Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30184
(726 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 95 4e-20
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 95 4e-20
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 95 4e-20
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 95 4e-20
At1g35550.1 68414.m04414 elongation factor Tu C-terminal domain-... 71 6e-13
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 38 0.005
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 33 0.25
At4g16550.1 68417.m02504 heat shock protein-related contains sim... 29 2.4
At1g55420.1 68414.m06339 DC1 domain-containing protein contains ... 29 4.1
At5g42860.1 68418.m05224 expressed protein 25 6.5
At1g55380.1 68414.m06334 DC1 domain-containing protein contains ... 28 7.2
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 95.1 bits (226), Expect = 4e-20
Identities = 60/152 (39%), Positives = 87/152 (57%), Gaps = 5/152 (3%)
Frame = -3
Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQN----LKRLHTSIGLPHCP 536
+NV+ ++L+RGYVA +SK++P G F S +++ N + + + H
Sbjct: 301 KNVAVKDLKRGYVASNSKDDPAKGAANFTSQ------VIIMNHPGQIGNGYAPVLDCHTS 354
Query: 535 H-CLQICRNQEKVDRRTGKSTEVTQIHQV*GXNFGXPKGPIVNLVPSKPLCVESFQEFPP 359
H ++ K+DRR+GK E G +V + P+KP+ VE+F E+PP
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGD------AGMVKMTPTKPMVVETFSEYPP 408
Query: 358 LGRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 263
LGRFAVRDMRQTVAVGVIK+V+ K+ G KVT
Sbjct: 409 LGRFAVRDMRQTVAVGVIKSVDKKDPTGAKVT 440
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 95.1 bits (226), Expect = 4e-20
Identities = 60/152 (39%), Positives = 87/152 (57%), Gaps = 5/152 (3%)
Frame = -3
Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQN----LKRLHTSIGLPHCP 536
+NV+ ++L+RGYVA +SK++P G F S +++ N + + + H
Sbjct: 301 KNVAVKDLKRGYVASNSKDDPAKGAANFTSQ------VIIMNHPGQIGNGYAPVLDCHTS 354
Query: 535 H-CLQICRNQEKVDRRTGKSTEVTQIHQV*GXNFGXPKGPIVNLVPSKPLCVESFQEFPP 359
H ++ K+DRR+GK E G +V + P+KP+ VE+F E+PP
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGD------AGMVKMTPTKPMVVETFSEYPP 408
Query: 358 LGRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 263
LGRFAVRDMRQTVAVGVIK+V+ K+ G KVT
Sbjct: 409 LGRFAVRDMRQTVAVGVIKSVDKKDPTGAKVT 440
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 95.1 bits (226), Expect = 4e-20
Identities = 60/152 (39%), Positives = 87/152 (57%), Gaps = 5/152 (3%)
Frame = -3
Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQN----LKRLHTSIGLPHCP 536
+NV+ ++L+RGYVA +SK++P G F S +++ N + + + H
Sbjct: 301 KNVAVKDLKRGYVASNSKDDPAKGAANFTSQ------VIIMNHPGQIGNGYAPVLDCHTS 354
Query: 535 H-CLQICRNQEKVDRRTGKSTEVTQIHQV*GXNFGXPKGPIVNLVPSKPLCVESFQEFPP 359
H ++ K+DRR+GK E G +V + P+KP+ VE+F E+PP
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGD------AGMVKMTPTKPMVVETFSEYPP 408
Query: 358 LGRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 263
LGRFAVRDMRQTVAVGVIK+V+ K+ G KVT
Sbjct: 409 LGRFAVRDMRQTVAVGVIKSVDKKDPTGAKVT 440
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 95.1 bits (226), Expect = 4e-20
Identities = 60/152 (39%), Positives = 87/152 (57%), Gaps = 5/152 (3%)
Frame = -3
Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYSSSHWGLTILVQN----LKRLHTSIGLPHCP 536
+NV+ ++L+RGYVA +SK++P G F S +++ N + + + H
Sbjct: 301 KNVAVKDLKRGYVASNSKDDPAKGAANFTSQ------VIIMNHPGQIGNGYAPVLDCHTS 354
Query: 535 H-CLQICRNQEKVDRRTGKSTEVTQIHQV*GXNFGXPKGPIVNLVPSKPLCVESFQEFPP 359
H ++ K+DRR+GK E G +V + P+KP+ VE+F E+PP
