Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS30143
(551 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 5e-05
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 44 7e-05
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 43 2e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 42 2e-04
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 40 0.001
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 40 0.001
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.001
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 34 0.073
At2g26430.1 68415.m03171 ania-6a type cyclin (RCY1) nearly ident... 30 1.2
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 29 1.6
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 2.1
At3g12020.1 68416.m01490 kinesin motor protein-related similar t... 29 2.1
At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family... 28 4.8
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)... 28 4.8
At4g03620.1 68417.m00497 myosin heavy chain-related contains wea... 27 6.3
At3g21880.1 68416.m02759 zinc finger (B-box type) family protein... 27 6.3
At3g15250.1 68416.m01926 expressed protein ; expression supporte... 27 6.3
At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identica... 27 6.3
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 6.3
At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 27 8.3
At1g20740.1 68414.m02598 expressed protein ; expression supporte... 27 8.3
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 44.4 bits (100), Expect = 5e-05
Identities = 25/85 (29%), Positives = 49/85 (57%), Gaps = 8/85 (9%)
Frame = +2
Query: 257 EEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 412
EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR+ LPEN + +++ +
Sbjct: 73 EEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKMEEIKASM- 131
Query: 413 SDGVLTVIAPRTPAATKNERAVPIT 487
+GVL+V P+ P +++ I+
Sbjct: 132 ENGVLSVTVPKVPEKKPEVKSIDIS 156
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 44.0 bits (99), Expect = 7e-05
Identities = 30/88 (34%), Positives = 47/88 (53%), Gaps = 8/88 (9%)
Frame = +2
Query: 257 EEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 412
EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D V++ +
Sbjct: 71 EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASM- 129
Query: 413 SDGVLTVIAPRTPAATKNERAVPITQTG 496
+GVLTV P+ A K + I +G
Sbjct: 130 ENGVLTVTVPKVEEAKKKAQVKSIDISG 157
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 42.7 bits (96), Expect = 2e-04
Identities = 26/85 (30%), Positives = 48/85 (56%), Gaps = 8/85 (9%)
Frame = +2
Query: 257 EEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 412
EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN + V++ +
Sbjct: 75 EEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKMEEVKATM- 133
Query: 413 SDGVLTVIAPRTPAATKNERAVPIT 487
+GVLTV+ P+ P +++ I+
Sbjct: 134 ENGVLTVVVPKAPEKKPQVKSIDIS 158
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 42.3 bits (95), Expect = 2e-04
Identities = 27/85 (31%), Positives = 48/85 (56%), Gaps = 8/85 (9%)
Frame = +2
Query: 257 EEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPDTVESRLS 412
EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D V++ +
Sbjct: 69 EEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAM- 127
Query: 413 SDGVLTVIAPRTPAATKNERAVPIT 487
+GVLTV P+ + +++ I+
Sbjct: 128 ENGVLTVTVPKAETKKADVKSIQIS 152
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 39.9 bits (89), Expect = 0.001
Identities = 18/75 (24%), Positives = 38/75 (50%), Gaps = 3/75 (4%)
Frame = +2
Query: 248 LFAEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYALPENCNPDTVESRLSSD 418
