Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30208
(713 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 182 3e-47
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 180 2e-46
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 95 1e-20
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 60 3e-10
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 29 0.87
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 26 4.7
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.1
SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces pombe... 26 6.1
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.1
SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 25 8.1
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.1
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 182 bits (444), Expect = 3e-47
Identities = 85/152 (55%), Positives = 112/152 (73%)
Frame = +1
Query: 256 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIR 435
R+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR
Sbjct: 68 RSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIR 127
Query: 436 KLADQCTGLQGFLIFHXXXXXXXXXXLPY*WSVSPLTTGKKSKLEFAIYPAPQVSTAVVE 615
++AD C+GLQGFL+FH + GKKS L+F++YPAPQVST+VVE
Sbjct: 128 RMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVE 187
Query: 616 PLQTLSSPTPTQTLEHSDCAFMVDK*KPSYDI 711
P ++ T TL++SDC FMVD + YDI
Sbjct: 188 PYNSVL--TTHATLDNSDCTFMVDN-EACYDI 216
Score = 95.1 bits (226), Expect = 9e-21
Identities = 48/68 (70%), Positives = 50/68 (73%), Gaps = 5/68 (7%)
Frame = +2
Query: 68 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 232
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 233 TGAGKHVP 256
TG GK VP
Sbjct: 60 TGQGKFVP 67
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 180 bits (438), Expect = 2e-46
Identities = 87/152 (57%), Positives = 110/152 (72%)
Frame = +1
Query: 256 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIR 435
R+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR
Sbjct: 64 RSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIR 123
Query: 436 KLADQCTGLQGFLIFHXXXXXXXXXXLPY*WSVSPLTTGKKSKLEFAIYPAPQVSTAVVE 615
++AD C+GLQGFL+FH + KKSKL+F++YPAPQVST+VVE
Sbjct: 124 RIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVE 183
Query: 616 PLQTLSSPTPTQTLEHSDCAFMVDK*KPSYDI 711
P ++ T TL+ +DC FMVD + YDI
Sbjct: 184 PYNSVL--TTHATLDLADCTFMVDN-ESCYDI 212
Score = 102 bits (244), Expect = 6e-23
Identities = 44/63 (69%), Positives = 49/63 (77%)
Frame = +2
Query: 68 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 247
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 248 HVP 256
+VP
Sbjct: 61 YVP 63
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 94.7 bits (225), Expect = 1e-20
Identities = 54/144 (37%), Positives = 75/144 (52%)
Frame = +1
Query: 256 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIR 435
RAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD +R
Sbjct: 62 RAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVR 121
Query: 436 KLADQCTGLQGFLIFHXXXXXXXXXXLPY*WSVSPLTTGKKSKLEFAIYPAPQVSTAVVE 615
+ A+ C LQGF + H S + F++ PAP+ S VVE
Sbjct: 122 REAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVE 181
Query: 616 PLQTLSSPTPTQTLEHSDCAFMVD 687
P S Q +E+SD F +D
Sbjct: 182 PYNATLS--MHQLVENSDETFCID 203
Score = 44.4 bits (100), Expect = 2e-05
Identities = 22/63 (34%), Positives = 30/63 (47%)
Frame = +2
Query: 68 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 247
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 248 HVP 256
+VP
Sbjct: 59 YVP 61
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 60.1 bits (139), Expect = 3e-10
Identities = 39/128 (30%), Positives = 64/128 (50%), Gaps = 3/128 (2%)
Frame = +1
Query: 256 RAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDR 429
RA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++D
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 430 IRKLADQCTGLQGFLIFHXXXXXXXXXXLPY*WSVSPLTTGKKSKLEFAIYPAPQ-VSTA 606
I + AD L+GF + H + KK ++++P Q VS
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDV 181
Query: 607 VVEPLQTL 630
VV+P +L
Sbjct: 182 VVQPYNSL 189
Score = 52.4 bits (120), Expect = 6e-08
Identities = 25/62 (40%), Positives = 37/62 (59%)
Frame = +2
Query: 71 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 250
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 251 VP 256
+P
Sbjct: 61 IP 62
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 28.7 bits (61), Expect = 0.87
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -3
Query: 303 ADLINNSRFKIDEDSTGTCLPAPVSLKKVLKESSPPPM 190
AD+IN SR+ ID S T + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.2 bits (55), Expect = 4.7
Identities = 13/50 (26%), Positives = 27/50 (54%)
Frame = +1
Query: 529 SVSPLTTGKKSKLEFAIYPAPQVSTAVVEPLQTLSSPTPTQTLEHSDCAF 678
+ +P +TGK + ++ A+Y + + T V+ L LS T + ++ + F
Sbjct: 15 AAAPNSTGKSNGIKKAVYTSTRKKTVGVDDLTLLSKITDEEINKNLELRF 64
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.1
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 147 STASSLMARCPQTRPSGVETILSTLSSARPELAST 251
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPAC25B8.08 |||conserved fungal family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 590
Score = 25.8 bits (54), Expect = 6.1
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +3
Query: 567 GVRHLPRASGFHCRRRAPTNSILTNPHTNP 656
G+ + R+ ++ RR PTN T+P + P
Sbjct: 283 GLEDILRSRSWNLLRRRPTNDASTSPRSTP 312
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.1
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 166 WPDAHRQDHRGWRRFFQHFLQR 231
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 830
Score = 25.4 bits (53), Expect = 8.1
Identities = 12/36 (33%), Positives = 23/36 (63%)
Frame = -3
Query: 525 SIRK*TRAGTSTEGVEDQESLQTGTLVSELADSVQN 418
SIR +R+ +S+ G + Q S+Q+GT ++D ++
Sbjct: 108 SIRSQSRSRSSSVGSDSQASIQSGTNSEVVSDDEED 143
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.1
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 57 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 179
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,999,026
Number of Sequences: 5004
Number of extensions: 62012
Number of successful extensions: 200
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 176
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 333194204
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -