Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30176
(708 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 85 9e-18
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 69 7e-13
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 62 6e-11
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 62 1e-10
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 53 4e-08
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 52 8e-08
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 51 1e-07
SPCC550.02c |cwf5|ecm2|RNA-binding protein Cwf5|Schizosaccharomy... 27 2.0
SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 27 2.0
SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 6.1
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 85.0 bits (201), Expect = 9e-18
Identities = 61/187 (32%), Positives = 96/187 (51%), Gaps = 2/187 (1%)
Frame = +1
Query: 106 MSSIGTGYDLSASQFSPDGRVFQVEYAAKAVENSGTVIGLRGKDGVVFAVRS*SHPNCMN 285
MSSIGTGYDL FSPDGR+FQ EYA KAVEN+ T IG++ +DGV+ A+ +
Sbjct: 1 MSSIGTGYDLGLF-FSPDGRLFQAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLK 59
Query: 286 LAQTRGYSILMNMLAWLSQGSYQMQDKLSRLLVQRHQTTDLSMAVPKGFKNPPPTEYLMN 465
+ + + G + D + +V+R D + + + +P P + +
Sbjct: 60 PRVNNRIGSVDRHIGIATTGF--IPD--GQHIVKR--ARDEATSWRDNYGSPIPGTVIAD 113
Query: 466 EFNVHARVHT-IQCCWPYGCSVVMGHMG-QTMEGPQMYMLDPSGVSFHILGCASGKAKQA 639
+ ++ T P+G VM + EGP +YM++P+GV + G A+GK +Q
Sbjct: 114 RLGNYVQLFTCYSSVRPFG---VMSFVATYDSEGPHLYMVEPNGVYWGYNGAAAGKGRQV 170
Query: 640 AKNRVGK 660
A+N + K
Sbjct: 171 ARNELEK 177
Score = 66.5 bits (155), Expect = 4e-12
Identities = 28/59 (47%), Positives = 40/59 (67%)
Frame = +3
Query: 246 ICCEKLITSKLYEPGANKRIFHIDEHVGMAVAGLISDARQIVETARSEASNYRSQYGSP 422
+ EK++TSKL +P N RI +D H+G+A G I D + IV+ AR EA+++R YGSP
Sbjct: 47 LALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFIPDGQHIVKRARDEATSWRDNYGSP 105
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 68.9 bits (161), Expect = 7e-13
Identities = 53/189 (28%), Positives = 93/189 (49%), Gaps = 4/189 (2%)
Frame = +1
Query: 121 TGYDLSASQFSPDGRVFQVEYAAKAVENSGTVIGLRGKDGVVFAVRS*SHPNCMNLAQTR 300
+GYD + S FSPDGR+ QVEY +AV T I LRG + +V V + P N++ +
Sbjct: 2 SGYDRALSVFSPDGRLLQVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQ 61
Query: 301 GYSILMN----MLAWLSQGSYQMQDKLSRLLVQRHQTTDLSMAVPKGFKNPPPTEYLMNE 468
+++ N A L+ + + DK +R+ Q H+ L++A P + T Y+
Sbjct: 62 KIAMVDNHVCLAFAGLNADARILIDK-ARVEAQNHK---LNLADPVSIEY--LTRYVAGV 115
Query: 469 FNVHARVHTIQCCWPYGCSVVMGHMGQTMEGPQMYMLDPSGVSFHILGCASGKAKQAAKN 648
+ + ++ P+G S ++ P++Y +P+G+ A G+A +AA+
Sbjct: 116 QQKYTQSGGVR---PFGVSTLIAGFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAARE 172
Query: 649 RVGKSLKLG 675
+ K+ K G
Sbjct: 173 YLEKNWKEG 181
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 62.5 bits (145), Expect = 6e-11
Identities = 50/179 (27%), Positives = 78/179 (43%), Gaps = 1/179 (0%)
Frame = +1