Sbjct: 355 HIAVKFSEILTKIDRRSGKEIEKEPKFLKNGD------AGMVKMTPTKPMVVETFSEYPP 408
Query: 358 LGRFAVRDMRQTVAVGVIKAVNFKEAGGGKVT 263
LGRFAVRDMRQTVAVGVIK+V+ K+ G KVT
Sbjct: 409 LGRFAVRDMRQTVAVGVIKSVDKKDPTGAKVT 440
>At1g35550.1 68414.m04414 elongation factor Tu C-terminal
domain-containing protein similar to SP|P13905
Elongation factor 1-alpha (EF-1-alpha) {Arabidopsis
thaliana}; contains Pfam profile PF03143: Elongation
factor Tu C-terminal domain
Length = 104
Score = 71.3 bits (167), Expect = 6e-13
Identities = 30/50 (60%), Positives = 40/50 (80%)
Frame = -3
Query: 418 IVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAVNFKEAGGGK 269
I+N+ P+KP+ VE++ +PPLGRFA+RDMRQTV VGVIK+V K+ G K
Sbjct: 55 IINMTPTKPMVVEAYSAYPPLGRFAIRDMRQTVGVGVIKSVVKKDPSGAK 104
Score = 48.0 bits (109), Expect = 6e-06
Identities = 19/24 (79%), Positives = 22/24 (91%)
Frame = -1
Query: 582 KISNGYTPVLDCHTAHIACKFAEI 511
+I NGYTPVLDCHT+HIA KF+EI
Sbjct: 6 QIKNGYTPVLDCHTSHIAVKFSEI 29
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 38.3 bits (85), Expect = 0.005
Identities = 14/29 (48%), Positives = 21/29 (72%)
Frame = -3
Query: 391 LCVESFQEFPPLGRFAVRDMRQTVAVGVI 305
+C+E F +FP LGRF +R +T+AVG +
Sbjct: 494 ICIEKFSDFPQLGRFTLRTEGKTIAVGKV 522
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 32.7 bits (71), Expect = 0.25
Identities = 14/33 (42%), Positives = 21/33 (63%)
Frame = -3
Query: 394 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 296
P+CVE+F E LGR +R +TVA+G + +
Sbjct: 631 PVCVETFSESRALGRVFLRSSGRTVAMGKVTRI 663
>At4g16550.1 68417.m02504 heat shock protein-related contains
similarity to Swiss-Prot:P31170 small heat shock
protein, chloroplast precursor [Arabidopsis thaliana]
Length = 743
Score = 29.5 bits (63), Expect = 2.4
Identities = 23/79 (29%), Positives = 31/79 (39%), Gaps = 2/79 (2%)
Frame = -3
Query: 703 RNVSRQELRRGYVAGDSKNNPP*GCCRFYS--SSHWGLTILVQNLKRLHTSIGLPHCPHC 530
RN +LR G P CC F+S +SH +L L H S P C C
Sbjct: 82 RNYDGTKLRPKISVGGINTKIPCKCCEFFSGFTSHMADGVLRLVLTTSHASTRRPSCT-C 140
Query: 529 LQICRNQEKVDRRTGKSTE 473
+ + D R G +T+
Sbjct: 141 ISFLGGSDGED-RVGTATD 158
>At1g55420.1 68414.m06339 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 725
Score = 28.7 bits (61), Expect = 4.1
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -1
Query: 126 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 28
I+ K C+ V LFY ++ N S FCY C+L+C
Sbjct: 619 IYLKPCHIFKVGLFYKEVEIARNDGNSRLFCYICRLRC 656
>At5g42860.1 68418.m05224 expressed protein
Length = 320
Score = 25.0 bits (52), Expect(2) = 6.5
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = -1
Query: 255 RKGHQGQEVARAVNSTIFHTTAILHSPKGVSKEKRATNS 139
R H G++ A T FH+T +L SP G +++S
Sbjct: 33 RDSHDGEKTA-----TSFHSTPVLTSPMGSPPHSHSSSS 66
Score = 21.4 bits (43), Expect(2) = 6.5
Identities = 8/13 (61%), Positives = 11/13 (84%)
Frame = -1
Query: 399 PSLYV*SPSRNSH 361
P+ +V SPSR+SH
Sbjct: 24 PAYFVQSPSRDSH 36
>At1g55380.1 68414.m06334 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 661
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -1
Query: 126 IFYKACN---VTLFYNLYKVIHNI--SETFCYDCKLKC 28
I+ K C+ V L+Y ++ N S FCY C+L+C
Sbjct: 579 IYLKPCHIFKVGLYYKEVEIARNDGNSRLFCYTCELRC 616
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,608,755
Number of Sequences: 28952
Number of extensions: 330522
Number of successful extensions: 826
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 814
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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