Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30147
(417 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 129 8e-31
At5g25230.1 68418.m02991 elongation factor Tu family protein tra... 87 4e-18
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 86 8e-18
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 86 8e-18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 69 1e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 44 3e-05
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 44 3e-05
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 41 4e-04
At5g57260.1 68418.m07152 cytochrome P450 71B10 identical to cyto... 31 0.41
At5g43300.1 68418.m05292 glycerophosphoryl diester phosphodieste... 31 0.41
At5g17300.1 68418.m02026 myb family transcription factor similar... 28 2.2
At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger) fa... 28 2.2
At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger) fa... 28 2.9
At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein si... 28 2.9
At2g44700.1 68415.m05563 kelch repeat-containing F-box family pr... 28 2.9
At3g02040.1 68416.m00167 glycerophosphoryl diester phosphodieste... 27 5.1
At3g05200.1 68416.m00567 zinc finger (C3HC4-type RING finger) fa... 27 6.7
At4g08395.1 68417.m01387 hypothetical protein 26 8.9
At2g42890.2 68415.m05312 RNA recognition motif (RRM)-containing ... 26 8.9
At2g42890.1 68415.m05311 RNA recognition motif (RRM)-containing ... 26 8.9
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 129 bits (311), Expect = 8e-31
Identities = 63/87 (72%), Positives = 71/87 (81%), Gaps = 1/87 (1%)
Frame = +3
Query: 3 GLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDH-ACIPIKKSDPVVSYRE 179
GLKRLAKSDPMV C EESGEHIVAGAGELHLEICLKDL++D I KSDPVVS+RE
Sbjct: 507 GLKRLAKSDPMVVCTMEESGEHIVAGAGELHLEICLKDLQDDFMGGAEIIKSDPVVSFRE 566
Query: 180 TVAEESDQLCLSKSPNKHNRLFMKASP 260
TV + S + +SKSPNKHNRL+M+A P
Sbjct: 567 TVCDRSTRTVMSKSPNKHNRLYMEARP 593
Score = 68.5 bits (160), Expect = 2e-12
Identities = 30/56 (53%), Positives = 39/56 (69%)
Frame = +2
Query: 248 EG*PMPDGLPEDIDEGRVNSRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPN 415
E PM +GL E ID+GR+ RDD K R++ L E++ +D A+KIW FGPE TGPN
Sbjct: 590 EARPMEEGLAEAIDDGRIGPRDDPKIRSKILAEEFGWDKDLAKKIWAFGPETTGPN 645
>At5g25230.1 68418.m02991 elongation factor Tu family protein
translation Elongation Factor 2, Schizosaccharomyces
pombe, PIR:T39902
Length = 973
Score = 87.0 bits (206), Expect = 4e-18
Identities = 40/86 (46%), Positives = 59/86 (68%)
Frame = +3
Query: 3 GLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 182
GL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS+ ET
Sbjct: 606 GLRKISKSYPLAITKVEESGEHTILGTGELYLDSIIKDLRELYSEVQVKVADPVVSFCET 665
Query: 183 VAEESDQLCLSKSPNKHNRLFMKASP 260
V E S C +++PNK N+L M A P
Sbjct: 666 VVESSSMKCFAETPNKKNKLTMIAEP 691
Score = 43.6 bits (98), Expect = 5e-05
Identities = 20/53 (37%), Positives = 29/53 (54%)
Frame = +2
Query: 257 PMPDGLPEDIDEGRVNSRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPN 415
P+ GL EDI+ G V+ + + KY++D+ AR IW FGP+ G N
Sbjct: 691 PLDRGLAEDIENGVVSIDWNRVQLGDFFRTKYDWDLLAARSIWAFGPDKQGTN 743
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 86.2 bits (204), Expect = 8e-18
Identities = 39/86 (45%), Positives = 59/86 (68%)
Frame = +3
Query: 3 GLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 182
GL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS+ ET
Sbjct: 620 GLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCET 679
Query: 183 VAEESDQLCLSKSPNKHNRLFMKASP 260
V E S C +++PNK N++ M A P
Sbjct: 680 VVESSSMKCFAETPNKKNKITMIAEP 705
Score = 49.6 bits (113), Expect = 8e-07
Identities = 22/53 (41%), Positives = 31/53 (58%)
Frame = +2
Query: 257 PMPDGLPEDIDEGRVNSRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPN 415