Query: 127 YDLSASQFSPDGRVFQVEYAAKAVENSGTVIGLRGKDGVVFAVRS*SHPNCMNLAQTRGY 306
Y S + FSP+G++ Q+EYA AV T +G++ DGVV A A
Sbjct: 5 YAFSLTTFSPNGKLVQIEYALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGASLEKV 64
Query: 307 SILMNMLAWLSQGSYQMQDKLSRLLVQRHQTTDLSMAVPKGFKNPPPTEYLMNEF-NVHA 483
+ + + Y R+LV + + K + PPT+ L+ E +V
Sbjct: 65 CAITPDIGMV----YSGMGPDFRVLVDKSRKV-AQTTYKKIYNEYPPTKILVQEIASVMQ 119
Query: 484 RVHTIQCCWPYGCSVVMGHMGQTMEGPQMYMLDPSGVSFHILGCASGKAKQAAKNRVGK 660
P+G S+++ M + +GP +Y +DPSG F A GK+ AAK + K
Sbjct: 120 ESTQSGGVRPFGVSLLVAGMDE--KGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEK 176
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 61.7 bits (143), Expect = 1e-10
Identities = 27/58 (46%), Positives = 39/58 (67%)
Frame = +3
Query: 255 EKLITSKLYEPGANKRIFHIDEHVGMAVAGLISDARQIVETARSEASNYRSQYGSPQG 428
EK +TS L E + +++F ID H+G A++GL +DAR I+E AR + N+R Y PQG
Sbjct: 51 EKRLTSPLMESHSVEKLFEIDSHIGCAISGLTADARTIIEHARVQTQNHRFTYDEPQG 108
Score = 57.2 bits (132), Expect = 2e-09
Identities = 48/180 (26%), Positives = 83/180 (46%), Gaps = 2/180 (1%)
Frame = +1
Query: 127 YDLSASQFSPDGRVFQVEYAAKAVENSGTVIGLRGKDGVVFAV-RS*SHPNCMNLAQTRG 303
YD + FSP+GR+FQVEYA +A++ T IG++ KD VV V + + P + + +
Sbjct: 8 YDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKL 67
Query: 304 YSILMNMLAWLSQGSYQMQDKLSRLLVQRHQTTDLSMAVPKGFKNPPPTEY-LMNEFNVH 480
+ I ++ +S + + + VQ Q + P+G ++ + L F
Sbjct: 68 FEIDSHIGCAISGLTADARTIIEHARVQT-QNHRFTYDEPQGIESTTQSICDLALRFGEG 126
Query: 481 ARVHTIQCCWPYGCSVVMGHMGQTMEGPQMYMLDPSGVSFHILGCASGKAKQAAKNRVGK 660
P+G ++++ G GPQ+Y +PSG F A G + AK+ + K
Sbjct: 127 EDGEERIMSRPFGVALLIA--GIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVK 184
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 53.2 bits (122), Expect = 4e-08
Identities = 46/187 (24%), Positives = 84/187 (44%), Gaps = 2/187 (1%)
Frame = +1
Query: 124 GYDLSASQFSPDGRVFQVEYAAKAVENSG-TVIGLRGKDGVVFAVRS*SHPNCMNLAQTR 300
G+D + + FSP+GR++QVEYA KA N+G T +G+ GK+ + ++ + +
Sbjct: 6 GFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDASTVK 65
Query: 301 GYSILMNMLAWLSQGSYQMQDKLSRLLVQRHQTTDLSMAVPKGFKNPPPTEYLMNEFNVH 480
+ + + GS + D +R V R ++ G+ P P + L
Sbjct: 66 HVFPITKGIGCVMTGS--IAD--ARAQVSRARSEAAEFEYKNGY--PMPCDVLAKRMANI 119
Query: 481 ARVHTIQCCW-PYGCSVVMGHMGQTMEGPQMYMLDPSGVSFHILGCASGKAKQAAKNRVG 657
A+V T + P G ++ + + + GP ++ LDP+G ++G + N +
Sbjct: 120 AQVSTQRAAMRPLGVAMTLVAVDDEI-GPSLFKLDPAGFYIGYKATSAGPKQTETINWLE 178
Query: 658 KSLKLGG 678
K K G
Sbjct: 179 KRFKKDG 185
Score = 41.9 bits (94), Expect = 9e-05
Identities = 20/59 (33%), Positives = 32/59 (54%)
Frame = +3
Query: 246 ICCEKLITSKLYEPGANKRIFHIDEHVGMAVAGLISDARQIVETARSEASNYRSQYGSP 422
+ +K + KL + K +F I + +G + G I+DAR V ARSEA+ + + G P
Sbjct: 48 VISQKKVPDKLIDASTVKHVFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYP 106
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 52.0 bits (119), Expect = 8e-08
Identities = 42/181 (23%), Positives = 80/181 (44%), Gaps = 1/181 (0%)