L E++ + D ++++G+ ++ + + R + R LP+NC D +++ L +
Sbjct: 147 LSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKDKIKAEL-KN 205
Query: 419 GVLTVIAPRTPAATK 463
GVL + P+T K
Sbjct: 206 GVLFITIPKTKVERK 220
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 39.9 bits (89), Expect = 0.001
Identities = 28/88 (31%), Positives = 45/88 (51%), Gaps = 8/88 (9%)
Frame = +2
Query: 257 EEISVKTADGYVI-VEGKH----EERQDEHGYISRQ---FTRRYALPENCNPDTVESRLS 412
EE+ V+ D V+ + G+ EE+QD + R F+R++ LPEN D V++ +
Sbjct: 69 EEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMDQVKASM- 127
Query: 413 SDGVLTVIAPRTPAATKNERAVPITQTG 496
+GVLTV P+ K + I +G
Sbjct: 128 ENGVLTVTVPKVETNKKKAQVKSIDISG 155
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 39.5 bits (88), Expect = 0.001
Identities = 25/85 (29%), Positives = 47/85 (55%), Gaps = 8/85 (9%)
Frame = +2
Query: 257 EEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPDTVESRLS 412
EE+ V+ DG ++ + G+ +EE+ D + R +F RR+ LPEN + V++ +
Sbjct: 72 EEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVEEVKASM- 130
Query: 413 SDGVLTVIAPRTPAATKNERAVPIT 487
+GVL+V P+ + ++V I+
Sbjct: 131 ENGVLSVTVPKVQESKPEVKSVDIS 155
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 33.9 bits (74), Expect = 0.073
Identities = 20/88 (22%), Positives = 44/88 (50%), Gaps = 9/88 (10%)
Frame = +2
Query: 248 LFAEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----FTRRYALPENCNPDTVE 400
L E++ + DG + ++G H+ ++ E Y S + + +LP++ + ++
Sbjct: 145 LTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLPDDAKVEDIK 204
Query: 401 SRLSSDGVLTVIAPRTPAATKNERAVPI 484
+ L + GVL ++ PRT KN + + +
Sbjct: 205 AELKN-GVLNLVIPRTEKPKKNVQEISV 231
>At2g26430.1 68415.m03171 ania-6a type cyclin (RCY1) nearly
identical to ania-6a type cyclin [Arabidopsis thaliana]
GI:13924511
Length = 416
Score = 29.9 bits (64), Expect = 1.2
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = +1
Query: 430 RDRSQDSGCHEERASCSHHSNRARSGRD 513
RDR +DSG H +++ HHS+R R RD
Sbjct: 382 RDRLKDSGGHSDKS--RHHSSRDRDYRD 407
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 29.5 bits (63), Expect = 1.6
Identities = 18/63 (28%), Positives = 29/63 (46%)
Frame = +2
Query: 257 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 436
EEI V+ D ++ + F R++ LPE+ + + + DGVLTVI
Sbjct: 49 EEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIGISAGYE-DGVLTVI 107
Query: 437 APR 445
P+
Sbjct: 108 VPK 110
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 29.1 bits (62), Expect = 2.1
Identities = 21/77 (27%), Positives = 29/77 (37%)
Frame = +2
Query: 257 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 436
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDDDDDDDD 107
Query: 437 APRTPAATKNERAVPIT 487
R KN +PI+
Sbjct: 108 DDRHSRMLKNVTELPIS 124
>At3g12020.1 68416.m01490 kinesin motor protein-related similar to
putative kinesin heavy chain GB:AAD23684 GI:4567271 from
[Arabidopsis thaliana]
Length = 1030
Score = 29.1 bits (62), Expect = 2.1
Identities = 25/95 (26%), Positives = 42/95 (44%), Gaps = 6/95 (6%)
Frame = +2
Query: 281 DGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI----APRT 448
D YV VEG HE R ++ Y + TR++ L P + S+ +V+ P T
Sbjct: 524 DLYVSVEGNHEIR--DNAYREEKKTRKHGLLNWLKPKKRDHSSSASDQSSVVKSNSTPST 581
Query: 449 P--AATKNERAVPITQTGPGPEEIKEPTAEAESHE 547
P + +++ P E++ EP + E+ E
Sbjct: 582 PQGGGSHLHTESRLSEGSPLMEQLSEPREDREALE 616
>At4g01050.1 68417.m00142 hydroxyproline-rich glycoprotein family
protein
Length = 466
Score = 27.9 bits (59), Expect = 4.8
Identities = 33/111 (29%), Positives = 46/111 (41%), Gaps = 12/111 (10%)
Frame = +2