P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GPN
Sbjct: 705 PLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPN 757
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 86.2 bits (204), Expect = 8e-18
Identities = 39/86 (45%), Positives = 59/86 (68%)
Frame = +3
Query: 3 GLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 182
GL++++KS P+ EESGEH + G GEL+L+ +KDL E ++ + +K +DPVVS+ ET
Sbjct: 620 GLRKISKSYPLAITKVEESGEHTILGTGELYLDSIMKDLRELYSEVEVKVADPVVSFCET 679
Query: 183 VAEESDQLCLSKSPNKHNRLFMKASP 260
V E S C +++PNK N++ M A P
Sbjct: 680 VVESSSMKCFAETPNKKNKITMIAEP 705
Score = 49.6 bits (113), Expect = 8e-07
Identities = 22/53 (41%), Positives = 31/53 (58%)
Frame = +2
Query: 257 PMPDGLPEDIDEGRVNSRDDFKTRARYLTEKYEYDVTEARKIWCFGPEGTGPN 415
P+ GL EDI+ G V+ + K + KY++D+ AR IW FGP+ GPN
Sbjct: 705 PLDRGLAEDIENGVVSIDWNRKQLGDFFRTKYDWDLLAARSIWAFGPDKQGPN 757
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 69.3 bits (162), Expect = 1e-12
Identities = 31/68 (45%), Positives = 46/68 (67%)
Frame = +3
Query: 3 GLKRLAKSDPMVQCINEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 182
GL+ L ++DP V+ GEH++A AGE+HLE C+KDL+E A + ++ S P+VSYRET
Sbjct: 557 GLRLLNRADPFVEITVSARGEHVLAAAGEVHLERCVKDLKERFAKVNLEVSPPLVSYRET 616
Query: 183 VAEESDQL 206
+ + L
Sbjct: 617 IEGDGSNL 624
Score = 29.5 bits (63), Expect = 0.95
Identities = 20/70 (28%), Positives = 34/70 (48%), Gaps = 1/70 (1%)
Frame = +2
Query: 209 SLKVAQQAQPSVHEG*PMPDGLPEDIDEGRVNSRDDF-KTRARYLTEKYEYDVTEARKIW 385
S+K+ + +PS+ E + L + + E V+S + K R + TE + ++IW
Sbjct: 683 SVKILESQKPSLGENVDPIEELKKQLVEAGVSSSSETEKDREKCKTEWSKL----LKRIW 738
Query: 386 CFGPEGTGPN 415
GP GPN
Sbjct: 739 ALGPREKGPN 748
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 44.4 bits (100), Expect = 3e-05
Identities = 21/66 (31%), Positives = 40/66 (60%), Gaps = 1/66 (1%)
Frame = +3
Query: 6 LKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 182
L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++RET
Sbjct: 482 LNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFRET 540
Query: 183 VAEESD 200
+ + ++
Sbjct: 541 ITQRAE 546
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 44.4 bits (100), Expect = 3e-05
Identities = 21/66 (31%), Positives = 40/66 (60%), Gaps = 1/66 (1%)
Frame = +3
Query: 6 LKRLAKSDPMVQC-INEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRET 182
L R K DP + ++ ESG+ I++G GELHL+I ++ + ++ + P V++RET
Sbjct: 482 LNRFQKEDPTFRVGLDPESGQTIISGMGELHLDIYVERMRREYK-VDATVGKPRVNFRET 540
Query: 183 VAEESD 200
+ + ++
Sbjct: 541 ITQRAE 546
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 40.7 bits (91), Expect = 4e-04
Identities = 22/64 (34%), Positives = 37/64 (57%), Gaps = 1/64 (1%)
Frame = +3
Query: 3 GLKRLAKSDPMVQCI-NEESGEHIVAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRE 179
GL +LA+ DP +EE + ++ G GELHLEI + L+ + + P V+YRE
Sbjct: 513 GLIKLAQEDPSFHFSRDEEMNQTVIEGMGELHLEIIVDRLKREFK-VEANVGAPQVNYRE 571
Query: 180 TVAE 191
++++
Sbjct: 572 SISK 575
>At5g57260.1 68418.m07152 cytochrome P450 71B10 identical to
cytochrome P450 71B10 (SP:Q9LVD2) [Arabidopsis thaliana]
Length = 1483
Score = 30.7 bits (66), Expect = 0.41
Identities = 22/58 (37%), Positives = 31/58 (53%)
Frame = -3
Query: 355 FVLFCKIPSASLEVIAGIYATLINVLWQTIRHGLAFMNRRLCLLGDFERQSWSDSSAT 182
FVLF +I S +EV +Y TLI+ + R G +NR +LGD E++ S T
Sbjct: 844 FVLFNRILSVGIEVDEFLYVTLIDGI---CRKG--NLNRAFSMLGDMEQRGIQPSILT 896
>At5g43300.1 68418.m05292 glycerophosphoryl diester
phosphodiesterase family protein contains Pfam profile
PF03009: Glycerophosphoryl diester phosphodiesterase
family
Length = 333
Score = 30.7 bits (66), Expect = 0.41
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = -2
Query: 284 CPLADHQAWASLHEQTVVLVGRL*ETELVRFLSYG 180
CP+ H + EQ V++ R+ E +L FLSYG
Sbjct: 51 CPVIFHDIFMFTQEQGVIIEKRVTEMDLHEFLSYG 85
