BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30195 (596 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces p... 34 0.014 SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 29 0.68 SPAC16E8.16 |||transcription factor TFIIB |Schizosaccharomyces p... 29 0.68 SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein Pop1... 28 1.2 SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 28 1.2 SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosacc... 27 2.1 SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyce... 27 2.7 SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces... 27 2.7 SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|... 26 4.8 SPAC6G10.09 |||glucosidase I Gls1 |Schizosaccharomyces pombe|chr... 25 6.3 SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomy... 25 6.3 SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 25 8.4 SPCC290.02 |rpc34||DNA-directed RNA polymerase III complex subun... 25 8.4 >SPBC1683.07 |mal1||alpha-glucosidase Mal1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 579 Score = 34.3 bits (75), Expect = 0.014 Identities = 15/53 (28%), Positives = 30/53 (56%), Gaps = 1/53 (1%) Frame = +1 Query: 34 VPTYEVLDKR-DTLDEFKILFNKIKKIGVRVIVDLIPNYVFTNHTWFVQSENS 189 V Y+ +D R TL++ L + + +++++DL+ N+ H WF +S +S Sbjct: 73 VSDYKQIDSRYGTLEDLDRLMKALHERDMKLVMDLVLNHTSDQHEWFKESRSS 125 Score = 27.5 bits (58), Expect = 1.6 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +2 Query: 257 NTPAFTRSDKRQKFYLHQFCDNCADLNFGNPKVVE 361 +T A+ + Q++YLH + DLN+ PKV E Sbjct: 158 DTSAWEWDEATQEYYLHLWSVGQPDLNWETPKVRE 192 >SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schizosaccharomyces pombe|chr 2|||Manual Length = 766 Score = 28.7 bits (61), Expect = 0.68 Identities = 15/58 (25%), Positives = 25/58 (43%) Frame = -3 Query: 543 WVRFXPPRKLVPQCNPRQGNCPGPNARLPLKQFDEQLTRVINSDPTSTGHQSQALGPM 370 ++RF P + PQ + +CP N R L + + V N ++ H + PM Sbjct: 338 FLRFVNPAIISPQTSMLLDSCPSDNVRKTLATIAKIIQSVANGTSSTKTHLDVSFQPM 395 >SPAC16E8.16 |||transcription factor TFIIB |Schizosaccharomyces pombe|chr 1|||Manual Length = 340 Score = 28.7 bits (61), Expect = 0.68 Identities = 15/57 (26%), Positives = 25/57 (43%) Frame = +2 Query: 254 INTPAFTRSDKRQKFYLHQFCDNCADLNFGNPKVVEKFDMGPKACDWCPVLVGSELI 424 +N P+FT + C C + P +VE+F G C C +++G +I Sbjct: 1 MNAPSFTGLPTSM-LSVKMICSECRE---DPPNLVEEFSSGDTVCGSCGLVLGDRII 53 >SPBC1718.01 |pop1|ste16, SPBC2G2.18|F-box/WD repeat protein Pop1|Schizosaccharomyces pombe|chr 2|||Manual Length = 775 Score = 27.9 bits (59), Expect = 1.2 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = +3 Query: 195 SPTLIISSGPRDNLSNWGSKSTRQRLHEAINV 290 SP++ +SS PR N+ + ST++ L E N+ Sbjct: 25 SPSIDVSSSPRPNVERFSPCSTKKDLLEGNNI 56 >SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 488 Score = 27.9 bits (59), Expect = 1.2 Identities = 17/42 (40%), Positives = 24/42 (57%) Frame = +3 Query: 186 QHCSPTLIISSGPRDNLSNWGSKSTRQRLHEAINVRSSTCIS 311 Q+ PT+II + PRDN SKS + E +N+ SS +S Sbjct: 85 QNLVPTVIIPASPRDN----ASKSVVSK-KEVVNLSSSVALS 