Query: 239 RSTLFAEEI--SVKTADGYVIVEGKHEERQDEHGYISR----QFTRRYALPENCNPDTVE 400
+ LFAE+ ++K D ++ + +E DE I + Q T ALP P TV
Sbjct: 315 KKLLFAEDRKQTLKQVDEFLNTKVAPKELVDELKEIGKALLPQSTSNKALPA---PATVT 371
Query: 401 SRLSSDGVLT------VIAPRTPAATKNERAVPITQTGPGPEEIKEPTAEA 535
+ S T V P T AAT P+ + P PE + P EA
Sbjct: 372 AEAESATATTTTVDKPVPEPETVAATTTTVDKPVPEPEPVPEPVPVPAIEA 422
>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
nearly identical to SH3 domain-containing protein 1
[Arabidopsis thaliana] GI:16974676; contains Pfam
profile PF00018: SH3 domain
Length = 439
Score = 27.9 bits (59), Expect = 4.8
Identities = 16/38 (42%), Positives = 17/38 (44%)
Frame = +2
Query: 80 LRTGAYSGRSPDGRHHANDVQRLLPSVEADGDRQ*RRR 193
+RT YS D RH N RL VEA RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173
>At4g03620.1 68417.m00497 myosin heavy chain-related contains weak
similarity to Swiss-Prot:P24733 myosin heavy chain,
striated muscle [Aequipecten irradians]
Length = 342
Score = 27.5 bits (58), Expect = 6.3
Identities = 18/49 (36%), Positives = 32/49 (65%)
Frame = +2
Query: 221 IPGQLRRSTLFAEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYA 367
+P Q R L +E++ +K+ DGY ++ K+++ QDE+G + R+ R YA
Sbjct: 247 VPLQGRNKEL-SEQL-LKSIDGYRSLKRKYKDVQDENGSM-RRALRDYA 292
>At3g21880.1 68416.m02759 zinc finger (B-box type) family protein
contains similarity to zinc finger protein GB:BAA33206
from [Oryza sativa]
Length = 364
Score = 27.5 bits (58), Expect = 6.3
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = +1
Query: 418 RCADRDRSQDSGCHEERASCSHHSNRARS 504
RC D S GCH ++CS +R +S
Sbjct: 59 RCLDEKVSYCQGCHWHESNCSELGHRVQS 87
>At3g15250.1 68416.m01926 expressed protein ; expression supported
by MPSS
Length = 217
Score = 27.5 bits (58), Expect = 6.3
Identities = 16/53 (30%), Positives = 24/53 (45%)
Frame = +2
Query: 380 CNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPITQTGPGPEEIKEPTAEAE 538
CN S S+ T +PRT +T N+ PI Q P ++K P + +
Sbjct: 75 CNSTNSSSSFGSNNSSTSQSPRTSYSTSNKNNSPINQE-PLDLKLKPPISRCD 126
>At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identical
to GF14 protein phi chain GI:1493805, SP:P46077 from
[Arabidopsis thaliana]
Length = 267
Score = 27.5 bits (58), Expect = 6.3
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +1
Query: 58 RTAFAIRTSDWRLLRTIS*RPSSRQRCPEITTVRGSRWRSPIETSV 195
+ R + WR++ +I + SR +TT+R +RS IE+ +
Sbjct: 59 KNVIGARRASWRIISSIEQKEESRGNDDHVTTIRD--YRSKIESEL 102
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 6.3
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +2
Query: 257 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 433
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
>At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 858
Score = 27.1 bits (57), Expect = 8.3
Identities = 16/50 (32%), Positives = 21/50 (42%)
Frame = +2
Query: 272 KTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDG 421
+ D Y EG EE ++ R Y LPE+ + VES DG
Sbjct: 52 ENVDLYEYEEGVPEEESKKNNRYDRVDNYDYELPEDFEDENVESDDDEDG 101
>At1g20740.1 68414.m02598 expressed protein ; expression supported
by MPSS
Length = 266
Score = 27.1 bits (57), Expect = 8.3
Identities = 11/29 (37%), Positives = 15/29 (51%)
Frame = -1
Query: 95 KRQSEVLIAKAVRLVSQDEWQQRHFYFTL 9
KR EVL + R + D+W F FT+
Sbjct: 237 KRTKEVLFFERYREIFNDDWDDHDFAFTI 265
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,376,386
Number of Sequences: 28952
Number of extensions: 218416
Number of successful extensions: 772
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 771
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1043173136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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