BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30206 (705 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / hea... 140 6e-34 At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearl... 140 8e-34 At5g56010.1 68418.m06989 heat shock protein, putative strong sim... 140 8e-34 At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearl... 140 8e-34 At2g04030.2 68415.m00372 heat shock protein, putative strong sim... 101 6e-22 At2g04030.1 68415.m00371 heat shock protein, putative strong sim... 101 6e-22 At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata format... 100 2e-21 At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata format... 100 2e-21 At3g07770.1 68416.m00947 heat shock protein-related strong simil... 91 5e-19 At3g51350.1 68416.m05622 aspartyl protease family protein contai... 31 0.56 At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-contain... 29 3.0 At5g63470.1 68418.m07968 CCAAT-box binding transcription factor ... 28 6.9 At1g26170.1 68414.m03194 importin beta-2 subunit family protein ... 28 6.9 At4g32140.1 68417.m04572 expressed protein 27 9.2 At3g25570.1 68416.m03180 adenosylmethionine decarboxylase family... 27 9.2 At2g04620.1 68415.m00470 cation efflux family protein potential ... 27 9.2 >At5g52640.1 68418.m06535 heat shock protein 81-1 (HSP81-1) / heat shock protein 83 (HSP83) nearly identical to SP|P27323 Heat shock protein 81-1 (HSP81-1) (Heat shock protein 83) {Arabidopsis thaliana}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 705 Score = 140 bits (340), Expect = 6e-34 Identities = 77/132 (58%), Positives = 95/132 (71%), Gaps = 5/132 (3%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 SLTD SKLD EL+I+++P+K+ TL+IID+GIGMTKADLVNNLGTIA+SGTK FMEAL Sbjct: 55 SLTDKSKLDGQPELFIRLVPDKSNKTLSIIDSGIGMTKADLVNNLGTIARSGTKEFMEAL 114 Query: 436 QAGADISMIGQFGVGFYSSYLVADP*LFTLNTMTTSNTCGNLLQEARSQSAQTAV----- 600 QAGAD+SMIGQFGVGFYS+YLVA+ + T T N + E+++ + T Sbjct: 115 QAGADVSMIGQFGVGFYSAYLVAEKVVVT----TKHNDDEQYVWESQAGGSFTVTRDVDG 170 Query: 601 SPLVEGTKIRPF 636 PL GTKI F Sbjct: 171 EPLGRGTKITLF 182 Score = 89.8 bits (213), Expect = 2e-18 Identities = 43/52 (82%), Positives = 48/52 (92%) Frame = +2 Query: 101 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYD 256 + Q A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR++ Sbjct: 3 DVQMADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFE 54 >At5g56030.1 68418.m06991 heat shock protein 81-2 (HSP81-2) nearly identical to SP|P55737 Heat shock protein 81-2 (HSP81-2) {Arabidopsis thaliana} Length = 699 Score = 140 bits (339), Expect = 8e-34 Identities = 68/84 (80%), Positives = 74/84 (88%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 SLTD SKLD EL+I IIP+K TLTIID+GIGMTKADLVNNLGTIA+SGTK FMEAL Sbjct: 50 SLTDKSKLDGQPELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEAL 109 Query: 436 QAGADISMIGQFGVGFYSSYLVAD 507 AGAD+SMIGQFGVGFYS+YLVAD Sbjct: 110 AAGADVSMIGQFGVGFYSAYLVAD 133 Score = 87.8 bits (208), Expect = 6e-18 Identities = 42/48 (87%), Positives = 46/48 (95%) Frame = +2 Query: 113 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYD 256 A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR++ Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFE 49 Score = 62.1 bits (144), Expect = 3e-10 Identities = 30/39 (76%), Positives = 31/39 (79%), Gaps = 1/39 (2%) Frame = +3 Query: 510 VTVHSKHNDDEQYVWESSAGGSFTV-RPDSGEPLGRGYK 623 V V +KHNDDEQYVWES AGGSFTV R SGE LGRG K Sbjct: 135 VVVTTKHNDDEQYVWESQAGGSFTVTRDTSGETLGRGTK 173 >At5g56010.1 68418.m06989 heat shock protein, putative strong similarity