SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30206
         (705 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom...   126   4e-30
SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote...    29   0.49 
SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S...    27   2.0  
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy...    27   2.6  
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe...    26   4.6  
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac...    26   6.0  
SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos...    26   6.0  
SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch...    25   8.0  
SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi...    25   8.0  
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ...    25   8.0  
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi...    25   8.0  

>SPAC926.04c |hsp90|swo1|heat shock protein
           Hsp90|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 704

 Score =  126 bits (303), Expect = 4e-30
 Identities = 60/84 (71%), Positives = 70/84 (83%)
 Frame = +1

Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435
           SL+DP  LD+ K+L+I+I P+K    L+I DTGIGMTK DL+NNLG IAKSGTK FMEA 
Sbjct: 50  SLSDPHALDAEKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAA 109

Query: 436 QAGADISMIGQFGVGFYSSYLVAD 507
            +GADISMIGQFGVGFYS+YLVAD
Sbjct: 110 ASGADISMIGQFGVGFYSAYLVAD 133



 Score = 81.8 bits (193), Expect = 9e-17
 Identities = 39/47 (82%), Positives = 43/47 (91%)
 Frame = +2

Query: 113 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRY 253
           +  ETF F+AEI+QLMSLIINT YSNKEIFLRELISN+SDALDKIRY
Sbjct: 2   SNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRY 48



 Score = 54.4 bits (125), Expect = 2e-08
 Identities = 24/32 (75%), Positives = 26/32 (81%)
 Frame = +3

Query: 510 VTVHSKHNDDEQYVWESSAGGSFTVRPDSGEP 605
           V V SKHNDDEQY+WESSAGGSFTV  D+  P
Sbjct: 135 VQVVSKHNDDEQYIWESSAGGSFTVTLDTDGP 166


>SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein
            Mug36|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1646

 Score = 29.5 bits (63), Expect = 0.49
 Identities = 21/50 (42%), Positives = 23/50 (46%), Gaps = 8/50 (16%)
 Frame = +3

Query: 570  GSFTVRPDSGEP------LGRGYKDSSF--HGQRGTWAEFMGRTPKSKRS 695
            GSFTV  DS EP      L  G KD     H    TW +F GR  +S  S
Sbjct: 1262 GSFTVYADSNEPNAVLNDLDIGLKDLIIYDHLSTSTWNKFFGRDSRSPSS 1311


>SPAC3H1.04c |mdm31||mitochondrial inner membrane protein
           Mdm31|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 601

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 20/53 (37%), Positives = 30/53 (56%)
 Frame = -2

Query: 422 KALVPDFAMVPKLFTKSALVIPIPVSMIVRVPSFLLGMILMYSSLPLSSFDGS 264
           KA++P F      +  +AL+ PI ++ I    +F+   IL + S PLS FDGS
Sbjct: 486 KAVIPIFTNQVS-YINNALIRPI-IAYINSTRTFI--PILCHVSKPLSDFDGS 534


>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 541

 Score = 27.1 bits (57), Expect = 2.6
 Identities = 21/66 (31%), Positives = 35/66 (53%), Gaps = 5/66 (7%)
 Frame = +2

Query: 185 SNKEIFLRELISNSSDALDKIRYDLSRIRQNSI-VAKSCTSRSFPTRTRALLRSSI---- 349
           S+   +L +L+++  D L K+R +  RIR+  I V  S       T TRA+++  +    
Sbjct: 325 SSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRP 384

Query: 350 PVLV*P 367
           PVL+ P
Sbjct: 385 PVLMVP 390


>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 969

 Score = 26.2 bits (55), Expect = 4.6
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = -1

Query: 399 DGSQIVHQIGLGHTNTGIDDRKSALVLVGNDLDVQL 292
           DG   V  I  G     +DDR+  L+ + NDL+V L
Sbjct: 2   DGKPQVEVIVNGQVVPNLDDREYRLIKLENDLEVLL 37


>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 376

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
 Frame = +3

Query: 261 HGSVKTR*WQRAVHQDHSQ-QERGHSYDHRYRYWYDQGRFGEQF 389
           H S K    +R+   D +  +ER H+  +R+R  YD G F + +
Sbjct: 193 HSSDKREHSRRSYRNDRNNWRERTHNDRYRHRDKYDSGYFKKHY 236


>SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate
           synthase|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 584

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 29/131 (22%), Positives = 56/131 (42%), Gaps = 6/131 (4%)
 Frame = +1

Query: 265 DPSKLDSGKELYIKIIPNKNEGTLT-IIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQA 441
           DP  ++S     I++     +G +  ++ T  G+   DL   L     S  K + +    
Sbjct: 385 DPHDVNSEYAAIIRVNSQSGKGGVAYLLKTNCGL---DLPRALQVEFGSIVKDYSDT--K 439

Query: 442 GADISMIGQFGVGFYSSYLVADP*LFTLNTMTTSNT-----CGNLLQEARSQSAQTAVSP 606
           G ++S IG+    FY++Y +  P  F++N  T S+      C   + + + +   T    
Sbjct: 440 GKELS-IGEISDLFYTTYYLEFPGRFSVNDYTLSSNGPQSKCIKCVVDIKGEKKDTPSRV 498

Query: 607 LVEGTKIRPFT 639
           ++EG    P +
Sbjct: 499 VIEGVGNGPLS 509


>SPAC4F10.18 |||WD repeat protein, human NUP37
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 391

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 11/19 (57%), Positives = 13/19 (68%)
 Frame = +1

Query: 496 LVADP*LFTLNTMTTSNTC 552
           LV +P L TLNT+   NTC
Sbjct: 228 LVKNPWLLTLNTLPLVNTC 246


>SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 817

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 8/29 (27%), Positives = 16/29 (55%)
 Frame = +3

Query: 303 QDHSQQERGHSYDHRYRYWYDQGRFGEQF 389
           +DH++++  H+     +Y YD  R  E +
Sbjct: 603 KDHTKEQAWHAMQQGIKYMYDNWRIWENY 631


>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1044

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 14/64 (21%), Positives = 32/64 (50%)
 Frame = +2

Query: 86  MPEEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYDLSR 265
           + E++  + A+ E+F      A+L    +N    NKE  + +L +  S+   +++   S+
Sbjct: 762 LTEKLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQ 821

Query: 266 IRQN 277
           + Q+
Sbjct: 822 LNQD 825


>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
            homolog|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 3071

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 12/43 (27%), Positives = 22/43 (51%)
 Frame = +1

Query: 265  DPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLG 393
            DPSK     ++Y K+   + +  L   D+GI ++   L+  +G
Sbjct: 2472 DPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIG 2514


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,071,609
Number of Sequences: 5004
Number of extensions: 67552
Number of successful extensions: 202
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 201
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -