Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30144
(682 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g28140.1 68418.m03398 hypothetical protein 29 2.8
At1g02300.1 68414.m00173 cathepsin B-like cysteine protease, put... 29 2.8
At4g01610.2 68417.m00211 cathepsin B-like cysteine protease, put... 28 5.0
At4g01610.1 68417.m00210 cathepsin B-like cysteine protease, put... 28 5.0
At5g03730.2 68418.m00334 serine/threonine protein kinase (CTR1) ... 28 6.6
At5g03730.1 68418.m00333 serine/threonine protein kinase (CTR1) ... 28 6.6
At1g64180.1 68414.m07270 intracellular protein transport protein... 28 6.6
At1g30135.1 68414.m03684 expressed protein 28 6.6
At5g16780.1 68418.m01965 SART-1 family protein contains Pfam dom... 27 8.7
At4g10090.1 68417.m01651 expressed protein 27 8.7
At2g29880.1 68415.m03628 hypothetical protein 27 8.7
>At5g28140.1 68418.m03398 hypothetical protein
Length = 212
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = +2
Query: 203 TQHGLHPPTKPTRVSRNPVPRPK 271
T G PT+ TR+ RN VPRPK
Sbjct: 161 TPRGRCKPTRGTRLGRNFVPRPK 183
>At1g02300.1 68414.m00173 cathepsin B-like cysteine protease,
putative similar to cathepsin B-like cysteine proteinase
GI:609175 from [Nicotiana rustica]
Length = 379
Score = 29.1 bits (62), Expect = 2.8
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 497 EQFFRTPKFRSSCKGQNQLWSETK 568
E + TPK C +NQLW E+K
Sbjct: 228 EPTYPTPKCERKCVSRNQLWGESK 251
>At4g01610.2 68417.m00211 cathepsin B-like cysteine protease,
putative similar to cathepsin B-like cysteine proteinase
GI:609175 from [Nicotiana rustica]; contains an
unusually short, 5nt exon
Length = 359
Score = 28.3 bits (60), Expect = 5.0
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 497 EQFFRTPKFRSSCKGQNQLWSETK 568
E + TPK C N+LWSE+K
Sbjct: 208 EPAYPTPKCSRKCVSDNKLWSESK 231
>At4g01610.1 68417.m00210 cathepsin B-like cysteine protease,
putative similar to cathepsin B-like cysteine proteinase
GI:609175 from [Nicotiana rustica]; contains an
unusually short, 5nt exon
Length = 359
Score = 28.3 bits (60), Expect = 5.0
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +2
Query: 497 EQFFRTPKFRSSCKGQNQLWSETK 568
E + TPK C N+LWSE+K
Sbjct: 208 EPAYPTPKCSRKCVSDNKLWSESK 231
>At5g03730.2 68418.m00334 serine/threonine protein kinase (CTR1)
identical to serine/threonine-protein kinase CTR1
[Arabidopsis thaliana] SWISS-PROT:Q05609
Length = 821
Score = 27.9 bits (59), Expect = 6.6
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -3
Query: 368 PTIAALVWNCWTNCSWVR 315
P +AA++ CWTN W R
Sbjct: 778 PQVAAIIEGCWTNEPWKR 795
>At5g03730.1 68418.m00333 serine/threonine protein kinase (CTR1)
identical to serine/threonine-protein kinase CTR1
[Arabidopsis thaliana] SWISS-PROT:Q05609
Length = 821
Score = 27.9 bits (59), Expect = 6.6
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = -3
Query: 368 PTIAALVWNCWTNCSWVR 315
P +AA++ CWTN W R
Sbjct: 778 PQVAAIIEGCWTNEPWKR 795
>At1g64180.1 68414.m07270 intracellular protein transport protein
USO1-related similar to Rap8 (GI:2326183) [Rhynchosciara
americana]; contains weak similarity to
Swiss-Prot:P25386 intracellular protein transport
protein USO1 [Saccharomyces cerevisiae]
Length = 593
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 439 QKGKKHNYQVRLKISFKGNRTIFQNPKIQVQL 534
QKGK+ ++R + F G+R++F+ P V +
Sbjct: 20 QKGKRFIEKLRRRAVFVGHRSVFRRPSTPVHI 51
>At1g30135.1 68414.m03684 expressed protein
Length = 131
Score = 27.9 bits (59), Expect = 6.6
Identities = 17/54 (31%), Positives = 25/54 (46%)
Frame = +2
Query: 113 GSLTWSKATKTSQRKPIKLNRKRKCKEISSTQHGLHPPTKPTRVSRNPVPRPKS 274
G + +S Q + I R+ K SS+ +G PP K T N +P PK+
Sbjct: 52 GKMCFSSDVTHLQARSIISIASREMKTKSSS-NGSDPPNKSTSFHHNQLPNPKA 104
>At5g16780.1 68418.m01965 SART-1 family protein contains Pfam
domain, PF03343: SART-1 family
Length = 820
Score = 27.5 bits (58), Expect = 8.7
Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 7/69 (10%)
Frame = +1
Query: 370 LKPKDSFKTLPNNQGINSKGPGPQKGKKH--NYQVRLKISFKGN-----RTIFQNPKIQV 528
L PK++F+ L + + KGPG K +K YQ LK+ N +++ + + Q
Sbjct: 694 LTPKEAFRLLSHK--FHGKGPGKMKEEKRMKQYQEELKLKQMKNSDTPSQSVQRMREAQA 751
Query: 529 QLQGPEPVM 555
QL+ P V+
Sbjct: 752 QLKTPYLVL 760
>At4g10090.1 68417.m01651 expressed protein
Length = 262
Score = 27.5 bits (58), Expect = 8.7
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1
Query: 406 NQGINSKGPGPQKGKKHNYQVRLK 477
N+GI++ G G + K N+Q R+K
Sbjct: 226 NKGISNSGRGSSRNKLQNFQFRIK 249
>At2g29880.1 68415.m03628 hypothetical protein
Length = 308
Score = 27.5 bits (58), Expect = 8.7
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +2
Query: 407 IRESIPKARXPKRGKNTTTRSGSKSVS 487
+ E++PK + P R ++ T R+G +S S
Sbjct: 187 VNETLPKEKLPTRKRSKTNRNGDRSDS 213
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,853,327
Number of Sequences: 28952
Number of extensions: 308049
Number of successful extensions: 800
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 775
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 800
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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