to SP|P55737 Heat shock protein 81-2 (HSP81-2) {Arabidopsis thaliana}; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 699 Score = 140 bits (339), Expect = 8e-34 Identities = 68/84 (80%), Positives = 74/84 (88%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 SLTD SKLD EL+I IIP+K TLTIID+GIGMTKADLVNNLGTIA+SGTK FMEAL Sbjct: 50 SLTDKSKLDGQPELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEAL 109 Query: 436 QAGADISMIGQFGVGFYSSYLVAD 507 AGAD+SMIGQFGVGFYS+YLVAD Sbjct: 110 AAGADVSMIGQFGVGFYSAYLVAD 133 Score = 87.8 bits (208), Expect = 6e-18 Identities = 42/48 (87%), Positives = 46/48 (95%) Frame = +2 Query: 113 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYD 256 A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR++ Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFE 49 Score = 62.1 bits (144), Expect = 3e-10 Identities = 30/39 (76%), Positives = 31/39 (79%), Gaps = 1/39 (2%) Frame = +3 Query: 510 VTVHSKHNDDEQYVWESSAGGSFTV-RPDSGEPLGRGYK 623 V V +KHNDDEQYVWES AGGSFTV R SGE LGRG K Sbjct: 135 VVVTTKHNDDEQYVWESQAGGSFTVTRDTSGEALGRGTK 173 >At5g56000.1 68418.m06988 heat shock protein 81-4 (HSP81-4) nearly identical to heat shock protein hsp81.4 [Arabidopsis thaliana] GI:1906828; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 699 Score = 140 bits (339), Expect = 8e-34 Identities = 68/84 (80%), Positives = 74/84 (88%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 SLTD SKLD EL+I IIP+K TLTIID+GIGMTKADLVNNLGTIA+SGTK FMEAL Sbjct: 50 SLTDKSKLDGQPELFIHIIPDKTNNTLTIIDSGIGMTKADLVNNLGTIARSGTKEFMEAL 109 Query: 436 QAGADISMIGQFGVGFYSSYLVAD 507 AGAD+SMIGQFGVGFYS+YLVAD Sbjct: 110 AAGADVSMIGQFGVGFYSAYLVAD 133 Score = 87.8 bits (208), Expect = 6e-18 Identities = 42/48 (87%), Positives = 46/48 (95%) Frame = +2 Query: 113 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYD 256 A+ ETFAFQAEI QL+SLIINTFYSNKEIFLRELISNSSDALDKIR++ Sbjct: 2 ADAETFAFQAEINQLLSLIINTFYSNKEIFLRELISNSSDALDKIRFE 49 Score = 62.1 bits (144), Expect = 3e-10 Identities = 30/39 (76%), Positives = 31/39 (79%), Gaps = 1/39 (2%) Frame = +3 Query: 510 VTVHSKHNDDEQYVWESSAGGSFTV-RPDSGEPLGRGYK 623 V V +KHNDDEQYVWES AGGSFTV R SGE LGRG K Sbjct: 135 VVVTTKHNDDEQYVWESQAGGSFTVTRDTSGEALGRGTK 173 >At2g04030.2 68415.m00372 heat shock protein, putative strong similarity to heat shock protein [Arabidopsis thaliana] GI:1906830; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 777 Score = 101 bits (241), Expect = 6e-22 Identities = 50/88 (56%), Positives = 68/88 (77%), Gaps = 4/88 (4%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 S+T+PS L G +L I+I P+ + GT+TI DTGIGMTK +L++ LGTIA+SGT F++AL Sbjct: 122 SVTEPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKFLKAL 181 Query: 436 Q----AGADISMIGQFGVGFYSSYLVAD 507 + GAD +IGQFGVGFYS++LVA+ Sbjct: 182 KENKDLGADNGLIGQFGVGFYSAFLVAE 209 Score = 67.7 bits (158), Expect = 7e-12 Identities = 28/54 (51%), Positives = 44/54 (81%) Frame = +2 Query: 92 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRY 253 E+ T+ E F +QAE+++L+ LI+++ YS+KE+FLREL+SN+SDALDK+R+ Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDKLRF 120 >At2g04030.1 68415.m00371 heat shock protein, putative strong similarity to heat shock protein [Arabidopsis thaliana] GI:1906830; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 780 Score = 101 bits (241), Expect = 6e-22 Identities = 50/88 (56%), Positives = 68/88 (77%), Gaps = 4/88 (4%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 S+T+PS L G +L I+I P+ + GT+TI DTGIGMTK +L++ LGTIA+SGT F++AL Sbjct: 122 SVTEPSLLGDGGDLEIRIKPDPDNGTITITDTGIGMTKEELIDCLGTIAQSGTSKFLKAL 