Frame = +1
Query: 127 YDLSASQFSPDGRVFQVEYAAKAVENSGTVIGLRGKDGVVFAVRS*SHPNCMNLAQTRGY 306
YD A+ +SP GR+ QVEYA +A++ +GL K V N L+ +
Sbjct: 6 YDGDATTWSPQGRLHQVEYALEAIKQGSATVGLVSKTHAVLVALK---RNAEELSSYQKK 62
Query: 307 SILMNMLAWLSQGSYQMQDKLSRLLVQRHQTTDLSMAVPKGFKNPPPTEYLMNEFNVHAR 486
I ++ ++ ++ +++ + ++ F P P LM++ A+
Sbjct: 63 LIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTL-----FTRPIPVRRLMSKVAEKAQ 117
Query: 487 VHTIQC-CWPYGCSVVMGHMGQTMEGPQMYMLDPSGVSFHILGCASGKAKQAAKNRVGKS 663
++T + PYG ++ +G GP + PSG+ LG + G Q+A+ + ++
Sbjct: 118 INTQEYGRRPYGVGFLV--IGYDESGPHLLEFQPSGLVLEYLGTSMGSRSQSARTYIERN 175
Query: 664 L 666
L
Sbjct: 176 L 176
Score = 37.9 bits (84), Expect = 0.001
Identities = 15/42 (35%), Positives = 27/42 (64%)
Frame = +3
Query: 297 KRIFHIDEHVGMAVAGLISDARQIVETARSEASNYRSQYGSP 422
K++ ID+H+G+A+AGL DAR + + EA + ++ + P
Sbjct: 61 KKLIRIDDHIGIAIAGLAPDARVLSNYMKQEALSSKTLFTRP 102
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 51.2 bits (117), Expect = 1e-07
Identities = 20/42 (47%), Positives = 31/42 (73%)
Frame = +1
Query: 127 YDLSASQFSPDGRVFQVEYAAKAVENSGTVIGLRGKDGVVFA 252
YD + FSP+GR++QVEYA +A+ ++G +G+ KDG+V A
Sbjct: 5 YDSRTTIFSPEGRLYQVEYALEAINHAGVALGIVAKDGIVLA 46
Score = 37.9 bits (84), Expect = 0.001
Identities = 19/53 (35%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = +3
Query: 246 ICCEKLITSKLYEPGAN-KRIFHIDEHVGMAVAGLISDARQIVETARSEASNY 401
+ EK +TSKL E + ++++HI +++ AVAGL +DA ++ AR Y
Sbjct: 45 LAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGLTADANILINYARRVGQQY 97
>SPCC550.02c |cwf5|ecm2|RNA-binding protein Cwf5|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 354
Score = 27.5 bits (58), Expect = 2.0
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = -2
Query: 527 TEQPYGQQHCIVCTRACTLNSFIK 456
T++P GQ+ C +C++ CT+ +IK
Sbjct: 36 TKEPLGQE-CKICSKPCTIFRWIK 58
>SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 210
Score = 27.5 bits (58), Expect = 2.0
Identities = 15/53 (28%), Positives = 21/53 (39%)
Frame = +1
Query: 91 KQCVNMSSIGTGYDLSASQFSPDGRVFQVEYAAKAVENSGTVIGLRGKDGVVF 249
KQC + S TG + G F V+Y KA +G + + VF
Sbjct: 32 KQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPSQKTVALGKKRRQADVF 84
>SPBC16C6.04 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 350
Score = 25.8 bits (54), Expect = 6.1
Identities = 12/46 (26%), Positives = 22/46 (47%)
Frame = +1
Query: 79 SKYIKQCVNMSSIGTGYDLSASQFSPDGRVFQVEYAAKAVENSGTV 216
S ++ + + + +G G +S +G V + KAVENS +
Sbjct: 196 SSFVSRAASPAMVGPGVPFYSSITGANGNVLAPSGSLKAVENSDVI 241
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,357,052
Number of Sequences: 5004
Number of extensions: 76777
Number of successful extensions: 223
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 201
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 329179816
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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