Manual Length = 1088 Score = 25.8 bits (54), Expect = 5.5 Identities = 18/48 (37%), Positives = 22/48 (45%), Gaps = 2/48 (4%) Frame = -2 Query: 269 RTHQRVLDGYDSTSSQ--NKFFPGTTQVDHMGTISTSFVDIGLHLEVN 132 RT + DSTS NK+ G V + S S DIGLH E + Sbjct: 345 RTSAEMFTTVDSTSRAIVNKYSLGNN-VSTVNPFSKSLYDIGLHAETD 391 >SPBC21H7.05 |sfc6||transcription factor TFIIIC complex subunit Sfc6|Schizosaccharomyces pombe|chr 2|||Manual Length = 582 Score = 25.8 bits (54), Expect = 5.5 Identities = 12/33 (36%), Positives = 15/33 (45%) Frame = +3 Query: 456 HMSTFPVIALCNTDFPTKFVDIAIPCNTKFFPI 554 H S + C DFP F+ A C T+ F I Sbjct: 375 HDSYIHNVVQCLDDFPWLFLTTAFDCYTRIFDI 407 >SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.8 bits (54), Expect = 5.5 Identities = 12/45 (26%), Positives = 22/45 (48%) Frame = -2 Query: 266 THQRVLDGYDSTSSQNKFFPGTTQVDHMGTISTSFVDIGLHLEVN 132 T Q ++D D+ + + T V+HMG+ +D G++ N Sbjct: 118 TEQDLIDLADALHDRGMYLMVDTVVNHMGSSDPRNIDYGIYRPFN 162 >SPAC13G7.05 |||acyl-coA-sterol acyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.4 bits (53), Expect = 7.3 Identities = 8/19 (42%), Positives = 11/19 (57%) Frame = -2 Query: 563 NPIDWEEFGVAWDSNVHKF 507 N + W+EF W+ VH F Sbjct: 424 NTVTWDEFSREWNKPVHVF 442 >SPBC1D7.05 |byr2|ste8, SPBC2F12.01|MAP kinase kinase kinase Byr2|Schizosaccharomyces pombe|chr 2|||Manual Length = 659 Score = 25.0 bits (52), Expect = 9.7 Identities = 18/51 (35%), Positives = 22/51 (43%), Gaps = 1/51 (1%) Frame = -2 Query: 194 VDHMGTISTSFVDIGLHLEVNIFCPKMGGCSKHF-GDIFLVEGEHV*SSRH 45 VD+ G I S I LE+N K GG F G F + E V + H Sbjct: 530 VDNKGKIKISDFGISKKLELNSTSTKTGGARPSFQGSSFWMAPEVVKQTMH 580 >SPAC12G12.11c |||DUF544 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 365 Score = 25.0 bits (52), Expect = 9.7 Identities = 11/21 (52%), Positives = 13/21 (61%) Frame = -1 Query: 615 TPRKPSNTSRANKPPTIKPNR 553 TP+K SNT R NK T K + Sbjct: 337 TPKKASNTPRRNKSNTQKSRK 357 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 25.0 bits (52), Expect = 9.7 Identities = 10/23 (43%), Positives = 15/23 (65%) Frame = +3 Query: 489 NTDFPTKFVDIAIPCNTKFFPIY 557 N+ F K +++AIP KF P+Y Sbjct: 280 NSFFTAKALNVAIPGGPKFEPLY 302 >SPAC6B12.02c |mus7||DNA repair protein Mus7|Schizosaccharomyces pombe|chr 1|||Manual Length = 1888 Score = 25.0 bits (52), Expect = 9.7 Identities = 10/28 (35%), Positives = 15/28 (53%) Frame = +1 Query: 154 MSTNDVLMVPM*STCVVPGKNLFWLLVL 237 M+ N V + + T PG N FW L++ Sbjct: 920 MTLNPVFQIGLNGTTHSPGNNSFWPLII 947 >SPAC24C9.14 |otu1|mug141|ubiquitin-specific protease |Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 25.0 bits (52), Expect = 9.7 Identities = 16/49 (32%), Positives = 22/49 (44%), Gaps = 3/49 (6%) Frame = +2 Query: 296 RAVLKFAAHTGATPIEGRFTPGAFTNQIQAAFREPRLLIVLD---PAQD 433 R LK+ + +E T G+F + + A F PR I L P QD Sbjct: 5 RLRLKYENQSAVETVEANATVGSFLDLVAAKFSLPRNSIALKFGFPPQD 53 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,081,963 Number of Sequences: 5004 Number of extensions: 69904 Number of successful extensions: 185 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 176 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 185 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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