181 Query: 436 Q----AGADISMIGQFGVGFYSSYLVAD 507 + GAD +IGQFGVGFYS++LVA+ Sbjct: 182 KENKDLGADNGLIGQFGVGFYSAFLVAE 209 Score = 67.7 bits (158), Expect = 7e-12 Identities = 28/54 (51%), Positives = 44/54 (81%) Frame = +2 Query: 92 EEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRY 253 E+ T+ E F +QAE+++L+ LI+++ YS+KE+FLREL+SN+SDALDK+R+ Sbjct: 67 EKETTEEGSGEKFEYQAEVSRLLDLIVHSLYSHKEVFLRELVSNASDALDKLRF 120 >At4g24190.2 68417.m03473 shepherd protein (SHD) / clavata formation protein, putative nearly identical to SHEPHERD [Arabidopsis thaliana] GI:19570872; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 823 Score = 99.5 bits (237), Expect = 2e-21 Identities = 60/132 (45%), Positives = 82/132 (62%), Gaps = 7/132 (5%) Frame = +1 Query: 256 SLTDPSKLDSGK--ELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFME 429 +LTD L G +L I+I +K + L+I D GIGMTK DL+ NLGTIAKSGT AF+E Sbjct: 122 ALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVE 181 Query: 430 ALQAGADISMIGQFGVGFYSSYLVADP*LFTLNTMTTSNTCGNLLQEARSQ-----SAQT 594 +Q+ D+++IGQFGVGFYS+YLVAD + ++ N + E+++ S T Sbjct: 182 KMQSSGDLNLIGQFGVGFYSAYLVAD----YIEVISKHNDDSQYVWESKANGKFAVSEDT 237 Query: 595 AVSPLVEGTKIR 630 PL GT+IR Sbjct: 238 WNEPLGRGTEIR 249 Score = 72.9 bits (171), Expect = 2e-13 Identities = 33/51 (64%), Positives = 43/51 (84%) Frame = +2 Query: 101 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRY 253 +T + E F FQAE+++LM +IIN+ YSNK+IFLRELISN+SDALDKIR+ Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRF 120 >At4g24190.1 68417.m03472 shepherd protein (SHD) / clavata formation protein, putative nearly identical to SHEPHERD [Arabidopsis thaliana] GI:19570872; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 823 Score = 99.5 bits (237), Expect = 2e-21 Identities = 60/132 (45%), Positives = 82/132 (62%), Gaps = 7/132 (5%) Frame = +1 Query: 256 SLTDPSKLDSGK--ELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFME 429 +LTD L G +L I+I +K + L+I D GIGMTK DL+ NLGTIAKSGT AF+E Sbjct: 122 ALTDKDVLGEGDTAKLEIQIKLDKAKKILSIRDRGIGMTKEDLIKNLGTIAKSGTSAFVE 181 Query: 430 ALQAGADISMIGQFGVGFYSSYLVADP*LFTLNTMTTSNTCGNLLQEARSQ-----SAQT 594 +Q+ D+++IGQFGVGFYS+YLVAD + ++ N + E+++ S T Sbjct: 182 KMQSSGDLNLIGQFGVGFYSAYLVAD----YIEVISKHNDDSQYVWESKANGKFAVSEDT 237 Query: 595 AVSPLVEGTKIR 630 PL GT+IR Sbjct: 238 WNEPLGRGTEIR 249 Score = 72.9 bits (171), Expect = 2e-13 Identities = 33/51 (64%), Positives = 43/51 (84%) Frame = +2 Query: 101 ETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRY 253 +T + E F FQAE+++LM +IIN+ YSNK+IFLRELISN+SDALDKIR+ Sbjct: 70 KTLRSNAEKFEFQAEVSRLMDIIINSLYSNKDIFLRELISNASDALDKIRF 120 >At3g07770.1 68416.m00947 heat shock protein-related strong similarity to heat-shock protein [Secale cereale] GI:556673; contains Pfam profiles PF02518: ATPase, histidine kinase-, DNA gyrase B-, and HSP90-like domain protein, PF00183: Hsp90 protein Length = 803 Score = 91.5 bits (217), Expect = 5e-19 Identities = 46/88 (52%), Positives = 64/88 (72%), Gaps = 4/88 (4%) Frame = +1 Query: 256 SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 435 S+T+P +L I+I +K G +T+ D+GIGMT+ +LV+ LGTIA+SGT FM+AL Sbjct: 140 SVTNPELSKDAPDLDIRIYADKENGIITLTDSGIGMTRQELVDCLGTIAQSGTAKFMKAL 199 Query: 436 Q----AGADISMIGQFGVGFYSSYLVAD 507 + AG D ++IGQFGVGFYS++LVAD Sbjct: 200 KDSKDAGGDNNLIGQFGVGFYSAFLVAD 227 Score = 74.5 bits (175), Expect = 6e-14 Identities = 32/48 (66%), Positives = 41/48 (85%) Frame = +2 Query: 110 PAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRY 253 P E F +QAE+++LM LI+N+ YSNKE+FLRELISN+SDALDK+RY Sbjct: 91 