BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS30161 (676 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g01640.1 68416.m00095 GHMP kinase family protein contains GHM... 30 1.2 At2g05250.1 68415.m00553 DNAJ heat shock N-terminal domain-conta... 30 1.2 At2g05230.1 68415.m00551 DNAJ heat shock N-terminal domain-conta... 30 1.2 At4g01020.1 68417.m00137 helicase domain-containing protein / IB... 29 2.1 At5g10370.1 68418.m01203 helicase domain-containing protein / IB... 29 2.8 At4g07990.1 68417.m01280 DNAJ heat shock N-terminal domain-conta... 28 5.0 >At3g01640.1 68416.m00095 GHMP kinase family protein contains GHMP kinases putative ATP-binding protein domain, Pfam:PF00288 Length = 362 Score = 30.3 bits (65), Expect = 1.2 Identities = 14/49 (28%), Positives = 28/49 (57%) Frame = -3 Query: 221 DHSRLVQIQKLSYDVD*KI*VRQECLRILRMSIAQIHRIIS*LSSCPGS 75 DHS+LV++ L++D+ ++ ECL + + + ++ R + S GS Sbjct: 268 DHSKLVELMNLNFDIRRRM-FGDECLGAMNIEMVEVARRVGAASKFTGS 315 >At2g05250.1 68415.m00553 DNAJ heat shock N-terminal domain-containing protein contains Pfam profile PF00226 DnaJ domain Length = 706 Score = 30.3 bits (65), Expect = 1.2 Identities = 19/61 (31%), Positives = 28/61 (45%) Frame = +2 Query: 434 KGVRIGYVPSTNGILICKSHIMRDVNVIPRRLKVVRRGPEIKIGNFPNAGSSISLGKHWP 613 K V+ G+ S I S I+ VN+ LK + G + FP AG ++ K+W Sbjct: 542 KWVQYGFTKSCGHFRIRNSDIVDHVNIFSHLLKGKKTGRGGCVRIFPTAGEIWAVYKNWS 601 Query: 614 L 616 L Sbjct: 602 L 602 >At2g05230.1 68415.m00551 DNAJ heat shock N-terminal domain-containing protein contains Pfam profile PF00226 DnaJ domain Length = 706 Score = 30.3 bits (65), Expect = 1.2 Identities = 19/61 (31%), Positives = 28/61 (45%) Frame = +2 Query: 434 KGVRIGYVPSTNGILICKSHIMRDVNVIPRRLKVVRRGPEIKIGNFPNAGSSISLGKHWP 613 K V+ G+ S I S I+ VN+ LK + G + FP AG ++ K+W Sbjct: 542 KWVQYGFTKSCGHFRIRNSDIVDHVNIFSHLLKGKKTGRGGCVRIFPTAGEIWAVYKNWS 601 Query: 614 L 616 L Sbjct: 602 L 602 >At4g01020.1 68417.m00137 helicase domain-containing protein / IBR domain-containing protein / zinc finger protein-related similar to SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain, PF00097: Zinc finger, C3HC4 type (RING finger), PF01485: IBR domain Length = 1787 Score = 29.5 bits (63), Expect = 2.1 Identities = 22/57 (38%), Positives = 28/57 (49%), Gaps = 2/57 (3%) Frame = -2 Query: 660 VETKSGVTKTGIFSVS--GQCLPKLILEPAFGKLPILISGPLRTTFSLLGITLTSRM 496 V+ + V K G+ ++ G CL KL LEP GK LI G R GI L + M Sbjct: 699 VQLGAVVEKNGVLELTQEGHCLVKLGLEPKLGK---LILGCFRHRMGKEGIVLAAVM 752 >At5g10370.1 68418.m01203 helicase domain-containing protein / IBR domain-containing protein / zinc finger protein-related similar to RNA-dependent ATPase/helicase Cdc28p [Schizosaccharomyces pombe] GI:1439562; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain, weak hit to PF00097: Zinc finger, C3HC4 type (RING finger), PF01485: IBR domain Length = 1775 Score = 29.1 bits (62), Expect = 2.8 Identities = 22/57 (38%), Positives = 28/57 (49%), Gaps = 2/57 (3%) Frame = -2 Query: 660 VETKSGVTKTGIFSVS--GQCLPKLILEPAFGKLPILISGPLRTTFSLLGITLTSRM 496 V+ + V K G+ ++ G CL KL LEP GK LI G R GI L + M Sbjct: 702 VQLGAVVEKNGVHELTQEGHCLVKLGLEPKLGK---LILGCFRHRMGKEGIVLAAVM 755 >At4g07990.1 68417.m01280 DNAJ heat shock N-terminal domain-containing protein similar to SP|Q9QYI5 DnaJ homolog subfamily B member 10 Mus musculus ; contains Pfam profile PF00226 DnaJ domain Length = 347 Score = 28.3 bits (60), Expect = 5.0 Identities = 13/39 (33%), Positives = 20/39 (51%) Frame = +2 Query: 422 QKPPKGVRIGYVPSTNGILICKSHIMRDVNVIPRRLKVV 538 ++P KG I ++ T C S + R NV RR++ V Sbjct: 29 KRPNKGPTISHLSKTKNSENCSSSVRRRENVFQRRIEAV 67 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,273,637 Number of Sequences: 28952 Number of extensions: 322203 Number of successful extensions: 726 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 714 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 726 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1432596384 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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