>At5g17300.1 68418.m02026 myb family transcription factor similar to
CCA1 [Arabidopsis thaliana] GI:4090569; contains Pfam
profile PF00249: Myb-like DNA-binding domain
Length = 387
Score = 28.3 bits (60), Expect = 2.2
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = -3
Query: 196 DSSATVSRYDTTGSDFLIGMQAWSSSRSLRQISRWSSPAPAT 71
D+ + S T GS+ L + + S +RSL +S S PA T
Sbjct: 154 DTQSPTSVLSTVGSEALCSLDSSSPNRSLSPVSSASPPAALT 195
>At1g51930.1 68414.m05854 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 132
Score = 28.3 bits (60), Expect = 2.2
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3
Query: 45 INEESGEHIVAGAGELHLEICLKDLEEDH 131
I EE G G G+ ICL++ E+DH
Sbjct: 63 IKEEEGGREEEGGGKRFCPICLEEYEDDH 91
>At5g41430.1 68418.m05032 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 161
Score = 27.9 bits (59), Expect = 2.9
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +3
Query: 78 GAGELHLEICLKDLEEDHACIPIKK 152
G E+ ICL++LE+ H I IKK
Sbjct: 111 GFDEIGCSICLEELEDGHEIIRIKK 135
>At3g59570.1 68416.m06647 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 720
Score = 27.9 bits (59), Expect = 2.9
Identities = 12/39 (30%), Positives = 21/39 (53%)
Frame = -2
Query: 182 GLTVRHDRVRLLDWNASMVLLKILKTDLKMEFSSTSDNV 66
G TV+ DRV W +++ +++TD +EF N+
Sbjct: 348 GGTVKEDRVSEWLWTLHRIVVDVVRTDSHLEFYEDPGNL 386
>At2g44700.1 68415.m05563 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif;
Length = 368
Score = 27.9 bits (59), Expect = 2.9
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = +2
Query: 281 DIDEGRVNSRDDFKTRARYLTEKYEYDVTEARKIWC 388
D+D R ++ + ++ EK +V+E+++IWC
Sbjct: 294 DLDNARSVGIGEYYGKLAFIWEKPSLNVSESKEIWC 329
>At3g02040.1 68416.m00167 glycerophosphoryl diester
phosphodiesterase family protein contains Pfam profile
PF03009: Glycerophosphoryl diester phosphodiesterase
family
Length = 361
Score = 27.1 bits (57), Expect = 5.1
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = -2
Query: 284 CPLADHQAWASLHEQTVVLVGRL*ETELVRFLSYG 180
CP+ H + EQ VV R+ E L F+SYG
Sbjct: 87 CPIIFHDDFIYSEEQGVVYEKRVTEVCLSEFMSYG 121
>At3g05200.1 68416.m00567 zinc finger (C3HC4-type RING finger)
family protein (ATL6) contains Pfam profile: PF00097:
Zinc finger, C3HC4 type (RING finger)
Length = 398
Score = 26.6 bits (56), Expect = 6.7
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = +3
Query: 78 GAGELHLEICLKDLEEDHACIPIKKSDPV 164
G GEL ICL + E+D + K D V
Sbjct: 122 GKGELECAICLNEFEDDETLRLLPKCDHV 150
>At4g08395.1 68417.m01387 hypothetical protein
Length = 232
Score = 26.2 bits (55), Expect = 8.9
Identities = 13/44 (29%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
Frame = +3
Query: 96 LEICLKDLEEDHACIPIKKSDPVVSYR---ETVAEESDQLCLSK 218
L+ C++ LEEDH+ + ++ +V +T+ +ES L + K
Sbjct: 151 LKTCIETLEEDHSTVMVETCRRMVVVETCIQTLEKESSMLVVEK 194
>At2g42890.2 68415.m05312 RNA recognition motif (RRM)-containing
protein
Length = 830
Score = 26.2 bits (55), Expect = 8.9
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -3
Query: 271 TIRHGLAFMNRRLCLLGDFERQSWSDSSA 185
T+RHG F R+ LLG ++ S S+
Sbjct: 523 TVRHGFPFAERQASLLGKYQHHVGSAPSS 551
>At2g42890.1 68415.m05311 RNA recognition motif (RRM)-containing
protein
Length = 843
Score = 26.2 bits (55), Expect = 8.9
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -3
Query: 271 TIRHGLAFMNRRLCLLGDFERQSWSDSSA 185
T+RHG F R+ LLG ++ S S+
Sbjct: 536 TVRHGFPFAERQASLLGKYQHHVGSAPSS 564
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,426,866
Number of Sequences: 28952
Number of extensions: 191955
Number of successful extensions: 616
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 600
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 615
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 635399168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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