BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30206 (705 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 126 4e-30 SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated prote... 29 0.49 SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S... 27 2.0 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 27 2.6 SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 26 4.6 SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 26 6.0 SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizos... 26 6.0 SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosacch... 25 8.0 SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 25 8.0 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 8.0 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 8.0 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 126 bits (303), Expect = 4e-30 Identities = 60/84 (71%), Positives = 70/84 (83%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 SL+DP LD+ K+L+I+I P+K L+I DTGIGMTK DL+NNLG IAKSGTK FMEA Sbjct: 50 SLSDPHALDAEKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAA 109 Query: 436 QAGADISMIGQFGVGFYSSYLVAD 507 +GADISMIGQFGVGFYS+YLVAD Sbjct: 110 ASGADISMIGQFGVGFYSAYLVAD 133 Score = 81.8 bits (193), Expect = 9e-17 Identities = 39/47 (82%), Positives = 43/47 (91%) Frame = +2 Query: 113 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRY 253 + ETF F+AEI+QLMSLIINT YSNKEIFLRELISN+SDALDKIRY Sbjct: 2 SNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRY 48 Score = 54.4 bits (125), Expect = 2e-08 Identities = 24/32 (75%), Positives = 26/32 (81%) Frame = +3 Query: 510 VTVHSKHNDDEQYVWESSAGGSFTVRPDSGEP 605 V V SKHNDDEQY+WESSAGGSFTV D+ P Sbjct: 135 VQVVSKHNDDEQYIWESSAGGSFTVTLDTDGP 166 >SPBC31E1.01c |atg2|mug36, SPBC660.18c|autophagy associated protein Mug36|Schizosaccharomyces pombe|chr 2|||Manual Length = 1646 Score = 29.5 bits (63), Expect = 0.49 Identities = 21/50 (42%), Positives = 23/50 (46%), Gaps = 8/50 (16%) Frame = +3 Query: 570 GSFTVRPDSGEP------LGRGYKDSSF--HGQRGTWAEFMGRTPKSKRS 695 GSFTV DS EP L G KD H TW +F GR +S S Sbjct: 1262 GSFTVYADSNEPNAVLNDLDIGLKDLIIYDHLSTSTWNKFFGRDSRSPSS 1311 >SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 27.5 bits (58), Expect = 2.0 Identities = 20/53 (37%), Positives = 30/53 (56%) Frame = -2 Query: 422 KALVPDFAMVPKLFTKSALVIPIPVSMIVRVPSFLLGMILMYSSLPLSSFDGS 264 KA++P F + +AL+ PI ++ I +F+ IL + S PLS FDGS Sbjct: 486 KAVIPIFTNQVS-YINNALIRPI-IAYINSTRTFI--PILCHVSKPLSDFDGS 534 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 27.1 bits (57), Expect = 2.6 Identities = 21/66 (31%), Positives = 35/66 (53%), Gaps = 5/66 (7%) Frame = +2 Query: 185 SNKEIFLRELISNSSDALDKIRYDLSRIRQNSI-VAKSCTSRSFPTRTRALLRSSI---- 349 S+ +L +L+++ D L K+R + RIR+ I V S T TRA+++ + Sbjct: 325 SSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRP 384 Query: 350 PVLV*P 367 PVL+ P Sbjct: 385 PVLMVP 390 >SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 26.2 bits (55), Expect = 4.6 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = -1 Query: 399 DGSQIVHQIGLGHTNTGIDDRKSALVLVGNDLDVQL 292 DG V I G +DDR+ L+ + NDL+V L Sbjct: 2 DGKPQVEVIVNGQVVPNLDDREYRLIKLENDLEVLL 37 >SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 25.8 bits (54), Expect = 6.0 Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 1/44 (2%) Frame = +3 Query: 261 HGSVKTR*WQRAVHQDHSQ-QERGHSYDHRYRYWYDQGRFGEQF 389 H S K +R+ D + +ER H+ +R+R YD G F + + Sbjct: 193 HSSDKREHSRRSYRNDRNNWRERTHNDRYRHRDKYDSGYFKKHY 236 >SPBC3E7.16c |leu3|SPBC4F6.03c|2-isopropylmalate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 25.8 bits (54), Expect = 6.0 Identities = 29/131 (22%), Positives = 56/131 (42%), Gaps = 6/131 (4%) Frame = +1 Query: 265 DPSKLDSGKELYIKIIPNKNEGTLT-IIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQA 441 DP ++S I++ +G + ++ T G+ DL L S K + + Sbjct: 385 DPHDVNSEYAAIIRVNSQSGKGGVAYLLKTNCGL---DLPRALQVEFGSIVKDYSDT--K 439 Query: 442 GADISMIGQFGVGFYSSYLVADP*LFTLNTMTTSNT-----CGNLLQEARSQSAQTAVSP 606 G ++S IG+ FY++Y + P F++N T S+ C + + + + T Sbjct: 440 GKELS-IGEISDLFYTTYYLEFPGRFSVNDYTLSSNGPQSKCIKCVVDIKGEKKDTPSRV 498 Query: 607 LVEGTKIRPFT 639 ++EG P + Sbjct: 499 VIEGVGNGPLS 509 >SPAC4F10.18 |||WD repeat protein, human NUP37 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 391 Score = 25.4 bits (53), Expect = 8.0 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = +1 Query: 496 LVADP*LFTLNTMTTSNTC 552 LV +P L TLNT+ NTC Sbjct: 228 LVKNPWLLTLNTLPLVNTC 246 >SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 25.4 bits (53), Expect = 8.0 Identities = 8/29 (27%), Positives = 16/29 (55%) Frame = +3 Query: 303 QDHSQQERGHSYDHRYRYWYDQGRFGEQF 389 +DH++++ H+ +Y YD R E + Sbjct: 603 KDHTKEQAWHAMQQGIKYMYDNWRIWENY 631 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 25.4 bits (53), Expect = 8.0 Identities = 14/64 (21%), Positives = 32/64 (50%) Frame = +2 Query: 86 MPEEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYDLSR 265 + E++ + A+ E+F A+L +N NKE + +L + S+ +++ S+ Sbjct: 762 LTEKLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQ 821 Query: 266 IRQN 277 + Q+ Sbjct: 822 LNQD 825 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 25.4 bits (53), Expect = 8.0 Identities = 12/43 (27%), Positives = 22/43 (51%) Frame = +1 Query: 265 DPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLG 393 DPSK ++Y K+ + + L D+GI ++ L+ +G Sbjct: 2472 DPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIG 2514 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,071,609 Number of Sequences: 5004 Number of extensions: 67552 Number of successful extensions: 202 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 194 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 201 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 327172622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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