121 >SPBC409.06 |uch2||ubiquitin C-terminal hydrolase Uch2|Schizosaccharomyces pombe|chr 2|||Manual Length = 300 Score = 27.1 bits (57), Expect = 2.1 Identities = 16/64 (25%), Positives = 33/64 (51%) Frame = -2 Query: 367 ELLNNFGITEVKISAVVTELMQVELLTFIASCKRWRVDFEPQLDRLSLGPDEIISVGLQC 188 +++NN T+ +S ++ +++L T ++ K + P+L +LG E I +C Sbjct: 77 QVINNACATQALLSVLLNHSDEIDLGTTLSEFKDFSKTLPPELKGEALGNSEHI----RC 132 Query: 187 CSHS 176 C +S Sbjct: 133 CHNS 136 >SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 413 Score = 26.6 bits (56), Expect = 2.7 Identities = 13/47 (27%), Positives = 25/47 (53%) Frame = -3 Query: 480 PGPNARLPLKQFDEQLTRVINSDPTSTGHQSQALGPMSNFSTTLGLP 340 P P + +PL+Q + T ++ +ST + Q + P+++ S L P Sbjct: 11 PTPTSSIPLRQMSQPTTSAPSNSASSTPYSPQQV-PLTHNSYPLSTP 56 >SPBC409.18 |||phosphatidic acid phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 279 Score = 26.6 bits (56), Expect = 2.7 Identities = 10/19 (52%), Positives = 15/19 (78%) Frame = -2 Query: 70 VCHVCRALRKSALGRTRPE 14 VC +C +L K+A+GR RP+ Sbjct: 102 VCGLCVSLLKNAVGRPRPD 120 >SPAC6F6.03c |||ribosome export GTPase|Schizosaccharomyces pombe|chr 1|||Manual Length = 537 Score = 25.8 bits (54), Expect = 4.8 Identities = 15/52 (28%), Positives = 24/52 (46%) Frame = +1 Query: 127 VDLIPNYVFTNHTWFVQSENSTVALH*LFHLGQGTTCLIGVQNQHASVYTKR 282 VDL+P V + E T+A H + G LI + Q AS+++ + Sbjct: 257 VDLVPTSVAAAWVKILAKEYPTIAFHASINNSFGKGSLIQILRQFASLHSDK 308 >SPAC6G10.09 |||glucosidase I Gls1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 808 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = -3 Query: 486 NCPGPNARLPLKQFDEQLTRVINS 415 N PGPN L ++E T V+N+ Sbjct: 742 NTPGPNQNLARSIYEELRTNVVNN 765 >SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomyces pombe|chr 1|||Manual Length = 542 Score = 25.4 bits (53), Expect = 6.3 Identities = 8/18 (44%), Positives = 14/18 (77%) Frame = -3 Query: 147 VVRDQINNDSYSDLLNFV 94 V+ D +++D YSDL ++V Sbjct: 377 VINDDVSSDDYSDLFSYV 394 >SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 487 Score = 25.0 bits (52), Expect = 8.4 Identities = 8/23 (34%), Positives = 15/23 (65%) Frame = +3 Query: 177 E*EQHCSPTLIISSGPRDNLSNW 245 E HCSP +++S P+D + ++ Sbjct: 193 EMSNHCSPKIVLSLRPQDKIPDF 215 >SPCC290.02 |rpc34||DNA-directed RNA polymerase III complex subunit Rpc34|Schizosaccharomyces pombe|chr 3|||Manual Length = 301 Score = 25.0 bits (52), Expect = 8.4 Identities = 12/44 (27%), Positives = 22/44 (50%) Frame = +1 Query: 52 LDKRDTLDEFKILFNKIKKIGVRVIVDLIPNYVFTNHTWFVQSE 183 L+ ++ + K + N +KI ++ DL+P+ T WF E Sbjct: 125 LESKNLVKSIKSVKNPTRKI--YMLYDLVPSTELTGGPWFTDQE 166 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,435,160 Number of Sequences: 5004 Number of extensions: 49113 Number of successful extensions: 184 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 184 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 260219058 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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