Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30204
(756 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 183 2e-47
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 176 2e-45
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 88 1e-18
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 69 5e-13
SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr... 28 1.3
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 6.7
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 6.7
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 8.8
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 183 bits (446), Expect = 2e-47
Identities = 84/139 (60%), Positives = 97/139 (69%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 243
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 244 HVPRXXXXXXXXXXXXXXXXAHTDSCFIHSQLITGKEDAANNYARGHYTIGKEIVDLVLD 423
+VPR F QLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 424 RIRKLADQCTGLQGFLIFH 480
+IR++AD C+GLQGFL+FH
Sbjct: 121 KIRRIADNCSGLQGFLVFH 139
Score = 68.5 bits (160), Expect = 1e-12
Identities = 36/62 (58%), Positives = 47/62 (75%), Gaps = 1/62 (1%)
Frame = +3
Query: 513 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSI-PHHPTQPFGGTSDCGFXWSDN 689
+LL+ERL+++Y KKSKL+F++YPAPQVST+VVEPYNS+ H T +DC F DN
Sbjct: 151 ALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDL---ADCTF-MVDN 206
Query: 690 ES 695
ES
Sbjct: 207 ES 208
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 176 bits (429), Expect = 2e-45
Identities = 86/144 (59%), Positives = 99/144 (68%), Gaps = 5/144 (3%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 228
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 229 TGAGKHVPRXXXXXXXXXXXXXXXXAHTDSCFIHSQLITGKEDAANNYARGHYTIGKEIV 408
TG GK VPR F Q++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 409 DLVLDRIRKLADQCTGLQGFLIFH 480
D VL+RIR++AD C+GLQGFL+FH
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFH 143
Score = 69.7 bits (163), Expect = 4e-13
Identities = 35/61 (57%), Positives = 46/61 (75%)
Frame = +3
Query: 513 SLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSIPHHPTQPFGGTSDCGFXWSDNE 692
+LL+ERL+++YGKKS L+F++YPAPQVST+VVEPYNS+ T SDC F DNE
Sbjct: 155 ALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSV--LTTHATLDNSDCTF-MVDNE 211
Query: 693 S 695
+
Sbjct: 212 A 212
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 88.2 bits (209), Expect = 1e-18
Identities = 47/139 (33%), Positives = 65/139 (46%)
Frame = +1
Query: 64 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 243
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 244 HVPRXXXXXXXXXXXXXXXXAHTDSCFIHSQLITGKEDAANNYARGHYTIGKEIVDLVLD 423
+VPR + F +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 424 RIRKLADQCTGLQGFLIFH 480
+R+ A+ C LQGF + H
Sbjct: 119 VVRREAEACDALQGFQLTH 137
Score = 44.8 bits (101), Expect = 1e-05
Identities = 35/126 (27%), Positives = 54/126 (42%), Gaps = 3/126 (2%)
Frame = +3
Query: 255 AVFVDLEPTVVDEVRTGTYRQLFHPLTTYYW*GRCGQQLCPWSLHHWKGNRRFGFGQNPQ 434
AV VDLEP +D V++G + LF P Y G+ G W+ H+
Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFRPDNIIY--GQSGAGNI-WAKGHYTEGAELADAVLDV 119
Query: 435 AR*PVYRSARIPDLPL---LRWRYRLWVTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAV 605
R + L L + +LL+ ++ +Y + F++ PAP+ S V
Sbjct: 120 VRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTV 179
Query: 606 VEPYNS 623
VEPYN+
Sbjct: 180 VEPYNA 185
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 69.3 bits (162), Expect = 5e-13
Identities = 43/140 (30%), Positives = 67/140 (47%), Gaps = 2/140 (1%)
Frame = +1
Query: 67 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 246
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 247 VPRXXXXXXXXXXXXXXXXAHTDSCFIHSQLITGKE--DAANNYARGHYTIGKEIVDLVL 420
+PR S + ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 421 DRIRKLADQCTGLQGFLIFH 480
D I + AD L+GF + H
Sbjct: 120 DMIDREADGSDSLEGFSLLH 139
Score = 35.5 bits (78), Expect = 0.008
Identities = 18/39 (46%), Positives = 26/39 (66%), Gaps = 1/39 (2%)
Frame = +3
Query: 513 SLLMERLSVDYGKKSKLEFAIYPAPQ-VSTAVVEPYNSI 626
S L+ERL+ Y KK ++++P Q VS VV+PYNS+
Sbjct: 151 SFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSL 189
>SPCC576.05 |||nucear export factor|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1024
Score = 28.3 bits (60), Expect = 1.3
Identities = 13/38 (34%), Positives = 17/38 (44%)
Frame = -2
Query: 299 ADLINNSRFKIDEDSRGTCLPAPVSLKKVLKESSPPPM 186
AD+IN SR+ ID S T + L P P+
Sbjct: 455 ADIINGSRYNIDRVSPNTAFSTNIPLSLPFANKEPQPI 492
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 6.7
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 143 STASSLMARCPQTRPSGVETILSTLSSARPELAST 247
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 6.7
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 162 WPDAHRQDHRGWRRFFQHFLQR 227
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 8.8
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +2
Query: 53 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 175
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,264,063
Number of Sequences: 5004
Number of extensions: 68698
Number of successful extensions: 209
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 189
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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