PPPAEKFEYQAEVSRLMDLIVNSLYSNKEVFLRELISNASDALDKLRY 138 >At3g51350.1 68416.m05622 aspartyl protease family protein contains Eukaryotic and viral aspartyl proteases active site, PROSITE:PS00141 Length = 528 Score = 31.5 bits (68), Expect = 0.56 Identities = 17/50 (34%), Positives = 30/50 (60%), Gaps = 4/50 (8%) Frame = -1 Query: 333 SALVLVGNDLDVQLFATIE----F*RIRERSYLILSKASDELEISSRRKI 196 S + + G+ +D++LFA + F +RE +Y +L+K+ DEL RR + Sbjct: 310 SGVSVAGDPVDIRLFAKFDTGSSFTHLREPAYGVLTKSFDELVEDRRRPV 359 >At1g52670.1 68414.m05947 biotin/lipoyl attachment domain-containing protein similar to SP|Q06881 Biotin carboxyl carrier protein of acetyl-CoA carboxylase (BCCP) {Anabaena sp.}; contains Pfam profile PF00364: Biotin-requiring enzyme Length = 274 Score = 29.1 bits (62), Expect = 3.0 Identities = 19/69 (27%), Positives = 29/69 (42%) Frame = +2 Query: 110 PAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYDLSRIRQNSIVA 289 PAE E A + + S ++NT K + LIS +D+ ++L VA Sbjct: 63 PAETEAIADVKDSDETKSTVVNTHLMPKSSEVEALISEITDSSSIAEFELKLGGFRLYVA 122 Query: 290 KSCTSRSFP 316 + T S P Sbjct: 123 RKLTDESSP 131 >At5g63470.1 68418.m07968 CCAAT-box binding transcription factor Hap5a, putative Length = 250 Score = 27.9 bits (59), Expect = 6.9 Identities = 14/29 (48%), Positives = 16/29 (55%) Frame = +3 Query: 543 QYVWESSAGGSFTVRPDSGEPLGRGYKDS 629 Q VW++SAGG V SG G G DS Sbjct: 220 QSVWQNSAGGGDDVSYGSGGSSGHGNLDS 248 >At1g26170.1 68414.m03194 importin beta-2 subunit family protein similar to Importin9 isoform 1 [Mus musculus] GI:15186756; contains Pfam profile PF03810: Importin-beta N-terminal domain Length = 931 Score = 27.9 bits (59), Expect = 6.9 Identities = 13/40 (32%), Positives = 25/40 (62%) Frame = +2 Query: 137 QAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYD 256 ++E+ + S + +TF S+ +++LR I + D+ D RYD Sbjct: 259 ESELMGMFSPLWHTFESSLQVYLRSSIDGAEDSYDG-RYD 297 >At4g32140.1 68417.m04572 expressed protein Length = 394 Score = 27.5 bits (58), Expect = 9.2 Identities = 14/38 (36%), Positives = 23/38 (60%) Frame = +1 Query: 238 RQNQV*SLTDPSKLDSGKELYIKIIPNKNEGTLTIIDT 351 + N++ +LTD S ++ G L KII +GT+T D+ Sbjct: 70 KSNKIPALTDDSSVELGSPLRHKIIEMGLQGTITKKDS 107 >At3g25570.1 68416.m03180 adenosylmethionine decarboxylase family protein contains Pfam profile: PF01536 adenosylmethionine decarboxylase Length = 349 Score = 27.5 bits (58), Expect = 9.2 Identities = 14/61 (22%), Positives = 29/61 (47%) Frame = +1 Query: 373 DLVNNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSSYLVADP*LFTLNTMTTSNTC 552 D ++ G +S TK+ ++ + A+ +++ F SY++++ LF TC Sbjct: 24 DFLDPQGKSLRSLTKSQLDEILTPAECTIVSSLTNSFVDSYVLSESSLFVYPYKIIIKTC 83 Query: 553 G 555 G Sbjct: 84 G 84 >At2g04620.1 68415.m00470 cation efflux family protein potential member of the cation diffusion facilitator (CDF) family, or cation efflux (CE) family, see PMID:11500563 Length = 798 Score = 27.5 bits (58), Expect = 9.2 Identities = 12/56 (21%), Positives = 21/56 (37%), Gaps = 2/56 (3%) Frame = +3 Query: 300 HQDHSQQERGHSYD--HRYRYWYDQGRFGEQFGNHREIWY*SFHGGSSSRCRHQHD 461 H++H Q H ++ H + + + + NH H + C H HD Sbjct: 579 HEEHHQHSDSHKHEEHHEHDHHHHSHSHKHEECNHNHDHEHQSHSHNHEECNHNHD 634 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,383,835 Number of Sequences: 28952 Number of extensions: 375202 Number of successful extensions: 1032 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 980 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1020 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1516419560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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