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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS30184
         (726 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   104   1e-23
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   104   1e-23
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   104   1e-23
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    40   5e-04
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.096
SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4 family|S...    29   0.89 
SPBC582.03 |cdc13||cyclin Cdc13|Schizosaccharomyces pombe|chr 2|...    27   2.1  
SPAC630.14c |tup12||transcriptional corepressor Tup12 |Schizosac...    26   4.8  
SPAC926.06c |||leucine-rich repeat protein, unknown|Schizosaccha...    25   8.3  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  104 bits (250), Expect = 1e-23
 Identities = 65/151 (43%), Positives = 88/151 (58%), Gaps = 4/151 (2%)
 Frame = -3

Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQNLKRL---HTSIGLPHCPH 533
           +NVS +++RRG V GDSKN+PP GC  F +       I++ +  ++   ++ +   H  H
Sbjct: 311 KNVSVKDIRRGNVCGDSKNDPPMGCASFTAQ-----VIILNHPGQISAGYSPVLDCHTAH 365

Query: 532 -CLQICRNQEKVDRRTGKSTEVTQIHQV*GXNFGXPKGPIVNLVPSKPLCVESFQEFPPL 356
              +     EK+DRR+GK  E +      G         I  +VPSKP+CVE+F ++ PL
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGD------ACIAKMVPSKPMCVEAFTDYAPL 419

Query: 355 GRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 263
           GRFAVRDMRQTVAVGVIKAV     G  KVT
Sbjct: 420 GRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  104 bits (250), Expect = 1e-23
 Identities = 65/151 (43%), Positives = 88/151 (58%), Gaps = 4/151 (2%)
 Frame = -3

Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQNLKRL---HTSIGLPHCPH 533
           +NVS +++RRG V GDSKN+PP GC  F +       I++ +  ++   ++ +   H  H
Sbjct: 311 KNVSVKDIRRGNVCGDSKNDPPMGCASFTAQ-----VIILNHPGQISAGYSPVLDCHTAH 365

Query: 532 -CLQICRNQEKVDRRTGKSTEVTQIHQV*GXNFGXPKGPIVNLVPSKPLCVESFQEFPPL 356
              +     EK+DRR+GK  E +      G         I  +VPSKP+CVE+F ++ PL
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGD------ACIAKMVPSKPMCVEAFTDYAPL 419

Query: 355 GRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 263
           GRFAVRDMRQTVAVGVIKAV     G  KVT
Sbjct: 420 GRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  104 bits (250), Expect = 1e-23
 Identities = 65/151 (43%), Positives = 88/151 (58%), Gaps = 4/151 (2%)
 Frame = -3

Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQNLKRL---HTSIGLPHCPH 533
           +NVS +++RRG V GDSKN+PP GC  F +       I++ +  ++   ++ +   H  H
Sbjct: 311 KNVSVKDIRRGNVCGDSKNDPPMGCASFTAQ-----VIILNHPGQISAGYSPVLDCHTAH 365

Query: 532 -CLQICRNQEKVDRRTGKSTEVTQIHQV*GXNFGXPKGPIVNLVPSKPLCVESFQEFPPL 356
              +     EK+DRR+GK  E +      G         I  +VPSKP+CVE+F ++ PL
Sbjct: 366 IACKFAELIEKIDRRSGKKIEESPKFVKSGD------ACIAKMVPSKPMCVEAFTDYAPL 419

Query: 355 GRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 263
           GRFAVRDMRQTVAVGVIKAV     G  KVT
Sbjct: 420 GRFAVRDMRQTVAVGVIKAVEKVAPGAAKVT 450


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 39.5 bits (88), Expect = 5e-04
 Identities = 18/43 (41%), Positives = 28/43 (65%), Gaps = 1/43 (2%)
 Frame = -3

Query: 418 IVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAVN 293
           I  L    P+C+E F+++  +GRF +RD   TVAVG V+K ++
Sbjct: 620 IAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKILD 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.096
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = -3

Query: 394 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 302
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPAC227.12 |||U4/U6 x U5 tri-snRNP complex subunit Prp4
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 462

 Score = 28.7 bits (61), Expect = 0.89
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +2

Query: 506 FLISANLQAMWAVWQSNTGV*PFEILNQDG 595
           +L+SA+    W +W  +TGV   E+L Q+G
Sbjct: 279 YLVSASFDTTWRLWDVHTGV---ELLMQEG 305


>SPBC582.03 |cdc13||cyclin Cdc13|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 482

 Score = 27.5 bits (58), Expect = 2.1
 Identities = 19/66 (28%), Positives = 27/66 (40%)
 Frame = -3

Query: 709 T*RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQNLKRLHTSIGLPHCPHC 530
           T R ++RQ L    +  + +N+P     R  SS H     LV   K   +S  +P   H 
Sbjct: 2   TTRRLTRQHLLANTLGNNDENHPSNHIARAKSSLHSSENSLVNGKKATVSSTNVPKKRHA 61

Query: 529 LQICRN 512
           L    N
Sbjct: 62  LDDVSN 67


>SPAC630.14c |tup12||transcriptional corepressor Tup12
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 586

 Score = 26.2 bits (55), Expect = 4.8
 Identities = 12/33 (36%), Positives = 15/33 (45%)
 Frame = -2

Query: 443 KFWGPQGPHCQLGTFQASMCRVLPGIPTPRSFC 345
           +FW P  PH QL T Q     V+    +P   C
Sbjct: 537 QFWSPDSPHSQL-TLQGHNNSVISVAVSPNGHC 568


>SPAC926.06c |||leucine-rich repeat protein,
           unknown|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 621

 Score = 25.4 bits (53), Expect = 8.3
 Identities = 20/63 (31%), Positives = 29/63 (46%)
 Frame = -3

Query: 634 GCCRFYSSSHWGLTILVQNLKRLHTSIGLPHCPHCLQICRNQEKVDRRTGKSTEVTQIHQ 455
           GC  FY  S   L ILV +   L +  GL + P       + EK+D R    T+V +  +
Sbjct: 393 GCRTFYHISLSHLQILVLSRNHLTSLSGLENVP-------SLEKLDIRDNSITDVVEFRR 445

Query: 454 V*G 446
           + G
Sbjct: 446 LVG 448


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,963,131
Number of Sequences: 5004
Number of extensions: 61521
Number of successful extensions: 166
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 154
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 157
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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